Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,997,786 T→C K173E (AAG→GAG)  PA14_RS22840 ← DUF853 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,997,7860TC100.0% 1962.8 / NA 486K173E (AAG→GAG) PA14_RS22840DUF853 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (215/271);  total (215/271)

Only 100 of 486 total aligned reads displayed.

CGCCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTTGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGGC  >  NC_008463/4997638‑4997933
                                                                                                                                                    |                                                                                                                                                   
cgcCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGc                                                                                                                                                   <  1:9582174/151‑1 (MQ=255)
 gcCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGcc                                                                                                                                                  <  1:2761152/151‑1 (MQ=255)
 gcCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGcc                                                                                                                                                  <  1:662395/151‑1 (MQ=255)
 gcCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGcc                                                                                                                                                  <  1:7794894/151‑1 (MQ=255)
  cccccGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCg                                                                                                                                                 >  1:5036436/1‑151 (MQ=255)
   ccccGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGa                                                                                                                                                <  1:5933412/151‑1 (MQ=255)
    cccgcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAg                                                                                                                                               <  2:3907050/151‑1 (MQ=255)
     ccgcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAgg                                                                                                                                              >  2:5561176/1‑151 (MQ=255)
       gcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAt                                                                                                                                            <  2:5675346/151‑1 (MQ=255)
       gcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAt                                                                                                                                            >  1:340085/1‑151 (MQ=255)
        cccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtc                                                                                                                                           <  1:10793963/151‑1 (MQ=255)
        cccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtc                                                                                                                                           >  2:2796261/1‑151 (MQ=255)
           gTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTcc                                                                                                                                        >  1:376757/1‑151 (MQ=255)
           gTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTcc                                                                                                                                        <  1:1622582/151‑1 (MQ=255)
             ccGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGACGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCgg                                                                                                                                      <  2:9926176/151‑1 (MQ=255)
              cGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGa                                                                                                                                     >  1:8431962/1‑151 (MQ=255)
               ggCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGAt                                                                                                                                    <  2:8713262/151‑1 (MQ=255)
                 cTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGCAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTg                                                                                                                                  <  1:3508124/151‑1 (MQ=255)
                 cTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTg                                                                                                                                  >  1:9929631/1‑151 (MQ=255)
                 cTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTg                                                                                                                                  <  1:5158767/151‑1 (MQ=255)
                 cTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTg                                                                                                                                  >  1:10496361/1‑151 (MQ=255)
                  tCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGt                                                                                                                                 >  1:8525855/1‑151 (MQ=255)
                   cGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGACCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTc                                                                                                                                >  1:826983/1‑151 (MQ=255)
                    ggggCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTcc                                                                                                                               <  2:8974147/151‑1 (MQ=255)
                      ggCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCtt                                                                                                                             <  2:1640616/151‑1 (MQ=255)
                       gCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTc                                                                                                                            <  2:923030/151‑1 (MQ=255)
                        cGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCa                                                                                                                           <  2:452741/151‑1 (MQ=255)
                        cGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCa                                                                                                                           <  1:118448/151‑1 (MQ=255)
                         ggaggaTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAg                                                                                                                          >  2:147794/1‑151 (MQ=255)
                         ggaggaTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAg                                                                                                                          >  1:2516157/1‑151 (MQ=255)
                           aggaTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAggt                                                                                                                        <  1:7720752/151‑1 (MQ=255)
                              aTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGCTTGTCCTTCAggtggt                                                                                                                     >  2:6656968/1‑151 (MQ=255)
                                 tCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGa                                                                                                                  <  1:3307347/151‑1 (MQ=255)
                                    tCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagca                                                                                                               <  2:1191112/151‑1 (MQ=255)
                                       aGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAgcg                                                                                                            >  1:7091980/1‑151 (MQ=255)
                                          tGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGcct                                                                                                         >  1:2686020/1‑151 (MQ=255)
                                              gggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcag                                                                                                     >  2:6555900/1‑151 (MQ=255)
                                               ggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcagg                                                                                                    >  2:2376842/1‑151 (MQ=255)
                                                gcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggt                                                                                                   >  1:2592953/1‑151 (MQ=255)
                                                 caggCTCGCCTAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtc                                                                                                  <  1:13230/151‑1 (MQ=255)
                                                 caggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccgtcaggtc                                                                                                  >  1:6914917/1‑151 (MQ=255)
                                                  aggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGNCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcc                                                                                                 >  1:5853268/1‑151 (MQ=255)
                                                    gCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcctt                                                                                               >  2:2178917/1‑151 (MQ=255)
                                                    gCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcctt                                                                                               >  2:3233826/1‑151 (MQ=255)
                                                      tCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttca                                                                                             <  1:1718540/151‑1 (MQ=255)
                                                        gCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttcagg                                                                                           <  2:346375/151‑1 (MQ=255)
                                                        gCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttcagg                                                                                           >  1:9498731/1‑151 (MQ=255)
                                                        gCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttcagg                                                                                           >  2:2604043/1‑151 (MQ=255)
                                                        gCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttcagg                                                                                           <  2:3423321/151‑1 (MQ=255)
                                                          cGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCcttcaggtccttcaggtc                                                                                         >  1:9389846/1‑151 (MQ=255)
                                                          cGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCcttcaggtccttcaggtc                                                                                         >  1:10659790/1‑151 (MQ=255)
                                                           gaagaaGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCg                                                                                        <  1:7408920/151‑1 (MQ=255)
                                                            aagaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGa                                                                                       >  2:6390649/1‑151 (MQ=255)
                                                               aagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagca                                                                                    <  2:589005/151‑1 (MQ=255)
                                                                  gCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagca                                                                                 <  1:11174768/151‑1 (MQ=255)
                                                                   ccTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagcag                                                                                <  1:8768136/151‑1 (MQ=255)
                                                                   ccTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagcag                                                                                <  2:7196128/151‑1 (MQ=255)
                                                                        gcgcCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGc                                                                           <  1:2273847/151‑1 (MQ=255)
                                                                               gctgctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCg                                                                    >  1:9053172/1‑151 (MQ=255)
                                                                                ctgctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCgg                                                                   <  2:7757372/151‑1 (MQ=255)
                                                                                    tCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCgg                                                               >  1:10005500/1‑151 (MQ=255)
                                                                                    tCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCgg                                                               >  2:4958907/1‑151 (MQ=255)
                                                                                       agagTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCa                                                            >  1:2983884/1‑151 (MQ=255)
                                                                                        gagTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCAc                                                           >  1:6258064/1‑151 (MQ=255)
                                                                                          gTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCt                                                         <  2:8290375/151‑1 (MQ=255)
                                                                                          gTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCt                                                         >  2:7983317/1‑151 (MQ=255)
                                                                                          gTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCt                                                         <  1:6492968/151‑1 (MQ=255)
                                                                                           tGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTg                                                        >  2:7547392/1‑151 (MQ=255)
                                                                                            ggCCAGGCGGCGCAGCAGCGCCTTCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTgg                                                       <  1:358090/151‑1 (MQ=255)
                                                                                            ggCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTgg                                                       <  1:8926230/151‑1 (MQ=255)
                                                                                             gCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGa                                                      >  1:390780/1‑151 (MQ=255)
                                                                                              ccAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGaa                                                     <  2:10952197/151‑1 (MQ=255)
                                                                                                aGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAgg                                                   >  1:10158332/1‑151 (MQ=255)
                                                                                                     gcgcAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGgcggcg                                              <  2:9048055/151‑1 (MQ=255)
                                                                                                        cagcagCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAAc                                           >  1:473979/1‑151 (MQ=255)
                                                                                                         agcagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACa                                          <  1:4186011/151‑1 (MQ=255)
                                                                                                          gcagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAg                                         <  2:4163231/151‑1 (MQ=255)
                                                                                                          gcagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAg                                         >  1:9121659/1‑151 (MQ=255)
                                                                                                           cagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgc                                        <  1:5990873/151‑1 (MQ=255)
                                                                                                            agcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgcg                                       <  2:11025542/151‑1 (MQ=255)
                                                                                                             gcgcCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgcgc                                      <  2:6104556/151‑1 (MQ=255)
                                                                                                                 cTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGcc                                  >  2:6298233/1‑151 (MQ=255)
                                                                                                                  tGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCct                                 <  1:5414300/151‑1 (MQ=255)
                                                                                                                   gCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCctg                                >  2:4707619/1‑151 (MQ=255)
                                                                                                                     ccGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCctgct                              >  2:6890889/1‑151 (MQ=255)
                                                                                                                      cGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGAGTGTCCTTCCGGTGGTTGAGCAGCGCCTTCAGGTCCGTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCctgctg                             <  2:8528284/151‑1 (MQ=255)
                                                                                                                       ggACGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTgg                            >  1:4507294/1‑151 (MQ=255)
                                                                                                                        gAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGc                           >  1:4429662/1‑151 (MQ=255)
                                                                                                                             gcCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCg                      >  2:1915470/1‑151 (MQ=255)
                                                                                                                                ggTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTc                   <  2:3062478/151‑1 (MQ=255)
                                                                                                                                ggTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTc                   <  2:981567/151‑1 (MQ=255)
                                                                                                                                  tGAACAGCGCCCGGTCCTCGCCGAGAATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCaa                 >  1:1836571/1‑151 (MQ=255)
                                                                                                                                   gAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAAc                <  1:4957779/151‑1 (MQ=255)
                                                                                                                                      cAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTcc             <  1:7031759/151‑1 (MQ=255)
                                                                                                                                         cgcCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCagg          <  2:10563978/151‑1 (MQ=255)
                                                                                                                                         cgcCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCagg          <  2:5372576/151‑1 (MQ=255)
                                                                                                                                            ccGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCaggagg       <  2:1773391/151‑1 (MQ=255)
                                                                                                                                            ccGGTCCTCGCCGAGGATCTCCGGATTGTCCGTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTAACGGACAACTCCaggagg       <  2:10248857/151‑1 (MQ=255)
                                                                                                                                               gTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTCTCGGTCAACTCCAGGAGGTTg    >  1:1953966/1‑151 (MQ=255)
                                                                                                                                                tCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTgg   >  1:10306946/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTTGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGGC  >  NC_008463/4997638‑4997933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑