Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 982,940 A→G F293F (TTT→TTC)  PA14_RS04570 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463982,9400AG99.8% 2237.5 / NA 561F293F (TTT→TTC) PA14_RS04570ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (252/308);  minor base T (0/1);  total (252/309)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 564 total aligned reads displayed.

CCTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACAAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAGG  >  NC_008463/982792‑983087
                                                                                                                                                    |                                                                                                                                                   
    ggCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGt                                                                                                                                               <  1:6438607/151‑1 (MQ=255)
     gCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTg                                                                                                                                              <  1:7726250/151‑1 (MQ=255)
     gCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTg                                                                                                                                              <  1:8236073/151‑1 (MQ=255)
      ccAGCAGCAGGCCGAAGCTGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGAATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGc                                                                                                                                             <  2:11389048/151‑1 (MQ=255)
        agcagcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCt                                                                                                                                           <  2:5874192/151‑1 (MQ=255)
           agcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtct                                                                                                                                        <  2:7603849/151‑1 (MQ=255)
            gcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtctc                                                                                                                                       <  1:1678109/151‑1 (MQ=255)
             cagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGTGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcg                                                                                                                                      >  1:4597697/1‑151 (MQ=255)
             cagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcg                                                                                                                                      >  1:6089536/1‑151 (MQ=255)
              atgcctcaGATGTGCCAGGGGCGCTCGCCCAGCGAGAGCAGGATGGCCATCTCCCGGGGGAGCTAGTTGAGGCTGGTGAGGATCGCCGTGAGGATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcgg                                                                                                                                     <  1:1257206/144‑1 (MQ=255)
                 ccGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcggcgg                                                                                                                                  <  2:2193566/151‑1 (MQ=255)
                 ccGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcggcgg                                                                                                                                  >  2:11616354/1‑151 (MQ=255)
                    aaGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGc                                                                                                                               >  2:5208927/1‑151 (MQ=255)
                    aaGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGc                                                                                                                               <  2:2518696/151‑1 (MQ=255)
                      gATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGccc                                                                                                                             >  2:4950679/1‑151 (MQ=255)
                          ttccAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGATGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGGGAGACCACGTACATTGCCTTCTCGGCGGTGCCCATct                                                                                                                         <  2:2367951/149‑2 (MQ=255)
                          tgCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACTAACAGTGCCTTCTCGGCGGTGCCCATCa                                                                                                                         <  1:6377296/151‑1 (MQ=255)
                           gCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAg                                                                                                                        <  2:6952322/151‑1 (MQ=255)
                            ccAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAgg                                                                                                                       >  2:9323386/1‑151 (MQ=255)
                              aGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGcc                                                                                                                     >  1:2255398/1‑151 (MQ=255)
                              aGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGcc                                                                                                                     >  1:11001151/1‑151 (MQ=255)
                                  gcgcgcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCcac                                                                                                                 <  2:3410550/151‑1 (MQ=255)
                                     cgcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGt                                                                                                              >  2:8210947/1‑151 (MQ=255)
                                      gcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGtt                                                                                                             >  2:9761981/1‑151 (MQ=255)
                                      gcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGtt                                                                                                             >  1:8143566/1‑151 (MQ=255)
                                      gcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGtt                                                                                                             >  1:4200302/1‑151 (MQ=255)
                                       cgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTc                                                                                                            >  1:10548805/1‑151 (MQ=255)
                                        gcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTcc                                                                                                           >  2:3676386/1‑151 (MQ=255)
                                           cACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGc                                                                                                        >  2:4248570/1‑151 (MQ=255)
                                            aCCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCa                                                                                                       <  1:8359281/151‑1 (MQ=255)
                                            aCCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCa                                                                                                       <  2:1696907/151‑1 (MQ=255)
                                             ccGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAg                                                                                                      <  2:4430505/151‑1 (MQ=255)
                                              cGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAgg                                                                                                     <  1:380388/151‑1 (MQ=255)
                                                aGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGc                                                                                                   <  1:6562752/151‑1 (MQ=255)
                                                  cgcAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGa                                                                                                 <  2:10002842/151‑1 (MQ=255)
                                                   gcAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGac                                                                                                <  1:4359983/151‑1 (MQ=255)
                                                        aTGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCgg                                                                                           >  1:534021/1‑151 (MQ=255)
                                                                ctcCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATc                                                                                   >  1:1885710/1‑151 (MQ=255)
                                                                ctcCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATc                                                                                   >  1:5764099/1‑151 (MQ=255)
                                                                 tcCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCg                                                                                  <  2:8484450/151‑1 (MQ=255)
                                                                 tcCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCg                                                                                  >  1:2407025/1‑151 (MQ=255)
                                                                  cccGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGc                                                                                 <  2:8377687/151‑1 (MQ=255)
                                                                     ggcggcGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCAt                                                                              <  2:2803883/151‑1 (MQ=255)
                                                                       cggcgCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCa                                                                            <  1:10316232/151‑1 (MQ=255)
                                                                       cggcgCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCa                                                                            <  2:11050166/151‑1 (MQ=255)
                                                                         gcgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGc                                                                          <  1:4862141/151‑1 (MQ=255)
                                                                         gcgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGc                                                                          >  2:912954/1‑151 (MQ=255)
                                                                         gcgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGc                                                                          <  2:10731800/151‑1 (MQ=255)
                                                                         gcgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGc                                                                          <  2:4839626/151‑1 (MQ=255)
                                                                         gcgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGAGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGc                                                                          <  1:554249/151‑1 (MQ=255)
                                                                          cgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCg                                                                         >  1:2770699/1‑151 (MQ=255)
                                                                            cTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGt                                                                       <  1:8698475/151‑1 (MQ=255)
                                                                            cTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGt                                                                       >  2:4620321/1‑151 (MQ=255)
                                                                              cGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTc                                                                     <  1:9731314/151‑1 (MQ=255)
                                                                               gTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCg                                                                    >  1:887049/1‑151 (MQ=255)
                                                                                ttGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGc                                                                   <  1:261774/151‑1 (MQ=255)
                                                                                      cTGGTGAGGATCGCCGTGAGCATGCCGCTCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCGTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGAt                                                             <  1:8516238/151‑1 (MQ=255)
                                                                                      cTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGAt                                                             <  2:5645229/151‑1 (MQ=255)
                                                                                       tGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATa                                                            >  2:7080665/1‑151 (MQ=255)
                                                                                       tGGAGAGGATCGCCGCGAGCATGCCGATCCGGCCGGTGAGGACCACGAACAGCGAGACCACGAACCGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATa                                                            <  2:402173/151‑1 (MQ=255)
                                                                                          tGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTc                                                         <  1:9277815/151‑1 (MQ=255)
                                                                                          tGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTc                                                         >  1:11693743/1‑151 (MQ=255)
                                                                                            aGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGt                                                       <  1:3393486/151‑1 (MQ=255)
                                                                                             ggATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGtt                                                      <  2:3325866/151‑1 (MQ=255)
                                                                                               ctCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGa                                                    <  2:7376837/150‑1 (MQ=255)
                                                                                                  gCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCt                                                 <  1:1641670/151‑1 (MQ=255)
                                                                                                  gCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCt                                                 >  1:954028/1‑151 (MQ=255)
                                                                                                   ccGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTc                                                <  2:6072/151‑1 (MQ=255)
                                                                                                      tGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTcgcg                                             <  1:3910661/151‑1 (MQ=255)
                                                                                                       gAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGt                                            >  2:5389981/1‑151 (MQ=255)
                                                                                                        aGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGtt                                           <  2:8605199/151‑1 (MQ=255)
                                                                                                            tGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAg                                       <  2:10311738/151‑1 (MQ=255)
                                                                                                             gCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAgg                                      <  2:3098309/151‑1 (MQ=255)
                                                                                                             gCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAgg                                      >  1:6825599/1‑151 (MQ=255)
                                                                                                               cGATCAGGCCGGTGAGGACGACGATCAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGc                                    <  2:7744099/151‑1 (MQ=255)
                                                                                                               cGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGc                                    >  1:356623/1‑151 (MQ=255)
                                                                                                               cGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGc                                    >  1:11282301/1‑151 (MQ=255)
                                                                                                                gATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCg                                   >  2:1299349/1‑151 (MQ=255)
                                                                                                                gATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGGTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCg                                   <  2:6393067/151‑1 (MQ=255)
                                                                                                                 aTCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGa                                  <  2:1419597/151‑1 (MQ=255)
                                                                                                                 aTCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGa                                  >  1:1290797/1‑151 (MQ=255)
                                                                                                                   cAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAg                                <  2:106009/151‑1 (MQ=255)
                                                                                                                   cAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAg                                >  1:11563984/1‑151 (MQ=255)
                                                                                                                    aGGCCGGTGAGGACGACGAACAGCGAGACCACGAAATGTGCCTTCTCGGCGGTTCCCATCAGGCCCCACTGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATATTCGTTTACCTCGCGTTGCAGGGCGAAgg                               <  2:11726506/151‑1 (MQ=255)
                                                                                                                     ggCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGt                              <  2:6276482/151‑1 (MQ=255)
                                                                                                                       ccGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCg                            >  2:887826/1‑151 (MQ=255)
                                                                                                                          gTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCa                         >  2:8097290/1‑151 (MQ=255)
                                                                                                                            gAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCAcc                       >  2:352386/1‑151 (MQ=255)
                                                                                                                             aGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCt                      <  2:7287244/151‑1 (MQ=255)
                                                                                                                              ggACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGGGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCtt                     <  1:6632614/151‑1 (MQ=255)
                                                                                                                                 cgacgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGct                  >  2:1914032/1‑151 (MQ=255)
                                                                                                                                  gacgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGctc                 >  2:5243689/1‑151 (MQ=255)
                                                                                                                                    cgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCtt               <  1:7920145/151‑1 (MQ=255)
                                                                                                                                    cgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCtt               <  1:1078623/151‑1 (MQ=255)
                                                                                                                                     gaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTc              >  1:7528350/1‑151 (MQ=255)
                                                                                                                                     gaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTc              >  2:6636235/1‑151 (MQ=255)
                                                                                                                                       aCAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAg            <  1:10023853/151‑1 (MQ=255)
                                                                                                                                               aCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCa    >  2:5556926/1‑151 (MQ=255)
                                                                                                                                               aCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCa    <  1:3111332/151‑1 (MQ=255)
                                                                                                                                               aCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCa    <  1:912954/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACAAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAGG  >  NC_008463/982792‑983087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑