Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,760,743 A→G L73P (CTT→CCT)  PA14_RS21850 ← HNH endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,760,7430AG100.0% 1571.7 / NA 391L73P (CTT→CCT) PA14_RS21850HNH endonuclease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (171/220);  total (171/220)

Only 100 of 411 total aligned reads displayed.

AATCTCCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAAGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCTTG  >  NC_008463/4760595‑4760890
                                                                                                                                                    |                                                                                                                                                   
aaTCTCCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAggg                                                                                                                                                   <  1:7566812/151‑1 (MQ=255)
   ctcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCa                                                                                                                                                <  2:6908544/151‑1 (MQ=255)
    tcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTATTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAg                                                                                                                                               <  2:8368348/151‑1 (MQ=255)
      ccGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTtcagggtcaggg                                                                                                                                             >  1:8452238/1‑151 (MQ=255)
       cGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTcagggtcagggt                                                                                                                                            >  2:9817844/1‑151 (MQ=255)
       cGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTcagggtcagggt                                                                                                                                            <  1:3682792/151‑1 (MQ=255)
         gACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTaa                                                                                                                                          <  1:4160486/151‑1 (MQ=255)
          ccaccacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTaaa                                                                                                                                         <  2:9017737/150‑1 (MQ=255)
          acacCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTaaa                                                                                                                                         >  2:8188186/1‑151 (MQ=255)
           caccacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAAc                                                                                                                                        <  1:7810007/151‑1 (MQ=255)
             ccacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCt                                                                                                                                      >  1:3115953/1‑151 (MQ=255)
              cacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCtt                                                                                                                                     <  1:184130/151‑1 (MQ=255)
              cacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCtt                                                                                                                                     <  1:6824032/151‑1 (MQ=255)
              cacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCtt                                                                                                                                     <  2:1381461/151‑1 (MQ=255)
              cacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCtt                                                                                                                                     >  2:3919916/1‑151 (MQ=255)
               acTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTg                                                                                                                                    <  1:5650897/151‑1 (MQ=255)
                  aCGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCg                                                                                                                                 >  1:7033989/1‑151 (MQ=255)
                   cGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGa                                                                                                                                <  2:10225799/151‑1 (MQ=255)
                      cAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATAc                                                                                                                             >  1:6484333/1‑151 (MQ=255)
                            ttGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCaa                                                                                                                       >  1:663496/1‑151 (MQ=255)
                                tGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATAcc                                                                                                                   <  1:10821255/151‑1 (MQ=255)
                                aggCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCGTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATAcc                                                                                                                   <  2:10317864/150‑1 (MQ=255)
                                  gCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCaa                                                                                                                 <  1:10369946/151‑1 (MQ=255)
                                    cTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAg                                                                                                               <  2:2111222/151‑1 (MQ=255)
                                          tGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGc                                                                                                         <  1:11791/151‑1 (MQ=255)
                                          tGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGc                                                                                                         >  1:10118156/1‑151 (MQ=255)
                                              ggAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCt                                                                                                     >  1:9434425/1‑151 (MQ=255)
                                                aaTGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGa                                                                                                   >  2:5261497/1‑151 (MQ=255)
                                                aaTGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGa                                                                                                   >  2:9213871/1‑151 (MQ=255)
                                                aaTGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGa                                                                                                   >  1:7401801/1‑151 (MQ=255)
                                                 aTGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAg                                                                                                  <  1:3356264/151‑1 (MQ=255)
                                                 aTGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAg                                                                                                  <  1:2112028/151‑1 (MQ=255)
                                                   gggCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCa                                                                                                <  2:7375318/151‑1 (MQ=255)
                                                     gCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACt                                                                                              >  2:1436964/1‑151 (MQ=255)
                                                        ctAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAg                                                                                           <  1:6148734/151‑1 (MQ=255)
                                                          aGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGgc                                                                                         >  1:3922639/1‑151 (MQ=255)
                                                          aGGTAGCTATTCCCTACTTTTGCCAGGACTGCCTGCTCAAATGGCTTAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGgc                                                                                         <  2:7164066/151‑1 (MQ=255)
                                                          aGGTAGCTATTCCCGTCTTGTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGgc                                                                                         <  2:5945737/151‑1 (MQ=255)
                                                             tAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCt                                                                                      <  2:2998802/151‑1 (MQ=255)
                                                             tAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCt                                                                                      >  2:2748623/1‑151 (MQ=255)
                                                             tAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCt                                                                                      >  1:8687540/1‑151 (MQ=255)
                                                                    tCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGc                                                                               >  1:8580208/1‑151 (MQ=255)
                                                                    tCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGc                                                                               >  2:2240733/1‑151 (MQ=255)
                                                                    tCCCTTCTATTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGc                                                                               <  2:3338004/151‑1 (MQ=255)
                                                                       cttcttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCaaa                                                                            <  2:124842/151‑1 (MQ=255)
                                                                          cttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGa                                                                         <  2:7668294/151‑1 (MQ=255)
                                                                           ttttGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGag                                                                        >  2:8820638/1‑150 (MQ=255)
                                                                               gCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTAt                                                                    >  1:11457835/1‑151 (MQ=255)
                                                                                ccAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATc                                                                   >  2:11212530/1‑151 (MQ=255)
                                                                                   ggACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGt                                                                <  2:3355150/151‑1 (MQ=255)
                                                                                      ctgcctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAg                                                             >  1:129624/1‑151 (MQ=255)
                                                                                       tgcctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGc                                                            <  2:8058266/151‑1 (MQ=255)
                                                                                         cctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCtt                                                          <  2:4533889/151‑1 (MQ=255)
                                                                                            gcTCAAATGGCTGAAAACTCTCGATCAACTCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGAGACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGcc                                                       <  2:9460777/151‑1 (MQ=255)
                                                                                            gcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGcc                                                       >  2:3230264/1‑151 (MQ=255)
                                                                                            gcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGcc                                                       <  1:246965/151‑1 (MQ=255)
                                                                                               cAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCtt                                                    <  1:5218208/151‑1 (MQ=255)
                                                                                               cAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCtt                                                    >  1:2403667/1‑151 (MQ=255)
                                                                                               cAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGGAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCtt                                                    <  1:4704432/151‑1 (MQ=255)
                                                                                                  aTGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAAACTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgta                                                 >  2:3697320/1‑151 (MQ=255)
                                                                                                    ggCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgtagt                                               <  1:3776849/151‑1 (MQ=255)
                                                                                                      cTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTaa                                             >  1:152752/1‑151 (MQ=255)
                                                                                                         aaaaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAacc                                          >  1:11541451/1‑151 (MQ=255)
                                                                                                         aaaaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAacc                                          >  1:5843005/1‑151 (MQ=255)
                                                                                                          aaaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAacca                                         <  2:6668556/151‑1 (MQ=255)
                                                                                                          aaaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAacca                                         <  1:7957406/151‑1 (MQ=255)
                                                                                                          aaaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAacca                                         <  1:4912307/151‑1 (MQ=255)
                                                                                                           aaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAaccac                                        >  2:575283/1‑151 (MQ=255)
                                                                                                              tctcGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTg                                     <  2:3583221/151‑1 (MQ=255)
                                                                                                               ctcGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGc                                    <  2:4829234/151‑1 (MQ=255)
                                                                                                                tcGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCa                                   <  1:8184398/151‑1 (MQ=255)
                                                                                                                    tCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAcccc                               <  2:4285480/151‑1 (MQ=255)
                                                                                                                    tCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAcccc                               >  1:3645858/1‑151 (MQ=255)
                                                                                                                    tCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAcccc                               <  1:2621531/151‑1 (MQ=255)
                                                                                                                      aaCGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTg                             >  1:926652/1‑151 (MQ=255)
                                                                                                                      aaCGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTg                             <  2:10978850/151‑1 (MQ=255)
                                                                                                                      aaCGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTg                             <  2:6270072/151‑1 (MQ=255)
                                                                                                                        cgcgAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGc                           >  2:1082011/1‑151 (MQ=255)
                                                                                                                           gAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTa                        >  2:1696670/1‑151 (MQ=255)
                                                                                                                           gAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTa                        >  1:2203249/1‑151 (MQ=255)
                                                                                                                            aaGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTaa                       >  2:11406640/1‑151 (MQ=255)
                                                                                                                              gagaTCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAgg                     >  1:8383241/1‑151 (MQ=255)
                                                                                                                              gagaTCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAgg                     <  1:9674118/151‑1 (MQ=255)
                                                                                                                               agaTCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGa                    >  1:507060/1‑151 (MQ=255)
                                                                                                                                 aTCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATg                  <  1:2893742/151‑1 (MQ=255)
                                                                                                                                   cACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCACTACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTAAAGGATGgc                <  2:9368881/151‑1 (MQ=255)
                                                                                                                                   cACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGgc                <  2:11385024/151‑1 (MQ=255)
                                                                                                                                    aCCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGgcg               <  2:867581/151‑1 (MQ=255)
                                                                                                                                      cTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCt             <  1:10525667/151‑1 (MQ=255)
                                                                                                                                       tttCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTg            <  2:6485868/151‑1 (MQ=255)
                                                                                                                                        ttCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGt           <  2:51017/151‑1 (MQ=255)
                                                                                                                                        ttCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGt           <  2:4619233/151‑1 (MQ=255)
                                                                                                                                         tCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTa          <  1:4476009/151‑1 (MQ=255)
                                                                                                                                            aGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCg       >  2:5556076/1‑151 (MQ=255)
                                                                                                                                             gCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGc      <  2:7870721/151‑1 (MQ=255)
                                                                                                                                              cTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGcc     >  1:10020819/1‑151 (MQ=255)
                                                                                                                                               tttCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCt    <  2:1046611/151‑1 (MQ=255)
                                                                                                                                                ttCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCAGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGACCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCtt   <  2:2916083/151‑1 (MQ=255)
                                                                                                                                                 tcagggtcagggTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCTTg  >  1:8858307/1‑151 (MQ=255)
                                                                                                                                                 tcagggtcagggTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCTTg  >  1:7214285/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AATCTCCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAAGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCTTG  >  NC_008463/4760595‑4760890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑