Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,375,947 A→G intergenic (+18/‑38) PA14_RS06470 → / → PA14_RS06475 PspA/IM30 family protein/DUF1449 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,375,9470AG99.8% 1955.8 / NA 486intergenic (+18/‑38)PA14_RS06470/PA14_RS06475PspA/IM30 family protein/DUF1449 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (249/236);  minor base C (1/0);  total (250/236)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 492 total aligned reads displayed.

CGGCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCAAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCGAGAT  >  NC_008463/1375799‑1376094
                                                                                                                                                    |                                                                                                                                                   
cgGCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCgaa                                                                                                                                                   <  2:3989494/151‑1 (MQ=255)
cgGCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCgaa                                                                                                                                                   <  1:1586094/151‑1 (MQ=255)
 ggCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAg                                                                                                                                                  <  1:10327531/151‑1 (MQ=255)
 ggCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAg                                                                                                                                                  >  1:3999021/1‑151 (MQ=255)
      aaCTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTc                                                                                                                                             <  1:8900752/151‑1 (MQ=255)
         tGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCgg                                                                                                                                          <  1:3344444/151‑1 (MQ=255)
          ggCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCggg                                                                                                                                         >  2:4283318/1‑151 (MQ=255)
            ccAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACCGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGcc                                                                                                                                       >  2:9181788/1‑151 (MQ=255)
              aGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCGTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGGCCGGGCCCg                                                                                                                                     >  1:6605885/1‑151 (MQ=255)
              aGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCg                                                                                                                                     >  1:10711415/1‑151 (MQ=255)
               gcgcGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGt                                                                                                                                    >  1:3461387/1‑151 (MQ=255)
               gcgcGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGt                                                                                                                                    >  2:2341945/1‑151 (MQ=255)
                cgcgGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGtt                                                                                                                                   <  1:7721757/151‑1 (MQ=255)
                   ggcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGACCAGGGCAAGGCGTGATAGGCCCGCGGCCCGGCGATGGCGTGCGGGCCCGttttt                                                                                                                                >  1:2710008/1‑151 (MQ=255)
                     cggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCCACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCa                                                                                                                              >  2:7617472/1‑151 (MQ=255)
                     cggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCa                                                                                                                              >  1:8335398/1‑151 (MQ=255)
                     cggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCa                                                                                                                              <  1:9359844/151‑1 (MQ=255)
                      ggcgCCGGACGATGCGCTGGACGCCACGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCACCAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTctg                                                                                                                             >  1:525582/1‑149 (MQ=255)
                      ggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAATGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTctg                                                                                                                             >  1:5463336/1‑149 (MQ=255)
                        cgcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCTAGGCCTGTTAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAggg                                                                                                                           <  2:4792463/151‑1 (MQ=255)
                        cgcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAggg                                                                                                                           <  1:8144808/151‑1 (MQ=255)
                         gcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGa                                                                                                                          >  1:6064358/1‑151 (MQ=255)
                            ggACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGGGGATGGCGTGCTGGCGCGCCTGAAGGTCAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGGCCGGGCCCGTTTTTCTGGGATag                                                                                                                       >  1:4693199/1‑151 (MQ=255)
                              aCGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATagag                                                                                                                     >  1:9013725/1‑151 (MQ=255)
                               cGATGCGCGGGACGCCACGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGc                                                                                                                    <  2:11078707/151‑1 (MQ=255)
                                    cgcTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACaa                                                                                                               >  1:4319220/1‑151 (MQ=255)
                                      cTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATg                                                                                                             >  1:6972226/1‑151 (MQ=255)
                                      cTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATg                                                                                                             <  2:7530628/151‑1 (MQ=255)
                                        ggACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGa                                                                                                           <  2:7809711/151‑1 (MQ=255)
                                         gACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGaa                                                                                                          <  1:10136659/151‑1 (MQ=255)
                                          aCGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAg                                                                                                         <  1:1311813/151‑1 (MQ=255)
                                           cGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAgg                                                                                                        <  1:9787526/151‑1 (MQ=255)
                                           cGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAgg                                                                                                        >  2:8150266/1‑151 (MQ=255)
                                             ccAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATCGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGtt                                                                                                      >  2:580959/1‑151 (MQ=255)
                                              cAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGttt                                                                                                     <  1:3238172/151‑1 (MQ=255)
                                                aGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTcc                                                                                                   <  1:3148309/151‑1 (MQ=255)
                                                aGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTcc                                                                                                   <  2:2610512/151‑1 (MQ=255)
                                                   tGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGc                                                                                                >  2:5471396/1‑151 (MQ=255)
                                                   tGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGc                                                                                                >  2:4603406/1‑151 (MQ=255)
                                                    gcgcgcGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCa                                                                                               <  1:1838847/151‑1 (MQ=255)
                                                     cgcgcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAg                                                                                              <  1:4142776/151‑1 (MQ=255)
                                                     cgcgcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAg                                                                                              <  2:3445775/151‑1 (MQ=255)
                                                     cgcgcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAg                                                                                              <  2:7033486/151‑1 (MQ=255)
                                                              gggATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTg                                                                                     >  1:3831983/1‑151 (MQ=255)
                                                                gATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTc                                                                                   <  2:2694036/151‑1 (MQ=255)
                                                                gATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTc                                                                                   >  2:114745/1‑151 (MQ=255)
                                                                gATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTc                                                                                   <  1:1133217/151‑1 (MQ=255)
                                                                 aTCACGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCg                                                                                  <  2:9075930/151‑1 (MQ=255)
                                                                       gCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCg                                                                            <  2:368194/151‑1 (MQ=255)
                                                                        ccTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGACGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGa                                                                           >  2:6487965/1‑151 (MQ=255)
                                                                              gCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGc                                                                     >  2:8762910/1‑151 (MQ=255)
                                                                              gCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGc                                                                     >  1:2897773/1‑151 (MQ=255)
                                                                               cAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCt                                                                    <  2:6798411/151‑1 (MQ=255)
                                                                               cAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCt                                                                    <  2:10624834/151‑1 (MQ=255)
                                                                                aaCAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTg                                                                   <  1:4448948/151‑1 (MQ=255)
                                                                                  cAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGtt                                                                 >  1:9108245/1‑151 (MQ=255)
                                                                                   aGCGTGGATGGCGTGCTGGCGCGCCTGAAGGACAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTc                                                                >  2:123085/1‑151 (MQ=255)
                                                                                    gCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCa                                                               <  2:9588940/151‑1 (MQ=255)
                                                                                     cGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAg                                                              <  2:2823572/151‑1 (MQ=255)
                                                                                        ggATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCtt                                                           <  1:5862612/151‑1 (MQ=255)
                                                                                         gATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCttt                                                          >  1:10559692/1‑151 (MQ=255)
                                                                                           tGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTttct                                                        <  1:1081675/151‑1 (MQ=255)
                                                                                           tGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTttct                                                        <  1:7296003/151‑1 (MQ=255)
                                                                                             gCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTttcttc                                                      >  1:226683/1‑151 (MQ=255)
                                                                                              cGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTtcttct                                                     >  1:6760268/1‑151 (MQ=255)
                                                                                              cGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTtcttct                                                     >  1:10005490/1‑151 (MQ=255)
                                                                                              cGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTtcttct                                                     <  1:4196667/151‑1 (MQ=255)
                                                                                                   tGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGCGCCCGTTTTTCAGGGATAGAGCGACAATGGCAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATcc                                                >  1:2623224/1‑151 (MQ=255)
                                                                                                     gcgcgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTc                                              <  1:7412496/151‑1 (MQ=255)
                                                                                                      cgcgcCTGAACGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCCAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTcg                                             >  2:7210867/1‑151 (MQ=255)
                                                                                                           cTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatc                                        >  1:5127475/1‑151 (MQ=255)
                                                                                                            tGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACGATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTGCCGAGCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGagct                                       >  2:6171828/1‑148 (MQ=255)
                                                                                                            tGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatca                                       >  2:1894157/1‑151 (MQ=255)
                                                                                                             gAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatcat                                      >  1:230697/1‑151 (MQ=255)
                                                                                                               aGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCt                                    <  1:1217542/151‑1 (MQ=255)
                                                                                                               aGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCt                                    >  2:11227622/1‑151 (MQ=255)
                                                                                                                  aaaaGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACt                                 >  1:5774163/1‑151 (MQ=255)
                                                                                                                  aaaaGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACt                                 <  2:7976901/151‑1 (MQ=255)
                                                                                                                  aaaaGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACt                                 <  1:9502591/151‑1 (MQ=255)
                                                                                                                      gggCAAGGCCTGATAGGCCCGCGGCCCGGCGCAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTgggg                             >  2:6014503/1‑151 (MQ=255)
                                                                                                                      gggCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTgggg                             >  2:11703752/1‑151 (MQ=255)
                                                                                                                       ggCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGc                            >  2:2964328/1‑151 (MQ=255)
                                                                                                                        gCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCa                           >  2:8457617/1‑151 (MQ=255)
                                                                                                                         cAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAt                          >  1:11146511/1‑151 (MQ=255)
                                                                                                                          aaGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtc                         <  1:1951317/151‑1 (MQ=255)
                                                                                                                          aaGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtc                         <  1:4926912/151‑1 (MQ=255)
                                                                                                                          aaGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtc                         >  2:10321473/1‑151 (MQ=255)
                                                                                                                             gCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCAGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtcgtc                      >  1:1805579/1‑151 (MQ=255)
                                                                                                                               cTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCGACTGGGGCATCGTCGc                    >  2:1694062/1‑151 (MQ=255)
                                                                                                                                  aTAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCTGACGGCGCTGTCGTTTCCGACCGTGCGGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGTGGCATCGTCGCCCt                 >  2:2137747/1‑151 (MQ=255)
                                                                                                                                   tAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTg                >  1:11507780/1‑151 (MQ=255)
                                                                                                                                    aGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTgg               <  2:9873702/151‑1 (MQ=255)
                                                                                                                                        ccGCGGCCCGGCGACGGCGTCCGGGCCCGTTTTTCTGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCAt           >  1:8600645/1‑151 (MQ=255)
                                                                                                                                        ccGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCAt           >  1:10965554/1‑151 (MQ=255)
                                                                                                                                         cgcgGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATg          >  2:6656387/1‑151 (MQ=255)
                                                                                                                                           cgGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCTGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGt        >  1:7648665/1‑151 (MQ=255)
                                                                                                                                            ggCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTc       <  2:1480469/151‑1 (MQ=255)
                                                                                                                                            ggCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTc       >  1:8801327/1‑151 (MQ=255)
                                                                                                                                              cccGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCga     <  1:8430776/151‑1 (MQ=255)
                                                                                                                                                 ggCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCGAGAt  >  1:6234251/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCAAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCGAGAT  >  NC_008463/1375799‑1376094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑