Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,840,442 T→C intergenic (+42/‑47) PA14_RS13335 → / → PA14_RS13340 LysR substrate‑binding domain‑containing protein/sigma‑70 family RNA polymerase sigma factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,840,4420TC100.0% 2145.6 / NA 531intergenic (+42/‑47)PA14_RS13335/PA14_RS13340LysR substrate‑binding domain‑containing protein/sigma‑70 family RNA polymerase sigma factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (301/230);  total (301/230)

Only 100 of 531 total aligned reads displayed.

CCGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTTTTGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTGG  >  NC_008463/2840297‑2840588
                                                                                                                                                 |                                                                                                                                                  
  ggCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTCTTGCAAAAAGGCCGCGGCTTCTCGACaa                                                                                                                                             >  2:8477095/1‑151 (MQ=255)
      aGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCg                                                                                                                                         <  2:9298201/151‑1 (MQ=255)
          cgcgGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTc                                                                                                                                     <  1:3969345/151‑1 (MQ=255)
           gcgGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCa                                                                                                                                    >  2:6102202/1‑151 (MQ=255)
           gcgGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCa                                                                                                                                    >  2:10821642/1‑151 (MQ=255)
             ggTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCAtt                                                                                                                                  >  1:3807807/1‑151 (MQ=255)
             ggTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCAtt                                                                                                                                  >  1:11195796/1‑151 (MQ=255)
             ggTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCAtt                                                                                                                                  >  1:9676159/1‑151 (MQ=255)
              gTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTa                                                                                                                                 <  1:389846/151‑1 (MQ=255)
              gTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTa                                                                                                                                 <  1:4873569/151‑1 (MQ=255)
               tGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAt                                                                                                                                >  1:565324/1‑151 (MQ=255)
                gggTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtc                                                                                                                               >  2:6745073/1‑151 (MQ=255)
                gggTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtc                                                                                                                               >  2:11020873/1‑151 (MQ=255)
                 ggTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtca                                                                                                                              <  1:4150855/151‑1 (MQ=255)
                 ggTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtca                                                                                                                              <  1:447242/151‑1 (MQ=255)
                  gTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcat                                                                                                                             <  2:984320/151‑1 (MQ=255)
                  gTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcat                                                                                                                             >  1:3129765/1‑151 (MQ=255)
                    aTGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattc                                                                                                                           >  1:5356241/1‑151 (MQ=255)
                    aTGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattc                                                                                                                           <  1:2216539/151‑1 (MQ=255)
                     tgtgCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattca                                                                                                                          >  1:1826869/1‑151 (MQ=255)
                       tgCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCAtg                                                                                                                        <  1:10318181/151‑1 (MQ=255)
                           ggCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTCGTGCAAAAAGGCCGCGGCTTCGCGACAATGCGATTCATGATCATGCTTGTGTc                                                                                                                    >  1:10119777/1‑151 (MQ=255)
                           ggCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTc                                                                                                                    <  1:8658500/151‑1 (MQ=255)
                           ggCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTc                                                                                                                    <  2:3601479/151‑1 (MQ=255)
                             cgcgACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGc                                                                                                                  >  1:11341733/1‑151 (MQ=255)
                              gcgACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGcc                                                                                                                 <  2:9628795/151‑1 (MQ=255)
                                 acgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCg                                                                                                              >  1:2022626/1‑151 (MQ=255)
                                  cgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGa                                                                                                             <  2:7000424/151‑1 (MQ=255)
                                   gaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGAc                                                                                                            >  1:7808132/1‑151 (MQ=255)
                                      ccGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACttt                                                                                                         <  2:7209176/151‑1 (MQ=255)
                                       cGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTc                                                                                                        <  1:6150607/151‑1 (MQ=255)
                                         cATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTccc                                                                                                      >  1:704694/1‑151 (MQ=255)
                                          aTCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCa                                                                                                     <  2:322923/151‑1 (MQ=255)
                                            cgcgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGACGCGTCTTCTCTACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATg                                                                                                   >  2:4926676/1‑151 (MQ=255)
                                             gcgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGt                                                                                                  >  1:2485921/1‑151 (MQ=255)
                                             gcgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGt                                                                                                  <  1:8869821/151‑1 (MQ=255)
                                              cgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACCATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTc                                                                                                 >  2:6381439/1‑151 (MQ=255)
                                              cgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTc                                                                                                 >  1:357101/1‑151 (MQ=255)
                                                aCCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCg                                                                                               >  2:5916233/1‑151 (MQ=255)
                                                aCCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCg                                                                                               <  2:11734871/151‑1 (MQ=255)
                                                 ccGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGc                                                                                              <  1:4436059/151‑1 (MQ=255)
                                                   gcgcCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACCATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGccc                                                                                            >  1:8995132/1‑151 (MQ=255)
                                                     gcCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCg                                                                                          >  2:4213921/1‑151 (MQ=255)
                                                     gcCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCg                                                                                          <  1:3489733/151‑1 (MQ=255)
                                                     gcCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCAGACTTTCCCATGTCCGCACCg                                                                                          >  1:9923190/1‑151 (MQ=255)
                                                       cAGCGACGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATGCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGAt                                                                                        >  1:6918315/1‑151 (MQ=255)
                                                       cAGCGAAGACCTGCGGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGAt                                                                                        <  1:9219397/151‑1 (MQ=255)
                                                         gCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATcc                                                                                      >  1:4719281/1‑151 (MQ=255)
                                                          cGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCt                                                                                     <  1:7956223/151‑1 (MQ=255)
                                                           gAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTAGTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCtt                                                                                    >  2:7503706/1‑151 (MQ=255)
                                                            aaGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTtc                                                                                   >  1:3210167/1‑151 (MQ=255)
                                                             aGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTtct                                                                                  <  1:5975423/151‑1 (MQ=255)
                                                                         ctgcctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCccacca                                                                      >  2:9687475/1‑151 (MQ=255)
                                                                            cctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAACAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATcg                                                                   <  1:1542534/151‑1 (MQ=255)
                                                                              tgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATcgcg                                                                 <  2:2206464/151‑1 (MQ=255)
                                                                                 gcgAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCca                                                              <  2:2811531/151‑1 (MQ=255)
                                                                                  cgAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcat                                                             <  2:2325444/151‑1 (MQ=255)
                                                                                           ggcggcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCt                                                    >  2:5700049/1‑151 (MQ=255)
                                                                                           ggcggcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCt                                                    <  2:4644516/151‑1 (MQ=255)
                                                                                              ggcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTa                                                 >  1:1363690/1‑151 (MQ=255)
                                                                                              ggcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTa                                                 >  2:7971125/1‑151 (MQ=255)
                                                                                              ggcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTa                                                 >  1:8463480/1‑151 (MQ=255)
                                                                                                ccGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAACAGGCCGCGGCTTCTCGACCATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACa                                               <  2:10931869/151‑1 (MQ=255)
                                                                                                 cGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAg                                              >  1:8302751/1‑151 (MQ=255)
                                                                                                 cGAGTAGGGCGCCGCCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAg                                              >  1:9524089/1‑151 (MQ=255)
                                                                                                  gAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGc                                             <  1:4340846/151‑1 (MQ=255)
                                                                                                  gAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGc                                             >  1:7298782/1‑151 (MQ=255)
                                                                                                     tAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAg                                          >  2:8247890/1‑151 (MQ=255)
                                                                                                     tAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAg                                          <  2:4015035/151‑1 (MQ=255)
                                                                                                       gggCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCa                                        >  1:8099635/1‑151 (MQ=255)
                                                                                                         gcgcCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCAcc                                      <  2:9449163/151‑1 (MQ=255)
                                                                                                          cgcCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCa                                     >  1:11463522/1‑151 (MQ=255)
                                                                                                          cgcCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATACATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCa                                     >  2:1945607/1‑151 (MQ=255)
                                                                                                           gccggccgGCGGGAGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCAt                                    <  1:2869322/151‑1 (MQ=255)
                                                                                                            ccggccggCGGGTGCGATTGCAAAAATGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCAAGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCCTGCATGCGCTGTACAGCGAGCACCATg                                   <  2:1339223/151‑1 (MQ=255)
                                                                                                             cggccggcGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATgg                                  <  2:10712256/151‑1 (MQ=255)
                                                                                                              ggccggcGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCAAGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGc                                 >  2:4888205/1‑151 (MQ=255)
                                                                                                               gccggcGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCt                                >  1:634308/1‑151 (MQ=255)
                                                                                                                    cggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTg                           >  1:4210309/1‑151 (MQ=255)
                                                                                                                    cggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTg                           >  1:6736263/1‑151 (MQ=255)
                                                                                                                    cggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTg                           >  1:10912935/1‑151 (MQ=255)
                                                                                                                     gggTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGc                          >  2:448075/1‑151 (MQ=255)
                                                                                                                      ggTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTTTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCa                         >  1:2673650/1‑151 (MQ=255)
                                                                                                                       gTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAg                        >  2:8876460/1‑151 (MQ=255)
                                                                                                                        tGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGa                       >  2:4289560/1‑151 (MQ=255)
                                                                                                                        tGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCGGGTGGCAGa                       >  2:1586439/1‑151 (MQ=255)
                                                                                                                         gCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGaa                      >  1:7501755/1‑151 (MQ=255)
                                                                                                                         gCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCGGGTTGCAGac                      >  2:10282628/1‑150 (MQ=255)
                                                                                                                          cTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAAc                     >  2:4448223/1‑151 (MQ=255)
                                                                                                                          cTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAAc                     >  2:3746531/1‑151 (MQ=255)
                                                                                                                          cTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAAc                     >  1:6544863/1‑151 (MQ=255)
                                                                                                                              tGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGt                 >  2:5265643/1‑151 (MQ=255)
                                                                                                                                cAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTg               >  1:10640703/1‑151 (MQ=255)
                                                                                                                                  aaaaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTgcg             <  2:5062703/151‑1 (MQ=255)
                                                                                                                                        ccGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCaa       >  1:5609572/1‑151 (MQ=255)
                                                                                                                                         cgcgGCGTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAg      <  2:3914452/151‑1 (MQ=255)
                                                                                                                                          gcgGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGc     >  2:9168755/1‑151 (MQ=255)
                                                                                                                                           cgGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCt    >  2:2660814/1‑151 (MQ=255)
                                                                                                                                            ggCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTg   >  1:1256462/1‑151 (MQ=255)
                                                                                                                                             gCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTgg  <  2:11308671/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CCGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTTTTGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTGG  >  NC_008463/2840297‑2840588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑