Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,659,805 A→G intergenic (+45/+54) alpR → / ← PA14_RS21395 transcriptional regulator AlpR/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,659,8050AG100.0% 1473.3 / NA 379intergenic (+45/+54)alpR/PA14_RS21395transcriptional regulator AlpR/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (156/223);  total (156/223)

Only 100 of 397 total aligned reads displayed.

GCTTCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAAAAAAATCCCGGGCAAAAAAAAAGGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCCG  >  NC_008463/4659657‑4659953
                                                                                                                                                    |                                                                                                                                                    
  ttCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGaagaaaa                                                                                                                                                  <  2:142123/151‑1 (MQ=255)
  ttCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGaagaaaa                                                                                                                                                  >  2:8604445/1‑151 (MQ=255)
   tCAACAGCGACGCGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGaagaaaaa                                                                                                                                                 <  2:275991/151‑1 (MQ=255)
    cAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAAt                                                                                                                                                <  1:10261287/151‑1 (MQ=255)
      aCAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATcc                                                                                                                                              <  1:9022288/151‑1 (MQ=255)
          cgacgaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCggt                                                                                                                                          <  2:1905656/151‑2 (MQ=255)
                gCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaaga                                                                                                                                    <  1:8272255/151‑3 (MQ=255)
                 cACCCGGTCGAGCGCTACGAGCCCCAGGATGCCGCCGAGCACATCAACGTGATCGGCCGGGGGTGCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGTAAATTCCCGGGCacagac                                                                                                                                   >  1:3508554/1‑146 (MQ=255)
                  aCCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaagaaa                                                                                                                                  <  2:2451697/151‑1 (MQ=255)
                      ggACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAATGAAAAAgg                                                                                                                              >  1:6690772/1‑151 (MQ=255)
                       gACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAggg                                                                                                                             >  1:7522194/1‑151 (MQ=255)
                       gACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAggg                                                                                                                             >  2:722585/1‑151 (MQ=255)
                       gACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAggg                                                                                                                             <  2:11659423/151‑1 (MQ=255)
                        aCGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggc                                                                                                                            <  1:5747448/151‑2 (MQ=255)
                        aCGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggc                                                                                                                            <  2:1579711/151‑2 (MQ=255)
                         cGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcc                                                                                                                           <  1:11222533/151‑3 (MQ=255)
                          gAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcct                                                                                                                          <  2:6539627/151‑4 (MQ=255)
                            gcgcTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGc                                                                                                                       >  1:270440/1‑151 (MQ=255)
                            gcgcTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGc                                                                                                                       <  1:5434865/151‑1 (MQ=255)
                              gcTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAt                                                                                                                     >  2:10111770/1‑151 (MQ=255)
                               cTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAtt                                                                                                                    >  1:4223965/1‑151 (MQ=255)
                                tACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtg                                                                                                                   <  1:7283139/151‑1 (MQ=255)
                                 aCGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtgc                                                                                                                  <  1:1909340/151‑1 (MQ=255)
                                 aCGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtgc                                                                                                                  >  2:3178963/1‑151 (MQ=255)
                                 aCGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtgc                                                                                                                  >  2:10242539/1‑151 (MQ=255)
                                 aCGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAAATGGGCCTGCATtgc                                                                                                                  >  2:3541084/1‑151 (MQ=255)
                                  cGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtgct                                                                                                                 <  2:3278486/151‑1 (MQ=255)
                                  cGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtgct                                                                                                                 <  2:4028419/151‑1 (MQ=255)
                                  cGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCANtgct                                                                                                                 <  2:7699783/151‑1 (MQ=255)
                                        ccAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGc                                                                                                           <  2:5534843/151‑1 (MQ=255)
                                                ccgccgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCgg                                                                                                   <  2:1742391/151‑1 (MQ=255)
                                                ccgccgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCgg                                                                                                   <  2:6967983/151‑1 (MQ=255)
                                                  gccgttcacaTCAACGTGATCGGCCGGGTGTTCGGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTa                                                                                                 <  1:4966045/145‑1 (MQ=255)
                                                  gccgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAAATGGGCCTGCATTGCTGCAGGCCCTGTCGGTa                                                                                                 >  2:10928176/1‑151 (MQ=255)
                                                   ccgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTAt                                                                                                >  2:7050311/1‑151 (MQ=255)
                                                   ccgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTAt                                                                                                <  1:2053666/151‑1 (MQ=255)
                                                    cgAGCACATCACCGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATg                                                                                               <  2:8674889/151‑1 (MQ=255)
                                                    cgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATg                                                                                               >  1:4844343/1‑151 (MQ=255)
                                                     gAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATgg                                                                                              >  2:5101426/1‑151 (MQ=255)
                                                     gAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATgg                                                                                              >  1:9925632/1‑151 (MQ=255)
                                                     gAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATgg                                                                                              <  2:6163450/151‑1 (MQ=255)
                                                       gCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGgcg                                                                                            >  2:11387589/1‑151 (MQ=255)
                                                       gCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGgcg                                                                                            >  1:1401926/1‑151 (MQ=255)
                                                        cacaTCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGgcgc                                                                                           <  2:8924131/151‑1 (MQ=255)
                                                         acaTCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑CCAGAAAATGGGCCTGCCTTGCTGCAGGCCCTTGCGGTATGGCGCa                                                                                          >  2:9715886/1‑151 (MQ=255)
                                                            tCAACGTGATCGGCCGGGTGTTCTGGTCCTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTc                                                                                       <  1:2249763/151‑1 (MQ=255)
                                                              aaCGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCag                                                                                     <  2:4728381/151‑1 (MQ=255)
                                                                  tGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCaggagg                                                                                 >  1:6289387/1‑151 (MQ=255)
                                                                  tGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCaggagg                                                                                 >  1:6009873/1‑151 (MQ=255)
                                                                    aTCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGAt                                                                               <  1:2379248/151‑1 (MQ=255)
                                                                     tCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGAtt                                                                              <  1:8118203/151‑1 (MQ=255)
                                                                      cGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTc                                                                             <  1:17769/151‑1 (MQ=255)
                                                                       ggCCGGGTGTTCTGGTTCTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGCAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCg                                                                            <  2:4614175/151‑1 (MQ=255)
                                                                       ggCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCg                                                                            >  1:8392563/1‑151 (MQ=255)
                                                                       ggCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCg                                                                            >  1:10613238/1‑151 (MQ=255)
                                                                       ggCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCg                                                                            >  1:11589926/1‑151 (MQ=255)
                                                                       ggCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAAATGGGCCTGCATTGCTGCAGGCCCGTTCGGTATGGCGCACTCAGGAGGATTCg                                                                            >  2:6607383/1‑151 (MQ=255)
                                                                              tgtTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTCTGGCGCACTCAGGAGGATTCGACCCTcc                                                                     >  2:7917416/1‑151 (MQ=255)
                                                                              tgtTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTcc                                                                     >  2:5600159/1‑151 (MQ=255)
                                                                              tgtTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTcc                                                                     <  2:11585707/151‑1 (MQ=255)
                                                                               gtTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCg                                                                    <  1:8579405/151‑1 (MQ=255)
                                                                                  cTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCCGGAGGATTCGAACCTCCGAcc                                                                 <  2:9102628/151‑1 (MQ=255)
                                                                                     gTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCt                                                              <  2:6046460/151‑1 (MQ=255)
                                                                                      tACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTc                                                             >  1:3884515/1‑151 (MQ=255)
                                                                                      tACTCGGTGCTGGTCTGACCCCTGCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGACGACAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTc                                                             <  2:10341046/151‑1 (MQ=39)
                                                                                       aCTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCg                                                            <  1:10398855/151‑1 (MQ=255)
                                                                                           ggTGCTGGTCTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAACA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTAGGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                        <  2:9013215/151‑1 (MQ=38)
                                                                                             tGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGt                                                      >  1:7963895/1‑151 (MQ=255)
                                                                                               cTGGTCTGTCCCCTTCCCGCCCCGCCTGACGACGGGGCCGCTTTCCGGCGGAAGAAATATCCCGGTCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCTGGAGGATTAGAACCTCCGACCGCTCGGTTCGTAg                                                    <  1:291105/151‑1 (MQ=38)
                                                                                               cTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                    <  2:10594278/151‑1 (MQ=255)
                                                                                                 ggTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAATA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                  >  1:4467206/1‑151 (MQ=255)
                                                                                                 ggTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                  <  1:4086604/151‑1 (MQ=255)
                                                                                                 ggTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                  <  2:127524/151‑1 (MQ=255)
                                                                                                   tCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                <  1:10068159/151‑1 (MQ=255)
                                                                                                   tCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                <  1:7802862/151‑1 (MQ=255)
                                                                                                    cTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAg                                               >  2:3917065/1‑151 (MQ=255)
                                                                                                       aCCCCTTCCCCCCCCGCCAGACCACGGGGCCGCGTTCCGGCGGAAGAAAAAACCCGGGCAAACAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAc                                            <  1:10938311/151‑1 (MQ=37)
                                                                                                          ccgtCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGACGACATATCCCGGGC‑ATAGACACTGGGCCCGCATGGCTGCAGGCCCTTGCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActct                                         <  2:8552436/148‑1 (MQ=21)
                                                                                                            ttCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                       <  1:9691994/151‑1 (MQ=255)
                                                                                                            ttCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                       <  1:4326190/151‑1 (MQ=255)
                                                                                                            gtCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                       <  1:3688457/150‑1 (MQ=255)
                                                                                                                   cccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                <  1:10003838/151‑1 (MQ=255)
                                                                                                                   cccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                >  2:8126717/1‑151 (MQ=255)
                                                                                                                     cgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                              <  2:4241509/151‑1 (MQ=255)
                                                                                                                      gccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAAATGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                             >  2:7511456/1‑151 (MQ=255)
                                                                                                                          gacgacGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAAATGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATg                         >  1:6635216/1‑151 (MQ=255)
                                                                                                                            cgacgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAg                       <  2:6293586/151‑1 (MQ=255)
                                                                                                                            cgacgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAg                       >  1:9128744/1‑151 (MQ=255)
                                                                                                                             gacgGGGCCGCGTTCCGGCGGACGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGGTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGt                      >  2:4323814/1‑151 (MQ=25)
                                                                                                                              acgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTg                     >  1:879292/1‑151 (MQ=255)
                                                                                                                                  ggCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGATCTATGAGTGCgtg                 <  2:3439211/151‑1 (MQ=255)
                                                                                                                                   gCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCgtgt                >  1:5590412/1‑151 (MQ=255)
                                                                                                                                   gCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCGGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGACCCTCCGACCGCGCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGCGTGCgtgt                <  2:9792403/151‑1 (MQ=21)
                                                                                                                                    ccGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTc               >  2:8373750/1‑151 (MQ=255)
                                                                                                                                      gcgTTCCGGCGGAAGAAATATCCCGGGCAAAGAAAAA‑GGGCCTGCAGTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCGCGGTTCGTAGCCGCGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCgg             >  2:5412672/1‑151 (MQ=21)
                                                                                                                                          tCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCt         >  1:1550825/1‑151 (MQ=255)
                                                                                                                                            cggcggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCCGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCttt       <  1:3575167/151‑1 (MQ=255)
                                                                                                                                               cggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTc    <  1:3329338/151‑1 (MQ=255)
                                                                                                                                                ggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTcc   <  2:8265071/151‑1 (MQ=255)
                                                                                                                                                ggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTcc   <  1:6921403/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTTCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAAAAAAATCCCGGGCAAAAAAAAAGGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCCG  >  NC_008463/4659657‑4659953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑