Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,423,281 A→G K130K (AAA→AAG)  mexY → multidrug efflux RND transporter permease subunit MexY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,423,2810AG98.3% 1697.0 / ‑5.1 526K130K (AAA→AAG) mexYmultidrug efflux RND transporter permease subunit MexY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (249/268);  minor base T (9/0);  total (258/268)
Fisher's exact test for biased strand distribution p-value = 1.53e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 531 total aligned reads displayed.

CCAGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAAGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCCAGACCTGGGGCGCC  >  NC_008463/3423133‑3423428
                                                                                                                                                    |                                                                                                                                                   
ccaGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgc                                                                                                                                                   <  2:5207683/151‑1 (MQ=255)
ccaGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgc                                                                                                                                                   >  2:3487961/1‑151 (MQ=255)
ccaGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGTGCGGCGCGACGGTATCTAAGTGGAGAAGgc                                                                                                                                                   >  1:2393497/1‑151 (MQ=255)
    cagcaCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgcggcg                                                                                                                                               <  1:4509836/151‑1 (MQ=255)
      gcaCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGGCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGa                                                                                                                                             >  2:4681902/1‑151 (MQ=255)
        aCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCGGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGTCGGTATCTCCGGGGAGAAGGCGGCGGACa                                                                                                                                           >  2:7528504/1‑151 (MQ=255)
          cGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGGCGGTGGGGCGCGTCGGGATCTACGTGGTGAAGGCGGCGGACAGc                                                                                                                                         >  1:1046781/1‑151 (MQ=255)
           ggCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCa                                                                                                                                        >  1:3122886/1‑151 (MQ=255)
           ggCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCCGGGCGGGGCGACGGTCTGAACGGGGAGCATGAGGCCGACAGcc                                                                                                                                        >  1:8982740/1‑150 (MQ=255)
              cAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGGCGGTATCTACGTGGAGTAGGCGGCGGACAGCATcc                                                                                                                                     >  1:2282336/1‑151 (MQ=255)
              cAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATATACGTGGAGAAGGCGGCGGACAGCATcc                                                                                                                                     >  1:5651429/1‑151 (MQ=255)
               aGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCa                                                                                                                                    >  2:10416558/1‑151 (MQ=255)
                ggCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGGCCGAGTCGGGGCGGCGCGGCGGTCTCTACGGGGAGAAGGCGGGGGTCAGCCTCCAg                                                                                                                                   >  1:7662378/1‑151 (MQ=255)
                ggCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAg                                                                                                                                   <  1:4602322/151‑1 (MQ=255)
                 gCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGc                                                                                                                                  <  2:1097460/151‑1 (MQ=255)
                   tacGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCtc                                                                                                                                <  1:7335400/149‑2 (MQ=255)
                    tCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGa                                                                                                                               <  2:10771055/151‑1 (MQ=255)
                     cGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGGGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACCGCAACCAGCTGAt                                                                                                                              >  2:4825146/1‑151 (MQ=255)
                     cGCTGACCCGGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCCGAACCGCCTGAAGCTCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTgct                                                                                                                              <  2:2227016/151‑3 (MQ=255)
                          accctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCa                                                                                                                         <  2:11052805/151‑1 (MQ=255)
                             ctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGGATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGTTCGTCAccc                                                                                                                      >  2:7667453/1‑151 (MQ=255)
                                accTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCa                                                                                                                   <  2:4512837/151‑1 (MQ=255)
                                 ccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGGGCGGCGCGACGGTATCTACGTGGAGTAGGCGGCGGACAGCATCCAGCTGATCGTCACCCacac                                                                                                                  >  1:10348677/1‑151 (MQ=255)
                                      cGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGAACGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGTCGGGATCTAAGTGGAGAATGCGGCGGACAGCATCCAGATGATCGTCACCCTCAcctcct                                                                                                             >  2:8191239/1‑151 (MQ=255)
                                        ccAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCCGCAGATCGTCACCCTCCCCtcctcc                                                                                                           >  2:9408513/1‑151 (MQ=255)
                                         cAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCa                                                                                                          <  1:2146342/151‑1 (MQ=255)
                                         cAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCa                                                                                                          <  2:7795069/151‑1 (MQ=255)
                                          aGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGCCGGTATCTACGTGGAGAATGCGGCGGACAGCAACCAGCTGATCGTCACCCACACCACCTCCac                                                                                                         >  1:4662983/1‑150 (MQ=255)
                                          aGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGTGAAGGCGGCGGAACGCATCCAGCTGTTCGTCACCCTCACCTCCACCAg                                                                                                         >  2:1750883/1‑151 (MQ=255)
                                          aGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGTGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAg                                                                                                         >  2:1404211/1‑151 (MQ=255)
                                               gTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTAGCGCCAGCCCGAGTCGGTGCGGCGCGACGGTATCTAAGTGGGGAAGGCGGCGGACAGCATCCAGCTGATCGGCACCCTCACCTCCTCCAGCGGcc                                                                                                    >  1:9787041/1‑151 (MQ=255)
                                                 gAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCGCCAGCGGCCGc                                                                                                  >  2:6236501/1‑151 (MQ=255)
                                                   aCGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGTCGGTATATACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCACCTCCAGCGGCCGCTa                                                                                                >  2:9278797/1‑151 (MQ=255)
                                                    cgccgcCCTCGCCGCGGTGGAAGAGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTac                                                                                               <  2:7896108/151‑1 (MQ=255)
                                                    cGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTac                                                                                               <  2:3321584/151‑1 (MQ=255)
                                                     gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTGGCGCCTGCCCGAGTCGGGGCGGCGCGGCGGTATCTACGTGGTGAAGGCGGCGGGCAGCATACAGCTGATAGTCACCCGCACCTCCTACACCGGCCGCTacg                                                                                              >  1:8393910/1‑151 (MQ=255)
                                                      ccGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCAGCCCGAGTCGGTGCGGCGCGACGGTTTCTACGTGGAGAATGCGGCGGACAGCATACAGCTGATCGTCACCCTCACCTCCACCAGCCGCCGCTacga                                                                                             >  1:5010321/1‑151 (MQ=255)
                                                       cGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacgac                                                                                            <  1:6857539/151‑1 (MQ=255)
                                                        gACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGGGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCACACCTCCTCCCGCGGCCGCGacgacg                                                                                           >  1:3338841/1‑151 (MQ=255)
                                                            tCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCCGCGGCCGCTACGACGCCAt                                                                                       >  2:8844348/1‑151 (MQ=255)
                                                             cgccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGCCAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATg                                                                                      <  1:5319934/151‑1 (MQ=255)
                                                             cgccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATg                                                                                      <  1:9520429/151‑1 (MQ=255)
                                                             cgccgcGGGGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGAGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACAGCATCCAGCGGATCGTCACCCTCACCTCCTCCAGCGGCCGCGACGACGCCATg                                                                                      <  2:5685398/151‑1 (MQ=255)
                                                               ccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGCCAGCATACAGCTGATCGTCACCCACACCGCCTCCAGCGGCCGCTACGACGCCATGGa                                                                                    >  2:4098325/1‑151 (MQ=255)
                                                               ccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGGATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCGCCCGCGGCCGCGACGACGCCCTGGa                                                                                    >  1:7829085/1‑151 (MQ=255)
                                                                  cgGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGGAGGTATATACGTGGAGTAGGCGGCGGTCAGCATCCAGCTGATCGTCACCCACACCACCTCCAGCGGCCGCTAAGACGCCAAGGAACt                                                                                 >  1:1159786/1‑151 (MQ=255)
                                                                  cgGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACt                                                                                 <  1:7465021/151‑1 (MQ=255)
                                                                  cgGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGGATCTAAGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCACACCTCCACCAGCGGCCGCTACGACGCCAAGGAACt                                                                                 >  1:5822717/1‑151 (MQ=255)
                                                                     tGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTggg                                                                              <  2:2562040/151‑1 (MQ=255)
                                                                      ggAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGc                                                                             <  2:6375088/151‑1 (MQ=255)
                                                                      ggAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGc                                                                             <  2:5426590/151‑1 (MQ=255)
                                                                      ggAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGc                                                                             <  2:9469784/151‑1 (MQ=255)
                                                                            gCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATc                                                                       <  1:9311512/151‑1 (MQ=255)
                                                                             cAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGGATCTACGGGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCGCCAGCGGCCGCTACGCCGCCATGGAACTGGGCGAGATCg                                                                      >  2:9008085/1‑151 (MQ=255)
                                                                               gAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGcc                                                                    <  1:2766036/151‑1 (MQ=255)
                                                                                 aCCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTc                                                                  <  2:5593954/151‑1 (MQ=255)
                                                                                  ccGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGAAGGTATATAAGTGGAGAAGGCGGCGGAACGCATCCAGCGGATCGGGACCCGCACCACCTCCAGGGGCCGCGACGAAGCCCTGGTACTGGGCGATGTCGCATCg                                                                 >  2:2938672/1‑151 (MQ=255)
                                                                                     ccTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTcc                                                              <  1:7229808/151‑1 (MQ=255)
                                                                                      cTGAAGATCGTCGAGTCGCGCCTGCCCGTGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCAGATCGTCACCCACACCTCCTCCCGCGGCCGCTACGACGCCAAGGAACTGGGCGAGATCGCCTCGTCCa                                                             >  2:9657413/1‑151 (MQ=255)
                                                                                       tGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCaa                                                            <  2:3550014/151‑1 (MQ=255)
                                                                                          aGATCGTCGAGTCGCGCCGGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACgt                                                         <  1:158813/151‑1 (MQ=255)
                                                                                                  gAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAgg                                                 <  1:5914352/151‑1 (MQ=255)
                                                                                                      cgcgCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCt                                             >  2:129165/1‑151 (MQ=255)
                                                                                                      cgcgCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCt                                             <  1:816081/151‑1 (MQ=255)
                                                                                                        cgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTgc                                           <  2:7409554/151‑1 (MQ=255)
                                                                                                           cTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGcc                                        >  1:8621942/1‑151 (MQ=255)
                                                                                                             gCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCgg                                      >  2:675342/1‑151 (MQ=255)
                                                                                                             gCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCgg                                      >  1:10792039/1‑151 (MQ=255)
                                                                                                              cccGAGTCGGTGCGGCGCGACGGTATCTTCGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCggg                                     >  1:4236862/1‑151 (MQ=255)
                                                                                                               ccGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGt                                    <  1:7001734/151‑1 (MQ=255)
                                                                                                                cGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTg                                   <  1:10943868/151‑1 (MQ=255)
                                                                                                                   gTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAg                                <  1:1104025/151‑1 (MQ=255)
                                                                                                                    tCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAgg                               <  1:6452074/151‑1 (MQ=255)
                                                                                                                      ggTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGc                             >  2:855718/1‑151 (MQ=255)
                                                                                                                      ggTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGc                             <  2:3258690/151‑1 (MQ=255)
                                                                                                                        tGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGt                           >  1:3793296/1‑151 (MQ=255)
                                                                                                                        tGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGAGCGTCACCCTCACCTCCTCCAGCGGCGGCTACGACGCCATGGAGCTGGGCGAGAGCGCCTCGTCCAAGGTGTTGCAGGCGCGGCGCCGGGTGGAGGGCGt                           >  2:10171162/1‑151 (MQ=255)
                                                                                                                         gcggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTg                          <  2:1667653/151‑1 (MQ=255)
                                                                                                                           ggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTggg                        >  2:5817424/1‑151 (MQ=255)
                                                                                                                               cgACGGTCTCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCCGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCCAg                    >  2:3021088/1‑151 (MQ=255)
                                                                                                                               cgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAg                    <  2:9598770/151‑1 (MQ=255)
                                                                                                                                gACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAgg                   <  2:4284306/151‑1 (MQ=255)
                                                                                                                                   ggTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTcg                <  2:2288972/151‑2 (MQ=255)
                                                                                                                                   ggTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTcg                <  1:3550923/151‑2 (MQ=255)
                                                                                                                                   ggTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACGGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAgggcg                <  2:734087/151‑4 (MQ=255)
                                                                                                                                    gTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTcga               >  1:6671589/1‑149 (MQ=255)
                                                                                                                                    gTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTcga               <  1:6279/151‑3 (MQ=255)
                                                                                                                                       tCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGAc            <  2:2075301/151‑1 (MQ=255)
                                                                                                                                          aCGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTg         >  2:9162858/1‑151 (MQ=255)
                                                                                                                                          aCGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTg         <  1:6021394/151‑1 (MQ=255)
                                                                                                                                           cGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgg        >  2:3689515/1‑151 (MQ=255)
                                                                                                                                           cGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgg        <  1:4867528/151‑1 (MQ=255)
                                                                                                                                            gTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTggg       <  1:9602399/151‑1 (MQ=255)
                                                                                                                                            gTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTggg       >  2:4631916/1‑151 (MQ=255)
                                                                                                                                             tGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgggg      <  1:4393252/151‑1 (MQ=255)
                                                                                                                                             tGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgggg      <  1:10630600/151‑1 (MQ=255)
                                                                                                                                              ggAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgc     <  1:8082929/151‑1 (MQ=255)
                                                                                                                                                agaAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgcgc   >  1:10015951/1‑151 (MQ=255)
                                                                                                                                                agaAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCGGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgcgc   >  1:990643/1‑151 (MQ=255)
                                                                                                                                                 gaAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCGCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGGCGcc  <  2:10633235/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCAGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAAGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCCAGACCTGGGGCGCC  >  NC_008463/3423133‑3423428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑