Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,341,704 Δ1 bp pseudogene (664/957 nt) PA14_RS10890 → DUF58 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,341,7020C.98.5% 2306.4 / 17.9 480pseudogene (662/957 nt)PA14_RS10890DUF58 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (6/1);  new base . (239/233);  total (246/234)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 503 total aligned reads displayed.

GAGCTGGGTCGACCTGCGCCCAGCAGGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAGCCCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTACCCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGAAAGTCGCCTGGGC  >  NC_008463/2341550‑2341850
                                                                                                                                                        |                                                                                                                                                    
gAGCTGGGTCGACCTGCG‑CCAGCAGGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCGGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTAc                                                                                                                                                      >  1:8567148/1‑151 (MQ=255)
  gCTGGGTCGACCTGCG‑CCAGCAGGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTAcca                                                                                                                                                    <  2:7360142/151‑2 (MQ=255)
  gCTGGGTCGACCTGCG‑CCAGCAGGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGGTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTAcca                                                                                                                                                    <  1:4623591/151‑2 (MQ=255)
   cTGGGTCGACCTGCG‑CCAGCAGGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTAccag                                                                                                                                                   <  1:4466323/151‑3 (MQ=255)
   cTGGGTCGACCTGCG‑CCAGCAGGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGAGGCCGAGGACCAGGGGCGCGCGCTGGCGGGCACGGGCGTCGACGACTACCAGGGCCTGCGCGCCTAccgg                                                                                                                                                   >  1:8015416/1‑149 (MQ=255)
    tGGGTCGACCTGCG‑CCAGCAGGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTAccagc                                                                                                                                                  <  2:7780208/151‑4 (MQ=255)
     gggTCGACCTGCG‑CCAGCAGGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTAccagcc                                                                                                                                                 <  1:7130461/151‑5 (MQ=255)
      ggTCGACCTGCG‑CCAGCAGGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCg                                                                                                                                               <  1:7373608/151‑1 (MQ=255)
          gACCTGCG‑CCAGCAGGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCg                                                                                                                                           <  1:5938792/151‑1 (MQ=255)
                    cagcagGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAGCCC‑TTCCTCGCGCCGGGCGCGCAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACGACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCgcggc                                                                                                                                  <  1:1148062/151‑1 (MQ=255)
                    cagcagGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCgcggc                                                                                                                                  <  1:6452034/151‑1 (MQ=255)
                      gcagGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCgcggcgg                                                                                                                                >  1:2230467/1‑151 (MQ=255)
                       cagGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCgcggcggc                                                                                                                               >  2:5132194/1‑151 (MQ=255)
                        agGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCACGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACGACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCgcggcggcg                                                                                                                              >  2:828770/1‑151 (MQ=255)
                          gcgcTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGcc                                                                                                                            <  1:2880422/151‑1 (MQ=255)
                            gcTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTg                                                                                                                          <  2:6955272/151‑1 (MQ=255)
                             cTGGTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGc                                                                                                                         >  1:7498104/1‑151 (MQ=255)
                               ggTCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCAc                                                                                                                       <  1:8938248/151‑1 (MQ=255)
                                 tCTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGTGGACCCGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCCGGGACTGCGCGCCT‑CCCAGCCGGGCGACTCGCGGCGGCGCCTGCACTg                                                                                                                     >  2:1127354/1‑151 (MQ=255)
                                  cTACCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTgg                                                                                                                    <  1:1990137/151‑1 (MQ=255)
                                    aCCCTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGaa                                                                                                                  >  2:181177/1‑151 (MQ=255)
                                       cTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGc                                                                                                               >  2:2844970/1‑151 (MQ=255)
                                       cTCGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGc                                                                                                               >  1:9089542/1‑151 (MQ=255)
                                         cGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGt                                                                                                             <  1:7004523/151‑1 (MQ=255)
                                         cGGCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGt                                                                                                             <  2:3758141/151‑1 (MQ=255)
                                           gCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGttt                                                                                                           >  1:5491796/1‑151 (MQ=255)
                                           gCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGttt                                                                                                           >  2:5409874/1‑151 (MQ=255)
                                           gCCGCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGttt                                                                                                           <  2:5711090/151‑1 (MQ=255)
                                              gCTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTcc                                                                                                        >  2:3391003/1‑151 (MQ=255)
                                               cTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTccc                                                                                                       <  1:1000543/151‑1 (MQ=255)
                                               cTGGCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACGGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTccc                                                                                                       >  1:3823907/1‑151 (MQ=255)
                                                 ggCCGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGt                                                                                                     <  2:2424752/151‑1 (MQ=255)
                                                   ccGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTgg                                                                                                   >  2:4636406/1‑151 (MQ=255)
                                                   ccGGCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTgg                                                                                                   <  2:750047/151‑1 (MQ=255)
                                                      gCGAG‑CCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCa                                                                                                <  2:885534/151‑1 (MQ=255)
                                                        cgagccGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAg                                                                                               >  1:10816163/5‑151 (MQ=255)
                                                         gagccGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAgg                                                                                              >  1:5829792/4‑151 (MQ=255)
                                                            ccGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGgc                                                                                           >  2:3637427/1‑151 (MQ=255)
                                                            ccGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGgc                                                                                           >  2:7017580/1‑151 (MQ=255)
                                                            ccGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGgc                                                                                           >  2:2016987/1‑151 (MQ=255)
                                                             cGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGgct                                                                                          <  2:8920416/151‑1 (MQ=255)
                                                              gTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGgctg                                                                                         >  1:6534422/1‑151 (MQ=255)
                                                               gtCCTCGCGCCGGGCGCGGAGGATGCCGCGGACCCGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACGACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTGGTCCCGTGGCCAGGGgctgc                                                                                        <  2:10010293/150‑1 (MQ=255)
                                                                tCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGgctgct                                                                                       <  2:9532198/151‑1 (MQ=255)
                                                                 ccTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGctgctg                                                                                      <  2:8531869/151‑1 (MQ=255)
                                                                     gcgcCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGa                                                                                  <  2:9311233/151‑1 (MQ=255)
                                                                      cgcCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGaa                                                                                 >  1:6227550/1‑151 (MQ=255)
                                                                      cgcCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGaa                                                                                 <  1:1434790/151‑1 (MQ=255)
                                                                           ggCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACt                                                                            >  1:4753677/1‑151 (MQ=255)
                                                                              gcgGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTcg                                                                         <  2:3963800/151‑1 (MQ=255)
                                                                              gcgGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTcg                                                                         >  2:10437330/1‑151 (MQ=255)
                                                                               cgGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTcgc                                                                        <  1:4240195/151‑1 (MQ=255)
                                                                                ggaggaTGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGACGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTcgcc                                                                       >  1:8167862/1‑151 (MQ=255)
                                                                                     aTGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCt                                                                  >  1:2540840/1‑151 (MQ=255)
                                                                                      tGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTc                                                                 >  1:8404446/1‑151 (MQ=255)
                                                                                       gCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACGACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTcg                                                                <  2:4201098/151‑1 (MQ=255)
                                                                                        ccGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTcgg                                                               >  1:10342280/1‑151 (MQ=255)
                                                                                            ggACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCggg                                                           >  1:5304549/1‑151 (MQ=255)
                                                                                              aCCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGa                                                         >  2:10103166/1‑151 (MQ=255)
                                                                                               ccAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGaa                                                        <  1:321096/151‑1 (MQ=255)
                                                                                                cAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAg                                                       >  1:10562766/1‑151 (MQ=255)
                                                                                                   gggCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGAcc                                                    >  1:776530/1‑151 (MQ=255)
                                                                                                    ggCGCGCGCGGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCt                                                   <  1:4658948/151‑1 (MQ=255)
                                                                                                       gcgcgcTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCtgtg                                                >  1:1022217/1‑151 (MQ=255)
                                                                                                           gcTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTg                                            >  2:4271395/1‑151 (MQ=255)
                                                                                                             tGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGa                                          >  1:2808582/1‑151 (MQ=255)
                                                                                                             tGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGa                                          >  2:10181309/1‑151 (MQ=255)
                                                                                                             tGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGa                                          >  1:3472906/1‑151 (MQ=255)
                                                                                                                 gAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCGGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTc                                      >  1:2645414/1‑151 (MQ=255)
                                                                                                                   gCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGa                                    <  1:2368172/151‑1 (MQ=255)
                                                                                                                   gCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGa                                    <  2:9399647/151‑1 (MQ=255)
                                                                                                                    cACGGGCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAg                                   >  1:6669842/1‑151 (MQ=255)
                                                                                                                        ggCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGcc                               <  2:6145303/151‑1 (MQ=255)
                                                                                                                         gCGTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCt                              >  2:11088674/1‑151 (MQ=255)
                                                                                                                           gTCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTcg                            <  1:9290859/151‑1 (MQ=255)
                                                                                                                            tCGACGACTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTcgg                           <  1:9613738/151‑1 (MQ=255)
                                                                                                                             cgacgaCTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGTAGGCGTGTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTcggc                          <  1:4868678/151‑1 (MQ=255)
                                                                                                                              gacgacTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTcggcg                         <  2:3030010/151‑1 (MQ=255)
                                                                                                                              gacgacTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTcggcg                         >  2:6714247/1‑151 (MQ=255)
                                                                                                                              gacgacTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTcggcg                         <  2:2163401/151‑1 (MQ=255)
                                                                                                                               acgacTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTcggcgg                        <  2:1596274/151‑1 (MQ=255)
                                                                                                                                   cgaCCAGGGACCGCGCGCC‑CCCCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGAt                    <  2:7095118/149‑1 (MQ=255)
                                                                                                                                   cTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGAt                    >  1:3539275/1‑151 (MQ=255)
                                                                                                                                   cTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGAt                    >  1:989746/1‑151 (MQ=255)
                                                                                                                                   cTACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGAt                    >  1:1636569/1‑151 (MQ=255)
                                                                                                                                    tACCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGAtc                   >  2:10818172/1‑151 (MQ=255)
                                                                                                                                     aCCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGAtct                  >  1:8773773/1‑151 (MQ=255)
                                                                                                                                     aCCAGGGACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGAtct                  >  2:2536478/1‑151 (MQ=255)
                                                                                                                                          ggACTGCGCGCCTA‑CCTGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGaaa             <  2:4457080/151‑1 (MQ=255)
                                                                                                                                          ggACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGaaa             <  1:5658545/151‑1 (MQ=255)
                                                                                                                                          ggACTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGaaa             >  2:8369760/1‑151 (MQ=255)
                                                                                                                                            aCTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGAAAGt           >  1:6827424/1‑151 (MQ=255)
                                                                                                                                             cTGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGAAAGTc          >  1:9489488/1‑151 (MQ=255)
                                                                                                                                              tGCGCGCCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGAAAGTCg         >  1:3992805/1‑151 (MQ=255)
                                                                                                                                               gcgcgcCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGAAAGTCGc        >  2:8069866/1‑151 (MQ=255)
                                                                                                                                               gcgcgcCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGAAAGTCGc        <  2:2359620/151‑1 (MQ=255)
                                                                                                                                               gcgcgcCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGAAAGTCGc        <  2:9708706/151‑1 (MQ=255)
                                                                                                                                                cgcgcCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGAATGTCGcc       >  2:1509182/1‑151 (MQ=255)
                                                                                                                                                 gcgcCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGAAAGTCGCCt      >  1:5113422/1‑151 (MQ=255)
                                                                                                                                                   gcCTA‑CCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGAAAGTCGCCTgg    >  2:4897380/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                    
GAGCTGGGTCGACCTGCGCCCAGCAGGCGCTGGTCTACCCTCGGCCGCTGGCCGGCGAGCCCGTTCCTCGCGCCGGGCGCGGAGGATGCCGAGGACCAGGGGCGCGCGCTGGCGAGCACGGGCGTCGACGACTACCAGGGACTGCGCGCCTACCCAGCCGGGCGACTCGCGGCGGCGCCTGCACTGGAAGGCGTTTTCCCGTGGCCAGGGGCTGCTGGTGAAGGACTTCGCCGCGCTCGGCGGGGAAGACCTGTGGCTGGAATTCGAGCGCCTCGGCGGGGATCTCGAAAGTCGCCTGGGC  >  NC_008463/2341550‑2341850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑