Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,149,425 T→C Q549Q (CAA→CAG)  PA14_RS09965 ← M14 family metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,149,4250TC100.0% 1870.1 / NA 474Q549Q (CAA→CAG) PA14_RS09965M14 family metallopeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (231/243);  total (231/243)

Only 100 of 474 total aligned reads displayed.

CGCGCAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTTTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTTG  >  NC_008463/2149277‑2149572
                                                                                                                                                    |                                                                                                                                                   
cgcgCAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTg                                                                                                                                                   <  1:72441/151‑1 (MQ=255)
cgcgCAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTg                                                                                                                                                   <  2:11594307/151‑1 (MQ=255)
   gcAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCgg                                                                                                                                                <  1:4835265/151‑1 (MQ=255)
     aGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGtt                                                                                                                                              <  1:6943528/151‑1 (MQ=255)
     aGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGtt                                                                                                                                              <  1:420356/151‑1 (MQ=255)
     aGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGtt                                                                                                                                              >  2:6442519/1‑151 (MQ=255)
        cGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgca                                                                                                                                           >  1:10709591/1‑151 (MQ=255)
          tttGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcagc                                                                                                                                         <  1:10177552/151‑1 (MQ=255)
          tttGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcagc                                                                                                                                         >  2:9560308/1‑151 (MQ=255)
                gAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCg                                                                                                                                   <  1:5590334/151‑1 (MQ=255)
                gAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCg                                                                                                                                   <  2:9156609/151‑1 (MQ=255)
                     cGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGc                                                                                                                              <  2:597461/151‑1 (MQ=255)
                     cGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGc                                                                                                                              <  1:11269715/151‑1 (MQ=255)
                      ggACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGcc                                                                                                                             <  1:5383295/151‑1 (MQ=255)
                      ggACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGcc                                                                                                                             >  1:11047675/1‑151 (MQ=255)
                        aCTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAg                                                                                                                           <  2:8061015/151‑1 (MQ=255)
                         cTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAgg                                                                                                                          <  2:9473201/151‑1 (MQ=255)
                          ttCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGa                                                                                                                         <  2:3928156/151‑1 (MQ=255)
                            cAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACg                                                                                                                       <  2:1705123/151‑1 (MQ=255)
                                gcCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCa                                                                                                                   >  1:1791981/1‑151 (MQ=255)
                                gcCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCa                                                                                                                   >  1:4715071/1‑151 (MQ=255)
                                  cGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGc                                                                                                                 <  1:8113791/151‑1 (MQ=255)
                                  cGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGc                                                                                                                 >  1:3917429/1‑151 (MQ=255)
                                   gggTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCg                                                                                                                >  1:7995696/1‑151 (MQ=255)
                                       cTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTg                                                                                                            >  2:3482884/1‑151 (MQ=255)
                                          ccGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGc                                                                                                         >  2:414168/1‑151 (MQ=255)
                                            gCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCgg                                                                                                       <  1:2939996/151‑1 (MQ=255)
                                              cAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGgtg                                                                                                     >  1:9539321/1‑151 (MQ=255)
                                                     ttGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCGCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtcgatcg                                                                                              <  2:2113766/151‑1 (MQ=255)
                                                      tGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtcgatcga                                                                                             >  1:4473711/1‑151 (MQ=255)
                                                         gcgcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGACTCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGAGTCGAGCGACAg                                                                                          >  2:11232455/1‑151 (MQ=255)
                                                          cgcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGc                                                                                         >  2:5359347/1‑151 (MQ=255)
                                                             cATCGGCTCCATCAAGTAGACCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAAACCGGACTCTGCGGTTGCAGCAGGTCGAGGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAg                                                                                      >  2:6117737/1‑151 (MQ=255)
                                                              aTCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgc                                                                                     <  1:9829158/151‑1 (MQ=255)
                                                              aTCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgc                                                                                     >  1:2686074/1‑151 (MQ=255)
                                                                   cTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccggcc                                                                                <  2:691772/151‑1 (MQ=255)
                                                                   cTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccggcc                                                                                <  2:697860/151‑1 (MQ=255)
                                                                     ccATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGccggccgg                                                                              >  2:130389/1‑151 (MQ=255)
                                                                       aTCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGa                                                                            <  2:1458154/151‑1 (MQ=255)
                                                                       aTCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGa                                                                            >  2:7175437/1‑151 (MQ=255)
                                                                       aTCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGa                                                                            <  2:7582298/151‑1 (MQ=255)
                                                                       aTCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGa                                                                            <  1:10777195/151‑1 (MQ=255)
                                                                         cACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGGGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAg                                                                          >  2:3128387/1‑151 (MQ=255)
                                                                            gTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTc                                                                       >  1:10424936/1‑151 (MQ=255)
                                                                             tATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCt                                                                      <  2:10172821/151‑1 (MQ=255)
                                                                                tcctccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCa                                                                   >  2:9745566/1‑151 (MQ=255)
                                                                                   tccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAgc                                                                <  1:1262265/151‑1 (MQ=255)
                                                                                      ggCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCt                                                             >  2:10380220/1‑151 (MQ=255)
                                                                                       gCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTg                                                            >  2:8943291/1‑151 (MQ=255)
                                                                                       gCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTg                                                            >  1:830424/1‑151 (MQ=255)
                                                                                         tcctcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGa                                                          >  1:6014579/1‑151 (MQ=255)
                                                                                         tcctcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGa                                                          >  1:9144853/1‑151 (MQ=255)
                                                                                           ctcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAAc                                                        >  1:11445999/1‑151 (MQ=255)
                                                                                           ctcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAAc                                                        >  2:5763404/1‑151 (MQ=255)
                                                                                            cccgcggcCCCCAGGGTGGAATTGACGAAGCCCCAGGACCAGAACGCATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACg                                                       <  2:7770241/148‑1 (MQ=255)
                                                                                               gcggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGAGGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCt                                                    <  2:2328465/151‑1 (MQ=255)
                                                                                                 ggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTc                                                  >  2:432316/1‑151 (MQ=255)
                                                                                                 ggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTc                                                  >  2:542065/1‑151 (MQ=255)
                                                                                                  gcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCg                                                 <  2:10868337/151‑1 (MQ=255)
                                                                                                  gcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCg                                                 <  1:3781124/151‑1 (MQ=255)
                                                                                                   ccaccaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCgg                                                <  1:6456222/151‑1 (MQ=255)
                                                                                                    caccaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGc                                               <  1:6227122/151‑1 (MQ=255)
                                                                                                     accaGGGTGGAATTGACGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCg                                              <  2:7370037/151‑1 (MQ=255)
                                                                                                     accaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCg                                              >  2:3957375/1‑151 (MQ=255)
                                                                                                        aGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGc                                           <  1:2143638/151‑1 (MQ=255)
                                                                                                         gggTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGcc                                          >  1:8781628/1‑151 (MQ=255)
                                                                                                         gggTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGcc                                          >  1:7690211/1‑151 (MQ=255)
                                                                                                         gggTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGcc                                          >  1:7637070/1‑151 (MQ=255)
                                                                                                           gTGGAATTGAAGAAGCCCCAGGACCAGAACGCATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGc                                        <  2:6499706/151‑1 (MQ=255)
                                                                                                           gTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGc                                        <  2:5700063/151‑1 (MQ=255)
                                                                                                            tGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCt                                       <  1:1450710/151‑1 (MQ=255)
                                                                                                             ggAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTg                                      >  2:10657191/1‑151 (MQ=255)
                                                                                                              gAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGa                                     >  2:10157858/1‑151 (MQ=255)
                                                                                                              gAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGa                                     >  2:4662702/1‑151 (MQ=255)
                                                                                                               atttGAAGCAGCCCCAGGACCAGAACGAATCCGGTCTCTGCGGGTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGTCGGTGGCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGTACGGCTTCGGCGTGCCGCTGAc                                    <  2:3745959/149‑1 (MQ=255)
                                                                                                                aTTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGAcc                                   <  1:7422186/151‑1 (MQ=255)
                                                                                                                    aagaagCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACcagca                               >  1:1563089/1‑151 (MQ=255)
                                                                                                                     agaagCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACcagcag                              <  1:577997/151‑1 (MQ=255)
                                                                                                                       aagCCCCAGGACCAGAACGAAACCGGACTCTGCGGATGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGc                            <  1:3188124/151‑1 (MQ=255)
                                                                                                                        agCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCg                           <  2:4827306/151‑1 (MQ=255)
                                                                                                                          ccccAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGAc                         >  1:2385915/1‑151 (MQ=255)
                                                                                                                            ccAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCt                       >  2:8192697/1‑151 (MQ=255)
                                                                                                                             cAGGACCAGAACGCATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCtt                      <  2:2486485/151‑1 (MQ=255)
                                                                                                                               ggACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCg                    <  1:7330805/151‑1 (MQ=255)
                                                                                                                                  ccAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAg                 <  2:8729260/151‑1 (MQ=255)
                                                                                                                                    aGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAATGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGcc               <  2:832354/151‑1 (MQ=255)
                                                                                                                                     gAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGccc              <  2:4931579/151‑1 (MQ=255)
                                                                                                                                     gAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGccc              >  2:194242/1‑151 (MQ=255)
                                                                                                                                       ccGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCgg            <  1:3612873/150‑1 (MQ=255)
                                                                                                                                       aCGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCgg            >  1:667440/1‑151 (MQ=255)
                                                                                                                                        cGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGa           <  2:2153919/151‑1 (MQ=255)
                                                                                                                                         gAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGaa          >  1:2026408/1‑151 (MQ=255)
                                                                                                                                         gAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGaa          >  2:1619546/1‑151 (MQ=255)
                                                                                                                                          aaTCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAAt         <  2:11349322/151‑1 (MQ=255)
                                                                                                                                           aTCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATc        <  1:8661643/151‑1 (MQ=255)
                                                                                                                                            tCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATcc       <  1:4934703/151‑1 (MQ=255)
                                                                                                                                             ccGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCg      <  1:9630821/151‑1 (MQ=255)
                                                                                                                                               ggACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGt    >  2:2831910/1‑151 (MQ=255)
                                                                                                                                                gACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGtt   >  2:8476939/1‑151 (MQ=255)
                                                                                                                                                 aCTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTTg  <  2:5212173/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCGCAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTTTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTTG  >  NC_008463/2149277‑2149572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑