BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,358,513	Δ1 bp	pseudogene (774/1285 nt)	PA14_RS24540 →	site‑specific integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,358,510	0	T	.	99.7%	594.7 / ‑3.4	290	pseudogene (771/1285 nt)	PA14_RS24540	site‑specific integrase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (144/145); total (144/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

CGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGTTTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATG  >  NC_008463/5358360‑5358656

  aTAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGACCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGttt                                                                                                                                                  >  2:3822693/1‑151 (MQ=14)

  aTAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGttt                                                                                                                                                  <  1:10005399/151‑1 (MQ=31)

  aTAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGttt                                                                                                                                                  <  2:9235964/151‑1 (MQ=31)

     gagCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttccg                                                                                                                                               <  1:4184285/151‑4 (MQ=1)

                                                    tcACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTc                                                                                               >  2:10764559/1‑151 (MQ=1)

                                                     cACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCa                                                                                              >  1:3671930/1‑151 (MQ=11)

                                                      aCCGCACTACAAGATCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCAc                                                                                             <  1:3099404/151‑1 (MQ=2)

                                                      aCCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGGTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTACCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGACCATTCAc                                                                                             >  2:6773652/1‑151 (MQ=2)

                                                        cGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCt                                                                                           >  1:8116170/1‑151 (MQ=11)

                                                        cGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCt                                                                                           <  1:839265/151‑1 (MQ=11)

                                                        cGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCt                                                                                           <  1:9450786/151‑1 (MQ=11)

                                                        cGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCt                                                                                           <  1:4979891/151‑1 (MQ=11)

                                                          cACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCGATGTACCGGATGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCa                                                                                         <  2:6738258/151‑1 (MQ=2)

                                                                acgagCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGt                                                                                   <  2:5557263/149‑1 (MQ=11)

                                                                aaGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGt                                                                                   >  1:601579/1‑151 (MQ=11)

                                                                  gagCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGTGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGc                                                                                 <  1:1243095/151‑1 (MQ=2)

                                                                   agCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGcc                                                                                <  1:5215077/151‑1 (MQ=11)

                                                                     cAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTg                                                                              >  1:1394593/1‑151 (MQ=11)

                                                                     cAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTg                                                                              <  2:5764755/151‑1 (MQ=11)

                                                                      aaGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGc                                                                             >  1:8962923/1‑151 (MQ=11)

                                                                         gTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCa                                                                          >  2:1523859/1‑151 (MQ=11)

                                                                         gTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCa                                                                          >  1:4439153/1‑151 (MQ=11)

                                                                           gatgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAAc                                                                        <  2:8465928/151‑1 (MQ=11)

                                                                           gatgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAAc                                                                        >  1:9815577/1‑151 (MQ=11)

                                                                               aaCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCCCt                                                                    >  2:7996324/1‑151 (MQ=2)

                                                                                aCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTc                                                                   <  1:3389892/151‑1 (MQ=11)

                                                                                aCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTc                                                                   <  2:271396/151‑1 (MQ=11)

                                                                                 cTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCg                                                                  <  2:4325856/151‑1 (MQ=11)

                                                                                    cGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAat                                                               >  2:1373134/1‑151 (MQ=11)

                                                                                    cGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAat                                                               <  1:9014183/151‑1 (MQ=11)

                                                                                    cGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAat                                                               <  1:25659/151‑1 (MQ=11)

                                                                                    cGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGAGGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTGGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAat                                                               >  2:1368801/1‑151 (MQ=2)

                                                                                     gACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAata                                                              >  1:3634388/1‑151 (MQ=11)

                                                                                     gACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAata                                                              >  1:8292085/1‑151 (MQ=11)

                                                                                        cAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATcc                                                           <  1:5842041/151‑1 (MQ=11)

                                                                                          ggTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAg                                                         <  1:10105207/151‑1 (MQ=11)

                                                                                            tCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGAc                                                       <  2:8566381/151‑1 (MQ=11)

                                                                                             cGAAGCCATGTACCGGAAGTTGACCGAGCAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGTACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGAcc                                                      >  2:5076473/1‑151 (MQ=2)

                                                                                             cGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGAcc                                                      <  2:181874/151‑1 (MQ=11)

                                                                                             cGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGAcc                                                      <  1:5470747/151‑1 (MQ=11)

                                                                                              gAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGAccc                                                     >  2:5841965/1‑150 (MQ=11)

                                                                                              gAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCa                                                     >  1:1745693/1‑151 (MQ=11)

                                                                                               aaGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCAc                                                    <  1:3151171/151‑1 (MQ=11)

                                                                                                 gCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGACCATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGa                                                  >  1:4400133/1‑151 (MQ=9)

                                                                                                  ccATGTACCGGAAGTTGACCGAGAAGGGTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGTAGTGCCTGCTCAACCACTCGAATATCCAGACCACGAt                                                 >  2:8085669/1‑151 (MQ=12)

                                                                                                    aTGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGa                                               >  2:2867594/1‑151 (MQ=17)

                                                                                                       tACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCt                                            <  1:5663085/151‑1 (MQ=17)

                                                                                                         ccGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTAc                                          <  1:6427880/151‑1 (MQ=17)

                                                                                                           ggAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACAt                                        <  1:3803887/151‑1 (MQ=17)

                                                                                                            gAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATc                                       <  2:7527154/151‑1 (MQ=17)

                                                                                                            gAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACACGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATc                                       <  2:2585544/151‑1 (MQ=12)

                                                                                                             aaGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCg                                      >  1:8512399/1‑151 (MQ=17)

                                                                                                             aaGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCg                                      >  1:7099268/1‑151 (MQ=17)

                                                                                                              cgTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGCAGTGCCAGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGa                                     <  1:3370688/150‑1 (MQ=255)

                                                                                                              aGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACCTCGa                                     <  2:2359624/151‑1 (MQ=12)

                                                                                                              aGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGa                                     <  1:9916065/151‑1 (MQ=17)

                                                                                                              aGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGa                                     <  2:3912585/151‑1 (MQ=17)

                                                                                                              aGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGa                                     >  2:4353392/1‑151 (MQ=17)

                                                                                                               gTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAg                                    <  2:9696593/151‑1 (MQ=17)

                                                                                                                ttGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAgg                                   <  2:2939687/151‑1 (MQ=17)

                                                                                                                 tGACCGCGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGTTGAAGGCACCCGAGCGGACCATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGc                                  >  2:9097124/1‑151 (MQ=255)

                                                                                                                 tGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGc                                  >  1:7461991/1‑151 (MQ=17)

                                                                                                                 tGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGc                                  >  2:3446953/1‑151 (MQ=17)

                                                                                                                 tGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGc                                  <  1:4676400/151‑1 (MQ=17)

                                                                                                                 tGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGc                                  >  1:9081887/1‑151 (MQ=17)

                                                                                                                  gACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGcc                                 >  2:8380326/1‑151 (MQ=17)

                                                                                                                  gACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGcc                                 >  2:7715216/1‑151 (MQ=17)

                                                                                                                  gACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTCCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGcc                                 >  2:4956889/1‑151 (MQ=12)

                                                                                                                   cccGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCg                                <  2:10108554/150‑1 (MQ=17)

                                                                                                                    ccGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGa                               <  2:702651/151‑1 (MQ=17)

                                                                                                                    ccGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGa                               <  2:5880864/151‑1 (MQ=17)

                                                                                                                    ccGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGa                               >  1:6592264/1‑151 (MQ=17)

                                                                                                                    ccGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGa                               <  1:3242275/151‑1 (MQ=17)

                                                                                                                    ccGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGa                               <  1:10749103/151‑1 (MQ=17)

                                                                                                                     cGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCCCTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGAc                              <  2:6421972/151‑1 (MQ=12)

                                                                                                                      gagaCGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACt                             <  2:6098212/151‑1 (MQ=12)

                                                                                                                      gagaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACt                             >  1:2259778/1‑151 (MQ=17)

                                                                                                                      gagaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACt                             >  1:7679045/1‑151 (MQ=17)

                                                                                                                      gagaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACt                             >  1:10245919/1‑151 (MQ=17)

                                                                                                                       agaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTa                            >  2:3152972/1‑151 (MQ=17)

                                                                                                                           ggTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGAt                        >  1:6195744/1‑151 (MQ=18)

                                                                                                                           ggTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACAGTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGAt                        >  2:222/1‑151 (MQ=16)

                                                                                                                             ttGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATca                      <  1:7611913/151‑1 (MQ=21)

                                                                                                                              tGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCTCTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATcac                     >  2:8829462/1‑151 (MQ=19)

                                                                                                                               ggggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATcaca                    <  1:4394169/151‑1 (MQ=21)

                                                                                                                                gggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACAt                   <  1:7511200/151‑1 (MQ=21)

                                                                                                                                gggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACAt                   >  1:9538758/1‑151 (MQ=21)

                                                                                                                                gggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACAt                   <  2:4617010/151‑1 (MQ=21)

                                                                                                                                 ggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATg                  <  2:6907515/151‑1 (MQ=21)

                                                                                                                                 ggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATg                  >  2:6532728/1‑151 (MQ=21)

                                                                                                                                 ggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATg                  >  1:3178410/1‑151 (MQ=21)

                                                                                                                                   tGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcg                <  2:4232508/151‑1 (MQ=21)

                                                                                                                                     cGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtg              >  1:6751698/1‑151 (MQ=21)

                                                                                                                                     cGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtg              >  1:7913606/1‑151 (MQ=21)

                                                                                                                                           aCCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgccgtgc        >  2:9583011/1‑151 (MQ=21)

                                                                                                                                            ccccGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGACTATCCAGACCACGATGAGCTCCATCGAGGCCGACTACGATCACATGCGTGCCGtgct       >  2:10218294/1‑151 (MQ=255)

                                                                                                                                            ccccGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGtgct       >  1:5096842/1‑151 (MQ=18)

                                                                                                                                             cccGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGtgctg      <  1:555766/151‑1 (MQ=18)

                                                                                                                                                gCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCAt   >  2:10504986/1‑151 (MQ=17)

                                                                                                                                                gCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCAt   <  2:2831999/151‑1 (MQ=17)

CGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGTTTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATG  >  NC_008463/5358360‑5358656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`