Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,700,807 Δ1 bp intergenic (+165/‑150) PA14_RS21585 → / → pbpG aspartate/tyrosine/aromatic aminotransferase/D‑alanyl‑D‑alanine endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,700,8070G.100.0% 2411.9 / NA 486intergenic (+165/‑150)PA14_RS21585/pbpGaspartate/tyrosine/aromatic aminotransferase/D‑alanyl‑D‑alanine endopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (222/264);  total (222/264)

Only 100 of 486 total aligned reads displayed.

AGCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGCGCGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCAAC  >  NC_008463/4700661‑4700952
                                                                                                                                                  |                                                                                                                                                 
aGCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAAc                                                                                                                                              >  1:1614570/1‑151 (MQ=255)
   cGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACctc                                                                                                                                           >  1:11114001/1‑151 (MQ=255)
     gCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGTGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCtctc                                                                                                                                         >  2:4576379/1‑151 (MQ=255)
      cTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTcc                                                                                                                                        >  2:7784442/1‑151 (MQ=255)
      cTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTcc                                                                                                                                        >  2:2748015/1‑151 (MQ=255)
        gtgATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGa                                                                                                                                      >  1:11047119/1‑151 (MQ=255)
        gtgATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGa                                                                                                                                      >  1:8832852/1‑151 (MQ=255)
         tgATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGaa                                                                                                                                     >  1:10714163/1‑151 (MQ=255)
          gATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAAc                                                                                                                                    <  1:3073002/151‑1 (MQ=255)
           aTGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAAcc                                                                                                                                   <  2:6662789/151‑1 (MQ=255)
                cGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCGTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGcc                                                                                                                              >  2:8687728/1‑151 (MQ=255)
                 ggCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCa                                                                                                                             >  1:373182/1‑151 (MQ=255)
                 ggCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCa                                                                                                                             >  1:6400127/1‑151 (MQ=255)
                 ggCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCa                                                                                                                             <  2:10262423/151‑1 (MQ=255)
                      tttttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCAt                                                                                                                        <  2:9346685/151‑1 (MQ=255)
                       ttttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCAtc                                                                                                                       >  1:6015907/1‑150 (MQ=255)
                          tCGCCCCCGCGAATGCCGCGCACGCCCGCCGAGGCGGCCGATAACCGCGTTGCGGTTATTCGCCCAACCGCACCACGACCGTGAGGGCTGATGGCGAATACCGCCACAGGCGATATCCGC‑CGAACCTCTCCGAACCGTGCCAACCAGAAGt                                                                                                                    >  2:10750868/1‑151 (MQ=255)
                            gCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCa                                                                                                                  >  1:4908597/1‑151 (MQ=255)
                             ccccAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAg                                                                                                                 <  2:3870080/151‑1 (MQ=255)
                               ccAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCa                                                                                                               <  1:7896900/151‑1 (MQ=255)
                                    gAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTc                                                                                                          <  2:9880660/151‑1 (MQ=255)
                                     aaTGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCa                                                                                                         >  1:4989133/1‑151 (MQ=255)
                                         ccGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGc                                                                                                     <  1:8158236/151‑1 (MQ=255)
                                           gcgcACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGccc                                                                                                   >  2:5599865/1‑151 (MQ=255)
                                             gcACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGGGCCAACCATAAGTCAGCACTTTCAAAGCCCTa                                                                                                 >  2:9119507/1‑151 (MQ=255)
                                                cTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGt                                                                                              <  1:2039426/151‑1 (MQ=255)
                                                cTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGt                                                                                              <  1:2703133/151‑1 (MQ=255)
                                                  cccGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGc                                                                                            >  1:5264548/1‑151 (MQ=255)
                                                  cccGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGc                                                                                            >  2:186131/1‑151 (MQ=255)
                                                   ccgccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCt                                                                                           <  2:3746261/151‑1 (MQ=255)
                                                   ccgccgAGGCGGCGGATAACCGCGGTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCt                                                                                           >  1:8985598/1‑151 (MQ=255)
                                                      ccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCtttt                                                                                        <  1:189721/151‑1 (MQ=255)
                                                         aGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCt                                                                                     <  2:5180688/151‑1 (MQ=255)
                                                             ggcggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTGGTGGGCGAAGGGCCAATACCGCCACAGGCGTGAGCCGC‑CGAACCTCTCCGAACCGGGCCAACCATAAGTCAGCCCTTTCAAAGCCCTTCGTGCGTATCCTACaa                                                                                 >  2:8953349/1‑151 (MQ=255)
                                                               cggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACaaaa                                                                               >  2:4266036/1‑151 (MQ=255)
                                                               cggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACaaaa                                                                               <  1:7384221/151‑1 (MQ=255)
                                                               cggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACaaaa                                                                               <  2:7617070/151‑1 (MQ=255)
                                                                ggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTCTCCGC‑CGACCCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTCCAAAag                                                                              <  2:6418636/151‑1 (MQ=255)
                                                                 gATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATACGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAaga                                                                             <  2:9518881/151‑1 (MQ=255)
                                                                 gATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAaga                                                                             >  2:7996324/1‑151 (MQ=255)
                                                                 gATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTGTTCCTACAAAaga                                                                             >  2:6607574/1‑151 (MQ=255)
                                                                  aTAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAagag                                                                            >  1:9681005/1‑151 (MQ=255)
                                                                  aTAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAagag                                                                            >  2:5099193/1‑151 (MQ=255)
                                                                   tAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCAGACAAAAGAGc                                                                           >  2:5646892/1‑151 (MQ=255)
                                                                     aCCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCAt                                                                         <  1:1768378/151‑1 (MQ=255)
                                                                     aCCGCGTTGCGGATATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGCACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCAt                                                                         <  1:3400182/151‑1 (MQ=255)
                                                                      ccGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATa                                                                        >  1:10301718/1‑151 (MQ=255)
                                                                           ttGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATg                                                                   <  2:7053134/151‑1 (MQ=255)
                                                                            tGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGc                                                                  <  1:445491/151‑1 (MQ=255)
                                                                             gCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAACTGCt                                                                 <  1:3513161/151‑1 (MQ=255)
                                                                             gCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCt                                                                 <  2:6907261/151‑1 (MQ=255)
                                                                              cGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAACAGAGCATAAAATGCTc                                                                <  2:8108273/151‑1 (MQ=255)
                                                                              cGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTc                                                                >  1:8453802/1‑151 (MQ=255)
                                                                               ggTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACACTAAGTCAGCTCTTTCAAAGCCCTACTTGCTTTTCATACAAAAGAGCATAAAATGCTCa                                                               <  2:10635038/151‑1 (MQ=255)
                                                                                 ttATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAAt                                                             <  2:5864131/151‑1 (MQ=255)
                                                                                  tATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAAtt                                                            >  1:1263312/1‑151 (MQ=255)
                                                                                  tATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAAtt                                                            >  2:2364521/1‑151 (MQ=255)
                                                                                   aTTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCACCCATAAGTCAGCACTTTCAAAGCCCTCCGTGCTTTTCCTACAAAAGAGCATAAACTGCTCAATTa                                                           <  2:821633/151‑1 (MQ=255)
                                                                                    ttCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTAt                                                          >  1:5735250/1‑151 (MQ=255)
                                                                                    ttCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTAt                                                          >  1:7131029/1‑151 (MQ=255)
                                                                                     tCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATg                                                         <  1:7193799/151‑1 (MQ=255)
                                                                                       gCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATggg                                                       <  1:4048096/151‑1 (MQ=255)
                                                                                        cccTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGc                                                      <  1:2308854/151‑1 (MQ=255)
                                                                                          cTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCAc                                                    >  2:2079980/1‑151 (MQ=255)
                                                                                           tACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCAcc                                                   <  1:461280/151‑1 (MQ=255)
                                                                                            aCCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCa                                                  <  1:7572143/151‑1 (MQ=255)
                                                                                            aCCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCa                                                  >  2:600415/1‑151 (MQ=255)
                                                                                             ccGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGCACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAg                                                 <  1:8281866/151‑1 (MQ=255)
                                                                                                caccacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTc                                              <  2:1576940/151‑1 (MQ=255)
                                                                                                caccacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGAGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTc                                              >  2:1356821/1‑151 (MQ=255)
                                                                                                 accacGACCTTGTGGGCGTAGGGCGAACACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAACATGCTCAATTATGGGCACCAGTTca                                             <  1:59884/151‑1 (MQ=255)
                                                                                                   cacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTcaca                                           <  2:4434578/151‑1 (MQ=255)
                                                                                                    acGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCacac                                          <  1:1457108/151‑1 (MQ=255)
                                                                                                      gACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTg                                        <  2:10053285/151‑1 (MQ=255)
                                                                                                         cTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCTCACTgcgc                                     >  2:6749960/1‑151 (MQ=255)
                                                                                                           tgtgGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGccc                                   <  2:10200846/151‑1 (MQ=255)
                                                                                                            gtgGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGcccc                                  >  1:10139329/1‑151 (MQ=255)
                                                                                                            gtgGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGcccc                                  >  2:10944866/1‑151 (MQ=255)
                                                                                                              gggCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCAc                                <  1:481316/151‑1 (MQ=255)
                                                                                                               ggCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACg                               <  2:2566759/151‑1 (MQ=255)
                                                                                                               ggCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCAACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACg                               >  1:6821109/1‑151 (MQ=255)
                                                                                                                gCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACgg                              >  2:4959265/1‑151 (MQ=255)
                                                                                                                gCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACgg                              <  1:8649948/151‑1 (MQ=255)
                                                                                                                        cGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACt                      >  1:6077705/1‑151 (MQ=255)
                                                                                                                        cGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACt                      >  2:11035131/1‑151 (MQ=255)
                                                                                                                         gAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACtt                     >  2:4477417/1‑151 (MQ=255)
                                                                                                                         gAATACCGACACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACtt                     <  2:7250282/151‑1 (MQ=255)
                                                                                                                           aTACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTtc                   <  2:7453339/151‑1 (MQ=255)
                                                                                                                             aCCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTtctc                 >  1:3240860/1‑151 (MQ=255)
                                                                                                                                 ccACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCt             <  1:9799285/151‑1 (MQ=255)
                                                                                                                                  cacaGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCtt            <  1:9446781/151‑1 (MQ=255)
                                                                                                                                  cacaGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCtt            <  1:10526478/151‑1 (MQ=255)
                                                                                                                                   acaGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTa           <  2:8412163/151‑1 (MQ=255)
                                                                                                                                   acaGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTa           <  1:5528078/151‑1 (MQ=255)
                                                                                                                                    caGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAg          <  2:3101730/151‑1 (MQ=255)
                                                                                                                                     aGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATACGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAACATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGt         <  2:6218133/151‑1 (MQ=255)
                                                                                                                                     aGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGt         >  2:4118501/1‑151 (MQ=255)
                                                                                                                                      ggCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTg        <  2:5328309/151‑1 (MQ=255)
                                                                                                                                         gTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGc     <  2:1152646/151‑1 (MQ=255)
                                                                                                                                           tATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCaa   <  2:3974904/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGCGCGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCAAC  >  NC_008463/4700661‑4700952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑