Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,700,807 Δ1 bp intergenic (+165/‑150) PA14_RS21585 → / → pbpG aspartate/tyrosine/aromatic aminotransferase/D‑alanyl‑D‑alanine endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,700,8070G.100.0% 2246.1 / NA 454intergenic (+165/‑150)PA14_RS21585/pbpGaspartate/tyrosine/aromatic aminotransferase/D‑alanyl‑D‑alanine endopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (182/272);  total (182/272)

Only 100 of 454 total aligned reads displayed.

AGCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGCGCGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCAAC  >  NC_008463/4700661‑4700952
                                                                                                                                                  |                                                                                                                                                 
 gCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAAcc                                                                                                                                             >  1:1972747/1‑151 (MQ=255)
     gCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCtctc                                                                                                                                         <  2:9292455/151‑1 (MQ=255)
     gCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCtctc                                                                                                                                         >  2:8707444/1‑151 (MQ=255)
      cTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTcc                                                                                                                                        <  1:9178764/151‑1 (MQ=255)
        gtgATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGa                                                                                                                                      >  1:7117168/1‑151 (MQ=255)
          gATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAAc                                                                                                                                    <  2:1872644/151‑1 (MQ=255)
           aTGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGATATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAAcc                                                                                                                                   <  2:1669909/151‑1 (MQ=255)
                 ggCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCa                                                                                                                             <  1:8569840/151‑1 (MQ=255)
                    tttttttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAAcc                                                                                                                          >  2:8549449/1‑151 (MQ=255)
                         ttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAg                                                                                                                     <  2:1046922/151‑1 (MQ=255)
                          tCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGt                                                                                                                    <  2:8325486/151‑1 (MQ=255)
                           cGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTACCAACCATAAGTc                                                                                                                   <  1:6953463/151‑1 (MQ=255)
                                 aGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATCAGTCAGCACt                                                                                                             >  1:10140460/1‑151 (MQ=255)
                                 aGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACt                                                                                                             >  1:2323162/1‑151 (MQ=255)
                                  gCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACtt                                                                                                            <  2:7470289/151‑1 (MQ=255)
                                   cGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACttt                                                                                                           <  1:5006659/151‑1 (MQ=255)
                                   cGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCGCCGAACCGAGCCAACCATAAGTCAGCACttt                                                                                                           >  2:4784117/1‑151 (MQ=255)
                                    gAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTc                                                                                                          >  1:8405932/1‑151 (MQ=255)
                                    gAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTc                                                                                                          >  2:1353877/1‑151 (MQ=255)
                                       tGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCaaa                                                                                                       >  2:559487/1‑151 (MQ=255)
                                       tGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCaaa                                                                                                       <  2:2223545/151‑1 (MQ=255)
                                        gCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAg                                                                                                      >  1:4292709/1‑151 (MQ=255)
                                        gCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAg                                                                                                      >  2:3288571/1‑151 (MQ=255)
                                         ccGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGc                                                                                                     <  2:6749586/151‑1 (MQ=255)
                                         ccGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGc                                                                                                     <  1:7289167/151‑1 (MQ=255)
                                           gcgcACTCCCGCCGAGGCGGCGGGTAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGccc                                                                                                   <  1:4065641/151‑1 (MQ=255)
                                           gcgcACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGccc                                                                                                   <  2:7723357/151‑1 (MQ=255)
                                             gcACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTa                                                                                                 <  2:9201888/151‑1 (MQ=255)
                                             gcACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTa                                                                                                 <  2:6407401/151‑1 (MQ=255)
                                              cACTCCCGCCGTGGCGGCGGATACCCGCGTTGCGGTTATTCGCCCGACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGTACCTCTCCGAACCGTGCCAACCATACGTCAGCAGGGTCTAAGCCCTag                                                                                                >  2:8954234/1‑150 (MQ=255)
                                              cACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTAc                                                                                                <  2:1910013/151‑1 (MQ=255)
                                                 tCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCCCCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTg                                                                                             <  1:6608770/151‑1 (MQ=255)
                                                  cccGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGGTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCCTAAGTCAGCACTTTCAAAGCCCTACGTGc                                                                                            >  1:7370434/1‑151 (MQ=255)
                                                   ccgccgTGGCGGCGGATAACCGCGTTGCGGTTTTTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCt                                                                                           <  1:9381451/151‑1 (MQ=255)
                                                   ccgccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCt                                                                                           <  2:3478451/151‑1 (MQ=255)
                                                      ccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCtttt                                                                                        <  2:6860750/151‑1 (MQ=255)
                                                      ccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCtttt                                                                                        <  2:10062991/151‑1 (MQ=255)
                                                      ccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCtttt                                                                                        >  2:9742961/1‑151 (MQ=255)
                                                       cgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTc                                                                                       <  2:4055/151‑1 (MQ=255)
                                                        gAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTcc                                                                                      <  2:4252287/151‑1 (MQ=255)
                                                          ggcggcGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTa                                                                                    <  2:7005477/151‑1 (MQ=255)
                                                             ggcggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACaa                                                                                 >  2:8796651/1‑151 (MQ=255)
                                                              gcggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACaaa                                                                                <  2:1984461/151‑1 (MQ=255)
                                                              gcggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACaaa                                                                                >  2:10304041/1‑151 (MQ=255)
                                                               cggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAAACTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACaaaa                                                                               <  1:3472135/151‑1 (MQ=255)
                                                                ggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAag                                                                              <  1:8739822/151‑1 (MQ=255)
                                                                ggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAag                                                                              >  2:6172670/1‑151 (MQ=255)
                                                                ggATAACCGCGGTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAag                                                                              <  2:7248100/151‑1 (MQ=255)
                                                                 gagaaCCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACACAaga                                                                             <  2:1065934/148‑1 (MQ=255)
                                                                  aTAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAagag                                                                            <  2:3078223/151‑1 (MQ=255)
                                                                  aTAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAagag                                                                            <  2:2888868/151‑1 (MQ=255)
                                                                   tAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGc                                                                           <  1:6811041/151‑1 (MQ=255)
                                                                     aCCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCAt                                                                         <  1:8132796/151‑1 (MQ=255)
                                                                      ccGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATa                                                                        <  1:4876804/151‑1 (MQ=255)
                                                                         cgTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATaaaa                                                                     <  1:7709059/151‑1 (MQ=255)
                                                                          gTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAAt                                                                    >  1:9540451/1‑151 (MQ=255)
                                                                          gTTGCGGTTATTCGCACTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAAt                                                                    <  1:2525125/151‑1 (MQ=255)
                                                                            tGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGc                                                                  >  2:240304/1‑151 (MQ=255)
                                                                             gCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCt                                                                 <  1:5407325/151‑1 (MQ=255)
                                                                             gCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCt                                                                 <  2:9480194/151‑1 (MQ=255)
                                                                             gCGGTTATTCGCCCTACCGCACCACGACCGTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCt                                                                 <  1:3660642/151‑1 (MQ=255)
                                                                              cGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTc                                                                <  1:1670215/151‑1 (MQ=255)
                                                                              cGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTc                                                                <  1:9759985/151‑1 (MQ=255)
                                                                                  tATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAAtt                                                            >  2:7292960/1‑151 (MQ=255)
                                                                                  tATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAAtt                                                            <  2:8164876/151‑1 (MQ=255)
                                                                                  tATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAAtt                                                            <  1:9015339/151‑1 (MQ=255)
                                                                                  tATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAAtt                                                            <  2:57533/151‑1 (MQ=255)
                                                                                    ttCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTCCAAAAGAGCATAAAATGCTCAATTAt                                                          <  2:1425912/151‑1 (MQ=255)
                                                                                    ttCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTAt                                                          >  1:10384540/1‑151 (MQ=255)
                                                                                    ttCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTAt                                                          <  1:5918749/151‑1 (MQ=255)
                                                                                      cGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATgg                                                        >  2:5027495/1‑151 (MQ=255)
                                                                                         ccTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCa                                                     >  1:2922180/1‑151 (MQ=255)
                                                                                          cTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCAc                                                    >  2:2543419/1‑151 (MQ=255)
                                                                                           tACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCAcc                                                   >  1:6206336/1‑151 (MQ=255)
                                                                                             ccGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAg                                                 <  2:10151622/151‑1 (MQ=255)
                                                                                              cGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGt                                                <  1:10018645/151‑1 (MQ=255)
                                                                                                caccacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTc                                              <  2:5390044/151‑1 (MQ=255)
                                                                                                  ccacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTcac                                            <  1:4336882/151‑1 (MQ=255)
                                                                                                  ccacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTcac                                            <  1:8050115/151‑1 (MQ=255)
                                                                                                      gACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CTAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCGACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTg                                        <  2:6009804/151‑1 (MQ=255)
                                                                                                      gACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTCCGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTg                                        <  1:56096/151‑1 (MQ=255)
                                                                                                      gACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAAGTATGGGCACCAGTTCACACTg                                        >  1:1373440/1‑151 (MQ=255)
                                                                                                          ttGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGcc                                    >  1:1821819/1‑151 (MQ=255)
                                                                                                              gggCGTAGGGCTAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCTTAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTCCAAATGCGCATAAAATGCTCACTTATGGGCACCAGTTCACACTGCGCCCCAc                                <  2:4408866/151‑1 (MQ=255)
                                                                                                              gggCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACACAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCAc                                <  1:4125026/151‑1 (MQ=255)
                                                                                                               ggCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTGTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACg                               >  2:6209095/1‑151 (MQ=255)
                                                                                                                gCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACgg                              <  2:3875897/151‑1 (MQ=255)
                                                                                                                  gTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGcc                            >  1:6414341/1‑151 (MQ=255)
                                                                                                                   tAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGccc                           <  1:2817646/151‑1 (MQ=255)
                                                                                                                        cGAATACCGCCACAGGCTTTATCCGC‑CGAAACTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCGACGTGCTTTTCCTACAAAAGAGCATAAAAGGCTCAATTATGGTCACCAGTTCACACTGCTCCCCACGGCCCCCACt                      <  1:10679337/151‑1 (MQ=255)
                                                                                                                        cGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACt                      >  1:5149058/1‑151 (MQ=255)
                                                                                                                        cGAATACCGCCACAGGCGTAATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACt                      <  1:7570222/151‑1 (MQ=255)
                                                                                                                          aaTACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACttt                    >  1:3758545/1‑151 (MQ=255)
                                                                                                                           aTACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACGTtc                   <  1:5711029/151‑1 (MQ=255)
                                                                                                                            tACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTtct                  >  2:9653156/1‑151 (MQ=255)
                                                                                                                                 ccACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCAGAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCt             <  2:9011080/151‑1 (MQ=255)
                                                                                                                                  cacaGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCtt            >  2:8027293/1‑151 (MQ=255)
                                                                                                                                        cGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAg      <  2:5163720/151‑1 (MQ=255)
                                                                                                                                         gTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAATATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGc     >  2:10022144/1‑151 (MQ=255)
                                                                                                                                            aTCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCAAc  <  2:4897104/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGCGCGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCAAC  >  NC_008463/4700661‑4700952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑