Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,659,805 A→G intergenic (+45/+54) alpR → / ← PA14_RS21395 transcriptional regulator AlpR/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,659,8050AG100.0% 1572.3 / NA 400intergenic (+45/+54)alpR/PA14_RS21395transcriptional regulator AlpR/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (154/246);  total (154/246)

Only 100 of 419 total aligned reads displayed.

GCTTCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAAAAAAATCCCGGGCAAAAAAAAAGGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCCG  >  NC_008463/4659657‑4659953
                                                                                                                                                    |                                                                                                                                                    
gCTTCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACACCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGgaagaa                                                                                                                                                    >  1:9716830/1‑151 (MQ=255)
 cTTCAACAGCGACGAGCACCCGGACGATCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGaagaaa                                                                                                                                                   <  2:345799/151‑1 (MQ=255)
  ttCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGaagaaaa                                                                                                                                                  >  1:7611149/1‑151 (MQ=255)
    cAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAAt                                                                                                                                                <  1:11020506/151‑1 (MQ=255)
     aaCAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATc                                                                                                                                               <  2:8332586/151‑1 (MQ=255)
       cAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATccc                                                                                                                                             >  1:1312725/1‑151 (MQ=255)
        aGCGACGAGCACCCGGACGCGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGTCGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCg                                                                                                                                            >  2:8639787/1‑151 (MQ=255)
        aGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCg                                                                                                                                            <  1:4354894/151‑1 (MQ=255)
         gCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCgg                                                                                                                                           <  1:3967662/151‑1 (MQ=255)
          cgacgaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCggg                                                                                                                                          <  1:3101090/151‑1 (MQ=255)
           gacgaGCACCCGGACGAGCGCTACGAGCCCCAGGACGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGc                                                                                                                                         <  2:2019315/151‑1 (MQ=255)
           gacgaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGc                                                                                                                                         <  2:2266406/151‑1 (MQ=255)
             cgaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGTTCGGCCGGGTGTTCTGGTACTCGGTGCGGGTCTGAGCGCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGACGATAAATCCCGGGCaa                                                                                                                                       >  2:27453/1‑151 (MQ=255)
              gaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaat                                                                                                                                      >  1:1928303/1‑150 (MQ=255)
              gaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaa                                                                                                                                      >  1:4251835/1‑151 (MQ=255)
               aGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaag                                                                                                                                     <  2:4602314/151‑2 (MQ=255)
               aGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaag                                                                                                                                     <  2:148688/151‑2 (MQ=255)
                gCACCCGGACGAGCGCTACGAGCCCCAGGAATCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaaga                                                                                                                                    <  1:9530954/151‑3 (MQ=255)
                gCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAACAATCCCGGGCaaaga                                                                                                                                    >  2:9644035/1‑149 (MQ=255)
                gCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaaga                                                                                                                                    <  1:7860844/151‑3 (MQ=255)
                gCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaaga                                                                                                                                    <  2:10377911/151‑3 (MQ=255)
                 cACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaagaa                                                                                                                                   <  2:8077978/151‑1 (MQ=255)
                  aCCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaagaaa                                                                                                                                  <  1:7719821/151‑1 (MQ=255)
                    ccGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaagaaaaa                                                                                                                                <  1:4811001/151‑1 (MQ=255)
                     cGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAg                                                                                                                               <  1:2528388/151‑1 (MQ=255)
                      ggACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgg                                                                                                                              <  1:10011923/151‑1 (MQ=255)
                         cGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcc                                                                                                                           <  2:666663/151‑3 (MQ=255)
                         cGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcc                                                                                                                           <  2:5561883/151‑3 (MQ=255)
                          gAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcct                                                                                                                          <  1:3728528/151‑4 (MQ=255)
                          gAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcct                                                                                                                          <  1:5752555/151‑4 (MQ=255)
                          gAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcct                                                                                                                          >  2:5834772/1‑148 (MQ=255)
                               cTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAtt                                                                                                                    <  2:9492053/151‑1 (MQ=255)
                                tACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtg                                                                                                                   >  1:9966484/1‑151 (MQ=255)
                                       cccAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAgg                                                                                                            <  1:825500/151‑1 (MQ=255)
                                         cAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGcc                                                                                                          >  1:5296445/1‑151 (MQ=255)
                                           gtaaGGCGCCGAGCACTTCATCGTGTTCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACGCCTTCCCGCCCCGCCTGTCGTCGGGGCCGCGTTCCGGCGGAAGTAAAATCCCGGGCAATGAATAA‑GGGCCTGCATTGCTGCAGGCCCt                                                                                                        <  1:10135981/149‑1 (MQ=255)
                                            gAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCtt                                                                                                       <  2:6309313/151‑1 (MQ=255)
                                             aaGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAATTCCCGGGC‑AAAGAAAATGGGCCTGCATTGCTGCAGGCCCttt                                                                                                      >  2:3687548/1‑151 (MQ=255)
                                              aGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTc                                                                                                     <  1:10799886/151‑1 (MQ=255)
                                               gccgccGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTGCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCg                                                                                                    <  1:5896736/151‑1 (MQ=255)
                                                ccgccgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCgg                                                                                                   >  1:9388179/1‑151 (MQ=255)
                                                   ccgAGCACATCCACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTAt                                                                                                <  1:2380860/151‑1 (MQ=255)
                                                   ccgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTAt                                                                                                >  2:7500530/1‑151 (MQ=255)
                                                    cgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATg                                                                                               <  1:10331126/151‑1 (MQ=255)
                                                     gAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTCTgg                                                                                              >  1:7050706/1‑151 (MQ=255)
                                                      aGCACATCACCGTGCACGGCCGGGAGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACCGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAACGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGgc                                                                                             <  2:10645489/151‑1 (MQ=255)
                                                        cacaTCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGgcgc                                                                                           >  1:7144985/1‑151 (MQ=255)
                                                        cacaTCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGgcgc                                                                                           <  1:3472210/151‑1 (MQ=255)
                                                         ccaTCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTGCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCa                                                                                          <  2:6556842/150‑1 (MQ=255)
                                                         acaTCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCa                                                                                          >  2:408392/1‑151 (MQ=255)
                                                            tCAACGTGATCGGCCGGGTGGTCTGGTACTCGGTGCTGGTCGGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAACAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTc                                                                                       <  2:4760589/151‑1 (MQ=255)
                                                              aaCGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGACGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCag                                                                                     >  2:8210947/1‑151 (MQ=255)
                                                               aCGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCagg                                                                                    <  1:10678872/151‑1 (MQ=255)
                                                                cGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCagga                                                                                   >  1:7332702/1‑151 (MQ=255)
                                                                    aTCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCAGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGAt                                                                               <  2:7394882/151‑1 (MQ=255)
                                                                        gCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGa                                                                           <  2:9358226/151‑1 (MQ=255)
                                                                             gtgtTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTc                                                                      >  2:7128398/1‑151 (MQ=255)
                                                                                  cTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGAcc                                                                 <  2:2538785/151‑1 (MQ=255)
                                                                                       aCTCGGTGCTGGTCTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGGTCCGGCGGAAGAAACATCCCGGGCAAAGAAAAA‑GGGCCAGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCg                                                            <  2:9743540/151‑1 (MQ=38)
                                                                                         tCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGt                                                          <  2:6757971/151‑1 (MQ=255)
                                                                                           ggTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                        <  1:3594100/151‑1 (MQ=255)
                                                                                           ggTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGGTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                        <  1:6913328/151‑1 (MQ=255)
                                                                                            gTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAATTCCCGGGC‑AAAGAAAATGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGATCCTCCGACCGCTCGGTTCg                                                       >  2:1208530/1‑151 (MQ=38)
                                                                                               cTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                    <  1:20872/151‑1 (MQ=255)
                                                                                               cTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                    <  1:1801825/151‑1 (MQ=255)
                                                                                                tGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                   <  1:7992167/151‑1 (MQ=255)
                                                                                                  gTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCg                                                 <  1:2948341/151‑1 (MQ=255)
                                                                                                     tGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGt                                              <  1:6600146/151‑1 (MQ=255)
                                                                                                     tGACCCCTGCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGt                                              <  1:217684/151‑1 (MQ=255)
                                                                                                      gACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTGTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                             >  1:159631/1‑151 (MQ=255)
                                                                                                       aCCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAc                                            >  1:3259182/1‑151 (MQ=255)
                                                                                                       aCCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAc                                            <  1:4629033/151‑1 (MQ=255)
                                                                                                          ccTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAACATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActct                                         <  2:1399762/151‑1 (MQ=255)
                                                                                                            ttCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                       <  2:181249/151‑1 (MQ=255)
                                                                                                            gtCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                       <  1:1852914/150‑1 (MQ=255)
                                                                                                             tCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                      <  2:11046026/151‑1 (MQ=255)
                                                                                                                 gccccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc                                  >  2:2137074/1‑151 (MQ=255)
                                                                                                                  ccccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                 >  1:6245311/1‑151 (MQ=255)
                                                                                                                    ccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                               <  1:6730417/151‑1 (MQ=255)
                                                                                                                      gccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                             <  1:11153895/151‑1 (MQ=255)
                                                                                                                         tGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt                          >  2:853835/1‑151 (MQ=255)
                                                                                                                         tGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt                          <  1:2252925/151‑1 (MQ=255)
                                                                                                                         tGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt                          >  2:10449458/1‑151 (MQ=255)
                                                                                                                         tGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt                          <  2:169347/151‑1 (MQ=255)
                                                                                                                            cgacgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAg                       <  2:3970376/151‑1 (MQ=255)
                                                                                                                             gacgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGt                      >  2:7763140/1‑151 (MQ=255)
                                                                                                                               cgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGc                    >  1:2703714/1‑151 (MQ=255)
                                                                                                                               cgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGc                    >  1:4937213/1‑151 (MQ=255)
                                                                                                                               cgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGc                    <  1:560923/151‑1 (MQ=255)
                                                                                                                                  ggCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCgtg                 <  1:10493918/151‑1 (MQ=255)
                                                                                                                                  ggCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCgtg                 <  1:10282724/151‑1 (MQ=255)
                                                                                                                                  ggCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCgtg                 <  2:6007796/151‑1 (MQ=255)
                                                                                                                                      gcgTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCgg             >  2:7677693/1‑151 (MQ=255)
                                                                                                                                      gcgTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAAATGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCgg             >  1:8075307/1‑151 (MQ=255)
                                                                                                                                       cgTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTAAGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTCTCCAGCTGAGCTATGCGTGCGTGACGGt            <  2:3243114/151‑1 (MQ=21)
                                                                                                                                        gTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGGCGGTg           >  2:8853373/1‑151 (MQ=255)
                                                                                                                                        gTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGGCGGTg           >  1:8786014/1‑151 (MQ=255)
                                                                                                                                             ggcggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCtttt      <  2:7872880/151‑1 (MQ=255)
                                                                                                                                               cggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCttttac    <  1:910602/151‑3 (MQ=255)
                                                                                                                                                ggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTcc   <  2:4921099/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTTCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAAAAAAATCCCGGGCAAAAAAAAAGGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCCG  >  NC_008463/4659657‑4659953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑