Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,909,637 (G)5→4 intergenic (‑133/+14) PA14_RS17840 ← / ← brnQ DUF599 domain‑containing protein/branched‑chain amino acid transport system II carrier protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,909,6330G.100.0% 1916.4 / NA 386intergenic (‑129/+18)PA14_RS17840/brnQDUF599 domain‑containing protein/branched‑chain amino acid transport system II carrier protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (175/211);  total (175/211)

Only 100 of 420 total aligned reads displayed.

AAATGCCGAGTTCGGCGGTCATGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCTGGGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCGGGAGCTTGGCGAAGACGTCCGG  >  NC_008463/3909483‑3909779
                                                                                                                                                      |                                                                                                                                                  
aaaTGCCGAGTTCGGCGGTCATGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCTg                                                                                                                                                    >  1:1065531/1‑151 (MQ=255)
aaaTGCCGAGTTCGGCGGTCATGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCTg                                                                                                                                                    >  1:1307038/1‑151 (MQ=255)
    gCCGAGTTCGGCGGTCATGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCTggggt                                                                                                                                                <  1:506514/151‑2 (MQ=255)
       gAGTTCGGCGGTCATGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCTggggttcg                                                                                                                                             <  1:67972/151‑5 (MQ=255)
          ttCGGCGGTCATGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCt                                                                                                                                         >  2:6366346/1‑151 (MQ=255)
            cggcggTCATGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGa                                                                                                                                       >  1:7486728/1‑151 (MQ=255)
              gcggTCATGACAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACg                                                                                                                                     <  2:928813/151‑1 (MQ=255)
              gcggTCATGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACg                                                                                                                                     <  1:10142461/151‑1 (MQ=255)
                ggTCATGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGtc                                                                                                                                   >  1:378740/1‑151 (MQ=255)
                 gTCATGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGtct                                                                                                                                  <  1:892138/151‑1 (MQ=255)
                    aTGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAg                                                                                                                               >  1:6515217/1‑151 (MQ=255)
                    aTGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAg                                                                                                                               <  1:8706440/151‑1 (MQ=255)
                    aTGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAg                                                                                                                               <  1:9384418/151‑1 (MQ=255)
                       aaaaCTCCCTCTTGAGCCGGCCGGGATTCGCCCCGCCGTGGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAATAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCg                                                                                                                            <  2:5864590/151‑1 (MQ=255)
                        aaaCTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGa                                                                                                                           <  1:4702965/151‑1 (MQ=255)
                          aCTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGAcc                                                                                                                         <  1:7617273/151‑1 (MQ=255)
                          aCTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGAcc                                                                                                                         <  2:8820885/151‑1 (MQ=255)
                             cccTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCt                                                                                                                      >  1:1831922/1‑151 (MQ=255)
                               ctctTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTc                                                                                                                    <  1:8404476/151‑1 (MQ=255)
                               ctctTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTc                                                                                                                    >  2:10831483/1‑151 (MQ=255)
                                 ctTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTcgc                                                                                                                  <  1:4393032/151‑1 (MQ=255)
                                 ctTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTcgc                                                                                                                  >  1:1522932/1‑151 (MQ=255)
                                  ctGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTcgcg                                                                                                                 <  2:5660550/150‑1 (MQ=255)
                                    gAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTcgcgcg                                                                                                               >  2:150310/1‑151 (MQ=255)
                                    gAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTcgcgcg                                                                                                               >  2:5527155/1‑151 (MQ=255)
                                     aGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCgg                                                                                                              >  2:9567576/1‑151 (MQ=255)
                                       ccggccggGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCt                                                                                                            >  1:2786393/1‑151 (MQ=255)
                                       ccggccggGAATCGCCCCGCCGGTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCt                                                                                                            <  2:2720530/151‑1 (MQ=255)
                                        cggccggGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCtt                                                                                                           <  2:8087753/151‑1 (MQ=255)
                                         ggccggGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTg                                                                                                          >  2:3027134/1‑151 (MQ=255)
                                            cggGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGccc                                                                                                       >  2:4218437/1‑151 (MQ=255)
                                            cggGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGccc                                                                                                       >  1:2915559/1‑151 (MQ=255)
                                             gggAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCca                                                                                                      <  2:8919141/151‑1 (MQ=255)
                                                 aTCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCcagcag                                                                                                  <  1:6049404/151‑1 (MQ=255)
                                                 aTCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCcagcag                                                                                                  >  2:6638511/1‑151 (MQ=255)
                                                  tCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCagcagc                                                                                                 <  1:2040942/151‑1 (MQ=255)
                                                   cGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTGTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTGCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGcc                                                                                                >  2:5958026/1‑151 (MQ=255)
                                                      cccGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGAt                                                                                             <  1:3434929/151‑1 (MQ=255)
                                                      cccGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGAt                                                                                             >  1:9125519/1‑151 (MQ=255)
                                                       ccgccgTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATc                                                                                            <  1:5786858/151‑1 (MQ=255)
                                                          ccgTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCa                                                                                         <  1:3223581/151‑1 (MQ=255)
                                                          ccgTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCAGGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCa                                                                                         <  2:163004/151‑1 (MQ=255)
                                                           cgTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCaa                                                                                        >  1:1942120/1‑151 (MQ=255)
                                                           cgTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCaa                                                                                        >  2:8335891/1‑151 (MQ=255)
                                                            gttgtgTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCaaa                                                                                       <  2:4961474/151‑1 (MQ=255)
                                                                 ttCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAATGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACt                                                                                  >  2:1769211/1‑151 (MQ=255)
                                                                 ttCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACt                                                                                  <  2:2338401/151‑1 (MQ=255)
                                                                  tCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACCCGACTTTCGTCGGATCATGAAAATGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCTGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTg                                                                                 <  1:5526961/151‑1 (MQ=255)
                                                                  tCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTg                                                                                 <  2:7685478/151‑1 (MQ=255)
                                                                    gcgcCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGcc                                                                               >  2:9334078/1‑151 (MQ=255)
                                                                     cgcCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCa                                                                              >  1:8063908/1‑151 (MQ=255)
                                                                         gTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGAc                                                                          <  1:4800656/151‑1 (MQ=255)
                                                                             ggACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAgc                                                                      >  2:4754668/1‑151 (MQ=255)
                                                                               aCGCCGCTCGCCGCGGCCAGGACACGACTTACGACGGCTCATGAAAAAGGCGGCCCGGCGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTACGCGCGGCTTGCCCAGCCGCCGATCGCAAACGACTGCCAGGACCAgcgc                                                                    <  1:5562450/151‑1 (MQ=255)
                                                                                   cgcTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAATAGGCCGCCCGGAGGCGGCCTTCAT‑GGGGTACGCCTGACGTCTCAGGCGACCGATTCGCGCGGCTTGCCCAGCAGCCGATCGCAAGCGACTGCCAGGACCAGCGCAATg                                                                >  2:10139626/1‑151 (MQ=255)
                                                                                      tCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGAt                                                             <  2:1924715/151‑1 (MQ=255)
                                                                                       cgccgcGGCCAGGACACGACTTGCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATa                                                            <  2:3812657/151‑1 (MQ=255)
                                                                                          cgcgGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCg                                                         <  2:1266292/151‑1 (MQ=255)
                                                                                          cgcgGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCg                                                         <  2:4103102/151‑1 (MQ=255)
                                                                                           gcgGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCgg                                                        <  2:5371751/151‑1 (MQ=255)
                                                                                            cgGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGgc                                                       >  2:9676198/1‑151 (MQ=255)
                                                                                            cgGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGgc                                                       >  2:11096116/1‑151 (MQ=255)
                                                                                            cgGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGgc                                                       >  2:1830526/1‑151 (MQ=255)
                                                                                             ggCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGgca                                                      <  2:9478915/151‑1 (MQ=255)
                                                                                             ggCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGgca                                                      >  1:4512739/1‑151 (MQ=255)
                                                                                               ccAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGgcagc                                                    <  2:2473411/151‑1 (MQ=255)
                                                                                               ccAGGACACGACTTGCGTCAGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGgcagc                                                    <  2:7974532/151‑1 (MQ=255)
                                                                                                cAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGgcagca                                                   <  1:10927096/151‑1 (MQ=255)
                                                                                                cAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGgcagca                                                   <  1:2170289/151‑1 (MQ=255)
                                                                                                cAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGgcagca                                                   >  1:777969/1‑151 (MQ=255)
                                                                                                cAGGACACGACTTTCGGCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGgcagca                                                   <  1:1076595/151‑1 (MQ=255)
                                                                                                 aGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGcagcag                                                  <  2:3662395/151‑1 (MQ=255)
                                                                                                  ggACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCagcagc                                                 <  2:2440461/151‑1 (MQ=255)
                                                                                                    acacGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGcagcca                                               <  1:6365636/151‑1 (MQ=255)
                                                                                                     cacGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGcagccag                                              >  1:9616921/1‑151 (MQ=255)
                                                                                                      acGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGcagccagc                                             >  1:9937439/1‑151 (MQ=255)
                                                                                                        gACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAgccagcca                                           <  1:11124872/151‑1 (MQ=255)
                                                                                                          cTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAg                                         <  2:339532/151‑1 (MQ=255)
                                                                                                              cgtcgGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGctc                                     <  2:7211591/151‑1 (MQ=255)
                                                                                                               gtcgGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGctct                                    >  2:7024331/1‑151 (MQ=255)
                                                                                                                  ggCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGAt                                 >  1:5169016/1‑151 (MQ=255)
                                                                                                                   gCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATc                                >  1:8264666/1‑151 (MQ=255)
                                                                                                                    cTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCg                               >  1:1005245/1‑151 (MQ=255)
                                                                                                                      cATGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGc                             <  2:10385131/151‑1 (MQ=255)
                                                                                                                       aTGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCg                            <  1:7278153/151‑1 (MQ=255)
                                                                                                                        tGAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGa                           >  2:7788533/1‑151 (MQ=255)
                                                                                                                         gAAAAAGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAg                          >  1:5317495/1‑151 (MQ=255)
                                                                                                                          aaaaaGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGc                         >  2:681946/1‑151 (MQ=255)
                                                                                                                             aaGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCggg                      <  2:8470980/151‑1 (MQ=255)
                                                                                                                              aGGCCGCCCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGACTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCGGGa                     <  1:9780658/151‑1 (MQ=255)
                                                                                                                                 ccgccCGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCGGGAGCt                  <  2:7389927/151‑1 (MQ=255)
                                                                                                                                     ccGGAGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCGGGAGCTTGGc              <  2:4380873/151‑1 (MQ=255)
                                                                                                                                     ccGGAGGCGGCCATCCT‑GGGGTACGCCTGACTACTCAGGCGACCGCTTCGCGCGGCTTGCCCCGCAGCCGATCGCACACGACTGCCAGGACCAGCGCAATTGATACCGGCAGCAGCCAGCCCAGGCTCTGATCGGCGAGCGGGAGCTTGGc              >  2:10581050/1‑151 (MQ=255)
                                                                                                                                      cGGAGGCGGCCGTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCGGGAGCTTGGCg             >  1:2466466/1‑151 (MQ=255)
                                                                                                                                         aGGCGGCCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCGGGAGCTTGGCGAAg          <  1:4191856/151‑1 (MQ=255)
                                                                                                                                           gcggcCTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCGGGAGCTTGGCGAAGAc        >  2:1070405/1‑151 (MQ=255)
                                                                                                                                               ccTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCGGGAGCTTGGCGAAGACGTcc    >  1:6979297/1‑151 (MQ=255)
                                                                                                                                               ccTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCGGGAGCTTGGCGAAGACGTcc    <  2:905143/151‑1 (MQ=255)
                                                                                                                                                cTTCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCGGGAGCTTGGCGAAGACGTCCg   >  2:4512551/1‑151 (MQ=255)
                                                                                                                                                 ttCCT‑GGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCGGGAGCTTGGCGAAGACGTCCgg  >  1:10490613/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
AAATGCCGAGTTCGGCGGTCATGAAAACTCCCTCTTGAGCCGGCCGGGAATCGCCCCGCCGTTGTTTCGCGCCGTTGGGACGCCGCTCGCCGCGGCCAGGACACGACTTTCGTCGGCTCATGAAAAAGGCCGCCCGGAGGCGGCCTTCCTGGGGGTTCGCCTGACGTCTCAGGCGACCGCTTCGCGCGGCTTGCCCAGCAGCCGATCGCAAACGACTGCCAGGACCAGCGCAATGGATACCGGCAGCAGCCAGCCAAGGCTCTGATCGGCGAGCGGGAGCTTGGCGAAGACGTCCGG  >  NC_008463/3909483‑3909779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑