Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 927,917 T→C I56T (ATC→ACC)  PA14_RS04340 → PAS domain S‑box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463927,9170TC99.7% 2381.7 / NA 593I56T (ATC→ACC) PA14_RS04340PAS domain S‑box protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (242/349);  minor base A (1/1);  total (243/350)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 610 total aligned reads displayed.

AAGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGATCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGCGGCTT  >  NC_008463/927769‑928064
                                                                                                                                                    |                                                                                                                                                   
  gTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACccgc                                                                                                                                                 >  1:10312947/1‑151 (MQ=255)
   ttCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACccgcc                                                                                                                                                >  1:9332506/1‑151 (MQ=255)
   ttCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACccgcc                                                                                                                                                <  1:4321695/151‑1 (MQ=255)
    tCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACccgccg                                                                                                                                               <  1:5023673/151‑1 (MQ=255)
      aGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCcgcg                                                                                                                                             >  2:8033428/1‑151 (MQ=255)
        gCTGAGCCGCGGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGAGGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTg                                                                                                                                           <  1:8498956/151‑1 (MQ=255)
         cTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTgg                                                                                                                                          >  1:6439305/1‑151 (MQ=255)
          tGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGCGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGa                                                                                                                                         <  2:4176297/151‑1 (MQ=255)
                 cTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTAc                                                                                                                                  >  2:2801094/1‑151 (MQ=255)
                   gggcgggcGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCa                                                                                                                                <  2:248731/151‑1 (MQ=255)
                   gggcgggcGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCa                                                                                                                                <  2:9298078/151‑1 (MQ=255)
                       gggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTAt                                                                                                                            <  2:10281311/151‑1 (MQ=255)
                           gcgACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCt                                                                                                                        <  1:1833765/151‑1 (MQ=255)
                            cgACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCtt                                                                                                                       <  2:6977170/151‑1 (MQ=255)
                            cgACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTAGGGCtt                                                                                                                       <  2:9719515/151‑1 (MQ=255)
                             gACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTg                                                                                                                      <  1:7111843/151‑1 (MQ=255)
                             gACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTg                                                                                                                      <  2:9791478/151‑1 (MQ=255)
                               cacTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCg                                                                                                                    >  1:10736414/1‑151 (MQ=255)
                                  tGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTgg                                                                                                                 >  1:1258030/1‑151 (MQ=255)
                                  tGGCGCTCGGACTGTTGCGGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTgg                                                                                                                 <  2:6259707/151‑1 (MQ=255)
                                   ggCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGAGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTggg                                                                                                                >  1:3646099/1‑151 (MQ=255)
                                   ggCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCAGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTggg                                                                                                                <  2:2006174/151‑1 (MQ=255)
                                       cTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTgc                                                                                                            <  1:10923237/151‑1 (MQ=255)
                                        tCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTgcg                                                                                                           >  1:4460335/1‑151 (MQ=255)
                                          ggACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGcgcg                                                                                                         >  2:3293848/1‑151 (MQ=255)
                                           gACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCgg                                                                                                        >  2:10770601/1‑151 (MQ=255)
                                            aCTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGt                                                                                                       >  2:2987870/1‑151 (MQ=255)
                                            aCTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGt                                                                                                       <  1:2283823/151‑1 (MQ=255)
                                               gTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGcc                                                                                                    >  1:1715112/1‑151 (MQ=255)
                                                    tGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCt                                                                                               <  2:8901239/151‑1 (MQ=255)
                                                    tGTCCGGGCGGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGACCTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCt                                                                                               >  1:5814646/1‑151 (MQ=255)
                                                    agacagggCTGGGCGCCTGGGGGCAGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCCCTGGAACGCTTGGCGTAACTGCTGTCCGAGTCGGTGAACCTCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCt                                                                                               >  2:671385/6‑151 (MQ=255)
                                                     gTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTg                                                                                              <  2:5459844/151‑1 (MQ=255)
                                                     gTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCGTGCTg                                                                                              <  2:7570137/151‑1 (MQ=255)
                                                      tCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCCACGAGCTACAGGCGCGGATGGCGCTGGACCGCGAGGCGGTCCTGCTGGCAGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGa                                                                                             >  2:5841295/1‑151 (MQ=255)
                                                      tCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGa                                                                                             <  1:5347166/151‑1 (MQ=255)
                                                      tCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGa                                                                                             <  1:1370225/151‑1 (MQ=255)
                                                       ccGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGAc                                                                                            <  1:6338579/151‑1 (MQ=255)
                                                        cGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACa                                                                                           <  2:530599/151‑1 (MQ=255)
                                                         gggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAg                                                                                          <  1:6147204/151‑1 (MQ=255)
                                                          ggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGc                                                                                         >  1:2051892/1‑151 (MQ=255)
                                                           gCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCCGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCg                                                                                        >  2:11115751/1‑151 (MQ=255)
                                                           gCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCg                                                                                        <  2:4885365/151‑1 (MQ=255)
                                                             tGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCggg                                                                                      <  2:8687220/151‑1 (MQ=255)
                                                             tGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCggg                                                                                      <  1:562493/151‑1 (MQ=255)
                                                               ggCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCg                                                                                    <  1:10052094/151‑1 (MQ=255)
                                                                       ggggCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGcac                                                                            <  1:6897675/151‑1 (MQ=255)
                                                                        gggCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGcaca                                                                           >  2:5097251/1‑151 (MQ=255)
                                                                          gCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATc                                                                         <  2:8208680/151‑1 (MQ=255)
                                                                          gCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATc                                                                         >  2:8329205/1‑151 (MQ=255)
                                                                          gCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATc                                                                         <  1:3303817/151‑1 (MQ=255)
                                                                             ggCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGAc                                                                      <  1:9469123/151‑1 (MQ=255)
                                                                             ggCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGAc                                                                      >  1:3169229/1‑151 (MQ=255)
                                                                              gcgcTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGAcc                                                                     <  1:7511478/151‑1 (MQ=255)
                                                                               cgcTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACcg                                                                    >  2:9653673/1‑151 (MQ=255)
                                                                                 cgggTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACcgcg                                                                  <  2:306167/149‑1 (MQ=255)
                                                                                 cTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACcgcg                                                                  <  2:9915101/151‑1 (MQ=255)
                                                                                    gTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGAGAGGCGCACATCGACCGCGAAc                                                               >  2:10181198/1‑151 (MQ=255)
                                                                                    gTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACCGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAAc                                                               <  2:6352023/151‑1 (MQ=255)
                                                                                     tCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACt                                                              >  2:4076118/1‑151 (MQ=255)
                                                                                          gAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACCGCGGGCGAGGCGCACATCGACCGCGAACTGTTcc                                                         <  1:7370/151‑1 (MQ=255)
                                                                                          gAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTcc                                                         <  2:2160690/151‑1 (MQ=255)
                                                                                          gAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTcc                                                         >  1:6626751/1‑151 (MQ=255)
                                                                                          gAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTcc                                                         >  2:5533641/1‑151 (MQ=255)
                                                                                            gCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTccgc                                                       <  2:3652968/151‑1 (MQ=255)
                                                                                              aaCAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTccgccg                                                     >  1:7768347/1‑151 (MQ=255)
                                                                                                 aGGCGCGGATGGCGCTGGAACGCGAGGCGGACCTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTa                                                  <  2:6606418/151‑1 (MQ=255)
                                                                                                   gcgcGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACa                                                <  2:11002106/151‑1 (MQ=255)
                                                                                                      cgGCTGGCGCTGGATCGCGAGGCGGAACGGCTGGCCGAGGCGGTGCCCCGCCGCGTGGATCTCTACCTGTTTGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGcc                                             <  2:10518689/151‑1 (MQ=255)
                                                                                                      cgGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGcc                                             >  2:1373541/1‑151 (MQ=255)
                                                                                                      cgGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGcc                                             >  1:2526960/1‑151 (MQ=255)
                                                                                                      cgGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGcc                                             <  2:4588549/151‑1 (MQ=255)
                                                                                                       ggATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCt                                            >  1:1059905/1‑151 (MQ=255)
                                                                                                         aTGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACACTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGa                                          <  1:8280757/151‑1 (MQ=255)
                                                                                                           ggCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGAcc                                        <  1:11045165/151‑1 (MQ=255)
                                                                                                            gcgcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGAccc                                       <  2:8474649/151‑1 (MQ=255)
                                                                                                            gcgcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGAccc                                       <  1:4104635/151‑1 (MQ=255)
                                                                                                              gcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGACCTCTACCAGTAGGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCcgc                                     <  1:9103558/151‑1 (MQ=255)
                                                                                                              gcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCcgc                                     >  1:2614217/1‑151 (MQ=255)
                                                                                                                   accgcgAGGCGGAACTGCGGGCCGAGGCGGTGCCCCGCCGCGTGGAACTCTACCAGGATGTCTTGCGTGGGGTGCGCGGTGCCTTGCTGCCAGCGGGCGAGGCGCACCTCGACCGCGAACTGTTCCGCCGCTACCGCCTGACCCGCGACAt                                <  2:1835426/149‑1 (MQ=255)
                                                                                                                   aaCGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACAt                                >  1:522131/1‑151 (MQ=255)
                                                                                                                    aCGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATc                               <  1:2875683/151‑1 (MQ=255)
                                                                                                                      gcgAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGa                             >  1:7889871/1‑151 (MQ=255)
                                                                                                                        gAGGCGGATCTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCTGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGAcc                           <  2:3947919/151‑1 (MQ=255)
                                                                                                                        gAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGAcc                           >  2:4789233/1‑151 (MQ=255)
                                                                                                                        gAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGAcc                           >  2:7841556/1‑151 (MQ=255)
                                                                                                                        gAGGCGGAACTGCGGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGAcc                           <  1:3775218/151‑1 (MQ=255)
                                                                                                                         aGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACcg                          <  1:9969795/151‑1 (MQ=255)
                                                                                                                             ggAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAg                      >  1:3524171/1‑151 (MQ=255)
                                                                                                                                aCTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGGGCGCGGTGCCGTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACGGTTCCGCCGCGACAGCCTGACCCGCGACATCGACCGCGAGttg                   >  1:6738225/1‑150 (MQ=255)
                                                                                                                                 ctgctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTc                  >  2:7402744/1‑151 (MQ=255)
                                                                                                                                   gctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTccc                <  1:466621/151‑1 (MQ=255)
                                                                                                                                   gctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTccc                >  1:865808/1‑151 (MQ=255)
                                                                                                                                    ctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCg               >  2:4327616/1‑151 (MQ=255)
                                                                                                                                     tgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCgg              <  2:9198214/151‑1 (MQ=255)
                                                                                                                                     tgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCgg              >  1:806814/1‑151 (MQ=255)
                                                                                                                                      ggCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGgc             <  1:4005128/151‑1 (MQ=255)
                                                                                                                                             gCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcgcg      <  1:2957551/151‑1 (MQ=255)
                                                                                                                                               ggTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGACGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcggc    >  2:1606047/1‑151 (MQ=255)
                                                                                                                                                 tGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcggctt  <  1:8928431/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGATCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGCGGCTT  >  NC_008463/927769‑928064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑