Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,027,111 A→C T236P (ACC→CCC)  mpl → UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,027,1110AC100.0% 1987.5 / NA 501T236P (ACC→CCC) mplUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (260/241);  total (260/241)

Only 100 of 506 total aligned reads displayed.

GGAATTCGACCACGCGGATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGACCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGCACAACGTCGCCAACGCCC  >  NC_008463/1026963‑1027258
                                                                                                                                                    |                                                                                                                                                   
 gAATTCGACCACGCGGATATCGTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGcccc                                                                                                                                                  <  1:3852435/151‑1 (MQ=255)
    ttCGACCACGCGGATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCgg                                                                                                                                               >  2:2501751/1‑151 (MQ=255)
    ttCGACCACGCGGATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCgg                                                                                                                                               >  2:4998936/1‑151 (MQ=255)
       gACCACGCGGATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGc                                                                                                                                            >  1:5411959/1‑151 (MQ=255)
        aCCACGCGGATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCa                                                                                                                                           <  1:302501/151‑1 (MQ=255)
         ccACGCGGATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAg                                                                                                                                          >  2:4665597/1‑151 (MQ=255)
          cACGCGGATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGa                                                                                                                                         <  2:5998004/151‑1 (MQ=255)
           aCGCGGATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGac                                                                                                                                        >  2:2254672/1‑151 (MQ=255)
             gcgGATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGacca                                                                                                                                      <  1:84162/151‑1 (MQ=255)
              cgGATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGaccac                                                                                                                                     <  2:4978414/151‑1 (MQ=255)
               ggATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGaccacc                                                                                                                                    <  1:5824624/151‑1 (MQ=255)
               ggATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGaccacc                                                                                                                                    <  1:4323168/151‑1 (MQ=255)
                gATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCg                                                                                                                                   >  1:10942946/1‑151 (MQ=255)
                gATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCg                                                                                                                                   <  1:2257426/151‑1 (MQ=255)
                 atatCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCgg                                                                                                                                  <  1:10053308/151‑1 (MQ=255)
                 atatCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCgg                                                                                                                                  >  2:3350966/1‑151 (MQ=255)
                   atCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCg                                                                                                                                >  1:871562/1‑151 (MQ=255)
                       tCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGGGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAgg                                                                                                                            <  2:11204283/151‑1 (MQ=255)
                           cGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGCGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAggcggc                                                                                                                        <  2:686632/151‑1 (MQ=255)
                           cGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAggcggc                                                                                                                        >  2:9627773/1‑151 (MQ=255)
                            gACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCAGTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGcc                                                                                                                       <  2:10728289/151‑1 (MQ=255)
                             aCCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCa                                                                                                                      >  2:7644044/1‑151 (MQ=255)
                              ccTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAg                                                                                                                     >  2:4208426/1‑151 (MQ=255)
                                tCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTg                                                                                                                   <  1:4501243/151‑1 (MQ=255)
                                  gcgGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGc                                                                                                                 <  1:261855/151‑1 (MQ=255)
                                  gcgGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGc                                                                                                                 <  2:10883731/151‑1 (MQ=255)
                                    ggCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAg                                                                                                               >  1:7498283/1‑151 (MQ=255)
                                     gCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAgg                                                                                                              <  1:9408458/151‑1 (MQ=255)
                                      ccATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGgc                                                                                                             <  1:9047917/151‑1 (MQ=255)
                                       cATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGgcg                                                                                                            >  1:1278215/1‑151 (MQ=255)
                                       cATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGgcg                                                                                                            >  1:1365454/1‑151 (MQ=255)
                                        aTCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGgcgc                                                                                                           >  2:9081360/1‑151 (MQ=255)
                                        aTCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGgcgc                                                                                                           >  1:3565010/1‑151 (MQ=255)
                                        aTCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGgcgc                                                                                                           <  2:7515026/151‑1 (MQ=255)
                                           gAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGcc                                                                                                        >  2:8540724/1‑151 (MQ=255)
                                              cGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGc                                                                                                     <  2:2600704/151‑1 (MQ=255)
                                                gCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGcac                                                                                                   <  1:1587696/151‑3 (MQ=255)
                                                 cAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCg                                                                                                  >  2:1899887/1‑151 (MQ=255)
                                                   gTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGcc                                                                                                <  2:4778468/151‑1 (MQ=255)
                                                        accaTCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGa                                                                                           >  2:2448526/1‑151 (MQ=255)
                                                        accaTCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGa                                                                                           >  2:2796739/1‑151 (MQ=255)
                                                            tCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGc                                                                                       <  1:2957465/151‑1 (MQ=255)
                                                               ggTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTcg                                                                                    <  2:3843104/151‑1 (MQ=255)
                                                                   cgcACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCt                                                                                <  2:9428058/151‑1 (MQ=255)
                                                                    gcACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCtt                                                                               <  2:7443722/151‑1 (MQ=255)
                                                                     cACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTc                                                                              <  2:397755/151‑1 (MQ=255)
                                                                        cGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAg                                                                           >  2:8178988/1‑151 (MQ=255)
                                                                         gTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGTCAGCCCTGGAGCGCGTACTCGGGATGGGCGGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCTGGCGCGCCTGCTCGCCGAGGACGGCGCGCGCTTCGAgg                                                                          >  2:6200795/1‑151 (MQ=255)
                                                                         gTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAgg                                                                          <  2:2681809/151‑1 (MQ=255)
                                                                          tGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGt                                                                         <  1:6360894/151‑1 (MQ=255)
                                                                          tGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGt                                                                         <  2:3087414/151‑1 (MQ=255)
                                                                              gggCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGCGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCTCCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGcgg                                                                     >  2:7177024/1‑149 (MQ=255)
                                                                              gggCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTg                                                                     <  2:4576149/151‑1 (MQ=255)
                                                                              gggCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTg                                                                     >  1:4221849/1‑151 (MQ=255)
                                                                               ggCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGt                                                                    >  2:1013212/1‑151 (MQ=255)
                                                                                gCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGtt                                                                   >  1:6787823/1‑151 (MQ=255)
                                                                                 cGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTc                                                                  <  2:8727186/151‑1 (MQ=255)
                                                                                  gAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCg                                                                 >  2:3450226/1‑151 (MQ=255)
                                                                                    gggCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGAc                                                               >  2:10603464/1‑151 (MQ=255)
                                                                                      gCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGGTCGACgg                                                             >  2:1548485/1‑151 (MQ=255)
                                                                                        cTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCa                                                           <  2:11583099/151‑1 (MQ=255)
                                                                                        cTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCa                                                           >  1:5126554/1‑151 (MQ=255)
                                                                                          gATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATc                                                         <  2:1687888/151‑1 (MQ=255)
                                                                                           aTCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCg                                                        <  1:5654982/151‑1 (MQ=255)
                                                                                             cATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTc                                                      <  1:3906767/151‑1 (MQ=255)
                                                                                                  gCCCGAGCGCCGAGACAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAggg                                                 <  2:5096156/151‑1 (MQ=255)
                                                                                                  gCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAggg                                                 <  1:10343065/151‑1 (MQ=255)
                                                                                                   cccGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGTGGTGCTGTTCGACGGCATCGTCCAGGGc                                                <  2:8074031/151‑1 (MQ=255)
                                                                                                   cccGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGc                                                >  1:7909711/1‑151 (MQ=255)
                                                                                                    ccGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCg                                               <  1:4954427/151‑1 (MQ=255)
                                                                                                         cgcCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCTAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTg                                          >  2:2975649/1‑151 (MQ=255)
                                                                                                         cgcCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTg                                          >  2:5308266/1‑151 (MQ=255)
                                                                                                          gcCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTgg                                         >  1:11519293/1‑151 (MQ=255)
                                                                                                          gcCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTgg                                         <  1:2032003/151‑1 (MQ=255)
                                                                                                          gcCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTgg                                         >  2:5640231/1‑151 (MQ=255)
                                                                                                           ccGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGa                                        >  2:9776449/1‑151 (MQ=255)
                                                                                                           ccGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGa                                        <  1:10562138/151‑1 (MQ=255)
                                                                                                              agaAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTg                                     >  2:6415637/1‑151 (MQ=255)
                                                                                                                aaaGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGc                                   >  1:9941348/1‑151 (MQ=255)
                                                                                                                aaaGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGc                                   >  2:7034193/1‑151 (MQ=255)
                                                                                                                 aaGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGcc                                  <  1:2752729/151‑1 (MQ=255)
                                                                                                                   gCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGc                                <  2:10497991/151‑1 (MQ=255)
                                                                                                                     ccTGGAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTg                              >  1:8967914/1‑151 (MQ=255)
                                                                                                                        ggAGCGCGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGAcc                           <  2:3241432/151‑1 (MQ=255)
                                                                                                                           gcgcGTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGc                        <  2:11480743/151‑1 (MQ=255)
                                                                                                                            cgcgTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGcc                       >  1:6119419/1‑151 (MQ=255)
                                                                                                                               gTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGc                    >  1:6990778/1‑151 (MQ=255)
                                                                                                                               gTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGc                    >  1:9651731/1‑151 (MQ=255)
                                                                                                                               gTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGc                    <  2:8390977/151‑1 (MQ=255)
                                                                                                                               gTACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGc                    <  1:8031227/151‑1 (MQ=255)
                                                                                                                                tACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCTAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGca                   <  1:1830087/151‑1 (MQ=255)
                                                                                                                                tACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGca                   >  2:6578766/1‑151 (MQ=255)
                                                                                                                                tACTCGGGATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGca                   <  2:2107675/151‑1 (MQ=255)
                                                                                                                                     gggATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGCACAACg              >  1:11191941/1‑151 (MQ=255)
                                                                                                                                      ggATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGCACAACGt             >  1:7247744/1‑151 (MQ=255)
                                                                                                                                       gATGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGCACAACGTc            >  2:4389836/1‑151 (MQ=255)
                                                                                                                                         tGGGCTGCTGGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGCACAACGTCGc          <  2:1033325/151‑1 (MQ=255)
                                                                                                                                              tgctgGCCCCCGGTGCAGACCACCGGCGAGGGCGGCGAGGGGCAGGCGCGCCTGCGCGCCGAGGACGGCTCGCGCGTCGAGGTGCTGGTCGTCGGCAGCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGCACTACGTCGCCAACg     >  2:290000/1‑151 (MQ=255)
                                                                                                                                                ctgGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGCACAACGTCGCCAACGcc   <  1:11763113/151‑1 (MQ=255)
                                                                                                                                                 tgGCCCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGCACAACGTCGCCAACGccc  >  1:6622514/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGAATTCGACCACGCGGATATCTTCCCCGACCTCGCGGCCATCGAGCGGCAGTTCCACCATCTGGTGCGCACCGTGCCGGGCGAGGGCCTGATCATTCGCCCGAGCGCCGAGAAAGCCCTGGAGCGCGTACTCGGGATGGGCTGCTGGACCCCGGTGCAGACCACCGGCGAAGGCGGCCAGTGGCAGGCGCGCCTGCTCGCCGAGGACGGCTCGCGCTTCGAGGTGCTGTTCGACGGCATCGTCCAGGGCGAGGTGGACTGGCCGCTGACCGGCCTGCACAACGTCGCCAACGCCC  >  NC_008463/1026963‑1027258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑