Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 480,880 T→C A204A (GCA→GCG)  PA14_RS02220 ← 16S rRNA (uracil(1498)‑N(3))‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463480,8800TC99.8% 2025.2 / NA 504A204A (GCA→GCG) PA14_RS0222016S rRNA (uracil(1498)‑N(3))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (246/257);  minor base A (1/0);  total (247/257)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 504 total aligned reads displayed.

CACTGGCTGTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTTGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTTGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCC  >  NC_008463/480732‑481027
                                                                                                                                                    |                                                                                                                                                   
     gCTGTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCt                                                                                                                                              <  1:8617088/151‑1 (MQ=255)
      cTGTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTc                                                                                                                                             >  2:1247496/1‑151 (MQ=255)
         tCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCAc                                                                                                                                          <  2:4253802/151‑1 (MQ=255)
          cGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCAcc                                                                                                                                         >  1:713577/1‑151 (MQ=255)
           gCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCt                                                                                                                                        >  2:66858/1‑151 (MQ=255)
            ccGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTc                                                                                                                                       >  2:8571384/1‑151 (MQ=255)
            ccGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTc                                                                                                                                       <  1:2207262/151‑1 (MQ=255)
             cGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCg                                                                                                                                      >  1:11114464/1‑151 (MQ=255)
             cGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCg                                                                                                                                      >  2:721765/1‑151 (MQ=255)
                  tAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATc                                                                                                                                 >  1:1315702/1‑151 (MQ=255)
                   aaaGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCg                                                                                                                                >  1:9288326/1‑151 (MQ=255)
                   aaaGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCg                                                                                                                                >  1:9809697/1‑151 (MQ=255)
                       cggcggGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCa                                                                                                                            <  1:5830232/151‑1 (MQ=255)
                       cggcggGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCa                                                                                                                            >  1:3608650/1‑151 (MQ=255)
                        ggcggGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAg                                                                                                                           >  1:4632141/1‑151 (MQ=255)
                          cggGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGgc                                                                                                                         >  2:9742137/1‑151 (MQ=255)
                          cggGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCGCGGTGCGCGGCACGCGCGGCCCGCGGCGCGCGGCGAGGGCGCCGGCCGCCGACGCCAGCTCCACCTCGGCAGCGCTCAGgc                                                                                                                         >  2:6953541/1‑151 (MQ=255)
                           gggAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGgcc                                                                                                                        >  2:7048132/1‑151 (MQ=255)
                           gggAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGgcc                                                                                                                        <  1:4510127/151‑1 (MQ=255)
                             gagagaTATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGgccgc                                                                                                                      >  2:553745/1‑151 (MQ=255)
                               gagaTATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCt                                                                                                                    >  2:10100924/1‑151 (MQ=255)
                               gagaTATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCt                                                                                                                    >  1:9722374/1‑151 (MQ=255)
                                agaTATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCtt                                                                                                                   <  2:3558652/151‑1 (MQ=255)
                                 gaTATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCttc                                                                                                                  <  2:2788621/151‑2 (MQ=255)
                                  atatCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCttcg                                                                                                                 >  1:7003141/1‑149 (MQ=255)
                                    atCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCggg                                                                                                               <  1:5894290/151‑1 (MQ=255)
                                       aGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGAcc                                                                                                            <  1:3410858/151‑1 (MQ=255)
                                            aaGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACccacca                                                                                                       <  1:3801829/151‑1 (MQ=255)
                                            aaGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACccacca                                                                                                       <  1:9129602/151‑1 (MQ=255)
                                                    ccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGcc                                                                                               <  1:10846440/151‑1 (MQ=255)
                                                    ccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGcc                                                                                               >  1:6845779/1‑151 (MQ=255)
                                                      agagTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAg                                                                                             <  1:8315412/151‑1 (MQ=255)
                                                        agTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGACTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGgc                                                                                           <  1:6456492/151‑1 (MQ=255)
                                                         gTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGCCCCACCAGGAAGGCCAGgcg                                                                                          >  1:5764698/1‑151 (MQ=255)
                                                          ttGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGgcgc                                                                                         >  2:9031886/1‑151 (MQ=255)
                                                           tGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGcgcg                                                                                        <  2:985226/151‑1 (MQ=255)
                                                           tGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGcgcg                                                                                        <  1:10155861/151‑1 (MQ=255)
                                                            gCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGa                                                                                       <  1:2985221/151‑1 (MQ=255)
                                                             cTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAc                                                                                      <  1:8406213/151‑1 (MQ=255)
                                                               gggCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAcgg                                                                                    >  2:4908051/1‑151 (MQ=255)
                                                                 gCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAcggcg                                                                                  <  2:2966147/151‑1 (MQ=255)
                                                                   cACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACggcggc                                                                                >  1:10441939/1‑151 (MQ=255)
                                                                       gCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAt                                                                            <  2:6502975/151‑1 (MQ=255)
                                                                       gCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAt                                                                            <  1:5211095/151‑1 (MQ=255)
                                                                        ccAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtg                                                                           <  1:6048344/151‑1 (MQ=255)
                                                                         cAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACCtgg                                                                          >  2:3694109/1‑151 (MQ=255)
                                                                          aaCGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggc                                                                         >  1:5736171/1‑151 (MQ=255)
                                                                           aCGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggct                                                                        <  2:8811715/151‑1 (MQ=255)
                                                                               tACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGcc                                                                    >  2:1627627/1‑151 (MQ=255)
                                                                                accaccGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCa                                                                   >  2:3032209/1‑151 (MQ=255)
                                                                                accaccGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCa                                                                   <  2:6068695/151‑1 (MQ=255)
                                                                                accaccGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCa                                                                   <  2:7373165/151‑1 (MQ=255)
                                                                                  caccGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAgg                                                                 >  1:7421531/1‑151 (MQ=255)
                                                                                   accGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAggg                                                                >  2:3915851/1‑151 (MQ=255)
                                                                                    ccGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAgggg                                                               >  2:10221176/1‑151 (MQ=255)
                                                                                     cGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAggggg                                                              >  1:674833/1‑151 (MQ=255)
                                                                                      ggCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAgggggg                                                             >  2:8208024/1‑151 (MQ=255)
                                                                                         gcgGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGAGCGACGGCGGCACATGGCTGGCCAGGGGggcgg                                                          <  1:319125/151‑1 (MQ=255)
                                                                                            gTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCca                                                       >  1:4019077/1‑151 (MQ=255)
                                                                                             tctcGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCcac                                                      <  1:2329664/151‑1 (MQ=255)
                                                                                               tcGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAg                                                    <  2:8484327/151‑1 (MQ=255)
                                                                                                  gTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGt                                                 <  2:10464627/151‑1 (MQ=255)
                                                                                                  gTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGt                                                 <  2:9450743/151‑1 (MQ=255)
                                                                                                  gTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGt                                                 >  2:2448634/1‑151 (MQ=255)
                                                                                                     cgcAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCa                                              <  2:9161893/151‑1 (MQ=255)
                                                                                                     cgcAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCa                                              <  1:502426/151‑1 (MQ=255)
                                                                                                      gcagcaCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAg                                             >  2:4695625/1‑151 (MQ=255)
                                                                                                        agcaCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGa                                           <  2:1895239/151‑1 (MQ=255)
                                                                                                         gcaCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGAc                                          <  1:9470185/151‑1 (MQ=255)
                                                                                                          caCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGAcc                                         >  1:7484822/1‑151 (MQ=255)
                                                                                                          caCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGAcc                                         <  2:1706151/151‑1 (MQ=255)
                                                                                                           aCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCa                                        >  2:7813722/1‑151 (MQ=255)
                                                                                                           aCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCa                                        <  2:5601019/151‑1 (MQ=255)
                                                                                                               gcgGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCt                                    >  1:4257081/1‑151 (MQ=255)
                                                                                                               gcgGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCt                                    <  2:2170807/151‑1 (MQ=255)
                                                                                                                cgGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTg                                   <  2:800156/151‑1 (MQ=255)
                                                                                                                 gggCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGa                                  <  1:2751443/151‑1 (MQ=255)
                                                                                                                      gAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCa                             >  2:5842341/1‑151 (MQ=255)
                                                                                                                      gAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCa                             >  1:254051/1‑151 (MQ=255)
                                                                                                                       aGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCAt                            >  2:2431026/1‑151 (MQ=255)
                                                                                                                        ggCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATc                           <  1:5873053/151‑1 (MQ=255)
                                                                                                                          cgcgcgGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGt                         <  1:1733111/151‑1 (MQ=255)
                                                                                                                          cgcgcgGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGt                         <  2:2041124/151‑1 (MQ=255)
                                                                                                                             gcggcgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTccc                      <  2:7557627/151‑1 (MQ=255)
                                                                                                                                   tGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCccgcccgc                >  1:8169895/1‑151 (MQ=255)
                                                                                                                                    ggtaGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGCGCTCATCGTCCCGCCcgcg               >  1:7929997/4‑151 (MQ=255)
                                                                                                                                    ggAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCcgcg               >  2:4118379/1‑151 (MQ=255)
                                                                                                                                    ggAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCcgcg               <  2:1517670/151‑1 (MQ=255)
                                                                                                                                      aaGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCa             <  1:9966656/151‑1 (MQ=255)
                                                                                                                                        gccggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGc           >  2:3307782/1‑151 (MQ=255)
                                                                                                                                         ccggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGcc          <  1:5474950/151‑1 (MQ=255)
                                                                                                                                          cggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCa         <  2:5298597/151‑1 (MQ=255)
                                                                                                                                          cggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCa         >  1:145812/1‑151 (MQ=255)
                                                                                                                                           ggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAg        >  2:5040286/1‑151 (MQ=255)
                                                                                                                                           ggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAg        >  1:1024016/1‑151 (MQ=255)
                                                                                                                                             ccgccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtc      >  1:9025428/1‑151 (MQ=255)
                                                                                                                                               gccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcct    <  2:9526012/151‑1 (MQ=255)
                                                                                                                                                ccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcctc   >  1:10614580/1‑151 (MQ=255)
                                                                                                                                                 cTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcctcc  >  1:6148136/1‑151 (MQ=255)
                                                                                                                                                 cTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcctcc  >  1:7540682/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CACTGGCTGTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTTGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTTGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCC  >  NC_008463/480732‑481027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑