Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,198,405 T→C intergenic (+31/+37) PA14_RS23800 → / ← mdpA ABC transporter substrate‑binding protein/metallopeptidase MdpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,198,4050TC100.0% 1686.0 / NA 425intergenic (+31/+37)PA14_RS23800/mdpAABC transporter substrate‑binding protein/metallopeptidase MdpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (219/206);  total (219/206)

Only 100 of 432 total aligned reads displayed.

GATGCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCTTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCGCC  >  NC_008463/5198257‑5198552
                                                                                                                                                    |                                                                                                                                                    
gATGCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCctt                                                                                                                                                    <  1:7601532/151‑1 (MQ=255)
 aTGCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTg                                                                                                                                                   >  2:215495/1‑151 (MQ=255)
 aTGCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTg                                                                                                                                                   <  2:893454/151‑1 (MQ=255)
   gCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGcc                                                                                                                                                 <  1:7428380/151‑1 (MQ=255)
   gCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGcc                                                                                                                                                 <  2:11521777/151‑1 (MQ=255)
     ccTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCtt                                                                                                                                               <  1:6848516/151‑1 (MQ=255)
     ccTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCtt                                                                                                                                               <  1:2075241/151‑1 (MQ=255)
         gTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCcg                                                                                                                                           <  1:9566754/151‑1 (MQ=255)
          ttGCCGCTGGCGCATCCGTTGATGTTCGACGCGCCACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCGTCCcgc                                                                                                                                          >  2:7069380/1‑151 (MQ=255)
           tGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCcgcg                                                                                                                                         >  2:5750061/1‑151 (MQ=255)
            gCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGt                                                                                                                                        >  1:9059931/1‑151 (MQ=255)
               gCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGGgcg                                                                                                                                     >  2:7949370/1‑151 (MQ=255)
                cTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTgcgg                                                                                                                                    >  2:10085740/1‑151 (MQ=255)
                cTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTgcgg                                                                                                                                    >  2:9340065/1‑151 (MQ=255)
                 tGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTgcggc                                                                                                                                   >  2:4970347/1‑151 (MQ=255)
                    cgcATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGtt                                                                                                                                >  1:2608904/1‑151 (MQ=255)
                      cATCCGTTGATGTTCGACGCGCAACTCAAGACCGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTAt                                                                                                                              <  2:6466489/151‑1 (MQ=255)
                      cATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTAt                                                                                                                              >  2:9796186/1‑151 (MQ=255)
                      cATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTAt                                                                                                                              >  2:10816981/1‑151 (MQ=255)
                        tCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGTAGCCCTGGCCCTTGCCTTACCGCGTGCGGCGGTTTCg                                                                                                                            >  1:4342860/1‑151 (MQ=255)
                         ccGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCGTGCCTTCCCGCGTGCGGCGTTATCGt                                                                                                                           >  2:3961662/1‑151 (MQ=255)
                          cGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGtt                                                                                                                          >  2:3024090/1‑151 (MQ=255)
                          cGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGtt                                                                                                                          <  1:5855989/151‑1 (MQ=255)
                             tGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCATGCCATCCCGCGTGCGGCGTTATCGTTcgg                                                                                                                       >  1:3859395/1‑151 (MQ=255)
                              gATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTcggg                                                                                                                      >  1:4223021/1‑150 (MQ=255)
                               aTGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTcggcc                                                                                                                     >  2:3426981/1‑151 (MQ=255)
                                        gcgcAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAgcc                                                                                                            >  2:3082241/1‑151 (MQ=255)
                                            aacgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGccgccg                                                                                                        <  1:10517005/151‑1 (MQ=255)
                                             acgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTAGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCAAGAGGGAACCACAGGCCCATGCCATCCCCCGGGCGGGGGTATAGTTCGGGCGGCTCACGCGCCGa                                                                                                       >  1:10834644/1‑151 (MQ=255)
                                               gcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAgg                                                                                                     >  1:3357254/1‑151 (MQ=255)
                                               gcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAgg                                                                                                     >  2:6592053/1‑151 (MQ=255)
                                                caaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGGGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGc                                                                                                    >  1:10416879/1‑151 (MQ=255)
                                                      cGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAgg                                                                                              <  2:2937577/151‑1 (MQ=255)
                                                      cGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGGGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAgg                                                                                              >  2:7129106/1‑151 (MQ=255)
                                                       gacAGCGGCGACCGCATGAGCCCGAGGTCGGCGCGGGACGTGGCGCGGGTGCAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTGCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAggg                                                                                             <  2:10409878/149‑1 (MQ=255)
                                                       gTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAggg                                                                                             <  1:3746193/151‑1 (MQ=255)
                                                        tCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCAGGCCCTTGCCTTCCCGCGTGCGGCGTTTTCGTTAGGCCGGCTCAGCCGCCGAGGCCGAAGGGa                                                                                            >  1:5497130/1‑151 (MQ=255)
                                                         cAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGAt                                                                                           <  1:5921147/151‑1 (MQ=255)
                                                          aGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCAGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTAGGCCGGCTCAGCCGCCGAGGCCGAAGGGAtc                                                                                          >  1:8079460/1‑151 (MQ=255)
                                                          aGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCAGGCCCATGCCTTCCCGCGTGCGGCGTTATCGTTAGGCCGGCTCAGCCGCCGAGGCCGAAGGGAtc                                                                                          >  1:1735768/1‑151 (MQ=255)
                                                            cGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGAtcgt                                                                                        <  1:2694983/151‑1 (MQ=255)
                                                            cGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGAtcgt                                                                                        >  1:7839481/1‑151 (MQ=255)
                                                             ggCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGAtcgtc                                                                                       <  1:2451544/151‑1 (MQ=255)
                                                                tACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGGTCAGCCGCCGAGAGCGAAGGGATCGGCGAt                                                                                    >  1:9376130/1‑151 (MQ=255)
                                                                tACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGAt                                                                                    >  1:2867153/1‑151 (MQ=255)
                                                                tACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCGTTCCTTCCCCCGTGCGGCGGTTTCGTTCGGCCCGCGCAGCCGCCGAGGCCGCCGGGCTCGGCGAt                                                                                    >  1:8922784/1‑151 (MQ=255)
                                                                  ccGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATgg                                                                                  <  1:520098/151‑1 (MQ=255)
                                                                  ccGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATgg                                                                                  <  2:7582788/151‑1 (MQ=255)
                                                                    gCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATggac                                                                                <  1:9943603/151‑1 (MQ=255)
                                                                     cATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATggacg                                                                               <  1:3045881/151‑1 (MQ=255)
                                                                        gAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCAGGCCCGTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGGCGAAGGGATAGTCGATggacgggc                                                                            >  1:10163343/1‑149 (MQ=255)
                                                                           cccGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCAGGCCCATTGCTTCCCCCGGGCGGCGGTATGGTTCGGGCGGCGCAGCCGCCGCGGCCCAAGGGATCGTCGATGGACGGTCTC‑g                                                                        >  1:9924936/1‑151 (MQ=255)
                                                                             cGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGt                                                                      <  2:6953461/151‑1 (MQ=255)
                                                                               aTGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTg                                                                    <  1:102257/151‑1 (MQ=255)
                                                                               aTGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCGGGCCCTTGCCATCCCGCGTGCGGCGGTTTCGTTCGGCCGGCACAGCCGCCGAGGGCGAAGGGATCGTCGATGGACCGGCAC‑GGTTg                                                                    >  1:10047807/1‑151 (MQ=255)
                                                                                tGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTgg                                                                   >  2:360777/1‑151 (MQ=255)
                                                                                 gTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTggg                                                                  <  1:4727745/151‑1 (MQ=255)
                                                                                 gTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTggg                                                                  <  2:9240805/151‑1 (MQ=255)
                                                                                 gTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGGTggg                                                                  >  1:6775200/1‑151 (MQ=255)
                                                                                  tCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGt                                                                 <  1:11612638/151‑1 (MQ=255)
                                                                                  tCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGt                                                                 >  2:3759651/1‑151 (MQ=255)
                                                                                          ggACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCCGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGGTGGGTGTACCAGc                                                         >  2:4315805/1‑151 (MQ=255)
                                                                                           gACATTTCGCGGGAGAAGCTGGACTCATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGAGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGAGCGTCGATGGACGGACGC‑GGGTGGGTGAACCAGCg                                                        >  2:9430589/1‑151 (MQ=255)
                                                                                            aCTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGGACCCCAGGCCCGTTCCATCCCGCGTGCGGCGTTATCGTTCGGCCGGCGCACCCCCCGAGGCCGGAGGGGTAGTCGATGGACGGACTC‑GGGTGGGGGAAACAGCGt                                                       >  1:9122557/1‑151 (MQ=255)
                                                                                            aCTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGCAGCCCTGGCCCTTGCCTTCCCCCGGGCGGCGGTATCGTTCGGCCGGGGCAGCCGCCGCGGCCGAAGGGGTCGTCGATGGAAGGACGCGGGTTGGGTG‑ACCAGCgg                                                       >  1:11285876/1‑150 (MQ=255)
                                                                                            aCTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGt                                                       <  1:1232176/151‑1 (MQ=255)
                                                                                              ttttCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTgg                                                     <  1:502031/151‑1 (MQ=255)
                                                                                                ttCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGATGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGtc                                                   <  2:6780575/151‑1 (MQ=255)
                                                                                                   gcgGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGGGAACCAGCGTGGtcctt                                                >  2:7159077/1‑151 (MQ=255)
                                                                                                    cgGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGtccttc                                               >  2:7303488/1‑151 (MQ=255)
                                                                                                      ggTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCGTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGtccttcct                                             <  2:10547477/151‑1 (MQ=255)
                                                                                                       gTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTtcctc                                            <  1:3530795/151‑1 (MQ=255)
                                                                                                          aaGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGt                                         <  1:9834639/151‑1 (MQ=255)
                                                                                                              tGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATg                                     <  1:9496605/151‑1 (MQ=255)
                                                                                                               ggACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCGTCCCGCGTGCGGCGGTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGGCGATGGACGGACGC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGt                                    >  2:4106997/1‑151 (MQ=255)
                                                                                                                gACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTa                                   >  2:1857713/1‑151 (MQ=255)
                                                                                                                       cGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGGTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGGGGTCCTTCCTCCGTCAAGTAGAAGTGa                            >  2:1097666/1‑151 (MQ=255)
                                                                                                                         cTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGAtc                          <  2:5912063/151‑1 (MQ=255)
                                                                                                                          tGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGAtcc                         <  2:8724988/151‑1 (MQ=255)
                                                                                                                                 cACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGc                  <  1:1440861/151‑1 (MQ=255)
                                                                                                                                 cACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGc                  >  2:11718275/1‑151 (MQ=255)
                                                                                                                                 cACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGc                  <  1:4514892/151‑1 (MQ=255)
                                                                                                                                  aCGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCCGCGGGGTCCTTCCTCCGTCATGTAGCAGTGATCCTCCAGGCg                 >  2:9902615/1‑151 (MQ=255)
                                                                                                                                   cgcgGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGa                >  1:1818051/1‑151 (MQ=255)
                                                                                                                                   cgcgGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGa                <  1:8642446/151‑1 (MQ=255)
                                                                                                                                    gcgGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGaa               >  2:11231140/1‑151 (MQ=255)
                                                                                                                                      gggAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATg             >  2:2472973/1‑151 (MQ=255)
                                                                                                                                      gggAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATg             >  1:7093301/1‑151 (MQ=255)
                                                                                                                                      gggAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATg             >  2:621707/1‑151 (MQ=255)
                                                                                                                                       ggAAGCCCTGGCCCGTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGc            <  1:6151048/151‑1 (MQ=255)
                                                                                                                                        gAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGcc           >  1:5561661/1‑151 (MQ=255)
                                                                                                                                          aGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGa         >  2:7889530/1‑151 (MQ=255)
                                                                                                                                             ccTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACt      <  1:8361738/151‑1 (MQ=255)
                                                                                                                                             ccTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACt      >  2:10609002/1‑151 (MQ=255)
                                                                                                                                             ccTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACt      <  2:1501432/151‑1 (MQ=255)
                                                                                                                                              cTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGACCCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTc     >  2:8454420/1‑151 (MQ=255)
                                                                                                                                              cTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTc     >  1:6555757/1‑151 (MQ=255)
                                                                                                                                               tGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCg    >  2:9046349/1‑151 (MQ=255)
                                                                                                                                               tGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCg    <  1:4006568/151‑1 (MQ=255)
                                                                                                                                                 gCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCGcc  >  2:4574087/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATGCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCTTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTC‑GGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCGCC  >  NC_008463/5198257‑5198552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑