Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,441,861 (C)6→4 intergenic (+31/+11) mexK → / ← PA14_RS06750 multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,441,8570C.99.7% 1924.0 / ‑2.0 390intergenic (+27/+16)mexK/PA14_RS06750multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (173/216);  total (173/217)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
*NC_0084631,441,8580C.99.7% 1923.2 / ‑2.6 390intergenic (+28/+15)mexK/PA14_RS06750multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (173/216);  minor base G (0/1);  total (173/217)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 448 total aligned reads displayed.

TTCGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGCCCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCTCG  >  NC_008463/1441708‑1442005
                                                                                                                                                     ||                                                                                                                                                   
ttcGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGcc                                                                                                                                                     >  2:3382252/1‑151 (MQ=255)
  cGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCCCCCTGCTGGTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGACGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGcccc                                                                                                                                                   <  2:10336322/151‑1 (MQ=255)
  cGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGcccc                                                                                                                                                   <  2:10526062/151‑1 (MQ=255)
    ggCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgt                                                                                                                                                 >  2:7354364/1‑149 (MQ=255)
     gCCGCTGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGCGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtc                                                                                                                                                <  2:8002982/151‑4 (MQ=255)
      ccGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtcc                                                                                                                                               >  1:686029/1‑147 (MQ=255)
       cGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCGGCCGGCGCTCGATGCCGCCTGGTTCCGTGTGAAGACGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccg                                                                                                                                              >  2:7320074/1‑146 (MQ=255)
        gATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccgt                                                                                                                                             >  1:2503342/1‑145 (MQ=255)
          tGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCg                                                                                                                                         <  1:6502870/151‑1 (MQ=255)
              ggTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTc                                                                                                                                     >  1:1565467/1‑151 (MQ=255)
              ggTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTc                                                                                                                                     >  1:6111582/1‑151 (MQ=255)
                  gCGATCATGGGCGGGCTGATCGTCTCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCt                                                                                                                                 >  1:5147159/1‑151 (MQ=255)
                   cGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTg                                                                                                                                <  1:8668942/151‑1 (MQ=255)
                   cGATCATGGGCGGGCTGATCGTCGCCACCGAGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTg                                                                                                                                >  1:4201801/1‑151 (MQ=255)
                      tCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGcc                                                                                                                             >  1:9087965/1‑151 (MQ=255)
                        aTGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCcgc                                                                                                                           >  1:35103/1‑151 (MQ=255)
                         tGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCGGTGTGAAGAAGGACGAGGCGCGGGCCTGTCGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCcgcg                                                                                                                          >  2:8986759/1‑151 (MQ=255)
                         tGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCCCCATCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCcgcg                                                                                                                          >  2:11132930/1‑151 (MQ=255)
                                gcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGa                                                                                                                   >  2:9696983/1‑151 (MQ=255)
                                 cTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGAc                                                                                                                  >  1:1334692/1‑151 (MQ=255)
                                 cTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGAc                                                                                                                  <  2:8176702/151‑1 (MQ=255)
                                   gATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACct                                                                                                                >  1:1000591/1‑151 (MQ=255)
                                      cgtcgCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACcttct                                                                                                             <  1:1493397/151‑1 (MQ=255)
                                       gtcgCCACCGTGCTCACCCTGCGGGTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACcttctt                                                                                                            <  1:10694938/151‑1 (MQ=255)
                                         cgCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTgg                                                                                                          <  2:1543073/151‑1 (MQ=255)
                                          gCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGc                                                                                                         >  2:9567729/1‑151 (MQ=255)
                                           ccACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGcc                                                                                                        <  1:5544518/151‑1 (MQ=255)
                                            cACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCa                                                                                                       <  2:5834468/151‑1 (MQ=255)
                                            cACCGTGCTCACCCTGCTGTTCCTGCCGGCGCACTATGCCGCCTGGGTCCGTGTGAAGACGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCa                                                                                                       <  1:91769/151‑1 (MQ=255)
                                             aCCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGACGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAg                                                                                                      <  1:99996/151‑1 (MQ=255)
                                             aCCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAg                                                                                                      >  1:258678/1‑151 (MQ=255)
                                               cGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGc                                                                                                    <  2:7586473/151‑1 (MQ=255)
                                                gTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGcc                                                                                                   <  2:9875674/151‑1 (MQ=255)
                                                  gCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCgg                                                                                                 <  1:7771249/151‑1 (MQ=255)
                                                    tCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTg                                                                                               >  1:9722394/1‑151 (MQ=255)
                                                     cACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTgg                                                                                              >  1:7613151/1‑151 (MQ=255)
                                                      aCCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTggc                                                                                             <  1:7063295/151‑1 (MQ=255)
                                                          tgctgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgcc                                                                                         >  2:5564093/1‑151 (MQ=255)
                                                            ctgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgccgc                                                                                       <  1:3636610/151‑1 (MQ=255)
                                                            ctgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgccgc                                                                                       <  2:851118/151‑1 (MQ=255)
                                                             tgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgccgcc                                                                                      <  1:4125159/151‑1 (MQ=255)
                                                                   tGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCggg                                                                                >  1:4674342/1‑151 (MQ=255)
                                                                   tGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTAGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGAGGCGGCCGCCGCCggg                                                                                >  2:5445802/1‑151 (MQ=255)
                                                                   tGCCGGCGCTCTAGGCCGCCTGGTTCCGGGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCggg                                                                                <  2:3977843/151‑1 (MQ=255)
                                                                      cGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTc                                                                             >  2:3618988/1‑151 (MQ=255)
                                                                       ggCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCt                                                                            >  1:231520/1‑151 (MQ=255)
                                                                          gcTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGc                                                                         <  1:10357889/151‑1 (MQ=255)
                                                                           ctctATGCCGCCTGGTTCCGTGTGAAGAAGGCCGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCg                                                                        <  2:9434729/151‑1 (MQ=255)
                                                                            tctATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCgg                                                                       <  1:3962190/151‑1 (MQ=255)
                                                                            tctATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCgg                                                                       >  2:665435/1‑151 (MQ=255)
                                                                               aTGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATg                                                                    >  1:3001371/1‑151 (MQ=255)
                                                                                    gccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGCCGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtctt                                                               <  1:3179451/151‑1 (MQ=255)
                                                                                    gccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtctt                                                               >  2:7537/1‑151 (MQ=255)
                                                                                     ccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtcttc                                                              <  1:4925669/151‑1 (MQ=255)
                                                                                      cTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtcttct                                                             >  2:2862711/1‑151 (MQ=255)
                                                                                         gTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCt                                                          >  2:4667734/1‑151 (MQ=255)
                                                                                         gTTCCGTGTGAAGAAGGACGAGGCGCGGGCCGGACGGGAGATGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCt                                                          <  1:67458/151‑1 (MQ=255)
                                                                                            ccGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGA‑‑GCGCGCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCt                                                       <  2:8420614/151‑1 (MQ=255)
                                                                                             cGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCtt                                                      <  1:11120351/151‑1 (MQ=255)
                                                                                             cGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCtt                                                      <  2:4106588/151‑1 (MQ=255)
                                                                                              gtgtGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCACTTCTCCTGCTTg                                                     <  1:10160001/151‑1 (MQ=255)
                                                                                                 tgAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCg                                                  >  1:7552366/1‑151 (MQ=255)
                                                                                                  gaagaaGGACGAGGCGCGGGCCTGCCGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCgc                                                 <  1:1670812/151‑2 (MQ=255)
                                                                                                  gaagaaGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGa                                                 >  2:51245/1‑151 (MQ=255)
                                                                                                   aagaagGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGat                                                <  2:7124888/151‑1 (MQ=255)
                                                                                                   aagaagGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTACTGCTTGGCGat                                                >  2:10777168/1‑151 (MQ=255)
                                                                                                     gaagGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGatca                                              <  2:10431309/151‑1 (MQ=255)
                                                                                                     gaagGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGatca                                              >  2:175084/1‑151 (MQ=255)
                                                                                                     gaagGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGACGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGatca                                              <  1:10791853/151‑1 (MQ=255)
                                                                                                      aagGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGatcat                                             <  2:3756490/151‑1 (MQ=255)
                                                                                                          aCGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCg                                         <  1:2130935/151‑1 (MQ=255)
                                                                                                          aCGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCg                                         <  2:4894106/151‑1 (MQ=255)
                                                                                                          aCGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCGTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCg                                         >  1:579696/1‑151 (MQ=255)
                                                                                                            gAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGaa                                       <  1:4388977/151‑1 (MQ=255)
                                                                                                              ggCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAaga                                     <  1:10959874/151‑1 (MQ=255)
                                                                                                              ggCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAaga                                     <  1:3286685/151‑1 (MQ=255)
                                                                                                                cgcgGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACGCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCGCTTCTACTGCTTTGCGATCATCTCGAAGAGc                                   >  1:340658/1‑151 (MQ=255)
                                                                                                                 gcgGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGATCTTCTCCTGCTTGGCGATCATCTCGAAGAGcc                                  >  1:3779354/1‑151 (MQ=255)
                                                                                                                   gggCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCg                                >  1:6267482/1‑151 (MQ=255)
                                                                                                                    ggCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGt                               >  1:8816439/1‑151 (MQ=255)
                                                                                                                      ccTGACGGGAGACGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCg                             <  1:2399644/151‑1 (MQ=255)
                                                                                                                         gACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAgg                          <  2:1316073/151‑1 (MQ=255)
                                                                                                                         gACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAgg                          <  1:6995671/151‑1 (MQ=255)
                                                                                                                          aCGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAggg                         >  1:9694094/1‑151 (MQ=255)
                                                                                                                            gggAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGcc                       <  2:2707368/151‑1 (MQ=255)
                                                                                                                            gggAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCAGGCGGATGCTCTACTCCTGCTTGGCGCTCATCTCGAAGAGCCCGTCGAGGGcc                       >  2:6242966/1‑151 (MQ=255)
                                                                                                                             ggAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCt                      <  1:8513571/151‑1 (MQ=255)
                                                                                                                                gaAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGtg                   >  1:833927/1‑150 (MQ=255)
                                                                                                                                 aaGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTc                  <  1:2680377/151‑1 (MQ=255)
                                                                                                                                    gggACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGt               <  2:7732592/151‑1 (MQ=255)
                                                                                                                                    gggACGGCGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGt               <  1:600065/151‑1 (MQ=255)
                                                                                                                                       aCGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGACGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGAc            <  2:1434174/151‑1 (MQ=255)
                                                                                                                                         ggggCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGACGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACAt          <  1:6069488/151‑1 (MQ=255)
                                                                                                                                         ggggCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACAt          <  2:2219223/151‑1 (MQ=255)
                                                                                                                                              gcgatcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGc       >  1:7239169/6‑151 (MQ=255)
                                                                                                                                              gcgatcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGc       <  1:5430737/146‑1 (MQ=255)
                                                                                                                                                gatcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGccc     >  2:6477861/4‑151 (MQ=255)
                                                                                                                                                 atcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCt    <  1:7322363/149‑1 (MQ=255)
                                                                                                                                                  tcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCTc   <  2:2475678/150‑1 (MQ=255)
                                                                                                                                                  tcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCTc   <  1:2921620/150‑1 (MQ=255)
                                                                                                                                                     ||                                                                                                                                                   
TTCGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGCCCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCTCG  >  NC_008463/1441708‑1442005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑