BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,103,224	2 bp→CA	coding (818‑819/1185 nt)	ftsZ ←	cell division protein FtsZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,103,224	0	A	C	100.0%	1909.4 / NA	477	P273M (CCT→CTG)	ftsZ	cell division protein FtsZ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (278/199); total (278/199)
*	NC_008463	5,103,225	0	G	A	99.4%	2038.1 / NA	477	P273M (CCT→CTG)	ftsZ	cell division protein FtsZ
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (275/198); minor base C (3/0); total (278/199)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 477 total aligned reads displayed.

CTCCAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCAGGGGACAGGTCCGGACCCGCGGTGATGTTCACCATGATGCCGCGCGCGCCCTGCAGGTTGACGTCTCCCAGCAGCGGGGTGCGGATTGCCGCCTCGGGGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCCATCA  >  NC_008463/5103080‑5103369

||

ctcCAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGAc                                                                                                                                             <  2:1147823/151‑1 (MQ=255)

ctcCAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGAc                                                                                                                                             <  1:4265528/151‑1 (MQ=255)

    aGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGt                                                                                                                                         >  2:4448090/1‑151 (MQ=255)

    aGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGt                                                                                                                                         >  1:816600/1‑151 (MQ=255)

     ggCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTc                                                                                                                                        <  1:8637482/151‑1 (MQ=255)

       cgcgcgcCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCg                                                                                                                                      <  2:6747170/151‑1 (MQ=255)

       cgcgcgcCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCg                                                                                                                                      >  1:10623073/1‑151 (MQ=255)

          gcgcCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGAc                                                                                                                                   >  2:4774995/1‑151 (MQ=255)

           cgcCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGAcc                                                                                                                                  <  2:3724640/151‑1 (MQ=255)

            gcCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGAccc                                                                                                                                 <  1:10129811/151‑1 (MQ=255)

            gcCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGAccc                                                                                                                                 >  1:5424016/1‑151 (MQ=255)

             cccAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCcg                                                                                                                                >  1:5119951/1‑151 (MQ=255)

               cAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCcgcg                                                                                                                              >  1:319123/1‑151 (MQ=255)

               cAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCcgcg                                                                                                                              >  2:660705/1‑151 (MQ=255)

                aGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCgg                                                                                                                             >  2:1940679/1‑151 (MQ=255)

                    cGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGAt                                                                                                                         <  1:7669079/151‑1 (MQ=255)

                           gACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCAcc                                                                                                                  >  1:2355718/1‑151 (MQ=255)

                           gACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCAcc                                                                                                                  >  1:3759969/1‑151 (MQ=255)

                            aCTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCa                                                                                                                 >  1:2171705/1‑151 (MQ=255)

                               aCGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCagga                                                                                                              >  2:10045651/1‑151 (MQ=255)

                               aCGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGACCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCagga                                                                                                              >  1:6985703/1‑151 (MQ=255)

                                cGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCaggat                                                                                                             <  2:1385554/151‑1 (MQ=255)

                                 ggTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCaggatg                                                                                                            >  2:2237415/1‑151 (MQ=255)

                                   tGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTTTTCGATGATGTTGCCGAGATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTTATGTTCTCCAGGATGcc                                                                                                          <  2:2730250/151‑1 (MQ=255)

                                   tGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCGTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGcc                                                                                                          <  1:6694383/151‑1 (MQ=255)

                                    gACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcg                                                                                                         <  2:3291766/151‑1 (MQ=255)

                                    gACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcg                                                                                                         <  2:3440932/151‑1 (MQ=255)

                                      cGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgcg                                                                                                       <  1:7557969/151‑1 (MQ=255)

                                           aGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACTGTGGCGTGCGCGGAAGCGAACTGTTCGATGATGTTGCCGTCATCGGAGTACTCGCCCCGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCgcgcgcgc                                                                                                  >  1:3820374/1‑151 (MQ=255)

                                            gCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACCGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGcc                                                                                                 >  2:1187518/1‑151 (MQ=255)

                                             cTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGccc                                                                                                >  1:9388020/1‑151 (MQ=255)

                                              tCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCt                                                                                               >  2:7027735/1‑151 (MQ=255)

                                              tCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCt                                                                                               >  2:392079/1‑151 (MQ=255)

                                               cATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTg                                                                                              >  1:4692649/1‑151 (MQ=255)

                                                  cgcgCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAg                                                                                           <  2:5258450/151‑1 (MQ=255)

                                                   gcgcATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAgg                                                                                          <  1:1213755/151‑1 (MQ=255)

                                                    cgcATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGt                                                                                         >  2:404617/1‑151 (MQ=255)

                                                       aTGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGa                                                                                      <  2:10784300/151‑1 (MQ=255)

                                                        tGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGAc                                                                                     <  1:3079779/151‑1 (MQ=255)

                                                          tCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGt                                                                                   >  2:5565347/1‑151 (MQ=255)

                                                          tCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGt                                                                                   <  1:1263478/151‑1 (MQ=255)

                                                            cgcgTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGtct                                                                                 <  1:2405931/151‑1 (MQ=255)

                                                             gcgTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGATCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGAGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGtctt                                                                                >  2:2479369/1‑150 (MQ=255)

                                                              cgTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGtcttc                                                                               >  1:7445936/1‑149 (MQ=255)

                                                              cgTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGtcttc                                                                               <  1:6562477/151‑3 (MQ=255)

                                                              cgTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGtcttc                                                                               <  2:9095846/151‑3 (MQ=255)

                                                              cgTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGtcttc                                                                               >  1:3025856/1‑149 (MQ=255)

                                                                tCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCca                                                                             <  1:1857231/151‑1 (MQ=255)

                                                                 cGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCcag                                                                            >  2:8731904/1‑151 (MQ=255)

                                                                  gATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCcagc                                                                           >  1:11338064/1‑151 (MQ=255)

                                                                  gATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCcagc                                                                           <  2:5987250/151‑1 (MQ=255)

                                                                   aTCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCcagca                                                                          >  2:3184442/1‑151 (MQ=255)

                                                                   aTCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCcagca                                                                          >  1:6093678/1‑151 (MQ=255)

                                                                    tCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCcagcag                                                                         >  2:8031804/1‑151 (MQ=255)

                                                                       cGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCgg                                                                      >  1:11680742/1‑151 (MQ=255)

                                                                         gTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCgggt                                                                    <  1:8474300/151‑2 (MQ=255)

                                                                         gTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCgggt                                                                    >  1:4218724/1‑150 (MQ=255)

                                                                          tGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCgggtt                                                                   >  2:6224488/1‑149 (MQ=255)

                                                                           gCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTg                                                                  >  1:10391176/1‑151 (MQ=255)

                                                                           gCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTg                                                                  <  1:3441715/151‑1 (MQ=255)

                                                                             ccACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCg                                                                >  1:5546044/1‑151 (MQ=255)

                                                                              cACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCgg                                                               <  1:4502125/151‑1 (MQ=255)

                                                                                ccTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGAt                                                             >  2:4210232/1‑151 (MQ=255)

                                                                                 cTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGAtt                                                            <  1:1002984/151‑1 (MQ=255)

                                                                                   tCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgc                                                          <  1:8076253/151‑1 (MQ=255)

                                                                                    cacaGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgcc                                                         <  2:15499/151‑1 (MQ=255)

                                                                                    cacaGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgcc                                                         >  1:1457238/1‑151 (MQ=255)

                                                                                      caGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgccgc                                                       >  1:2043123/1‑151 (MQ=255)

                                                                                       aGTGGCGTGCTCGGAAGCGAACAGTTCGATCATGTTGACGACATCGGAGTACTCGCCCAGGGACAGGACCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCAGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgccgac                                                      >  1:6165709/1‑149 (MQ=255)

                                                                                          ggCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCCGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCg                                                   >  1:2632346/1‑151 (MQ=255)

                                                                                            cGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCggt                                                 >  1:5846779/1‑150 (MQ=255)

                                                                                            cGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCggt                                                 <  1:3289402/151‑2 (MQ=255)

                                                                                             gTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCggtg                                                >  1:4939584/1‑149 (MQ=255)

                                                                                               gCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGc                                              >  1:4154200/1‑151 (MQ=255)

                                                                                               gCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGc                                              >  1:9316690/1‑151 (MQ=255)

                                                                                                 tCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCt                                            >  2:9613673/1‑151 (MQ=255)

                                                                                                  cGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTc                                           <  1:7140998/151‑1 (MQ=255)

                                                                                                    gAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTcgc                                         >  2:7403187/1‑151 (MQ=255)

                                                                                                        cGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGc                                     >  1:2997027/1‑151 (MQ=255)

                                                                                                        cGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGc                                     >  1:7726073/1‑151 (MQ=255)

                                                                                                            cTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACgg                                 >  2:1659640/1‑151 (MQ=255)

                                                                                                                tCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCg                             <  1:1551575/151‑1 (MQ=255)

                                                                                                                   atgatgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGAc                          >  2:11431347/1‑151 (MQ=255)

                                                                                                                     gatgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCg                        >  1:8842687/1‑151 (MQ=255)

                                                                                                                        gTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTg                     <  1:11425471/151‑1 (MQ=255)

                                                                                                                        gTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTg                     >  2:2857966/1‑151 (MQ=255)

                                                                                                                          tGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGAGTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGgc                   <  2:4890584/151‑1 (MQ=255)

                                                                                                                          tGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTGGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGgc                   <  2:3605458/151‑1 (MQ=255)

                                                                                                                           gCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGgcg                  >  2:8664432/1‑151 (MQ=255)

                                                                                                                             cGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCa                <  2:1042477/151‑1 (MQ=255)

                                                                                                                             cGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCa                <  2:7719569/151‑1 (MQ=255)

                                                                                                                               aCATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGc              >  1:9211397/1‑151 (MQ=255)

                                                                                                                                cATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGcc             <  2:8939562/151‑1 (MQ=255)

                                                                                                                                 aTCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCg            >  2:1101598/1‑151 (MQ=255)

                                                                                                                                   cGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGt          >  1:2811071/1‑151 (MQ=255)

                                                                                                                                    ggAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTa         >  1:9725541/1‑151 (MQ=255)

                                                                                                                                    ggAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTa         >  2:11641349/1‑151 (MQ=255)

                                                                                                                                      aGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTAcc       >  1:11518177/1‑151 (MQ=255)

                                                                                                                                      aGTACTCGCCCAGGGACAGCTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTAcc       <  2:6897344/151‑1 (MQ=255)

                                                                                                                                           tCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCcatca  >  2:9356609/1‑151 (MQ=255)

||

CTCCAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCAGGGGACAGGTCCGGACCCGCGGTGATGTTCACCATGATGCCGCGCGCGCCCTGCAGGTTGACGTCTCCCAGCAGCGGGGTGCGGATTGCCGCCTCGGGGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCCATCA  >  NC_008463/5103080‑5103369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`