Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084636,206,0690TG55.6% 306.5 / inf 717intergenic (‑174/+23)PA14_RS28345/PA14_RS28350type VI secretion system tip protein VgrG/Hcp family type VI secretion system effector
Reads supporting (aligned to +/- strand):  ref base T (163/155);  new base G (197/201);  total (361/356)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 720 total aligned reads displayed.

CGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGTTGCCCGGGTCC  >  NC_008463/6205920‑6206218
                                                                                                                                                     |                                                                                                                                                     
  aCCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTgg                                                                                                                                                     >  2:10494149/1‑150 (MQ=34)
          gattGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTgc                                                                                                                                             >  1:6340619/1‑150 (MQ=32)
           cttGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACACCAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTgcg                                                                                                                                            <  1:5696338/149‑1 (MQ=17)
               tCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGcggcgg                                                                                                                                        >  2:8195338/1‑150 (MQ=32)
                cctgcctgGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGt                                                                                                                                       <  1:1329050/150‑1 (MQ=32)
                       ggCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCt                                                                                                                                >  1:1890207/1‑150 (MQ=35)
                             ggCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCg                                                                                                                          >  1:766349/1‑150 (MQ=17)
                                gagaCAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCg                                                                                                                       >  1:3361151/1‑150 (MQ=17)
                                 agaCAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCCCGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGa                                                                                                                      >  2:4626466/1‑150 (MQ=37)
                                  gaCAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGAc                                                                                                                     >  1:2068705/1‑150 (MQ=17)
                                     aaGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCgg                                                                                                                  >  1:6428706/1‑150 (MQ=17)
                                      aGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCggg                                                                                                                 >  2:871141/1‑150 (MQ=17)
                                      aGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCggg                                                                                                                 <  1:4251028/150‑1 (MQ=17)
                                        ggCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGa                                                                                                               <  2:5973589/150‑1 (MQ=17)
                                          cgcgcAGGCTCGATCGGAAGCTGCCTACACCAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAgc                                                                                                             <  1:10992917/150‑1 (MQ=37)
                                           gcgcAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGGCATCGGGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAgcg                                                                                                            >  2:6764913/1‑150 (MQ=12)
                                            cgcAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGGCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGGGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAgcgc                                                                                                           >  2:3177196/1‑150 (MQ=12)
                                                ggCTCGATCGGTAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCGAgt                                                                                                       >  2:4187248/1‑150 (MQ=12)
                                                   gcGATCGGAAGCTGCCTACCACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAgtcgt                                                                                                    <  1:8070651/149‑1 (MQ=37)
                                                      aTCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCgg                                                                                                 >  1:8838823/1‑150 (MQ=37)
                                                         ggAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGc                                                                                              <  1:6017791/150‑1 (MQ=17)
                                                         ggAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCACCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGc                                                                                              <  1:3507004/150‑1 (MQ=37)
                                                              cTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAg                                                                                         >  1:462678/1‑150 (MQ=17)
                                                               tGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAgg                                                                                        <  1:2853177/150‑1 (MQ=37)
                                                               tGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAgg                                                                                        <  1:7791830/150‑1 (MQ=17)
                                                               tGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAgg                                                                                        >  1:8250627/1‑150 (MQ=17)
                                                                gCCTACCACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGt                                                                                       <  2:5163265/150‑1 (MQ=37)
                                                                 ccTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTg                                                                                      <  2:3909125/150‑1 (MQ=37)
                                                                  cTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGc                                                                                     <  1:10929171/150‑1 (MQ=17)
                                                                  cTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCGGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGc                                                                                     <  2:4848896/150‑1 (MQ=37)
                                                                   tACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGcc                                                                                    >  1:302910/1‑150 (MQ=37)
                                                                    acaacaAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCg                                                                                   <  2:3480778/150‑1 (MQ=17)
                                                                      aacaaAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGga                                                                                 >  1:1439694/1‑150 (MQ=17)
                                                                       acaaAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGgag                                                                                <  1:10585056/150‑1 (MQ=17)
                                                                          aaGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACt                                                                             <  2:5411778/150‑1 (MQ=37)
                                                                           aGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACtt                                                                            <  2:9400735/150‑1 (MQ=37)
                                                                           aGCGGCAGCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCGGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACtt                                                                            >  2:2922184/1‑150 (MQ=12)
                                                                               gCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTg                                                                        >  1:9805162/1‑150 (MQ=37)
                                                                               gCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTg                                                                        >  2:6961908/1‑150 (MQ=17)
                                                                               gCATCGACCCGGCGCGGGCTTTCCTGGCCAAGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGTCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTg                                                                        >  1:447437/1‑150 (MQ=12)
                                                                                cATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTgg                                                                       >  2:6444745/1‑150 (MQ=17)
                                                                                  tCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGTCGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGCGCCGGAGGTGCCGGCGACGGCGTgggg                                                                     >  2:10812376/1‑149 (MQ=11)
                                                                                   cGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTc                                                                    >  2:1545318/1‑150 (MQ=37)
                                                                                     aCCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCa                                                                  >  1:8531976/1‑150 (MQ=17)
                                                                                     aCCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCa                                                                  >  1:7429748/1‑150 (MQ=17)
                                                                                     aCCCGGCGCGGGCATTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCa                                                                  <  1:2329256/150‑1 (MQ=17)
                                                                                        cGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGt                                                               <  1:7975130/150‑1 (MQ=17)
                                                                                        cGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGt                                                               >  2:9162845/1‑150 (MQ=17)
                                                                                         ggCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTg                                                              >  1:2575455/1‑150 (MQ=17)
                                                                                           cgcgGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGAt                                                            <  1:4925587/150‑1 (MQ=17)
                                                                                             cgGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCt                                                          <  1:5468484/150‑1 (MQ=37)
                                                                                              gggCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCtt                                                         >  1:10345333/1‑150 (MQ=37)
                                                                                              gggCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCtt                                                         >  1:386214/1‑150 (MQ=17)
                                                                                               ggCTTTCCTGGCCACGGCGCTGTGGGTCATCGGGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTg                                                        <  1:10006367/150‑1 (MQ=17)
                                                                                                gCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGc                                                       >  2:6346221/1‑150 (MQ=37)
                                                                                                gCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGc                                                       <  1:72225/150‑1 (MQ=17)
                                                                                                 cTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCg                                                      <  2:8467961/150‑1 (MQ=37)
                                                                                                 cTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCg                                                      <  1:5301802/150‑1 (MQ=37)
                                                                                                 cTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCg                                                      <  1:6896859/150‑1 (MQ=17)
                                                                                                    tCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTa                                                   >  2:9598516/1‑150 (MQ=37)
                                                                                                    tCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTa                                                   >  2:10099860/1‑150 (MQ=17)
                                                                                                    tCCTGGACACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTa                                                   <  2:789018/150‑1 (MQ=17)
                                                                                                      cTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGTTGCCGGAGACTACGTGGGTCCAGGTGATCTTGCGGTAgg                                                 <  2:2820436/150‑1 (MQ=255)
                                                                                                       tGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGt                                                >  1:8443470/1‑150 (MQ=37)
                                                                                                       tGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGt                                                >  1:1790764/1‑150 (MQ=37)
                                                                                                        ggCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTg                                               >  1:6556174/1‑150 (MQ=17)
                                                                                                        ggCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTg                                               <  2:7114271/150‑1 (MQ=17)
                                                                                                               gcgcTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCa                                        >  1:9253496/1‑150 (MQ=37)
                                                                                                                 gcTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACg                                      <  2:7697432/150‑1 (MQ=17)
                                                                                                                  cTGTGGGTCATCGCGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGt                                     <  2:3029121/150‑1 (MQ=17)
                                                                                                                  cTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGt                                     <  1:583948/150‑1 (MQ=37)
                                                                                                                  cTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGt                                     >  1:3746092/1‑150 (MQ=17)
                                                                                                                    gtgGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCt                                   >  1:953053/1‑150 (MQ=37)
                                                                                                                    gtgGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCt                                   <  2:165963/150‑1 (MQ=17)
                                                                                                                     tgGGTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCtt                                  <  2:5876083/150‑1 (MQ=17)
                                                                                                                       ggTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTcc                                >  2:333913/1‑150 (MQ=17)
                                                                                                                       ggTCATCGAGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTcc                                >  2:5547823/1‑150 (MQ=17)
                                                                                                                           aTCGAGGGCACGACGGCCAGCGCGGATGGCCGCGTGCGGCGGTCGCGGCTCAGACGTCGACCGGGGAGCGCATGTCGTCGGAGCCGTAGGTGCCGGAGACTTCTTGGGTCCTGGTTATCTTGCTGTCGGTTATTTGCACGTATGCCTggt                            <  2:9714984/150‑1 (MQ=11)
                                                                                                                            tCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAggtg                           >  1:8649525/1‑150 (MQ=37)
                                                                                                                               aGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGTCTTCGGGGGTCCTGGTGATCTTTCGGTAGGTGAAGTGCACGTCTTCCAggggggt                        >  1:2666685/1‑150 (MQ=11)
                                                                                                                               aGGGCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAggtgggt                        <  2:2427425/150‑1 (MQ=17)
                                                                                                                                gggCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAggtgggtg                       >  2:6077042/1‑150 (MQ=17)
                                                                                                                                gggCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAggtgggtg                       >  2:2245569/1‑150 (MQ=17)
                                                                                                                                 ggCACGACGGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGa                      <  1:6679507/150‑1 (MQ=17)
                                                                                                                                     cgacgGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTg                  <  2:10303035/150‑1 (MQ=37)
                                                                                                                                     cgacgGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCTTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTg                  <  1:7062816/150‑1 (MQ=17)
                                                                                                                                      gacgGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGACGTGCACGTCTTCCAGGTGGGTGAAGTgg                 >  1:9117472/1‑150 (MQ=17)
                                                                                                                                      gacgGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTgg                 <  1:4012610/150‑1 (MQ=17)
                                                                                                                                       acgGCCAGCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGGGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTggg                >  2:7499947/1‑150 (MQ=17)
                                                                                                                                          gCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGt             <  2:6249931/150‑1 (MQ=37)
                                                                                                                                           ccAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGtt            >  2:3646094/1‑150 (MQ=37)
                                                                                                                                             aGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGTTGc          <  2:11037903/150‑1 (MQ=37)
                                                                                                                                              gCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGTTGcc         >  2:4465969/1‑150 (MQ=37)
                                                                                                                                              gCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGTTGcc         <  2:2067453/150‑1 (MQ=17)
                                                                                                                                              gCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGTTGcc         <  2:6604047/150‑1 (MQ=17)
                                                                                                                                              gCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGTTGcc         <  2:2580929/150‑1 (MQ=17)
                                                                                                                                              gCGAGGCGGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGTTGcc         >  1:10243744/1‑150 (MQ=17)
                                                                                                                                               cGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGTTGccc        <  1:10693863/150‑1 (MQ=37)
                                                                                                                                                gAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGTTGCCCg       <  1:5174461/150‑1 (MQ=37)
                                                                                                                                                   gCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCTCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGTTGCCCgggg    >  2:8939375/1‑149 (MQ=37)
                                                                                                                                                     |                                                                                                                                                     
CGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTTTCCTGGCCACGGCGCTGTGGGTCATCGAGGGCACGACGGCCAGCGAGGCTGGCCGCGTGCGGCGGTCGCGGCTCAGCCGGCGACCGGGGAGCGCCAGTCGTCGGAGCCGGAGGTGCCGGAGACTTCGTGGGTCCAGGTGATCTTGCGGTAGGTGAAGTGCACGTCTTCCAGGTGGGTGAAGTGGGCGTTGCCCGGGTCC  >  NC_008463/6205920‑6206218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑