Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,373,667 G→C V352V (GTC→GTG)  PA14_RS15385 ← aminotransferase class V‑fold PLP‑dependent enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,373,6670GC99.8% 2025.9 / NA 505V352V (GTC→GTG) PA14_RS15385aminotransferase class V‑fold PLP‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (251/253);  minor base A (0/1);  total (251/254)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 507 total aligned reads displayed.

GAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGGACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGTC  >  NC_008463/3373519‑3373814
                                                                                                                                                    |                                                                                                                                                   
gAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCAc                                                                                                                                                   >  2:2733956/1‑151 (MQ=255)
gAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCAc                                                                                                                                                   >  1:8404483/1‑151 (MQ=255)
gAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCAc                                                                                                                                                   >  1:2773912/1‑151 (MQ=255)
gAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCAc                                                                                                                                                   <  2:9866092/151‑1 (MQ=255)
gAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCAc                                                                                                                                                   >  1:1565080/1‑151 (MQ=255)
  gggCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCCGGCGCACGc                                                                                                                                                 >  1:1308602/1‑151 (MQ=255)
     cGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGc                                                                                                                                              <  1:5762872/151‑1 (MQ=255)
          cGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCa                                                                                                                                         <  1:6040939/151‑1 (MQ=255)
          cGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCa                                                                                                                                         <  2:9714315/151‑1 (MQ=255)
               cggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCt                                                                                                                                    >  1:375206/1‑151 (MQ=255)
               cggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCt                                                                                                                                    >  1:9803713/1‑151 (MQ=255)
               cggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCt                                                                                                                                    >  2:9171947/1‑151 (MQ=255)
                      tCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCa                                                                                                                             <  2:6439670/151‑1 (MQ=255)
                      tCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCa                                                                                                                             >  2:1942623/1‑151 (MQ=255)
                      tCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCa                                                                                                                             >  2:685684/1‑151 (MQ=255)
                        aGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGAAGAGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATc                                                                                                                           >  2:5460039/1‑151 (MQ=255)
                          ccTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGt                                                                                                                         >  1:9169344/1‑151 (MQ=255)
                          ccTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGt                                                                                                                         >  1:9591681/1‑151 (MQ=255)
                           cTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTg                                                                                                                        >  2:10212191/1‑151 (MQ=255)
                           cTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTg                                                                                                                        >  1:2262469/1‑151 (MQ=255)
                           cTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTg                                                                                                                        >  2:3468273/1‑151 (MQ=255)
                               ccTGGCTACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCg                                                                                                                    <  1:5357493/151‑1 (MQ=255)
                                  ggATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAgg                                                                                                                 >  1:3049138/1‑151 (MQ=255)
                                  ggATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAgg                                                                                                                 >  2:570074/1‑151 (MQ=255)
                                    aTACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGc                                                                                                               <  1:1468314/151‑1 (MQ=255)
                                     tACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCg                                                                                                              >  1:11002325/1‑151 (MQ=255)
                                       ccccGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCgag                                                                                                            <  2:907056/151‑1 (MQ=255)
                                        cccGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCgaga                                                                                                           <  1:8852623/151‑1 (MQ=255)
                                         ccGAGGAACGGCTGCCACGCCGGCGCGCCACTCTCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGaa                                                                                                          <  1:10906425/151‑1 (MQ=255)
                                         ccGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGaa                                                                                                          >  2:2424326/1‑151 (MQ=255)
                                         ccGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGaa                                                                                                          <  2:9487283/151‑1 (MQ=255)
                                          cGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAg                                                                                                         >  2:132577/1‑151 (MQ=255)
                                          cGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAg                                                                                                         <  2:10929488/151‑1 (MQ=255)
                                            aGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGcc                                                                                                       >  1:8421993/1‑150 (MQ=255)
                                            aGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCa                                                                                                       >  2:8305026/1‑151 (MQ=255)
                                                  ggCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTggg                                                                                                 >  1:4542154/1‑151 (MQ=255)
                                                  ggCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTggg                                                                                                 >  2:176286/1‑151 (MQ=255)
                                                   gCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGa                                                                                                <  2:7018165/151‑1 (MQ=255)
                                                   gCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGa                                                                                                >  2:4691137/1‑151 (MQ=255)
                                                   gCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGa                                                                                                <  2:10104423/151‑1 (MQ=255)
                                                      gCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACgg                                                                                             >  1:5843972/1‑151 (MQ=255)
                                                       ccACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGgc                                                                                            >  1:3293018/1‑151 (MQ=255)
                                                        cACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGgcg                                                                                           >  2:618703/1‑151 (MQ=255)
                                                          cGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgcc                                                                                         <  1:10462231/151‑1 (MQ=255)
                                                            ccGGCGCGCCACTCTCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgccgg                                                                                       <  2:4841909/151‑1 (MQ=255)
                                                                  cgcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCgcgc                                                                                 >  1:407791/1‑151 (MQ=255)
                                                                   gcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGcc                                                                                >  1:10094439/1‑151 (MQ=255)
                                                                     cACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGa                                                                              >  2:262245/1‑151 (MQ=255)
                                                                     cACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGa                                                                              <  1:437042/151‑1 (MQ=255)
                                                                        tATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGtt                                                                           <  2:7273975/151‑1 (MQ=255)
                                                                         aTCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTg                                                                          >  2:6523411/1‑151 (MQ=255)
                                                                         aTCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTg                                                                          >  1:706198/1‑151 (MQ=255)
                                                                         aTCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTg                                                                          <  1:6314000/151‑1 (MQ=255)
                                                                            cgccgcGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAg                                                                       <  1:4839199/151‑1 (MQ=255)
                                                                             gccgcGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAgg                                                                      >  2:5303856/1‑151 (MQ=255)
                                                                              ccgcGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGc                                                                     <  2:7962066/151‑1 (MQ=255)
                                                                              ccgcGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGc                                                                     <  2:8156252/151‑1 (MQ=255)
                                                                               cgcgGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGcc                                                                    <  2:4128987/151‑1 (MQ=255)
                                                                                gcgGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCg                                                                   <  2:1165665/151‑1 (MQ=255)
                                                                                gcgGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCg                                                                   <  1:3484051/151‑1 (MQ=255)
                                                                                   gCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAgg                                                                <  2:5892685/151‑1 (MQ=255)
                                                                                   gCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAgg                                                                <  1:6584797/151‑1 (MQ=255)
                                                                                    ccTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGt                                                               <  2:4344618/151‑1 (MQ=255)
                                                                                      ttGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGc                                                             >  2:3923042/1‑151 (MQ=255)
                                                                                       tGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCt                                                            >  2:7614292/1‑151 (MQ=255)
                                                                                        gAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTg                                                           <  1:1724463/151‑1 (MQ=255)
                                                                                        gAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTCGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTg                                                           >  1:10676829/1‑151 (MQ=255)
                                                                                                ccACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGc                                                   >  1:9472290/1‑151 (MQ=255)
                                                                                                  aCCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCa                                                 <  1:7823211/151‑1 (MQ=255)
                                                                                                   ccgccgCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAg                                                <  1:298936/151‑1 (MQ=255)
                                                                                                   ccgccgCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAg                                                <  2:3739335/151‑1 (MQ=255)
                                                                                                    cgccgcGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAgg                                               >  1:7548755/1‑151 (MQ=255)
                                                                                                      ccgcGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCt                                             <  1:10288794/151‑1 (MQ=255)
                                                                                                           tCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACt                                        >  2:5864752/1‑151 (MQ=255)
                                                                                                           tCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACt                                        <  1:9954146/151‑1 (MQ=255)
                                                                                                             gACGTCGGCCTCGCGGGTGTCGCGACCGAGGCTCAGGCGCACGCGGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTcc                                      >  1:254430/1‑151 (MQ=255)
                                                                                                               cGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAg                                    >  2:836336/1‑151 (MQ=255)
                                                                                                                gTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGt                                   <  1:9338996/151‑1 (MQ=255)
                                                                                                                gTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGt                                   <  1:5101854/151‑1 (MQ=255)
                                                                                                                 tCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTg                                  >  1:5287273/1‑151 (MQ=255)
                                                                                                                  cGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGc                                 <  2:10640809/151‑1 (MQ=255)
                                                                                                                   ggCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGcc                                >  2:7705113/1‑151 (MQ=255)
                                                                                                                    gCCTCGCGGGTGTAGCGACCGAGGCTCAGTCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGCGACGGCGCCGGCCGCGCCGAGTAGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCt                               >  2:7427742/1‑151 (MQ=255)
                                                                                                                      cTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTgg                             <  1:6807532/151‑1 (MQ=255)
                                                                                                                          cgGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGa                         >  2:4544124/1‑151 (MQ=255)
                                                                                                                           gggTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGAc                        >  1:9137275/1‑151 (MQ=255)
                                                                                                                             gtgtAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACaa                      >  2:2079542/1‑151 (MQ=255)
                                                                                                                                tAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAgg                   >  2:11142266/1‑151 (MQ=255)
                                                                                                                                 atcGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGTCACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGc                  <  2:4446066/149‑1 (MQ=255)
                                                                                                                                 aGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGc                  >  1:7474101/1‑151 (MQ=255)
                                                                                                                                   cGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCg                >  2:3198580/1‑151 (MQ=255)
                                                                                                                                    gACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCgg               >  1:2135520/1‑151 (MQ=255)
                                                                                                                                      ccGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGt             <  1:6427174/151‑1 (MQ=255)
                                                                                                                                        gAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTg           >  1:5001947/1‑151 (MQ=255)
                                                                                                                                        gAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTg           >  1:2680715/1‑151 (MQ=255)
                                                                                                                                        gAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGAGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTg           <  2:7163907/151‑1 (MQ=255)
                                                                                                                                          ggCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTc         <  2:309651/151‑1 (MQ=255)
                                                                                                                                             tCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGaa      >  1:200135/1‑151 (MQ=255)
                                                                                                                                             tCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGaa      <  1:11172115/151‑1 (MQ=255)
                                                                                                                                                 gcgcACGCTGCGGCCAGCGCTGGCGGCATCGGGGCCGAGGGCGAGAAGCACGGGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGGGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGTc  <  2:8781472/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGGACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGTC  >  NC_008463/3373519‑3373814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑