Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,809,365 T→C K239E (AAG→GAG)  rlmB ← 23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,809,3650TC99.8% 2018.7 / NA 525K239E (AAG→GAG) rlmB23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (310/214);  minor base G (1/0);  total (311/214)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 529 total aligned reads displayed.

GCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCGT  >  NC_008463/5809218‑5809512
                                                                                                                                                   |                                                                                                                                                   
gcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAAc                                                                                                                                                 <  1:3305316‑M2/70‑1 (MQ=255)
gcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAAc                                                                                                                                                 >  1:6527849‑M2/82‑151 (MQ=255)
     aaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCa                                                                                                                                            >  1:2288255‑M2/77‑151 (MQ=255)
      aggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAg                                                                                                                                           >  2:8215122‑M2/76‑151 (MQ=255)
        gggcgacattctagagaagggcatccggcgaagcaaggaagattggcgaatatttgtaccgcggatggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGAc                                                                                                                                         <  2:4309913‑M2/78‑1 (MQ=255)
           cgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGcc                                                                                                                                      <  2:3335963‑M2/81‑1 (MQ=255)
               attctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTg                                                                                                                                  <  2:363249‑M2/85‑1 (MQ=255)
               attctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTg                                                                                                                                  >  1:11291197‑M2/67‑151 (MQ=255)
                   tagcgacgggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGa                                                                                                                              <  1:6094813‑M2/89‑1 (MQ=255)
                    agagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                                                                             >  1:9614894‑M2/62‑151 (MQ=255)
                          gggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGAc                                                                                                                       >  2:5485951‑M2/56‑151 (MQ=255)
                           taCCGGCGAAGCAAGGTAGATTGGCGAATGTTTGTCCAGCGGATGGAATG‑‑‑GCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGCTGTCGAACAGGCAGTCGGCGGTGGCGGCCGCGACGtgc                                                                                                                   >  2:9138881/2‑149 (MQ=255)
                           ggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACg                                                                                                                      <  2:2635673‑M2/97‑1 (MQ=255)
                             taaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCGCACTCCAGCGGGGTGCGCTGGCGGAGGCTCTCGAACAGGCAGACGCCGGTGGCGACCGAGacggt                                                                                                                    >  1:4969863‑M2/53‑149 (MQ=255)
                              aaccggcgaagcaaggaagattggcgaatatttgtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTc                                                                                                                   <  1:9922886‑M2/100‑1 (MQ=255)
                                 cggcgaagcaaggaagattggcgaatatttgtacagcggatggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCCCGCGCACACTCCAGCGGGGTGCGCTGGAGGCGGATCTCGAACATGCAGACGCCGGTGGCGACCGAGACGTTCAgg                                                                                                                >  1:4424760‑M2/49‑151 (MQ=255)
                                 cggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAgg                                                                                                                <  2:6583170‑M2/103‑1 (MQ=255)
                                  ggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgc                                                                                                               >  1:3517032‑M2/48‑151 (MQ=255)
                                  ggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgc                                                                                                               <  2:7961100‑M2/104‑1 (MQ=255)
                                   gcgaagcaagtaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgct                                                                                                              <  1:8828224‑M2/105‑1 (MQ=255)
                                    cgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctg                                                                                                             <  1:2180666‑M2/106‑1 (MQ=255)
                                       agcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGctgctg                                                                                                          <  2:2084258‑M2/109‑1 (MQ=255)
                                       agcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGctgctg                                                                                                          >  2:5390583‑M2/43‑151 (MQ=255)
                                             gaagattggcgaatatgtgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTg                                                                                                    <  2:1203393‑M2/115‑1 (MQ=255)
                                              aagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGAGGCGACCGAGACGTTCAGGCTGCTGACGCTGc                                                                                                   >  2:7433548‑M2/36‑151 (MQ=255)
                                               agattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCCGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGcc                                                                                                  >  1:6399259‑M2/35‑151 (MQ=255)
                                                gattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCg                                                                                                 <  1:9747157‑M2/118‑1 (MQ=255)
                                                       gaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGTCGTTCAGGCTGCTGACGCTGCCGGCCATCg                                                                                          >  2:10952124‑M2/27‑151 (MQ=255)
                                                       gaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGTGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCg                                                                                          >  1:165914‑M2/27‑151 (MQ=255)
                                                        aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGTTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGGCGGTGGCGGCCAGCgg                                                                                         >  1:7482661‑M2/26‑151 (MQ=255)
                                                        aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCgg                                                                                         <  1:4775481‑M2/126‑1 (MQ=255)
                                                        aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCgg                                                                                         <  1:11692000‑M2/126‑1 (MQ=255)
                                                        aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCgg                                                                                         <  1:10120632‑M2/126‑1 (MQ=255)
                                                         tttGTTCAGCGGATGGTCTG‑‑‑GCGCGGGG‑CCGCGCCGGGGCGGCGCCGCACGCGGTGACTCCAGCGGGGTGCGCTGGCGGTGGATCGGGAAGTGGGCGTCGCCGGTGGCGGCCGTGTCGTTCAGGGTGCGGTCGGTGCCGGGCAGCGGCacc                                                                                     >  2:10116828/1‑149 (MQ=255)
                                                         atatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGc                                                                                        >  2:5129731‑M2/25‑151 (MQ=255)
                                                           atttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTAAGGCTGCTGACGCTGCCGGCCATCGGCaa                                                                                      >  1:8313944‑M2/23‑151 (MQ=255)
                                                             ttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAAcc                                                                                    >  2:7711964‑M2/21‑151 (MQ=255)
                                                             tACAGCGGATGGAATG‑‑‑GCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCCGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCCGACGCCGGTGGCGACCGAGACGTTCAGGCAGCTGACGCGGCCGGCCATCGGCAACCTgg                                                                                 >  2:3003840/1‑151 (MQ=255)
                                                              tgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAAAATGCAGACGCCCGTGGCGAACGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAacca                                                                                   >  1:10880807‑M2/20‑150 (MQ=255)
                                                                tacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTgg                                                                                 <  1:4148226‑M2/134‑1 (MQ=255)
                                                                  cagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCg                                                                               <  2:3219957‑M2/136‑1 (MQ=255)
                                                                           gaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCAGGCGGAGGATCTCGAACAGGCAGACGCCGGGGGCGACCGAGACGTTCAGGCTGAGGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCg                                                                      >  1:7066495‑M2/7‑151 (MQ=255)
                                                                            aatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGc                                                                     <  2:9214745‑M2/146‑1 (MQ=255)
                                                                              tggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAg                                                                   >  2:1653903‑M2/4‑151 (MQ=255)
                                                                               ggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGt                                                                  >  2:8314168‑M2/3‑151 (MQ=255)
                                                                               ggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGt                                                                  <  1:7279559‑M2/149‑1 (MQ=255)
                                                                                  gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCt                                                               <  1:6882971/151‑1 (MQ=255)
                                                                                         ccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCggg                                                         <  2:1031668/146‑1 (MQ=255)
                                                                                         ccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCggg                                                         <  2:11037775/146‑1 (MQ=255)
                                                                                          cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGt                                                        <  2:6693798/147‑1 (MQ=255)
                                                                                              ccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGc                                                    >  2:6218504/1‑151 (MQ=255)
                                                                                                 cggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCgg                                                 <  2:4630841/147‑1 (MQ=255)
                                                                                                    cggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGgcgc                                              >  1:5600130/2‑151 (MQ=255)
                                                                                                      gCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCAt                                            <  1:2974557/151‑1 (MQ=255)
                                                                                                      gCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCAt                                            <  2:3852508/151‑1 (MQ=255)
                                                                                                       ccccGCACGCGCTCACTCCAGCGGGGTACGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATg                                           >  2:2763215/1‑151 (MQ=255)
                                                                                                        cccGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGTCGGAGGCCTGCGGGGTGGTGAGGCTGCCGGCCATCGGCGGCCTGTGGGGGAAGTCGCGGTGCTCGCGCGTCGGGCGGCGCATGc                                          >  2:6823075/1‑151 (MQ=255)
                                                                                                        cccGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGc                                          <  1:11038329/151‑1 (MQ=255)
                                                                                                        cccGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGc                                          >  2:1981944/1‑151 (MQ=255)
                                                                                                          cGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGACGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGccc                                        >  2:9592673/1‑151 (MQ=255)
                                                                                                          cGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGccc                                        >  1:6699596/1‑151 (MQ=255)
                                                                                                          cGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGccc                                        >  2:9068035/1‑151 (MQ=255)
                                                                                                            cACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCACCCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCtg                                      >  1:1188849/1‑150 (MQ=255)
                                                                                                            cACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCtt                                      >  2:10400138/1‑151 (MQ=255)
                                                                                                             aCGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTg                                     >  1:8621449/1‑151 (MQ=255)
                                                                                                             aCGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTg                                     >  2:107677/1‑151 (MQ=255)
                                                                                                             aCGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTg                                     <  1:1337239/151‑1 (MQ=255)
                                                                                                              cgcgCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGc                                    >  2:4064996/1‑151 (MQ=255)
                                                                                                               gcgcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGcc                                   >  1:3815094/1‑151 (MQ=255)
                                                                                                               gcgcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGcc                                   >  2:1304701/1‑151 (MQ=255)
                                                                                                                cgcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCt                                  <  1:3108691/151‑1 (MQ=255)
                                                                                                                cgcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCt                                  >  1:8995190/1‑151 (MQ=255)
                                                                                                                cgcTCACACAAGCGTCGAGCGCTGGCTGAGGATCTCGAACAGGCAGACGCCGGAGGCGACAGAGACCTACATGCTCCAGACGCAGCCGGCCAACGGCAACCAGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCt                                  >  2:4738406/1‑151 (MQ=255)
                                                                                                                 gcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCACCCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCtt                                 >  2:10511418/1‑151 (MQ=255)
                                                                                                                 gcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCtt                                 >  2:5552822/1‑151 (MQ=255)
                                                                                                                 gcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCtt                                 <  2:11094076/151‑1 (MQ=255)
                                                                                                                   tCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTcc                               >  1:9202490/1‑151 (MQ=255)
                                                                                                                    cACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcg                              <  2:1849916/151‑1 (MQ=255)
                                                                                                                    cACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcg                              >  2:8869134/1‑151 (MQ=255)
                                                                                                                        ccAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGcc                          >  2:56803/1‑151 (MQ=255)
                                                                                                                         cAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGccc                         >  1:11523619/1‑151 (MQ=255)
                                                                                                                         cAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGccc                         >  1:6142551/1‑151 (MQ=255)
                                                                                                                          aGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCTGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTGCCGCGcccg                        >  1:5729831/1‑150 (MQ=255)
                                                                                                                          aGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCa                        <  1:1061050/151‑1 (MQ=255)
                                                                                                                            cGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATc                      <  1:7608019/151‑1 (MQ=255)
                                                                                                                             ggggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCa                     >  1:1024765/1‑151 (MQ=255)
                                                                                                                              gggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCAc                    >  2:6470411/1‑151 (MQ=255)
                                                                                                                               ggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCAcc                   >  2:8546127/1‑151 (MQ=255)
                                                                                                                                  gcgcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGc                >  2:4262999/1‑151 (MQ=255)
                                                                                                                                    gcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCAc              >  1:5259785/1‑151 (MQ=255)
                                                                                                                                    gcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCAc              <  2:6096203/151‑1 (MQ=255)
                                                                                                                                     cTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACg             >  2:11670524/1‑151 (MQ=255)
                                                                                                                                        gCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTc          <  2:9414215/151‑1 (MQ=255)
                                                                                                                                         cGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCg         <  2:7278237/151‑1 (MQ=255)
                                                                                                                                         cGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCg         >  1:3160276/1‑151 (MQ=255)
                                                                                                                                          ggaggaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCgg        >  2:11263202/1‑151 (MQ=255)
                                                                                                                                           gaggaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGc       <  2:6921460/151‑1 (MQ=255)
                                                                                                                                           gaggaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGc       >  1:10525329/1‑151 (MQ=255)
                                                                                                                                            aggaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGcc      >  1:2844612/1‑151 (MQ=255)
                                                                                                                                                tctcGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCGt  >  1:7947086/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCGT  >  NC_008463/5809218‑5809512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑