Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 732,660 G→A intergenic (+114/‑426) tyrS → / → PA14_RS03450 tyrosine‑‑tRNA ligase/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463732,6600GA98.6% 241.1 / ‑4.2 70intergenic (+114/‑426)tyrS/PA14_RS03450tyrosine‑‑tRNA ligase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (42/27);  total (42/28)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

AGGCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGGGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAGCGGTTCGGTCCC  >  NC_008463/732512‑732706
                                                                                                                                                    |                                              
agGCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagt                                              <  1:1808550/151‑1 (MQ=255)
 ggCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtg                                             <  2:10911413/151‑1 (MQ=255)
 ggCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtg                                             >  1:1158787/1‑151 (MQ=255)
 ggCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtg                                             <  1:8000611/151‑1 (MQ=255)
 ggCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtg                                             >  1:6167775/1‑151 (MQ=255)
 ggCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtg                                             >  2:4473/1‑151 (MQ=255)
  gCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                            >  1:8687449/1‑151 (MQ=255)
  gCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                            >  2:1353268/1‑151 (MQ=255)
  gCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                            <  1:6584757/151‑1 (MQ=255)
  gCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                            >  2:2957358/1‑151 (MQ=255)
   cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAACTAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                                           <  2:10944149/151‑1 (MQ=255)
   cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                                           <  1:9425012/151‑1 (MQ=255)
   cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                                           <  2:5325537/151‑1 (MQ=255)
   cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                                           >  2:6182025/1‑151 (MQ=255)
   cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                                           <  1:9424825/151‑1 (MQ=255)
    tttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                                          >  1:5419526/1‑151 (MQ=255)
    tttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                                          >  2:7901782/1‑151 (MQ=255)
    tttCGCGCGCATCACGCTGAAAGCTGAATAAGTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                                          <  2:11017853/151‑1 (MQ=255)
     ttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGa                                         >  2:8568136/1‑151 (MQ=255)
      tCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGaa                                        <  1:5637399/151‑1 (MQ=255)
      tCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGaa                                        <  2:3396129/151‑1 (MQ=255)
       cgcgcgCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                                       >  1:457666/1‑151 (MQ=255)
       cgcgcgCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTCCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                                       <  2:4777397/151‑1 (MQ=255)
        gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgc                                      >  2:4864219/1‑151 (MQ=255)
        gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgc                                      >  1:9740651/1‑151 (MQ=255)
        gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgc                                      <  1:8461407/151‑1 (MQ=255)
        gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgc                                      <  2:680523/151‑1 (MQ=255)
         cgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                                     <  1:4261497/151‑1 (MQ=255)
         cgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                                     >  1:3224803/1‑151 (MQ=255)
         cgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                                     >  2:2199897/1‑151 (MQ=255)
         cgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                                     >  2:3171603/1‑151 (MQ=255)
         cgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCACCCAGGAGTGGTGAAgcg                                     >  2:2199898/1‑151 (MQ=255)
         cgcgcATCACGCGGAAAGCTGAATCATTCTTTCAAATAAGTGGTGACGCCAGTTACTCTCTCCCTATCATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                                     <  2:8015960/151‑1 (MQ=14)
          gcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcgc                                    >  2:11066111/1‑151 (MQ=255)
           cgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCt                                   <  2:10339462/151‑1 (MQ=255)
           cgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCt                                   <  2:3915297/151‑1 (MQ=255)
           cgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCt                                   <  2:535724/151‑1 (MQ=255)
            gcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGATGCGCTc                                  >  2:9672818/1‑151 (MQ=255)
            gcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                                  >  1:334286/1‑151 (MQ=255)
             cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                                 <  2:10192248/151‑1 (MQ=255)
             cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                                 <  1:6266453/151‑1 (MQ=255)
             cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAAGGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                                 >  2:5552351/1‑151 (MQ=255)
               tCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCgg                               <  1:10435203/151‑1 (MQ=255)
               tCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCgg                               >  2:5548779/1‑151 (MQ=255)
               tCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCgg                               >  1:8632424/1‑151 (MQ=255)
                cACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGc                              >  1:6105102/1‑151 (MQ=255)
                  cGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAg                            <  2:7084739/151‑1 (MQ=255)
                  cGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAg                            <  1:8237197/151‑1 (MQ=255)
                   gCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAgg                           >  1:434882/1‑151 (MQ=255)
                    cTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgt                          <  1:6549388/151‑1 (MQ=255)
                     tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt                         >  1:8089761/1‑151 (MQ=255)
                     tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt                         >  2:7051920/1‑151 (MQ=255)
                      gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAATGATTTACGGATTCTCTCCCAGGAGTGTTGAAGCGCTCCGGCAGgttg                        >  2:1720509/1‑151 (MQ=255)
                       aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGTATTCTCTCACAGGAGTGGTGAAGCGCTCCGGCAGgttgt                       >  2:9601123/1‑151 (MQ=255)
                       aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgt                       >  1:5281999/1‑151 (MQ=38)
                        aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt                      >  1:3515997/1‑151 (MQ=38)
                        aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt                      >  2:10239857/1‑151 (MQ=38)
                        aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt                      <  2:1423785/151‑1 (MQ=38)
                        aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt                      >  1:3526999/1‑151 (MQ=38)
                         aGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTc                     >  1:10625189/1‑151 (MQ=37)
                          gCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcg                    >  1:625338/1‑151 (MQ=25)
                           cTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgc                   <  2:3773900/151‑1 (MQ=25)
                            tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATNCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc                  >  1:5854282/1‑151 (MQ=21)
                            tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc                  >  2:3158497/1‑151 (MQ=21)
                            tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc                  <  2:3073757/151‑1 (MQ=21)
                            tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc                  >  1:3869141/1‑151 (MQ=21)
                            tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc                  >  1:6077400/1‑151 (MQ=21)
                             gAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg                 >  1:9112314/1‑151 (MQ=18)
                             gAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCCATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg                 <  1:10083154/151‑1 (MQ=18)
                              aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccgc                <  2:3326264/151‑1 (MQ=18)
                              aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccgc                >  2:3280560/1‑151 (MQ=18)
                              aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccgc                <  1:1835883/151‑1 (MQ=18)
                              aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccgc                <  2:1214348/151‑1 (MQ=18)
                               ataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCa               <  1:9460124/151‑1 (MQ=17)
                               ataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCa               >  1:7036172/1‑151 (MQ=17)
                               ataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCa               >  1:7033019/1‑151 (MQ=17)
                                taatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg              >  2:8963737/1‑151 (MQ=14)
                                taatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg              <  2:1985286/151‑1 (MQ=14)
                                 aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAGc             >  2:10806237/1‑151 (MQ=14)
                                 aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAGc             >  1:2724961/1‑151 (MQ=14)
                                 aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAGc             >  2:8005799/1‑151 (MQ=14)
                                 aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAGc             >  2:8013028/1‑151 (MQ=14)
                                 aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAGc             >  1:8723039/1‑151 (MQ=14)
                                 aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAGc             <  1:4805837/151‑1 (MQ=14)
                                            atcaGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGGGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAGCGGTTCGGTccc  <  1:3268913/148‑1 (MQ=1)
                                                                                                                                                    |                                              
AGGCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGGGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAGCGGTTCGGTCCC  >  NC_008463/732512‑732706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑