Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,157,979 G→A Q372* (CAG→TAG)  dacB ← D‑alanyl‑D‑alanine carboxypeptidase/D‑alanyl‑D‑alanine‑endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,157,9790GA99.2% 2135.9 / NA 517Q372* (CAG→TAG) dacBD‑alanyl‑D‑alanine carboxypeptidase/D‑alanyl‑D‑alanine‑endopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (270/243);  minor base C (2/2);  total (272/245)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 517 total aligned reads displayed.

TCGCCGACCAGTGCGGTGCGCCGCAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTGGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGCGGAACTGCGCGCCGATGGAGAGGAACA  >  NC_008463/2157831‑2158125
                                                                                                                                                    |                                                                                                                                                  
  gCCGACCAGTGCGGTGCGCCGCAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAgcgc                                                                                                                                                <  1:1148938/151‑1 (MQ=255)
     gACCAGTGCGGTGCGCCGCAGGCGTTTTCGCAAGGTGCCGTCGAGGCCGGCCCGCGGCAGCGAGGAGATGTACTCCCCTGCATAGGGGCTGTGCCAGGCGGCTTTCAGCAAAGCCGCCAACTCACGGGCGCAGACCCGTTCCTAGCGCGAc                                                                                                                                             >  2:5253527/1‑151 (MQ=255)
     gACCAGTGCGGTGCGCCGCAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGAc                                                                                                                                             >  2:10191545/1‑151 (MQ=255)
     gACCAGTGCGGTGCGCCGCAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGAc                                                                                                                                             >  1:10641840/1‑151 (MQ=255)
       ccAGTGCGGTGCGCCGCAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAg                                                                                                                                           <  1:16931/151‑1 (MQ=255)
       ccAGTGCGGTGCGCCGCAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTACTAGCGCGACAg                                                                                                                                           >  2:5866829/1‑151 (MQ=255)
         aGTGCGGTGCGCCGCAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTc                                                                                                                                         >  1:4143397/1‑151 (MQ=255)
            gcggtgcgCCGCAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCg                                                                                                                                      <  2:2320624/151‑1 (MQ=255)
             cggtgcgCCGCAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCTTCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGa                                                                                                                                     <  2:11154097/151‑1 (MQ=255)
                tgcgCCGCAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGcc                                                                                                                                  <  2:435694/151‑1 (MQ=255)
                tgcgCCGCAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGcc                                                                                                                                  <  1:762313/151‑1 (MQ=255)
                  cgccgcAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCGTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGt                                                                                                                                <  2:6566712/151‑1 (MQ=255)
                   gccgcAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGtt                                                                                                                               >  2:2600034/1‑151 (MQ=255)
                   gccgcAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGtt                                                                                                                               >  1:6289401/1‑151 (MQ=255)
                    ccgcAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTtc                                                                                                                              >  2:8512931/1‑151 (MQ=255)
                    ccgcAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTtc                                                                                                                              >  1:3813025/1‑151 (MQ=255)
                    ccgcAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTtc                                                                                                                              >  1:90491/1‑151 (MQ=255)
                     cgcAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTtct                                                                                                                             >  1:7116435/1‑151 (MQ=255)
                     cgcAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTtct                                                                                                                             <  1:1089821/151‑1 (MQ=255)
                       cAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTcc                                                                                                                           <  2:9489909/151‑1 (MQ=255)
                         ggCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCAt                                                                                                                         <  2:548842/151‑1 (MQ=255)
                           cGTTTGCGCATGGTGCCGTCGAGGCCGGCCCGCGGCAGCGAGGAGATGTACTCCGCAGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCa                                                                                                                       <  2:2929802/151‑1 (MQ=255)
                           cGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCa                                                                                                                       >  1:3486607/1‑151 (MQ=255)
                            gTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCAc                                                                                                                      >  2:1795820/1‑151 (MQ=255)
                            gTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCAc                                                                                                                      >  2:3414120/1‑151 (MQ=255)
                            gTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCAc                                                                                                                      >  1:10773085/1‑151 (MQ=255)
                            gTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGAGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCAc                                                                                                                      <  1:6377755/151‑1 (MQ=255)
                             tttGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCAcc                                                                                                                     >  2:8792043/1‑151 (MQ=255)
                              ttGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCa                                                                                                                    >  2:9110657/1‑151 (MQ=255)
                              gtGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCa                                                                                                                    <  2:1958109/150‑1 (MQ=255)
                               tGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAg                                                                                                                   >  1:2320934/1‑151 (MQ=255)
                                 cgcATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAggc                                                                                                                 >  2:10614206/1‑151 (MQ=255)
                                 cgcATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAggc                                                                                                                 <  2:11037560/151‑1 (MQ=255)
                                 cgcATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAggc                                                                                                                 >  2:566527/1‑151 (MQ=255)
                                   cATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAggcgc                                                                                                               >  2:1278920/1‑151 (MQ=255)
                                    aTGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGTCCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACAAggcgcg                                                                                                              <  1:7964618/151‑1 (MQ=255)
                                       gTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAggcgcggcg                                                                                                           >  1:8575409/1‑151 (MQ=255)
                                        tGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAggcgcggcgc                                                                                                          <  2:2210717/151‑1 (MQ=255)
                                         gCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGgcgcggcgcg                                                                                                         >  1:6543088/1‑151 (MQ=255)
                                           cGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGt                                                                                                       >  2:395360/1‑151 (MQ=255)
                                            gTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTg                                                                                                      >  1:2611086/1‑151 (MQ=255)
                                             tCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGa                                                                                                     >  2:3201922/1‑151 (MQ=255)
                                               gAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATg                                                                                                   >  1:5536888/1‑151 (MQ=255)
                                                 ggccggccAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGcc                                                                                                 >  1:5823124/1‑151 (MQ=255)
                                                      gccAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTgc                                                                                            <  1:10006757/151‑1 (MQ=255)
                                                      gccAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTgc                                                                                            >  2:3084528/1‑151 (MQ=255)
                                                       ccAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTgcg                                                                                           <  2:6725821/151‑1 (MQ=255)
                                                           cGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCgcc                                                                                       <  2:5320307/151‑1 (MQ=255)
                                                            ggCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCgcca                                                                                      <  2:1664900/151‑1 (MQ=255)
                                                               aGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCgccagcc                                                                                   >  1:5377943/1‑151 (MQ=255)
                                                               aGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCgccagcc                                                                                   >  1:10389989/1‑151 (MQ=255)
                                                                  gaggagATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACt                                                                                >  1:8702082/1‑151 (MQ=255)
                                                                   aggagATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTg                                                                               >  2:4667881/1‑151 (MQ=255)
                                                                   aggagATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTg                                                                               >  1:5198470/1‑151 (MQ=255)
                                                                    ggagATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGc                                                                              <  2:1554962/151‑1 (MQ=255)
                                                                      agaTGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCg                                                                            <  1:9509199/151‑1 (MQ=255)
                                                                           tACTCCGCTGCATAGGGTCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGcacca                                                                       <  2:8871295/151‑1 (MQ=255)
                                                                           tACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCACCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGcacca                                                                       >  1:3125132/1‑151 (MQ=255)
                                                                             cTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCaccacc                                                                     <  1:600159/151‑1 (MQ=255)
                                                                                 gCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCt                                                                 <  1:1849418/151‑1 (MQ=255)
                                                                                  cTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTg                                                                >  1:11049337/1‑151 (MQ=255)
                                                                                  cTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTg                                                                >  1:7369339/1‑151 (MQ=255)
                                                                                   tGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTgc                                                               >  1:4602827/1‑151 (MQ=255)
                                                                                   tGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTgc                                                               >  1:4841678/1‑151 (MQ=255)
                                                                                    gCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTgcg                                                              >  2:6280258/1‑151 (MQ=255)
                                                                                    gCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTgcg                                                              <  1:1916155/151‑1 (MQ=255)
                                                                                       tAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCgg                                                           <  1:2906547/151‑1 (MQ=255)
                                                                                       tAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCgg                                                           >  1:5289110/1‑151 (MQ=255)
                                                                                          gggCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCt                                                        >  2:3735518/1‑151 (MQ=255)
                                                                                              tgtgCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGc                                                    <  2:7092519/151‑1 (MQ=255)
                                                                                               gtgCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGcg                                                   >  1:9850417/1‑151 (MQ=255)
                                                                                                tgCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGcgt                                                  <  2:7289020/151‑1 (MQ=255)
                                                                                                 gCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGcgtc                                                 >  1:9341176/1‑151 (MQ=255)
                                                                                                 gCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCGGCGCGCCCTGGGcgtc                                                 >  2:9067022/1‑151 (MQ=255)
                                                                                                  ccAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGcgtcg                                                >  1:3991855/1‑151 (MQ=255)
                                                                                                    aGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCgtcgtc                                              <  1:10245284/151‑1 (MQ=255)
                                                                                                    aGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCGAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCgtcgtc                                              <  2:9253101/151‑1 (MQ=255)
                                                                                                     ggcggcTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGtcgtcg                                             <  1:3355078/151‑1 (MQ=255)
                                                                                                     ggcggcTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGtcgtcg                                             <  1:6779893/151‑1 (MQ=255)
                                                                                                       cggcTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGcc                                           <  1:4713807/151‑1 (MQ=255)
                                                                                                       cggcTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGcc                                           <  1:6920954/151‑1 (MQ=255)
                                                                                                       cggcTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTACTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGcc                                           <  2:6926276/151‑1 (MQ=255)
                                                                                                         gcTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGt                                         >  2:614428/1‑151 (MQ=255)
                                                                                                         gcTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGt                                         <  2:10491836/151‑1 (MQ=255)
                                                                                                            tGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCgg                                      >  1:2177358/1‑151 (MQ=255)
                                                                                                             gcagcaTCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGgc                                     <  1:775025/151‑1 (MQ=255)
                                                                                                                 caTCGCCGCCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTg                                 <  1:5591077/151‑1 (MQ=255)
                                                                                                                    cgccgcCATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTg                              >  2:10797295/1‑151 (MQ=255)
                                                                                                                     gccgccATCTCACGGGCGCTGACCCGTTCGTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGc                             >  2:4499833/1‑151 (MQ=255)
                                                                                                                     gccgccATCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGc                             >  2:7754534/1‑151 (MQ=255)
                                                                                                                           aTCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGCGGAACt                       <  1:2948805/151‑1 (MQ=255)
                                                                                                                           aTCTCACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGCGGAACt                       <  1:8311800/151‑1 (MQ=255)
                                                                                                                            tcgcACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGGTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGCGGAACTg                      <  2:9874955/148‑1 (MQ=255)
                                                                                                                             ctcACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGCGGAACTgc                     >  2:8393550/1‑151 (MQ=255)
                                                                                                                              tcACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGCGGAACTgcg                    <  1:1368472/151‑1 (MQ=255)
                                                                                                                               cACGGGCGCTGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGCGGAACTgcgc                   >  1:1464427/1‑151 (MQ=255)
                                                                                                                                        tGACCCGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGCGGAACTGCGCGCCGATGga          <  2:1005991/151‑1 (MQ=255)
                                                                                                                                            ccGTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGCGGAACTGCGCGCCGATGGAGAgg      <  2:6394030/151‑1 (MQ=255)
                                                                                                                                              gTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGCGGAACTGCGCGCCGATGGAGAGGaa    <  2:4960242/151‑1 (MQ=255)
                                                                                                                                              gTTCCTAGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGCGGAACTGCGCGCCGATGGAGAGGaa    <  1:9719307/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCGCCGACCAGTGCGGTGCGCCGCAGGCGTTTGCGCATGGTGCCGTCGAGGCCGGCCAGCGGCAGCGAGGAGATGTACTCCGCTGCATAGGGGCTGTGCCAGGCGGCTTGCAGCATCGCCGCCATCTCACGGGCGCTGACCCGTTCCTGGCGCGACAGTCCCGAGCCGTTCTCCATCACCAGGCGCGGCGCGGTGATGCCCTTGCGCGCCAGCCACTGCCGCACCACCCGCTGCGCGGCCTGGGCGTCGTCGCCGTCGGCGCTGTTGCGGAACTGCGCGCCGATGGAGAGGAACA  >  NC_008463/2157831‑2158125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑