Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,558,205 A→G Q390Q (CAA→CAG)  PA14_RS07290 → propionyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,558,2050AG100.0% 2016.6 / NA 502Q390Q (CAA→CAG) PA14_RS07290propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (242/260);  total (242/260)

Only 100 of 507 total aligned reads displayed.

GCAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAAACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAGGGC  >  NC_008463/1558057‑1558352
                                                                                                                                                    |                                                                                                                                                   
gcAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCAAAGTTCGATGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCGGGAAGAGAACAGCGGGCGGCCGGTCCGGGTCCATTGGTGGCAGAc                                                                                                                                                   >  2:11319459/1‑151 (MQ=255)
 cAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGAcc                                                                                                                                                  <  1:5357958/151‑1 (MQ=255)
  aaGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCg                                                                                                                                                 >  2:9837865/1‑151 (MQ=255)
  aaGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCg                                                                                                                                                 >  1:7158927/1‑151 (MQ=255)
  aaGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCg                                                                                                                                                 <  1:8018551/151‑1 (MQ=255)
    ggAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCgag                                                                                                                                               >  2:10848848/1‑151 (MQ=255)
      aaGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGAc                                                                                                                                             >  2:4170483/1‑151 (MQ=255)
       aGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGAcc                                                                                                                                            <  1:10007268/151‑1 (MQ=255)
       aGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGAcc                                                                                                                                            >  1:2767531/1‑151 (MQ=255)
           ccGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCt                                                                                                                                        <  2:8150805/151‑1 (MQ=255)
            cGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTg                                                                                                                                       <  1:4438031/151‑1 (MQ=255)
            cGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTg                                                                                                                                       >  2:4652463/1‑151 (MQ=255)
             gCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTgg                                                                                                                                      >  2:7919031/1‑151 (MQ=255)
              cATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGc                                                                                                                                     >  2:11638477/1‑151 (MQ=255)
               aTGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGcc                                                                                                                                    <  2:8470111/151‑1 (MQ=255)
               aTGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGcc                                                                                                                                    >  2:5051377/1‑151 (MQ=255)
               aTGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTCCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGcc                                                                                                                                    >  1:7811735/1‑151 (MQ=255)
                   cGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTg                                                                                                                                >  2:10974280/1‑151 (MQ=255)
                    gAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGa                                                                                                                               >  1:8730506/1‑151 (MQ=255)
                     aaCGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGAc                                                                                                                              >  1:10943536/1‑151 (MQ=255)
                     aaCGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGAc                                                                                                                              >  1:4128385/1‑151 (MQ=255)
                       cGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACcg                                                                                                                            <  2:10116208/151‑1 (MQ=255)
                       cGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACcg                                                                                                                            >  2:381748/1‑151 (MQ=255)
                         ggTGAAGCGCTACGACCTACGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACcgcg                                                                                                                          <  2:6553269/151‑1 (MQ=255)
                            gAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGccc                                                                                                                       >  1:523409/1‑151 (MQ=255)
                            gAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGccc                                                                                                                       >  2:4464355/1‑151 (MQ=255)
                             aaGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCt                                                                                                                      >  2:580329/1‑151 (MQ=255)
                             aaGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCt                                                                                                                      <  1:1427829/151‑1 (MQ=255)
                              aGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTg                                                                                                                     <  2:1875551/151‑1 (MQ=255)
                                cgcTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCa                                                                                                                   <  1:6396940/151‑1 (MQ=255)
                                  cTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATc                                                                                                                 >  1:364920/1‑151 (MQ=255)
                                   tACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCg                                                                                                                <  2:9565825/151‑1 (MQ=255)
                                     cGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCggg                                                                                                              >  1:5841429/1‑151 (MQ=255)
                                     cGACCTAAGGTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCggg                                                                                                              <  2:6062380/151‑1 (MQ=255)
                                       aCCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGAt                                                                                                            <  1:3004020/151‑1 (MQ=255)
                                        ccTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATc                                                                                                           <  2:2050231/151‑1 (MQ=255)
                                          tAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGc                                                                                                         <  2:7496215/151‑1 (MQ=255)
                                             gTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCgg                                                                                                      <  2:11461002/151‑1 (MQ=255)
                                                 gCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCAc                                                                                                  >  1:11230667/1‑151 (MQ=255)
                                                  cTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACg                                                                                                 >  2:1410776/1‑151 (MQ=255)
                                                    gcgcCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGAc                                                                                               >  2:271343/1‑151 (MQ=255)
                                                     cgcCACCTGTTCCTGGCCGGCGAGAAGCTCGCCAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCTTTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGAcc                                                                                              <  1:3150989/151‑1 (MQ=255)
                                                      gcCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCt                                                                                             >  2:10345119/1‑151 (MQ=255)
                                                       ccACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTg                                                                                            >  2:2479444/1‑151 (MQ=255)
                                                         aCCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCg                                                                                          <  1:7298332/151‑1 (MQ=255)
                                                          ccTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCgg                                                                                         <  2:1556800/151‑1 (MQ=255)
                                                           cTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCggg                                                                                        >  1:2418360/1‑151 (MQ=255)
                                                            tGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGc                                                                                       >  1:10531857/1‑151 (MQ=255)
                                                             gTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGcc                                                                                      <  1:9423596/151‑1 (MQ=255)
                                                              ttCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCg                                                                                     >  2:10544172/1‑151 (MQ=255)
                                                               tCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCgg                                                                                    <  2:7669069/151‑1 (MQ=255)
                                                                 cTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGtt                                                                                  >  2:6747444/1‑151 (MQ=255)
                                                                 cTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCGGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGtt                                                                                  <  1:8097730/151‑1 (MQ=255)
                                                                  tGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTc                                                                                 <  1:7221649/151‑1 (MQ=255)
                                                                   ggCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCg                                                                                >  2:9017063/1‑151 (MQ=255)
                                                                     ccGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGAc                                                                              <  2:520002/151‑1 (MQ=255)
                                                                      cGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGAcc                                                                             >  2:2998807/1‑151 (MQ=255)
                                                                       ggCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCa                                                                            <  1:2304059/151‑1 (MQ=255)
                                                                        gCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCaa                                                                           <  2:5275971/151‑1 (MQ=255)
                                                                          gagaAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAAcc                                                                         <  1:203324/151‑1 (MQ=255)
                                                                          gagaAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAAcc                                                                         >  2:7113804/1‑151 (MQ=255)
                                                                          gagaAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAAcc                                                                         >  2:3273835/1‑151 (MQ=255)
                                                                              aGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACcgcgc                                                                     >  1:2203042/1‑151 (MQ=255)
                                                                                 tCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGt                                                                  >  1:5937295/1‑151 (MQ=255)
                                                                                 tCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGt                                                                  <  1:7365824/151‑1 (MQ=255)
                                                                                 tCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGt                                                                  <  1:8621912/151‑1 (MQ=255)
                                                                                   gACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGc                                                                >  2:404012/1‑151 (MQ=255)
                                                                                     cagcagCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGccc                                                              >  2:5547310/1‑151 (MQ=255)
                                                                                     cagcagCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGccc                                                              >  1:10778819/1‑151 (MQ=255)
                                                                                      agcagcACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCg                                                             >  2:2283641/1‑151 (MQ=255)
                                                                                      agcagcACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCg                                                             <  1:9996245/151‑1 (MQ=255)
                                                                                       gcagcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCgg                                                            >  2:8737736/1‑151 (MQ=255)
                                                                                             cccAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCa                                                      <  1:5041549/151‑1 (MQ=255)
                                                                                                aGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACga                                                   >  1:6494267/1‑150 (MQ=255)
                                                                                                      ggctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGt                                             <  2:9089235/151‑1 (MQ=255)
                                                                                                         tggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCt                                          >  1:6184902/1‑151 (MQ=255)
                                                                                                          ggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGCCCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGCCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTg                                         <  1:3444406/151‑1 (MQ=255)
                                                                                                              gagCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgacg                                     >  1:9311104/1‑151 (MQ=255)
                                                                                                              gagCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgacg                                     <  1:7598873/151‑1 (MQ=255)
                                                                                                               agCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgacga                                    >  1:6771880/1‑151 (MQ=255)
                                                                                                                 cacaCCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGacgacg                                  >  2:9346939/1‑151 (MQ=255)
                                                                                                                  acacCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGAcgacga                                 >  2:6419276/1‑151 (MQ=255)
                                                                                                                     ccGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAgg                              <  1:11574886/151‑1 (MQ=255)
                                                                                                                       gggCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggcc                            <  1:4896338/151‑1 (MQ=255)
                                                                                                                        ggcggcCGGTCCACGACCATTGGTGGCAGACCGAGTCCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccg                           <  2:349504/151‑1 (MQ=255)
                                                                                                                           ggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccggcc                        <  2:3152056/151‑1 (MQ=255)
                                                                                                                           ggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccggcc                        <  1:10137530/151‑1 (MQ=255)
                                                                                                                            gcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGgccggccg                       <  1:2638226/151‑1 (MQ=255)
                                                                                                                              cGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCt                     >  1:392471/1‑151 (MQ=255)
                                                                                                                                    aCGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGc               <  2:9921731/151‑1 (MQ=255)
                                                                                                                                    aCGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGc               <  2:10146961/151‑1 (MQ=255)
                                                                                                                                    aCGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGc               >  1:9498898/1‑151 (MQ=255)
                                                                                                                                    aCGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGc               >  1:5173865/1‑151 (MQ=255)
                                                                                                                                     cGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCg              >  1:9120626/1‑151 (MQ=255)
                                                                                                                                        ccATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAAc           >  1:9659496/1‑151 (MQ=255)
                                                                                                                                        ccATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAAc           <  1:1273056/151‑1 (MQ=255)
                                                                                                                                         cATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAAcc          >  2:2843271/1‑151 (MQ=255)
                                                                                                                                            tggtggCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGcc       <  1:170323/151‑1 (MQ=255)
                                                                                                                                              gtggCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAg     <  1:10702352/151‑1 (MQ=255)
                                                                                                                                                ggCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAggg   <  1:8905568/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAAACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAGGGC  >  NC_008463/1558057‑1558352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑