Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,565,040 A→G F307F (TTT→TTC)  PA14_RS20885 ← anthranilate synthase component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,565,0400AG99.8% 2510.0 / NA 623F307F (TTT→TTC) PA14_RS20885anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (298/324);  minor base T (1/0);  total (299/324)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 624 total aligned reads displayed.

GGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACG  >  NC_008463/4564892‑4565187
                                                                                                                                                    |                                                                                                                                                   
  cgcAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACa                                                                                                                                                 <  2:2232274/151‑1 (MQ=255)
   gcAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAg                                                                                                                                                <  1:1134259/151‑1 (MQ=255)
    cAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAgg                                                                                                                                               >  2:3773992/1‑151 (MQ=255)
      aCTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCa                                                                                                                                             <  2:4179958/151‑1 (MQ=255)
       cTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAg                                                                                                                                            <  1:2604256/151‑1 (MQ=255)
                cAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTccccc                                                                                                                                   >  1:780954/1‑151 (MQ=255)
                cAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTccccc                                                                                                                                   <  1:2935579/151‑1 (MQ=255)
                 aGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCg                                                                                                                                  <  1:8864616/151‑1 (MQ=255)
                    cGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCAt                                                                                                                               >  2:9074601/1‑151 (MQ=255)
                     gATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATc                                                                                                                              <  2:7444893/151‑1 (MQ=255)
                     gATTGTCCAGGTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATc                                                                                                                              <  2:5641454/151‑1 (MQ=255)
                      aTTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCg                                                                                                                             <  2:2938195/151‑1 (MQ=255)
                      aTTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCg                                                                                                                             <  2:10994462/151‑1 (MQ=255)
                      aTTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCg                                                                                                                             >  2:4893024/1‑151 (MQ=255)
                      aTTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCg                                                                                                                             <  2:6287174/151‑1 (MQ=255)
                       ttGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGa                                                                                                                            <  2:609564/151‑1 (MQ=255)
                         gTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAgg                                                                                                                          <  1:5341345/151‑1 (MQ=255)
                           ccAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaa                                                                                                                        >  2:10711079/1‑151 (MQ=255)
                            cAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaag                                                                                                                       >  1:8341785/1‑151 (MQ=255)
                             aGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaaga                                                                                                                      <  1:9033083/151‑1 (MQ=255)
                              gTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaagaa                                                                                                                     >  2:10859421/1‑151 (MQ=255)
                              gTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaagaa                                                                                                                     <  2:5048849/151‑1 (MQ=255)
                                tCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGc                                                                                                                   <  1:10137241/151‑1 (MQ=255)
                                 ccGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCg                                                                                                                  <  1:9904412/151‑1 (MQ=255)
                                 ccGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCg                                                                                                                  <  2:10254045/151‑1 (MQ=255)
                                  cGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCgg                                                                                                                 <  2:6765990/151‑1 (MQ=255)
                                   gCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGt                                                                                                                <  1:5202496/151‑1 (MQ=255)
                                   gCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGt                                                                                                                <  2:7699433/151‑1 (MQ=255)
                                   gCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGt                                                                                                                >  2:1370685/1‑151 (MQ=255)
                                   gCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGt                                                                                                                >  2:3160958/1‑151 (MQ=255)
                                   gCATCGAAGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGt                                                                                                                >  2:1822634/1‑151 (MQ=255)
                                    cATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGtc                                                                                                               >  2:6248318/1‑150 (MQ=255)
                                    cATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTa                                                                                                               >  1:3030357/1‑151 (MQ=255)
                                        gATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACggg                                                                                                           <  1:427135/151‑1 (MQ=255)
                                         aTGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGc                                                                                                          <  2:6963013/151‑1 (MQ=255)
                                           ggCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTg                                                                                                        <  1:9379526/151‑1 (MQ=255)
                                           ggCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTg                                                                                                        <  1:8657556/151‑1 (MQ=255)
                                               ccccGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGGACTTCAATGCCGACTCCGGCGAAGCGCCGAACTGGCAGAAGTCCCCCGCAGCGAGGACGAAGCGGTACGGGCTGGGGt                                                                                                    >  2:2802879/1‑151 (MQ=255)
                                                cccGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGtt                                                                                                   <  2:8762761/151‑1 (MQ=255)
                                                   gggCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGACGAAGCGGTACGGGCTGGGGTTGcg                                                                                                >  1:10701007/1‑150 (MQ=255)
                                                     gCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCa                                                                                              <  2:9530599/151‑1 (MQ=255)
                                                       aTCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAgg                                                                                            >  1:3444058/1‑151 (MQ=255)
                                                       aTCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAgg                                                                                            >  2:7147783/1‑151 (MQ=255)
                                                           cgTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCCCCTCGCGACTCTCCGCGTCGTACTTCATTGCCGACTCCGGCGACGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGACGAAGCGGTACGGGCTGGGGTTGCGCAGGcacc                                                                                        >  1:6566772/1‑150 (MQ=255)
                                                            gTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACaa                                                                                       >  1:7326433/1‑151 (MQ=255)
                                                                gcgcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTgg                                                                                   >  1:5539582/1‑151 (MQ=255)
                                                                 cgcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGc                                                                                  <  2:9191694/151‑1 (MQ=255)
                                                                      gCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGTAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAg                                                                             <  1:1514939/151‑1 (MQ=255)
                                                                      gCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAg                                                                             <  2:4373506/151‑1 (MQ=255)
                                                                      gCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAg                                                                             <  1:4860574/151‑1 (MQ=255)
                                                                       cGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAgg                                                                            >  2:1053015/1‑151 (MQ=255)
                                                                           tGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCg                                                                        >  2:3953913/1‑151 (MQ=255)
                                                                            gCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGc                                                                       <  1:10813714/151‑1 (MQ=255)
                                                                            gCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGc                                                                       <  2:2594365/151‑1 (MQ=255)
                                                                             ccGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGcc                                                                      >  1:2445205/1‑151 (MQ=255)
                                                                             ccGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGcc                                                                      >  2:4640666/1‑151 (MQ=255)
                                                                             ccGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGcc                                                                      >  1:1914620/1‑151 (MQ=255)
                                                                              cGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCa                                                                     >  2:9373956/1‑151 (MQ=255)
                                                                               ggCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAg                                                                    <  1:11018989/151‑1 (MQ=255)
                                                                                gCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAgg                                                                   <  1:8231151/151‑1 (MQ=255)
                                                                                  aaTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAgggg                                                                 >  1:2807210/1‑151 (MQ=255)
                                                                                  aaTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAgggg                                                                 <  1:4673633/151‑1 (MQ=255)
                                                                                    tGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTc                                                               >  1:1285619/1‑151 (MQ=255)
                                                                                       ggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCcgc                                                            <  2:2432974/151‑1 (MQ=255)
                                                                                         tAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCgcgc                                                          >  2:5754047/1‑151 (MQ=255)
                                                                                          agagTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCa                                                         <  1:6693813/151‑1 (MQ=255)
                                                                                           gagTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCAc                                                        <  1:2245152/151‑1 (MQ=255)
                                                                                           gagTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCAc                                                        <  2:8066357/151‑1 (MQ=255)
                                                                                              ttCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGc                                                     >  1:3143957/1‑151 (MQ=255)
                                                                                                ccACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCAt                                                   <  1:1799645/151‑1 (MQ=255)
                                                                                                 cACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATg                                                  >  1:10279470/1‑151 (MQ=255)
                                                                                                 cACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATg                                                  <  2:3469252/151‑1 (MQ=255)
                                                                                                  aCCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAGCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGc                                                 >  1:1001245/1‑151 (MQ=255)
                                                                                                   ccTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCt                                                >  1:5006306/1‑151 (MQ=255)
                                                                                                   ccTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCt                                                <  1:5776959/151‑1 (MQ=255)
                                                                                                   ccTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCt                                                >  1:7626152/1‑151 (MQ=255)
                                                                                                         gACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCt                                          <  1:7734989/151‑1 (MQ=255)
                                                                                                          aCTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTg                                         >  1:5307628/1‑151 (MQ=255)
                                                                                                          aCTCTCCGCGTCGTACGTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTg                                         <  2:9828571/151‑1 (MQ=255)
                                                                                                            tctcCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGACGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgcg                                       >  2:4562571/1‑151 (MQ=255)
                                                                                                              tcCGCGTCGTACTTCAATGCCGACTCCGGCGACGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGcgcg                                     <  2:204147/151‑1 (MQ=255)
                                                                                                                  cgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACgg                                 >  1:6833205/1‑151 (MQ=255)
                                                                                                                      gtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCTCGGCATGCTGAAGCTGCGCGACGGTACg                             <  1:3607784/151‑1 (MQ=255)
                                                                                                                      gtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACg                             >  2:1174869/1‑151 (MQ=255)
                                                                                                                       tACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGa                            >  2:5606735/1‑151 (MQ=255)
                                                                                                                       tACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGa                            >  2:84572/1‑151 (MQ=255)
                                                                                                                          ttCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCt                         <  2:9644851/151‑1 (MQ=255)
                                                                                                                           tCAATGCCGACTCCGGCGAAGCGCCGACCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTg                        <  2:6488853/151‑1 (MQ=255)
                                                                                                                            cAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTgg                       <  1:6320934/151‑1 (MQ=255)
                                                                                                                             aaTGCCTACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGa                      >  1:10441995/1‑151 (MQ=255)
                                                                                                                             aaTGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGa                      >  2:5889712/1‑151 (MQ=255)
                                                                                                                                gCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAaca                   >  2:3634266/1‑151 (MQ=255)
                                                                                                                                 ccGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAacac                  <  1:9188258/151‑1 (MQ=255)
                                                                                                                                    aCTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTc               >  2:10142996/1‑151 (MQ=255)
                                                                                                                                     cTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGACGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCg              >  2:2701061/1‑151 (MQ=255)
                                                                                                                                     cTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTCCGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCg              >  2:8885873/1‑151 (MQ=255)
                                                                                                                                     cTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCg              <  2:8265282/151‑1 (MQ=255)
                                                                                                                                      tCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGc             >  1:9607145/1‑151 (MQ=255)
                                                                                                                                       ccGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGcc            >  2:157041/1‑151 (MQ=255)
                                                                                                                                             aaGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTCCGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCt      >  2:7932456/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACG  >  NC_008463/4564892‑4565187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑