Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,198,146 Δ1 bp coding (230/1980 nt) PA14_RS14610 ← cation/acetate symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,198,1440G.99.7% 1860.5 / ‑2.5 380coding (232/1980 nt)PA14_RS14610cation/acetate symporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base . (204/174);  minor base C (1/0);  total (205/175)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 402 total aligned reads displayed.

CGGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAGGGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCAGCAGGAGCA  >  NC_008463/3197994‑3198290
                                                                                                                                                      |                                                                                                                                                  
cgGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAg                                                                                                                                                    >  1:5282126/1‑151 (MQ=255)
 gggCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAgg                                                                                                                                                   <  1:7291384/151‑1 (MQ=255)
 gggCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAgg                                                                                                                                                   >  1:1311548/1‑151 (MQ=255)
 gggCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGAGCGAATTCGCCGTTGGCGCGGTCCACATAGAGCGCCGACAAgg                                                                                                                                                   >  2:6455618/1‑151 (MQ=255)
  ggCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggc                                                                                                                                                  <  1:10245409/151‑2 (MQ=255)
   gCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggca                                                                                                                                                 >  1:5199074/1‑149 (MQ=255)
     gAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcaca                                                                                                                                               >  2:958161/1‑147 (MQ=255)
      aGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACag                                                                                                                                             <  1:3262756/151‑1 (MQ=255)
       ggCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACaga                                                                                                                                            <  2:5061455/151‑1 (MQ=255)
        gCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACagaa                                                                                                                                           <  1:8032086/151‑1 (MQ=255)
           gACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAgaagaa                                                                                                                                        <  2:2660699/151‑1 (MQ=255)
           gACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAgaagaa                                                                                                                                        <  2:5772132/151‑1 (MQ=255)
               gcagGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACg                                                                                                                                    <  1:8466216/151‑1 (MQ=255)
                  ggCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAAc                                                                                                                                 <  1:8253291/151‑1 (MQ=255)
                      ggAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGCACAGGa                                                                                                                             <  2:4044947/151‑1 (MQ=255)
                       gAGTGCAGCGACCACTCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGat                                                                                                                            <  2:8618073/151‑2 (MQ=255)
                       gAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGaa                                                                                                                            >  1:868795/1‑151 (MQ=255)
                         gTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAaca                                                                                                                          >  2:2224433/1‑151 (MQ=255)
                          tGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAacac                                                                                                                         >  1:506404/1‑151 (MQ=255)
                            cAGCGACAACGCCAGGGGAAGGAGCTTCTTCTTGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCTACTGCGCCGTTGGCTCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgc                                                                                                                       <  2:2996725/151‑1 (MQ=255)
                               cGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccgg                                                                                                                    >  2:9171282/1‑151 (MQ=255)
                                gACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccggc                                                                                                                   <  2:4738317/151‑1 (MQ=255)
                                gACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccggc                                                                                                                   >  2:4421007/1‑151 (MQ=255)
                                   aaCGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCcgc                                                                                                                >  2:4991366/1‑151 (MQ=255)
                                   aaCGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCcgc                                                                                                                >  1:3841396/1‑151 (MQ=255)
                                       ccAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGAcc                                                                                                            <  1:1166836/151‑1 (MQ=255)
                                        cAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGAccc                                                                                                           >  1:8943791/1‑151 (MQ=255)
                                         aGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGAcccc                                                                                                          <  1:671469/151‑1 (MQ=255)
                                            ggAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGaa                                                                                                       >  1:2398395/1‑151 (MQ=255)
                                            ggAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGaa                                                                                                       >  1:4048861/1‑151 (MQ=255)
                                             gAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAg                                                                                                      >  2:7505909/1‑151 (MQ=255)
                                             gAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAg                                                                                                      >  2:1000461/1‑151 (MQ=255)
                                              aaGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCTCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAgg                                                                                                     >  2:5342536/1‑151 (MQ=255)
                                               aGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGt                                                                                                    >  1:2820619/1‑151 (MQ=255)
                                                  aGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCAt                                                                                                 >  1:605681/1‑151 (MQ=255)
                                                   gCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATg                                                                                                <  2:978405/151‑1 (MQ=255)
                                                      tcttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTc                                                                                             <  1:5549083/151‑1 (MQ=255)
                                                       cttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCg                                                                                            >  1:7793498/1‑151 (MQ=255)
                                                       cttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCg                                                                                            >  1:3397982/1‑151 (MQ=255)
                                                        ttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGa                                                                                           >  2:9891995/1‑151 (MQ=255)
                                                        ttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGa                                                                                           >  1:2982720/1‑151 (MQ=255)
                                                         tcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAg                                                                                          >  2:10769061/1‑151 (MQ=255)
                                                            tGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGccc                                                                                       <  1:1539416/151‑1 (MQ=255)
                                                             gcgcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCt                                                                                      <  1:4531788/151‑1 (MQ=255)
                                                              cgcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGACTTCGCCGTTGGCGCGGTACACCTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGACCACGCCGGCCGCGACCCCGAAGGTCAGGTTCGAGCCCTg                                                                                     >  2:10256865/1‑151 (MQ=255)
                                                              cgcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTg                                                                                     >  1:2583648/1‑151 (MQ=255)
                                                               gcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgc                                                                                    >  1:4815271/1‑151 (MQ=255)
                                                                    gCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCCTGTTCGAGCCCTGCGCCAg                                                                               <  2:3479350/151‑1 (MQ=255)
                                                                    gCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAg                                                                               <  2:6597500/151‑1 (MQ=255)
                                                                       cctcTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACg                                                                            >  1:3629418/1‑151 (MQ=255)
                                                                         tctTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGACGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGcc                                                                          >  2:8695782/1‑151 (MQ=255)
                                                                          ctTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCt                                                                         <  2:3181075/151‑1 (MQ=255)
                                                                            tCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTgg                                                                       >  2:6772396/1‑151 (MQ=255)
                                                                            tCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTgg                                                                       <  1:6216188/151‑1 (MQ=255)
                                                                             ccagcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGc                                                                      <  2:1912562/151‑1 (MQ=255)
                                                                                  ccagcGACCAGAGCATGGGTCATGCGATCGAATTCGCCGTTGGCTCGGTACATTGAGAGGTCCGACTA‑GGCACAGAAGATGAAGAACAGTTTCACGACGGCAGCGACCCCGAATGTCATGTTCGAGCCCTGCGCCAGAAGCCTGGCAATCa                                                                 <  2:6127542/151‑1 (MQ=255)
                                                                                   cagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAg                                                                <  1:1038407/151‑1 (MQ=255)
                                                                                        aCCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCgg                                                           <  2:5644257/151‑1 (MQ=255)
                                                                                          cAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCgggg                                                         >  1:201682/1‑151 (MQ=255)
                                                                                          cAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCgggg                                                         >  1:10618327/1‑151 (MQ=255)
                                                                                           agagCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGc                                                        <  2:7697255/151‑1 (MQ=255)
                                                                                            gagCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACAGAGAGCGACGACAA‑GGCACAGAACAACACGACCAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCATGCGCCAGACGCCTGGCAATCAGCTCGGGGCa                                                       <  2:5901438/151‑1 (MQ=255)
                                                                                            gagCCTGGGTCAGGCGAACGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCa                                                       <  2:10197662/151‑1 (MQ=255)
                                                                                               ccTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGaa                                                    <  2:8358668/151‑1 (MQ=255)
                                                                                                   ggTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGAt                                                >  1:4426468/1‑151 (MQ=255)
                                                                                                    gTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATa                                               <  1:9611041/151‑1 (MQ=255)
                                                                                                          cGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAgcg                                         <  2:495242/151‑1 (MQ=255)
                                                                                                           gATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAgcgc                                        >  2:2045922/1‑151 (MQ=255)
                                                                                                             tCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGa                                      <  1:6141986/151‑1 (MQ=255)
                                                                                                             tCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGa                                      >  1:10092965/1‑151 (MQ=255)
                                                                                                                aaTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGt                                   <  2:10148594/151‑1 (MQ=255)
                                                                                                                aaTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGt                                   >  2:2453747/1‑151 (MQ=255)
                                                                                                                 aTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTa                                  >  1:6962173/1‑151 (MQ=255)
                                                                                                                 aTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTa                                  <  1:4590956/151‑1 (MQ=255)
                                                                                                                  ttCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGCACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAg                                 <  2:10609319/151‑1 (MQ=255)
                                                                                                                  ttCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAg                                 <  2:2962984/151‑1 (MQ=255)
                                                                                                                   tCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGc                                >  1:7151067/1‑151 (MQ=255)
                                                                                                                       cGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTg                            >  2:6400516/1‑151 (MQ=255)
                                                                                                                        gTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTgg                           <  1:5755900/151‑1 (MQ=255)
                                                                                                                         ttGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAATGATACCAGCGCGATGTAGCCGTGGa                          <  2:10544791/151‑1 (MQ=255)
                                                                                                                           ggCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAAc                        >  2:2928894/1‑151 (MQ=255)
                                                                                                                            gcgcGGTACATATAGAGCGCCGTCAAGGG‑TCTGTAGTACTCGCACAGGTAGTCGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACg                       <  2:7208825/151‑1 (MQ=255)
                                                                                                                             cgcgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGt                      >  2:5987535/1‑151 (MQ=255)
                                                                                                                              gcgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCACTCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTg                     <  2:7039316/151‑1 (MQ=255)
                                                                                                                              gcgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTg                     >  2:7815017/1‑151 (MQ=255)
                                                                                                                              gcgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTg                     <  2:10420521/151‑1 (MQ=255)
                                                                                                                               cgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTgg                    <  1:5384636/151‑1 (MQ=255)
                                                                                                                                ggTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGc                   >  2:1746047/1‑151 (MQ=255)
                                                                                                                                  tACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCa                 >  1:7027214/1‑151 (MQ=255)
                                                                                                                                     acaTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGa              <  1:9171282/151‑1 (MQ=255)
                                                                                                                                      caTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGAt             >  2:4230762/1‑151 (MQ=255)
                                                                                                                                      caTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGAt             <  2:1490797/151‑1 (MQ=255)
                                                                                                                                       aTAGAGCGCCGACAA‑GGCACAGCAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATc            <  2:8139489/151‑1 (MQ=255)
                                                                                                                                       aTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATc            >  2:10498602/1‑151 (MQ=255)
                                                                                                                                        tAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATca           >  2:2109687/1‑151 (MQ=255)
                                                                                                                                        tAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATca           >  1:9420263/1‑151 (MQ=255)
                                                                                                                                              gcCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcagga     <  2:7833139/151‑1 (MQ=255)
                                                                                                                                              gcCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcagga     <  1:6382259/151‑1 (MQ=255)
                                                                                                                                              gcCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcagga     <  1:975142/151‑1 (MQ=255)
                                                                                                                                               ccGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcaggag    >  2:3659530/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
CGGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAGGGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCAGCAGGAGCA  >  NC_008463/3197994‑3198290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑