Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,116,457 G→A A482V (GCC→GTC)  ftsI ← peptidoglycan D,D‑transpeptidase FtsI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,116,4570GA85.3% 2023.2 / 237.8 586A482V (GCC→GTC) ftsIpeptidoglycan D,D‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base G (38/48);  new base A (269/231);  total (307/279)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 597 total aligned reads displayed.

AGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGACTCGGGTCATGCTCAGCGGCA  >  NC_008463/5116308‑5116607
                                                                                                                                                     |                                                                                                                                                      
  cccGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCcgacgg                                                                                                                                                     >  1:1181883/1‑151 (MQ=255)
     gCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGt                                                                                                                                                  >  1:1636322/1‑151 (MQ=255)
      ccTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTg                                                                                                                                                 >  1:4189863/1‑151 (MQ=255)
       cTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTgg                                                                                                                                                >  1:3095802/1‑151 (MQ=255)
        ttGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGt                                                                                                                                               <  1:9252348/151‑1 (MQ=255)
         tGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTa                                                                                                                                              >  2:4077602/1‑151 (MQ=255)
          gctcggctcgTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATCGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGCCCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTaa                                                                                                                                             >  2:6833771/1‑151 (MQ=255)
          gctcggctcgTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTaa                                                                                                                                             >  1:4052699/1‑151 (MQ=255)
              ggctcgTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCg                                                                                                                                         >  1:8748197/1‑151 (MQ=255)
              ggctcgTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCg                                                                                                                                         <  2:3099180/151‑1 (MQ=255)
               gctcgTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCgg                                                                                                                                        <  2:8846681/151‑1 (MQ=255)
               gctcgTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCgg                                                                                                                                        >  2:5720066/1‑151 (MQ=255)
                 tcgtcgATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCa                                                                                                                                      >  1:8582459/1‑151 (MQ=255)
                   gtcgATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCAcc                                                                                                                                    >  1:3701801/1‑151 (MQ=255)
                    tcgATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCt                                                                                                                                   >  1:1859952/1‑151 (MQ=255)
                      gATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGGAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTgg                                                                                                                                 <  2:8299152/151‑1 (MQ=255)
                                aCCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAaca                                                                                                                       <  1:9810008/151‑1 (MQ=255)
                                aCCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAaca                                                                                                                       >  1:6050377/1‑151 (MQ=255)
                                 ccATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAacac                                                                                                                      >  2:1526164/1‑151 (MQ=255)
                                 ccATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAacac                                                                                                                      >  1:7304957/1‑151 (MQ=255)
                                  cATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACAcc                                                                                                                     >  1:666902/1‑151 (MQ=255)
                                    tCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACAcccc                                                                                                                   <  1:4737788/151‑1 (MQ=255)
                                    tCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACAcccc                                                                                                                   <  1:639504/151‑1 (MQ=255)
                                     cgcgATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCg                                                                                                                  >  1:2480537/1‑151 (MQ=255)
                                       cgATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGcc                                                                                                                >  1:5252602/1‑151 (MQ=255)
                                        gATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGccc                                                                                                               >  2:672010/1‑151 (MQ=255)
                                         aTGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCt                                                                                                              <  1:11119178/151‑1 (MQ=255)
                                         aTGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCt                                                                                                              >  1:803556/1‑151 (MQ=255)
                                           gcgcGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTgg                                                                                                            >  1:6581944/1‑151 (MQ=255)
                                             gcgGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGc                                                                                                          <  1:7952006/151‑1 (MQ=255)
                                             gcgGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGc                                                                                                          >  2:2848459/1‑151 (MQ=255)
                                                gATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTc                                                                                                       <  1:4366308/151‑1 (MQ=255)
                                                 aTCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCg                                                                                                      <  2:2178157/151‑1 (MQ=255)
                                                        gCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACtt                                                                                               >  1:4453959/1‑151 (MQ=255)
                                                          cGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCTGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACttgt                                                                                             >  2:6044510/1‑151 (MQ=255)
                                                          cGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACttgt                                                                                             >  2:5329415/1‑151 (MQ=255)
                                                            gggCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACttgttg                                                                                           <  2:7430015/151‑1 (MQ=255)
                                                              gCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTgca                                                                                         <  2:4838609/151‑1 (MQ=255)
                                                                gAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTgcagc                                                                                       <  1:2933134/151‑1 (MQ=255)
                                                                gAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTgcagc                                                                                       <  1:498555/151‑1 (MQ=255)
                                                                 aaaCCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTgcagca                                                                                      <  1:7006064/151‑1 (MQ=255)
                                                                  aaCCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCAt                                                                                     <  2:5065719/151‑1 (MQ=255)
                                                                  aaCCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCAt                                                                                     <  1:5572943/151‑1 (MQ=255)
                                                                  aaCCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCAt                                                                                     >  2:7220090/1‑151 (MQ=255)
                                                                    ccGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGc                                                                                   <  1:10175121/151‑1 (MQ=255)
                                                                      ggCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGccc                                                                                 >  1:9510971/1‑151 (MQ=255)
                                                                       gCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCt                                                                                <  2:467706/151‑1 (MQ=255)
                                                                         gAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGc                                                                              >  1:8169531/1‑151 (MQ=255)
                                                                            cAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACg                                                                           >  2:1754044/1‑151 (MQ=255)
                                                                            cAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACg                                                                           <  2:1930434/151‑1 (MQ=255)
                                                                              gcgagcgaTAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGt                                                                         >  1:7174851/1‑151 (MQ=255)
                                                                               cgagcgaTAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTg                                                                        >  2:426083/1‑151 (MQ=255)
                                                                                gagcgaTAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTgg                                                                       >  1:3473275/1‑151 (MQ=255)
                                                                                 agcgaTAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGa                                                                      >  2:10172351/1‑151 (MQ=255)
                                                                                   cgaTAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAg                                                                    >  1:8862818/1‑151 (MQ=255)
                                                                                     aTAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGcc                                                                  >  2:7657258/1‑151 (MQ=255)
                                                                                       aGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCAc                                                                >  2:5205370/1‑151 (MQ=255)
                                                                                       aGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCAc                                                                >  2:805037/1‑151 (MQ=255)
                                                                                       aGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCAc                                                                >  2:6376561/1‑151 (MQ=255)
                                                                                        ggCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACt                                                               >  1:9975274/1‑151 (MQ=255)
                                                                                         gCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACtt                                                              <  1:969965/151‑1 (MQ=255)
                                                                                            ttttCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCg                                                           <  1:3575102/151‑1 (MQ=255)
                                                                                             tttCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCgg                                                          <  2:10622760/151‑1 (MQ=255)
                                                                                              ttCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGc                                                         >  1:10970198/1‑151 (MQ=255)
                                                                                                 ccGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCgag                                                      >  2:4317258/1‑151 (MQ=255)
                                                                                                  cGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCgaga                                                     <  1:4892844/151‑1 (MQ=255)
                                                                                                    gTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATc                                                   <  2:9393963/151‑1 (MQ=255)
                                                                                                    gTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATc                                                   >  2:11101774/1‑151 (MQ=255)
                                                                                                    gTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATc                                                   >  2:8620778/1‑151 (MQ=255)
                                                                                                    gTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATc                                                   >  1:10063498/1‑151 (MQ=255)
                                                                                                     tAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCa                                                  <  1:744265/151‑1 (MQ=255)
                                                                                                     tAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCa                                                  <  1:9141636/151‑1 (MQ=255)
                                                                                                       gCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCAcc                                                <  1:10400216/151‑1 (MQ=255)
                                                                                                        cccTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCt                                               <  2:341921/151‑1 (MQ=255)
                                                                                                         ccTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTg                                              >  2:1244342/1‑151 (MQ=255)
                                                                                                           ttGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCa                                            <  1:8414217/151‑1 (MQ=255)
                                                                                                            tGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCAc                                           <  1:3200520/151‑1 (MQ=255)
                                                                                                             ggTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACg                                          >  2:2448102/1‑151 (MQ=255)
                                                                                                             ggTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACg                                          >  1:150871/1‑151 (MQ=255)
                                                                                                              gTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACATGCACGc                                         >  2:9171989/1‑151 (MQ=255)
                                                                                                                    accgaGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCg                                   <  2:7863480/151‑1 (MQ=255)
                                                                                                                     ccgaGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCgg                                  <  1:2804195/151‑1 (MQ=255)
                                                                                                                      cgaGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGc                                 <  1:6934726/151‑1 (MQ=255)
                                                                                                                      cgaGACCTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGc                                 >  1:10479597/1‑151 (MQ=255)
                                                                                                                        agaCCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCAc                               >  2:623527/1‑151 (MQ=255)
                                                                                                                            cTTGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCgg                           >  1:2118954/1‑151 (MQ=255)
                                                                                                                             ttGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGt                          <  2:3611212/151‑1 (MQ=255)
                                                                                                                              tGCGCGCGGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTc                         <  2:702611/151‑1 (MQ=255)
                                                                                                                                 gcgcgGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGAc                      >  1:7639014/1‑151 (MQ=255)
                                                                                                                                 gcgcgGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGAc                      >  1:5463426/1‑151 (MQ=255)
                                                                                                                                    cgGTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGACTCg                   >  1:5691605/1‑151 (MQ=255)
                                                                                                                                     ggTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGACTCgg                  >  1:9060298/1‑151 (MQ=255)
                                                                                                                                      gTCCCGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGACTCggg                 <  1:10709765/151‑1 (MQ=255)
                                                                                                                                        cccGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGAGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGACTCGGGTc               <  1:2565192/151‑1 (MQ=255)
                                                                                                                                         ccGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGACTCGGGTCa              <  2:9802910/151‑1 (MQ=255)
                                                                                                                                          cGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGACTCGGGTCAt             >  1:2307332/1‑151 (MQ=255)
                                                                                                                                          cGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGACTCGGGTCAt             >  1:5986067/1‑151 (MQ=255)
                                                                                                                                          cGCTCTTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGACTCGGGTCAt             <  1:7750055/151‑1 (MQ=255)
                                                                                                                                            ctctTGCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGACTCGGGTCATGc           <  2:10946131/151‑1 (MQ=255)
                                                                                                                                                 gCCGACGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGACTCGGGTCATGCTCAGc      >  1:4557760/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAAGCCACTTCCGGCGAGATCACCTGCACGCCATCCGGCACGCGGTCGACTCGGGTCATGCTCAGCGGCA  >  NC_008463/5116308‑5116607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑