Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,051,401 T→C N373D (AAC→GAC)  PA14_RS13990 ← SfnB family sulfur acquisition oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,051,4010TC100.0% 1959.9 / NA 486N373D (AAC→GAC) PA14_RS13990SfnB family sulfur acquisition oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (272/214);  total (272/214)

Only 100 of 512 total aligned reads displayed.

TAGCGTGTGCGAGGCTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTTGAGGTTGAACTTGGCGAGCGTGGCGCGGCTGCCGGCCAGCTTGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGC  >  NC_008463/3051253‑3051548
                                                                                                                                                    |                                                                                                                                                   
       tgCGAGGCTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGaa                                                                                                                                            <  2:4867702/151‑1 (MQ=255)
          gAGGCTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACtc                                                                                                                                         >  1:7424095/1‑150 (MQ=255)
          gAGGCTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACtc                                                                                                                                         <  1:8698846/151‑2 (MQ=255)
          gAGGCTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTGCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGAGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGGTGAACtc                                                                                                                                         >  2:3036554/1‑150 (MQ=255)
             gCTCATCGGGCGCTCCTCAGATCCATGAGTGCCGCGCCGGACGGGCGCCGTTCAGGTAGGAGTCGCCGACCGCGGGGACCTTCCAGCGTACCGGGACGGGCAGGGTGGGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGc                                                                                                                                      <  2:2357093/151‑1 (MQ=255)
             gCTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGc                                                                                                                                      <  1:9598795/151‑1 (MQ=255)
              cTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCg                                                                                                                                     >  2:7175405/1‑151 (MQ=255)
                 aTCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGc                                                                                                                                  <  2:8616761/151‑1 (MQ=255)
                       cgcTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGgcg                                                                                                                            <  1:10167734/151‑1 (MQ=255)
                        gcTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGgcgc                                                                                                                           >  1:8208796/1‑151 (MQ=255)
                        gcTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGgcgc                                                                                                                           >  2:8881047/1‑151 (MQ=255)
                        gcTCCGCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGGGGAGGGTGTGGGGGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGgcgc                                                                                                                           >  2:5315765/1‑151 (MQ=37)
                          tcctcAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCgg                                                                                                                         <  1:1371558/151‑1 (MQ=255)
                            ctcAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCTTGGCGCGGca                                                                                                                       >  1:3859872/1‑150 (MQ=255)
                            ctcAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCt                                                                                                                       <  2:4473999/151‑1 (MQ=255)
                            ctcAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCt                                                                                                                       <  1:11141803/151‑1 (MQ=255)
                              cAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGc                                                                                                                     >  2:5854402/1‑151 (MQ=255)
                                gATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCg                                                                                                                   >  1:3494207/1‑151 (MQ=255)
                                 aTCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCgg                                                                                                                  <  1:2645518/151‑1 (MQ=255)
                                    cATGAGTGACGCGCCGGACGGGCGCCGTTCCGGTAGTAGTCGCCGACCGCGGGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCa                                                                                                               >  1:4737106/1‑151 (MQ=255)
                                       gAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCt                                                                                                            <  2:605939/151‑1 (MQ=255)
                                        aGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCtc                                                                                                           <  1:4574148/151‑2 (MQ=255)
                                          tGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGa                                                                                                         >  1:5232628/1‑151 (MQ=255)
                                            aCGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGCCCGCGTGGACCTTCCAGCGGACCGGGTCGTGCAGGGTGTGGGGGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAag                                                                                                       <  2:2841047/151‑1 (MQ=255)
                                            aCGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAag                                                                                                       <  2:10713234/151‑1 (MQ=255)
                                             cgcgCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAaga                                                                                                      <  1:10328599/151‑1 (MQ=255)
                                              gcgcCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAagag                                                                                                     <  1:1507773/151‑1 (MQ=255)
                                                   ggACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTtct                                                                                                >  1:8361934/1‑151 (MQ=255)
                                                    gACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTtctc                                                                                               >  1:8550537/1‑151 (MQ=255)
                                                       gggCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCt                                                                                            >  2:7522714/1‑151 (MQ=255)
                                                        ggCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTg                                                                                           <  2:1454449/151‑1 (MQ=255)
                                                        ggCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTg                                                                                           <  1:6817917/151‑1 (MQ=255)
                                                           gcCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTggcg                                                                                        <  2:4281592/151‑1 (MQ=255)
                                                            ccGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTggcgg                                                                                       <  2:5772216/151‑1 (MQ=255)
                                                             cGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTggcggc                                                                                      <  1:2978837/151‑1 (MQ=255)
                                                             cGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTggcggc                                                                                      >  2:8108135/1‑151 (MQ=255)
                                                              gTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGcc                                                                                     >  1:4477379/1‑151 (MQ=255)
                                                              gTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGcc                                                                                     <  2:8033457/151‑1 (MQ=255)
                                                               ttCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCa                                                                                    >  2:5524246/1‑151 (MQ=255)
                                                               ttCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCa                                                                                    >  2:6760276/1‑151 (MQ=255)
                                                                 cAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAgg                                                                                  <  2:844850/151‑1 (MQ=255)
                                                                 cAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAgg                                                                                  <  2:6846870/151‑1 (MQ=255)
                                                                 cAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAgg                                                                                  >  2:965328/1‑151 (MQ=255)
                                                                 cAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAgg                                                                                  <  2:1966377/151‑1 (MQ=255)
                                                                  aGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGc                                                                                 <  2:2174503/151‑1 (MQ=255)
                                                                   ggTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCt                                                                                <  2:8700747/151‑1 (MQ=255)
                                                                     tagtagTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGa                                                                              >  1:6620906/1‑151 (MQ=255)
                                                                        tagtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGAtct                                                                           >  2:3792138/1‑151 (MQ=255)
                                                                        tagtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGAtct                                                                           <  1:3948128/151‑1 (MQ=255)
                                                                          gtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCg                                                                         >  2:349951/1‑151 (MQ=255)
                                                                          gtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCg                                                                         >  2:9736025/1‑151 (MQ=255)
                                                                             gCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTg                                                                      >  1:6059743/1‑151 (MQ=255)
                                                                             gCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTg                                                                      >  2:4669267/1‑151 (MQ=255)
                                                                              ccGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGc                                                                     >  1:979465/1‑151 (MQ=255)
                                                                               cGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCt                                                                    >  2:2594543/1‑151 (MQ=255)
                                                                                gACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTc                                                                   >  1:5325907/1‑151 (MQ=255)
                                                                                 aCCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCa                                                                  <  1:10817715/151‑1 (MQ=255)
                                                                                 aCCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCa                                                                  <  1:7794387/151‑1 (MQ=255)
                                                                                    gcgTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCa                                                               >  1:2351873/1‑151 (MQ=255)
                                                                                      gTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCAcc                                                             >  1:4999797/1‑151 (MQ=255)
                                                                                      gTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCAcc                                                             >  1:8961600/1‑151 (MQ=255)
                                                                                        ggACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCtt                                                           >  2:6111415/1‑151 (MQ=255)
                                                                                        ggACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCtt                                                           <  1:9202138/151‑1 (MQ=255)
                                                                                         gACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTg                                                          <  2:1126992/151‑1 (MQ=255)
                                                                                         gACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTg                                                          <  2:6311084/151‑1 (MQ=255)
                                                                                         gACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTg                                                          <  1:6241339/151‑1 (MQ=255)
                                                                                            cTTCCAGCGTCCCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGcc                                                       <  2:10529255/151‑1 (MQ=255)
                                                                                            cTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGACAGCTCTAAGAGCTTCTCGCTGGCGGCCAGGATGATCTCGGTGCTCAGCACCTTGGcc                                                       <  2:7363553/151‑1 (MQ=38)
                                                                                                   cGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCa                                                >  1:5779551/1‑151 (MQ=255)
                                                                                                    gTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCAc                                               >  2:9099922/1‑151 (MQ=255)
                                                                                                     tACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACg                                              >  2:6506058/1‑151 (MQ=255)
                                                                                                      tccGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACgg                                             <  1:5283845/150‑1 (MQ=255)
                                                                                                      aCCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACgg                                             >  2:450819/1‑151 (MQ=255)
                                                                                                       ccGGGTCGTGCAGGGAGTGGGGGCGGGCGTTGCGCCCGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCATGGCCTCGGCCACGGc                                            <  2:1761763/151‑1 (MQ=255)
                                                                                                        cGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTAGTCCTCGGCCACGGCg                                           >  1:4391188/1‑151 (MQ=255)
                                                                                                           gTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCGCAGCACCTTGGCCTCGGCCACGGCGATc                                        >  1:6331243/1‑151 (MQ=255)
                                                                                                             cGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGa                                      >  1:9139007/1‑151 (MQ=255)
                                                                                                              gTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAg                                     >  1:1950263/1‑151 (MQ=255)
                                                                                                              gTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAg                                     >  1:280646/1‑151 (MQ=255)
                                                                                                                gCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGgc                                   <  2:7714106/151‑1 (MQ=255)
                                                                                                                  aGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGgcgc                                 >  2:4075181/1‑151 (MQ=255)
                                                                                                                      tgtgGGTGCGGGCGGTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTgc                             <  2:3233322/151‑1 (MQ=255)
                                                                                                                           gTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGccgccg                        >  1:1209054/1‑151 (MQ=255)
                                                                                                                            tGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGa                       <  1:6531632/151‑1 (MQ=255)
                                                                                                                             gCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGCGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGAc                      <  2:951623/151‑1 (MQ=255)
                                                                                                                                  cGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGc                 <  2:1284174/151‑1 (MQ=255)
                                                                                                                                  cGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGc                 <  2:10972057/151‑1 (MQ=255)
                                                                                                                                  cGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGc                 <  1:7718109/151‑1 (MQ=255)
                                                                                                                                  cGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGc                 <  1:7232612/151‑1 (MQ=255)
                                                                                                                                   gTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTCCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCg                >  2:10080137/1‑151 (MQ=255)
                                                                                                                                    ttGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGt               <  1:9342003/151‑1 (MQ=255)
                                                                                                                                      gcgcCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTcc             >  1:4851514/1‑151 (MQ=255)
                                                                                                                                       cgcCAGTGCCGGTCGCGGTTGCACTCGGCGCGCGGGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCGGATCTCGGTGCTCAGCACCTTGGCCTCCGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCa            <  2:6603115/151‑1 (MQ=255)
                                                                                                                                       cgcCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCa            <  1:3703832/151‑1 (MQ=255)
                                                                                                                                         ccAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCAcc          >  1:10004273/1‑151 (MQ=255)
                                                                                                                                           aGTGCCGGTCGAGGTTGAACTCGTCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGTGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCgg        >  1:3998164/1‑151 (MQ=255)
                                                                                                                                           aGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCgg        >  1:5528237/1‑151 (MQ=255)
                                                                                                                                              gCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcg     >  1:1306938/1‑151 (MQ=255)
                                                                                                                                              gCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcg     <  1:3303405/151‑1 (MQ=255)
                                                                                                                                                 ggtcgaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcgcgc  >  2:6926737/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TAGCGTGTGCGAGGCTCATCGGGCGCTCCTCAGATCCATGAGTGACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTTGAGGTTGAACTTGGCGAGCGTGGCGCGGCTGCCGGCCAGCTTGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGC  >  NC_008463/3051253‑3051548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑