Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,530,315 A→G K229K (AAA→AAG)  PA14_RS25330 → iron ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,530,3150AG100.0% 1564.8 / NA 389K229K (AAA→AAG) PA14_RS25330iron ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (203/186);  total (203/186)

Only 100 of 419 total aligned reads displayed.

TCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAAAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGAG  >  NC_008463/5530167‑5530462
                                                                                                                                                    |                                                                                                                                                   
tCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACCACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTgaagaa                                                                                                                                                   <  1:9988153/151‑1 (MQ=255)
tCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTgaagaa                                                                                                                                                   <  1:3603920/151‑1 (MQ=255)
 ccaGGGCCAGGAGGAAGCCGAGGAGTGGCTGACCTGCCTGAAGGCCTTCGGCTAGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGaagaag                                                                                                                                                  <  2:3455468/149‑1 (MQ=255)
  aaGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaagg                                                                                                                                                 <  1:6644474/151‑1 (MQ=255)
   aGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaagga                                                                                                                                                <  1:1649635/151‑1 (MQ=255)
     ggCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaaggaaa                                                                                                                                              >  2:4921304/1‑151 (MQ=255)
      gCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaaggaaaa                                                                                                                                             <  1:4226168/151‑1 (MQ=255)
        cAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaaggaaaagg                                                                                                                                           >  2:7367930/1‑151 (MQ=255)
            aggaAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGa                                                                                                                                       >  2:11075078/1‑151 (MQ=255)
               aaGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGtt                                                                                                                                    <  1:9848059/151‑1 (MQ=255)
                aGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTg                                                                                                                                   <  1:9753366/151‑1 (MQ=255)
                 gCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGa                                                                                                                                  >  1:11434190/1‑151 (MQ=255)
                   cGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAAc                                                                                                                                >  2:4181280/1‑151 (MQ=255)
                    gAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACt                                                                                                                               >  2:6438193/1‑151 (MQ=255)
                    gAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACt                                                                                                                               >  1:1223822/1‑151 (MQ=255)
                      ggATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTcg                                                                                                                             >  2:9535280/1‑151 (MQ=255)
                          tGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGcc                                                                                                                         <  1:9500065/151‑1 (MQ=255)
                           ggCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCt                                                                                                                        >  2:6868248/1‑151 (MQ=255)
                             cTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGc                                                                                                                      >  1:1968964/1‑151 (MQ=255)
                             cTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGc                                                                                                                      >  2:5039545/1‑151 (MQ=255)
                                 ccGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCacta                                                                                                                  >  1:473222/1‑151 (MQ=255)
                                 ccGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCacta                                                                                                                  <  1:10882243/151‑1 (MQ=255)
                                  cGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAACGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAAGTCGCGCCTGCactac                                                                                                                 >  2:3160514/1‑151 (MQ=255)
                                  cGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCactac                                                                                                                 <  1:6330349/151‑1 (MQ=255)
                                     ccTGAAGGCCTACGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTc                                                                                                              <  2:5600770/151‑1 (MQ=255)
                                       tGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCgg                                                                                                            >  2:2079299/1‑151 (MQ=255)
                                        gAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGc                                                                                                           >  1:7611056/1‑151 (MQ=255)
                                          aGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCaa                                                                                                         >  2:8521586/1‑151 (MQ=255)
                                              cTTCTGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAg                                                                                                     <  2:10909405/151‑1 (MQ=255)
                                               ttCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAgg                                                                                                    >  1:10504914/1‑151 (MQ=255)
                                                  ggCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGAcc                                                                                                 <  2:8327002/151‑1 (MQ=255)
                                                   gCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGAccc                                                                                                >  1:2635519/1‑151 (MQ=255)
                                                        aTCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcg                                                                                           >  2:1788237/1‑151 (MQ=255)
                                                            acacCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCt                                                                                       >  2:7567295/1‑151 (MQ=255)
                                                            acacCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCt                                                                                       >  2:709279/1‑151 (MQ=255)
                                                               ccAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGt                                                                                    >  1:2918095/1‑151 (MQ=255)
                                                               ccAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACGACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGt                                                                                    <  1:6950908/151‑1 (MQ=255)
                                                                  acaacGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCAc                                                                                 >  2:793011/1‑151 (MQ=255)
                                                                   caacGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCAcc                                                                                <  1:2259045/151‑1 (MQ=255)
                                                                        tCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGtctc                                                                           <  1:7189656/151‑1 (MQ=255)
                                                                          aCCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCg                                                                         >  1:10372657/1‑151 (MQ=255)
                                                                          aCCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCg                                                                         <  1:11307690/151‑1 (MQ=255)
                                                                            cGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgc                                                                       >  2:8875376/1‑151 (MQ=255)
                                                                             gCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgcg                                                                      <  1:7912142/151‑1 (MQ=255)
                                                                             gCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgcg                                                                      <  1:8798857/151‑1 (MQ=255)
                                                                              ccATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgcgc                                                                     <  1:5171853/151‑1 (MQ=255)
                                                                               cATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGcgcc                                                                    <  2:10249442/151‑1 (MQ=255)
                                                                                aTGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGcgccg                                                                   <  1:11118258/151‑1 (MQ=255)
                                                                                  gAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCa                                                                 <  1:4649233/151‑1 (MQ=255)
                                                                                   aaaGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAg                                                                >  1:5798388/1‑151 (MQ=255)
                                                                                   aaaGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAg                                                                >  2:1229710/1‑151 (MQ=255)
                                                                                         gTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCa                                                          >  2:7326980/1‑151 (MQ=255)
                                                                                         gTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCa                                                          >  1:5453867/1‑151 (MQ=255)
                                                                                           ggAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAg                                                        <  1:8171738/151‑1 (MQ=255)
                                                                                             agaACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTc                                                      >  2:9293419/1‑151 (MQ=255)
                                                                                               aaCGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCa                                                    <  2:2216387/151‑1 (MQ=255)
                                                                                               aaCGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCa                                                    <  2:7801234/151‑1 (MQ=255)
                                                                                                aCGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCag                                                   <  1:8548926/151‑1 (MQ=255)
                                                                                                 cGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCagc                                                  <  2:5973385/151‑1 (MQ=255)
                                                                                                    cGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCagcaag                                               <  1:4558861/151‑1 (MQ=255)
                                                                                                        gTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCAcc                                           >  2:3243121/1‑151 (MQ=255)
                                                                                                        gTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCAcc                                           <  1:9276062/151‑1 (MQ=255)
                                                                                                         tCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCAccc                                          <  1:2124369/151‑1 (MQ=255)
                                                                                                         tCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCAccc                                          <  1:3315139/151‑1 (MQ=255)
                                                                                                         tCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCAccc                                          <  2:5512149/151‑1 (MQ=255)
                                                                                                           gACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCa                                        >  2:5127563/1‑151 (MQ=255)
                                                                                                               tGGCGCTGAACAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGa                                    <  1:3066213/151‑1 (MQ=255)
                                                                                                                ggCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGaa                                   >  1:11725647/1‑151 (MQ=255)
                                                                                                                ggCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGaa                                   >  2:6931409/1‑151 (MQ=255)
                                                                                                                 gcgcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAg                                  <  2:10452737/151‑1 (MQ=255)
                                                                                                                 gcgcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAg                                  >  1:1470174/1‑151 (MQ=255)
                                                                                                                 gcgcTGATCAACAACTACTACAGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAg                                  <  1:7431144/151‑1 (MQ=255)
                                                                                                                    cTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGccc                               <  2:9197111/151‑1 (MQ=255)
                                                                                                                     agATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCca                              <  1:593962/150‑1 (MQ=255)
                                                                                                                      gATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcag                             <  2:9787128/151‑1 (MQ=255)
                                                                                                                       aTCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcagc                            >  1:4162006/1‑151 (MQ=255)
                                                                                                                       aTCAACAACGACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcagc                            <  1:4323079/151‑1 (MQ=255)
                                                                                                                         caacaaCTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcagcag                          <  2:4784767/151‑1 (MQ=255)
                                                                                                                         caacaaCTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcagcag                          >  2:2438819/1‑151 (MQ=255)
                                                                                                                           acaacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGtt                        <  2:3894206/151‑1 (MQ=255)
                                                                                                                            caacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtc                       <  1:5605259/151‑1 (MQ=255)
                                                                                                                             aacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcg                      >  2:4548290/1‑151 (MQ=255)
                                                                                                                             aacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcg                      <  1:905487/151‑1 (MQ=255)
                                                                                                                                tactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgtcg                   <  1:10024433/151‑1 (MQ=255)
                                                                                                                                tactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgtcg                   <  1:7354230/151‑1 (MQ=255)
                                                                                                                                 actactGGTACACCCTGAAGACGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCATGTCCCGCAAGCACCCCAGGGAAGCCCAGCTGTGCGTCGc                  >  2:7188250/1‑151 (MQ=255)
                                                                                                                                 actactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGc                  >  1:3686752/1‑151 (MQ=255)
                                                                                                                                 actactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGc                  >  1:3469442/1‑151 (MQ=255)
                                                                                                                                   tactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCt                >  1:3192322/1‑151 (MQ=255)
                                                                                                                                    actGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCCCTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCtt               <  1:4904019/151‑1 (MQ=255)
                                                                                                                                      tGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCa             >  2:681830/1‑151 (MQ=255)
                                                                                                                                      tGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCa             <  1:2529132/151‑1 (MQ=255)
                                                                                                                                           cacCCTGAAGTAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCCGCAAGCACCCCAGGGAAGCCCAGCAGTGCGTCGCCTTCATGCTc        >  2:11712223/1‑151 (MQ=255)
                                                                                                                                           cacCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTc        >  1:8635949/1‑151 (MQ=255)
                                                                                                                                           cacCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTc        >  1:3857107/1‑151 (MQ=255)
                                                                                                                                           cacCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTc        <  1:11075078/151‑1 (MQ=255)
                                                                                                                                            acCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCa       >  2:2971910/1‑151 (MQ=255)
                                                                                                                                             cccTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAg      <  1:11529680/151‑1 (MQ=255)
                                                                                                                                               cTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCg    <  1:4071478/151‑1 (MQ=255)
                                                                                                                                                 gaagaaGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGAg  >  1:1293447/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAAAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGAG  >  NC_008463/5530167‑5530462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑