Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,955,433 A→G A288A (GCT→GCC)  PA14_RS13700 ← PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,955,4330AG99.8% 1934.0 / NA 483A288A (GCT→GCC) PA14_RS13700PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (280/202);  minor base T (1/0);  total (281/202)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 483 total aligned reads displayed.

CGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCG  >  NC_008463/2955285‑2955580
                                                                                                                                                    |                                                                                                                                                   
  ccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCa                                                                                                                                                 >  1:5296609/1‑151 (MQ=255)
    gAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACg                                                                                                                                               >  1:608569/1‑151 (MQ=255)
    gAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACg                                                                                                                                               <  2:2426537/151‑1 (MQ=255)
    gAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACg                                                                                                                                               >  2:6012718/1‑151 (MQ=255)
    gAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACg                                                                                                                                               >  1:10056894/1‑151 (MQ=255)
    gAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGGATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACg                                                                                                                                               >  1:4950448/1‑151 (MQ=255)
      aaTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGaa                                                                                                                                             <  1:4011336/151‑1 (MQ=255)
        tCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGTGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACa                                                                                                                                           <  2:1200905/151‑1 (MQ=255)
          gCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATc                                                                                                                                         >  2:6428260/1‑151 (MQ=255)
          gCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATc                                                                                                                                         <  2:2496936/151‑1 (MQ=255)
             ggTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCggg                                                                                                                                      >  1:3743566/1‑151 (MQ=255)
              gtgtGCAGGGGACGCATGGATTCGGTGACGACCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGc                                                                                                                                     >  2:7602688/1‑151 (MQ=255)
              gtgtGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGc                                                                                                                                     >  1:6817541/1‑151 (MQ=255)
               tgtgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCt                                                                                                                                    <  2:7861816/151‑1 (MQ=255)
                 tgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGt                                                                                                                                  >  1:8510852/1‑151 (MQ=255)
                   cAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAg                                                                                                                                >  1:7035531/1‑151 (MQ=255)
                     ggggACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGGTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGc                                                                                                                              >  1:4504298/1‑151 (MQ=255)
                        gACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGtt                                                                                                                           <  1:9857305/151‑1 (MQ=255)
                        gACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGtt                                                                                                                           <  1:7844265/151‑1 (MQ=255)
                        gACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGtt                                                                                                                           <  2:300812/151‑1 (MQ=255)
                         aCGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTg                                                                                                                          <  1:8706328/151‑1 (MQ=255)
                           gCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGcc                                                                                                                        >  2:10910114/1‑151 (MQ=255)
                           gCAAGGATTCGGTGACGAACAGCAGGGGGGTACCGTCGCAGAGCAGGCGGGTGGTTTCGATCTTTCCGGTATAATGGTTCAGCGCGGCCCAAATGAAAAATATCGGATACCATCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGcc                                                                                                                        >  2:4101013/1‑151 (MQ=255)
                             aTGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCgg                                                                                                                      <  2:8705221/151‑1 (MQ=255)
                               ggATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGc                                                                                                                    >  1:720568/1‑151 (MQ=255)
                                gATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCa                                                                                                                   <  2:7665133/151‑1 (MQ=255)
                                   tCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGa                                                                                                                <  2:4796215/151‑1 (MQ=255)
                                   tCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGa                                                                                                                >  2:9797648/1‑151 (MQ=255)
                                    cGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGac                                                                                                               >  2:6738804/1‑150 (MQ=255)
                                    cGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGaa                                                                                                               <  2:7989574/151‑1 (MQ=255)
                                        gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGGAGGCGTTGCCGGGCAGGAACAcc                                                                                                           >  2:7199578/1‑151 (MQ=255)
                                          cGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgc                                                                                                         <  1:5943897/151‑1 (MQ=255)
                                          cGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgc                                                                                                         <  1:2362173/151‑1 (MQ=255)
                                           gAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgcg                                                                                                        >  2:4935470/1‑151 (MQ=255)
                                             aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCt                                                                                                      >  2:9578640/1‑151 (MQ=255)
                                              cTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTg                                                                                                     <  2:6232122/151‑1 (MQ=255)
                                               tGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTgg                                                                                                    >  2:6782666/1‑151 (MQ=255)
                                                 cAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGcc                                                                                                  >  2:5799357/1‑151 (MQ=255)
                                                   ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATGTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGTGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTGGCCGGGCAGGAACACCGCGCTGGCCTc                                                                                                >  1:5487425/1‑151 (MQ=255)
                                                     ggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCa                                                                                              >  2:7500592/1‑151 (MQ=255)
                                                         tACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCt                                                                                          <  2:2702480/151‑1 (MQ=255)
                                                          aCGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTg                                                                                         >  1:9238040/1‑151 (MQ=255)
                                                           cGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTgg                                                                                        >  2:2195664/1‑151 (MQ=255)
                                                            ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg                                                                                       <  2:5120661/151‑1 (MQ=255)
                                                            ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg                                                                                       >  1:4253653/1‑151 (MQ=255)
                                                            ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg                                                                                       <  1:1541414/151‑1 (MQ=255)
                                                              tCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGa                                                                                     >  1:2565759/1‑151 (MQ=255)
                                                               cGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGAt                                                                                    <  1:9012931/151‑1 (MQ=255)
                                                               cGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGAt                                                                                    <  2:4660785/151‑1 (MQ=255)
                                                               cGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGAt                                                                                    <  2:8520198/151‑1 (MQ=255)
                                                                gCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATa                                                                                   >  2:8961449/1‑151 (MQ=255)
                                                                gCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATa                                                                                   <  2:8905100/151‑1 (MQ=255)
                                                                  tGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACg                                                                                 >  2:1431556/1‑151 (MQ=255)
                                                                        ggCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGt                                                                           <  2:10740049/150‑1 (MQ=255)
                                                                        ggCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGt                                                                           <  2:5587558/150‑1 (MQ=255)
                                                                         gCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTg                                                                          <  1:2993704/151‑1 (MQ=255)
                                                                         gCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTg                                                                          <  2:5281134/151‑1 (MQ=255)
                                                                          cGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGa                                                                         >  1:9144828/1‑151 (MQ=255)
                                                                          cGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGa                                                                         >  1:7805481/1‑151 (MQ=255)
                                                                                gtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGcc                                                                   >  2:10092209/1‑151 (MQ=255)
                                                                                 tttCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCg                                                                  >  1:10304937/1‑151 (MQ=255)
                                                                                 tttCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCg                                                                  <  1:1942869/151‑1 (MQ=255)
                                                                                 tttCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCg                                                                  <  1:3533589/151‑1 (MQ=255)
                                                                                    cGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGTGCAGTAGGCGTTGCCGGGCAGGAACACCGCGCTTGCCTCCAGGCTGGGGATACGCCCGCTGACCAGCCGCAc                                                               >  2:11013822/1‑151 (MQ=255)
                                                                                     gATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCAcc                                                              >  2:10365652/1‑151 (MQ=255)
                                                                                     gATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCAcc                                                              >  1:8066518/1‑151 (MQ=255)
                                                                                     gATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCAcc                                                              >  1:9699436/1‑151 (MQ=255)
                                                                                      aTCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCg                                                             >  1:5815648/1‑151 (MQ=255)
                                                                                         ttGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCt                                                          <  1:1725428/151‑1 (MQ=255)
                                                                                              ggTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTcc                                                     >  2:9273285/1‑151 (MQ=255)
                                                                                                   aaGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTc                                                >  1:6361451/1‑151 (MQ=255)
                                                                                                      ggTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGAt                                             <  1:8238996/151‑1 (MQ=255)
                                                                                                        ttCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGt                                           >  2:6163681/1‑151 (MQ=255)
                                                                                                         tCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTa                                          <  1:10929157/151‑1 (MQ=255)
                                                                                                              gcgGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGaa                                     <  1:1273495/151‑1 (MQ=255)
                                                                                                              gcgGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGaa                                     >  2:3458009/1‑151 (MQ=255)
                                                                                                              gcgGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGaa                                     >  1:7393159/1‑151 (MQ=255)
                                                                                                               cgGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAAc                                    >  2:4302001/1‑151 (MQ=255)
                                                                                                                ggCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAAcc                                   >  1:6499698/1‑151 (MQ=255)
                                                                                                                   cGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTc                                >  1:2799956/1‑151 (MQ=255)
                                                                                                                    gACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCt                               >  1:3856415/1‑151 (MQ=255)
                                                                                                                    gACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCt                               >  2:3794446/1‑151 (MQ=255)
                                                                                                                      cTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGa                             >  2:10627733/1‑151 (MQ=255)
                                                                                                                       tGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGAc                            >  1:5143703/1‑151 (MQ=255)
                                                                                                                         gAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCg                          >  2:9427718/1‑151 (MQ=255)
                                                                                                                          aaCATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCgg                         <  2:708133/151‑1 (MQ=255)
                                                                                                                            cATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGt                       >  1:4576648/1‑151 (MQ=255)
                                                                                                                            cATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGt                       <  1:9998127/151‑1 (MQ=255)
                                                                                                                             aTGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCGGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTg                      >  2:2782765/1‑151 (MQ=255)
                                                                                                                                aGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcgg                   <  1:7480712/151‑1 (MQ=255)
                                                                                                                                      tACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCggg             >  1:8936737/1‑151 (MQ=255)
                                                                                                                                       aCCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCgggg            <  1:7686016/151‑1 (MQ=255)
                                                                                                                                        ccAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGc           <  1:10187778/151‑1 (MQ=255)
                                                                                                                                        ccAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGc           >  1:9514757/1‑151 (MQ=255)
                                                                                                                                         cAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCa          >  2:11139050/1‑151 (MQ=255)
                                                                                                                                          aGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGGTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCAc         <  2:8324518/151‑1 (MQ=255)
                                                                                                                                            ccGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGt       <  1:3308363/151‑1 (MQ=255)
                                                                                                                                             cgcgTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTc      <  2:1342012/151‑1 (MQ=255)
                                                                                                                                               cgTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCgg    >  1:10671285/1‑151 (MQ=255)
                                                                                                                                                gTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGACCCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgc   >  2:156406/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCG  >  NC_008463/2955285‑2955580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑