Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,354,163 (G)5→4 intergenic (‑48/+3) PA14_RS10945 ← / ← lipA lipase secretion chaperone/triacylglycerol lipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,354,1590G.100.0% 1939.7 / NA 391intergenic (‑44/+7)PA14_RS10945/lipAlipase secretion chaperone/triacylglycerol lipase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (206/185);  total (206/185)

Only 100 of 406 total aligned reads displayed.

GCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAAGCCCCGGCCGGGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCACCATGC  >  NC_008463/2354009‑2354305
                                                                                                                                                      |                                                                                                                                                  
     ggCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCCggggtc                                                                                                                                               >  1:10773560/1‑149 (MQ=255)
        cGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTa                                                                                                                                           >  1:8609479/1‑151 (MQ=255)
        cGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTa                                                                                                                                           >  2:9082465/1‑151 (MQ=255)
            ggCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAgg                                                                                                                                       >  1:1043466/1‑151 (MQ=255)
              cgcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCt                                                                                                                                     <  2:1675513/151‑1 (MQ=255)
               gcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGACCGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTg                                                                                                                                    <  1:9169511/151‑1 (MQ=255)
                 cGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGc                                                                                                                                  >  2:9460794/1‑151 (MQ=255)
                  gTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCg                                                                                                                                 >  1:2627793/1‑151 (MQ=255)
                  gTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCGGGCGGCGACCGCAAGTGGAATCAGCAGGAGGATTTTCTTCACGCTAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCg                                                                                                                                 <  1:4710066/151‑1 (MQ=255)
                     tcGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttc                                                                                                                              >  1:1270409/1‑151 (MQ=255)
                       gggTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttctt                                                                                                                            >  1:4163130/1‑151 (MQ=255)
                       gggTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttctt                                                                                                                            >  1:7131500/1‑151 (MQ=255)
                       gggTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGtgctt                                                                                                                            >  1:3278798/1‑151 (MQ=255)
                        ggTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCTGGCTGGCGGCGATCGCCAGTGGAATCAGCAGGAGGATTTTCTTCCCGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttcttc                                                                                                                           <  2:3056089/151‑1 (MQ=255)
                         gcgCGGGGTATGGTTCCAGCCAGACGTACCAGGCCAGGCTGGCGGAGAACGCCAGTGGACTCAGCAGGAGGTTTTTCTTCACGCTAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTTCAGGCTGGCGTTCTTCa                                                                                                                          <  1:6265248/149‑1 (MQ=255)
                          tGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCCCGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAg                                                                                                                         <  1:3519304/151‑1 (MQ=255)
                          tGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAg                                                                                                                         >  1:8102623/1‑151 (MQ=255)
                           gCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAgg                                                                                                                        >  1:8286679/1‑151 (MQ=255)
                             gggggAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCg                                                                                                                      >  2:8080280/1‑151 (MQ=255)
                                ggAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGtt                                                                                                                   <  2:4570831/151‑1 (MQ=255)
                                 gatggTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTg                                                                                                                  <  1:5410898/148‑1 (MQ=255)
                                 gAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGACAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTg                                                                                                                  >  2:4673090/1‑151 (MQ=255)
                                  aaGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTgg                                                                                                                 >  1:5636308/1‑151 (MQ=255)
                                   aGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGc                                                                                                                <  1:10188835/151‑1 (MQ=255)
                                     gTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGt                                                                                                              >  1:3100882/1‑151 (MQ=255)
                                         cagccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACGGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTg                                                                                                          <  2:6871428/151‑1 (MQ=255)
                                             cagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAGGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCgg                                                                                                      >  2:4606376/1‑151 (MQ=255)
                                              agACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGt                                                                                                     >  1:4755936/1‑151 (MQ=255)
                                              agACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGt                                                                                                     >  2:7953308/1‑151 (MQ=255)
                                               gACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTa                                                                                                    >  1:6408233/1‑151 (MQ=255)
                                                 cGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGCGGGGGCTTCCGGGAAAGGGCCGGGGCCGCGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGa                                                                                                  <  1:8260920/151‑1 (MQ=255)
                                                 cGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGACAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGa                                                                                                  >  1:10125885/1‑151 (MQ=255)
                                                    aCCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGc                                                                                               >  1:9632156/1‑151 (MQ=255)
                                                     ccaggccaggCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCt                                                                                              >  1:8171144/1‑151 (MQ=255)
                                                     ccaggccaggCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCt                                                                                              <  1:3609951/151‑1 (MQ=255)
                                                      caggccaggcTGGCGGCGAACGCCAGTGGAAACAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTg                                                                                             <  2:6763623/151‑1 (MQ=255)
                                                       aggccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGa                                                                                            >  1:1700738/1‑151 (MQ=255)
                                                       aggccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGa                                                                                            >  2:7526067/1‑151 (MQ=255)
                                                        ggccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGTCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGAc                                                                                           <  2:3407932/151‑1 (MQ=255)
                                                        ggccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGAc                                                                                           >  1:7212409/1‑151 (MQ=255)
                                                           caggcTGGCGGCGAACGCCAGGGGAATCAGCAGGAGGAGTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCGTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCgg                                                                                        >  2:10458873/1‑151 (MQ=255)
                                                            aggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCGGGCGGTAGACGCTGACCggg                                                                                       <  1:6619526/151‑1 (MQ=255)
                                                             ggcTGGCGGCGAACGCCAGTGGCAGCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAATGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGGGCTTCAGGCGGGTGGCGTGCTGGCGGGGGACGCTGAGCGGGc                                                                                      >  2:9111378/1‑151 (MQ=255)
                                                              gcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCt                                                                                     <  1:4971866/151‑1 (MQ=255)
                                                               cTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTg                                                                                    >  1:236727/1‑151 (MQ=255)
                                                                tGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTgg                                                                                   >  2:327056/1‑151 (MQ=255)
                                                                 ggcggcGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGt                                                                                  >  1:9920334/1‑151 (MQ=255)
                                                                   cggcgAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGtct                                                                                <  2:3022048/151‑1 (MQ=255)
                                                                    ggcgAACGCCAGTGGAATCAGCAGGAGTATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGtctc                                                                               <  1:2686567/151‑1 (MQ=255)
                                                                    ggcgAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGtctc                                                                               >  2:1004695/1‑151 (MQ=255)
                                                                      cgAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGa                                                                             >  2:1912541/1‑151 (MQ=255)
                                                                       gAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGaa                                                                            >  2:3614111/1‑151 (MQ=255)
                                                                        accGCCAGTGGACTCAGCAGGAGGATTTTCTTCACGCGAGGGGGCGTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAAc                                                                           <  2:9270149/149‑1 (MQ=255)
                                                                           gCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAgg                                                                        <  2:5272168/151‑1 (MQ=255)
                                                                           gCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAgg                                                                        >  2:5357242/1‑151 (MQ=255)
                                                                           gCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAgg                                                                        <  2:10073606/151‑1 (MQ=255)
                                                                            ccAGTGGAATCAGCAGGCGGATTTTCGTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCGACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGc                                                                       <  2:2713434/151‑1 (MQ=255)
                                                                             cAGTGGAATCAGCCGGAGGATTTTCGTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCt                                                                      <  1:3727453/151‑1 (MQ=255)
                                                                                tGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGt                                                                   <  1:1795037/151‑1 (MQ=255)
                                                                                 ggAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTg                                                                  <  2:8405517/151‑1 (MQ=255)
                                                                                  gactCAGCAGGAGGATTCTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGa                                                                 <  2:3830067/148‑1 (MQ=255)
                                                                                  gAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGa                                                                 >  1:6052260/1‑151 (MQ=255)
                                                                                      cagcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGcc                                                             <  2:710467/151‑1 (MQ=255)
                                                                                      cagcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGcc                                                             <  1:7661264/151‑1 (MQ=255)
                                                                                      cagcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGcc                                                             >  1:9811880/1‑151 (MQ=255)
                                                                                          aggaggATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAg                                                         >  2:8568484/1‑151 (MQ=255)
                                                                                           ggaggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGa                                                        <  2:115655/151‑1 (MQ=255)
                                                                                           ggaggaTTTTCGTCACGCGAGGGGGCTTGCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGa                                                        <  2:7315544/151‑1 (MQ=255)
                                                                                            gaggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGAc                                                       <  1:1372003/151‑1 (MQ=255)
                                                                                            gaggaTTGTCTTCACGCGCGGGGGCTACCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGAc                                                       <  1:7429366/151‑1 (MQ=255)
                                                                                             aggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGAcc                                                      <  1:5153604/151‑1 (MQ=255)
                                                                                                  tttCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAACACCTGGtt                                                 >  1:10347375/1‑151 (MQ=255)
                                                                                                   ttcttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTc                                                >  1:1492807/1‑151 (MQ=255)
                                                                                                     cttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCAGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCAc                                              <  2:4347492/151‑1 (MQ=255)
                                                                                                       tcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCt                                            <  1:2564185/151‑1 (MQ=255)
                                                                                                       tcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCt                                            <  2:1885710/151‑1 (MQ=255)
                                                                                                                  ggCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAggtgg                                 >  1:2437700/1‑151 (MQ=255)
                                                                                                                   gCTTCCGGTAAAGGTCCTGGGCCGAGGCACCTGAC‑GGGTTCCTAAAGGCTGGCGTTCTTCTGGCGGTTGGCTTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGTCCGAAGACCTGGTTCACCTCGTCCAggtggt                                >  2:504827/1‑151 (MQ=255)
                                                                                                                     ttCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTc                              >  2:5031099/1‑151 (MQ=255)
                                                                                                                     ttCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTc                              <  1:5863470/151‑1 (MQ=255)
                                                                                                                        cGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATc                           >  1:8024598/1‑151 (MQ=255)
                                                                                                                         gggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATcc                          >  1:10040260/1‑151 (MQ=255)
                                                                                                                         gggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATcc                          <  1:3959302/151‑1 (MQ=255)
                                                                                                                          ggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCg                         <  2:9716758/151‑1 (MQ=255)
                                                                                                                          ggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTGCATCCg                         >  2:1299490/1‑151 (MQ=255)
                                                                                                                           gAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCgg                        >  1:8871484/1‑151 (MQ=255)
                                                                                                                           gAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCgg                        >  2:10612473/1‑151 (MQ=255)
                                                                                                                                 gccggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTg                  <  2:2274531/151‑1 (MQ=255)
                                                                                                                                 gccggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTg                  >  2:7268191/1‑151 (MQ=255)
                                                                                                                                 gccggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTg                  >  2:6298146/1‑151 (MQ=255)
                                                                                                                                   cggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTc                <  1:2251843/151‑1 (MQ=255)
                                                                                                                                     gggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTcgc              <  1:6744379/151‑1 (MQ=255)
                                                                                                                                        ccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGa           <  2:8731173/151‑1 (MQ=255)
                                                                                                                                        ccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGa           >  2:4790852/1‑151 (MQ=255)
                                                                                                                                         cgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGAt          <  2:8941345/151‑1 (MQ=255)
                                                                                                                                         cgAGGCCCCGGCC‑GGGGTCCTACAGGCGGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGAt          <  2:8799045/151‑1 (MQ=255)
                                                                                                                                          gaggCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATc         >  2:6495826/4‑151 (MQ=255)
                                                                                                                                          gaggCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATc         >  2:5620715/4‑151 (MQ=255)
                                                                                                                                              ccccGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCACCa     >  2:2485091/1‑151 (MQ=255)
                                                                                                                                                 cggcc‑ggGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCACCATGc  <  2:10275705/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAAGCCCCGGCCGGGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCACCATGC  >  NC_008463/2354009‑2354305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑