Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_008463 5,131,729 Δ34 bp pseudogene (288‑321/1180 nt) PA14_RS23490 ← acyl‑CoA dehydrogenase family protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_008463 5131729 5131762 34 603 [0] [0] 599 PA14_RS23490 acyl‑CoA dehydrogenase family protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_008463 = 51317280 (0.000)512 (1.180) 212/264 0.0 100% pseudogene (322/1180 nt) PA14_RS23490 acyl‑CoA dehydrogenase family protein
?NC_008463 5131763 = 0 (0.000)pseudogene (287/1180 nt) PA14_RS23490 acyl‑CoA dehydrogenase family protein

Only 100 of 603 total aligned reads displayed.

TACCGCCGTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGGCGCAGCGATGTCCGAATGTAGGGCGCAGCGATGTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCG  >  NC_008463/5131578‑5131877
                                                                                                                                                      |                                                                                                                                                     
 aCCGCCGTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGt                                                                                                                                                      >  1:2003795‑M1/1‑150 (MQ=255)
  ccgccgTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtc                                                                                                                                                     <  2:5086928‑M1/151‑3 (MQ=255)
     ccgTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccga                                                                                                                                                  <  2:7196833‑M1/151‑6 (MQ=255)
      cgTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTCGTGGAGGATGTAGGGCGCAGCGATGtccgat                                                                                                                                                 >  2:3173179‑M1/1‑145 (MQ=255)
        tGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatg                                                                                                                                               >  2:3305965‑M1/1‑143 (MQ=255)
        tGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatg                                                                                                                                               >  2:4252019‑M1/1‑143 (MQ=255)
         ggTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgc                                                                                                                                              <  1:7087107‑M1/151‑10 (MQ=255)
         ggTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgc                                                                                                                                              <  1:9646995‑M1/151‑10 (MQ=255)
         ggTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgc                                                                                                                                              <  1:4208817‑M1/151‑10 (MQ=255)
         ggTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgc                                                                                                                                              <  2:6991707‑M1/151‑10 (MQ=255)
          gTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgca                                                                                                                                             >  1:3381455‑M1/1‑141 (MQ=255)
           tCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcag                                                                                                                                            <  2:2040507‑M1/151‑12 (MQ=255)
           tCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcag                                                                                                                                            <  2:1136782‑M1/151‑12 (MQ=255)
            cTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagg                                                                                                                                           >  1:2493446‑M1/1‑139 (MQ=255)
            cTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagg                                                                                                                                           <  1:6180619‑M1/151‑13 (MQ=255)
              tCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGAGACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcaggga                                                                                                                                         <  2:2697937‑M1/151‑15 (MQ=255)
                 ccccTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaa                                                                                                                                      >  1:8135851‑M1/1‑134 (MQ=255)
                 ccccTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGAGGGCCGCGACCAACTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaa                                                                                                                                      <  1:799571‑M1/151‑18 (MQ=255)
                       gCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgat                                                                                                                                <  1:6457650‑M1/151‑24 (MQ=255)
                       gCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgat                                                                                                                                <  1:5978132‑M1/151‑24 (MQ=255)
                       gCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgat                                                                                                                                <  2:10147390‑M1/151‑24 (MQ=255)
                         aGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcc                                                                                                                              <  1:7678132‑M1/151‑26 (MQ=255)
                         aGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcc                                                                                                                              <  2:8542362‑M1/151‑26 (MQ=255)
                           gTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccg                                                                                                                            >  1:2416622‑M1/1‑124 (MQ=255)
                           gTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccg                                                                                                                            <  1:1681797‑M1/151‑28 (MQ=255)
                             cGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggt                                                                                                                          <  1:3169274‑M1/151‑30 (MQ=255)
                                aaCCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcag                                                                                                                       >  2:1072384‑M1/1‑119 (MQ=255)
                                aaCCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcag                                                                                                                       <  1:947387‑M1/151‑33 (MQ=255)
                                   cGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggcc                                                                                                                    <  1:5481432‑M1/151‑36 (MQ=255)
                                   cGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGAGGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggcc                                                                                                                    >  2:9172463‑M1/1‑116 (MQ=255)
                                    ggCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccg                                                                                                                   >  2:5808376‑M1/1‑115 (MQ=255)
                                     gCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccgg                                                                                                                  <  1:8392975‑M1/151‑38 (MQ=255)
                                       ccccGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcc                                                                                                                <  2:8078425‑M1/151‑40 (MQ=255)
                                       ccccGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcc                                                                                                                >  2:6039618‑M1/1‑112 (MQ=255)
                                        cccGGCTCGGTCATGGCGAAGGCCGCGACCATCTCGACGGAAACCAGCTTCGGCAGGTACTTGAGCTTCAGCGCCTCGCTGCCGTAGAGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcacggtcaggccgcccc                                                                                                               >  1:10194003‑M1/1‑111 (MQ=255)
                                         ccGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgactgcagggagaaaccgatcccggtcaggccggcccg                                                                                                              >  1:5714080‑M1/1‑110 (MQ=255)
                                         ccGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccg                                                                                                              >  1:8986003‑M1/1‑110 (MQ=255)
                                             cTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccg                                                                                                          <  1:2208067‑M1/151‑46 (MQ=255)
                                               cGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGGAGTGGAGGATGTCGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgat                                                                                                        >  2:10824716‑M1/1‑104 (MQ=255)
                                                 gTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatct                                                                                                      <  1:8566363‑M1/151‑50 (MQ=255)
                                                  tCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCGTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctc                                                                                                     >  2:1586411‑M1/1‑101 (MQ=255)
                                                     tGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttc                                                                                                  >  1:3614274‑M1/1‑98 (MQ=255)
                                                        cGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgat                                                                                               <  2:5589093‑M1/151‑57 (MQ=255)
                                                         gagggccgCGACCATCTCGCCGGATACCAGCTGCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGACGTAGTGGAGGAGGTAGGGCGCAGCGATGtccgaatgcagggagacaccgagcccggtcaggccggcccgaccgatctcttcgatg                                                                                              >  2:180986‑M1/4‑94 (MQ=255)
                                                         gATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatg                                                                                              <  1:5400322‑M1/151‑58 (MQ=255)
                                                           tGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgac                                                                                            <  1:6395580‑M1/151‑60 (MQ=255)
                                                              ccGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccac                                                                                         >  1:8717332‑M1/1‑89 (MQ=255)
                                                               cgcgACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacg                                                                                        >  2:9332875‑M1/1‑88 (MQ=255)
                                                                 cgACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggc                                                                                      >  1:4554061‑M1/1‑86 (MQ=255)
                                                                     cATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctg                                                                                  <  1:10351165‑M1/151‑70 (MQ=255)
                                                                     cATCTCGCCGGAAACCAGCTTCGGCAGGTACGTGCGCGTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctg                                                                                  <  2:10029027‑M1/151‑70 (MQ=255)
                                                                         tcGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtaga                                                                              <  2:5352139‑M1/151‑74 (MQ=255)
                                                                          cGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagag                                                                             >  2:686562‑M1/1‑77 (MQ=255)
                                                                          cGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagag                                                                             <  2:3718270‑M1/151‑75 (MQ=255)
                                                                             cGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgagcacggcgctgtagaggaa                                                                          >  2:5495119‑M1/1‑74 (MQ=255)
                                                                              ggATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaag                                                                         >  2:6318579‑M1/1‑73 (MQ=255)
                                                                               gATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagt                                                                        >  2:6548075‑M1/1‑72 (MQ=255)
                                                                               gATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagt                                                                        <  1:1617998‑M1/151‑80 (MQ=255)
                                                                               gATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagt                                                                        <  1:10045565‑M1/151‑80 (MQ=255)
                                                                                 tACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtcc                                                                      >  2:8920021‑M1/1‑70 (MQ=255)
                                                                                  aCCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccg                                                                     >  1:10497220‑M1/1‑69 (MQ=255)
                                                                                  aCCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccg                                                                     <  2:4656505‑M1/151‑83 (MQ=255)
                                                                                    cAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcg                                                                   >  1:7358901‑M1/1‑67 (MQ=255)
                                                                                     aGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgc                                                                  >  1:5870528‑M1/1‑66 (MQ=255)
                                                                                     aGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgc                                                                  >  2:4415031‑M1/1‑66 (MQ=255)
                                                                                             cAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctggagaggaagtccgcgcccatcccg                                                          >  1:6746908‑M1/1‑58 (MQ=255)
                                                                                              aGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgc                                                         >  1:2663959‑M1/1‑57 (MQ=255)
                                                                                                gTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgcca                                                       >  2:3640213‑M1/1‑55 (MQ=255)
                                                                                                  aCTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccata                                                     >  1:2898647‑M1/1‑53 (MQ=255)
                                                                                                    ttGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatact                                                   >  2:2451084‑M1/1‑51 (MQ=255)
                                                                                                     tGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactc                                                  >  2:9506902‑M1/1‑50 (MQ=255)
                                                                                                     tGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactc                                                  >  1:9245115‑M1/1‑50 (MQ=255)
                                                                                                       cgcTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactctt                                                <  2:4817505‑M1/151‑104 (MQ=255)
                                                                                                          ttCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccg                                             >  2:10506776‑M1/1‑45 (MQ=255)
                                                                                                            cAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgctcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggc                                           <  2:8284557‑M1/151‑109 (MQ=255)
                                                                                                                 ccTCGCGGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgagcccggtcaggccggcccgaccgatcgcttcgatgaccacggcgctggagaggaggtccgcgcccatcccgccatactcttccggcaggtg                                      >  2:2843101‑M1/1‑38 (MQ=255)
                                                                                                                     gCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaaggccgcgcccatcccgccatactcttccggcaggtgcgaa                                  <  1:1895523‑M1/151‑118 (MQ=255)
                                                                                                                      cTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaac                                 >  1:4373774‑M1/1‑33 (MQ=255)
                                                                                                                          cGTAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagag                             <  1:5552996‑M1/151‑123 (MQ=255)
                                                                                                                            tAGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagca                           >  1:7816841‑M1/1‑27 (MQ=255)
                                                                                                                             aGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcat                          <  1:3863284‑M1/151‑126 (MQ=255)
                                                                                                                             aGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcat                          <  2:8799268‑M1/151‑126 (MQ=255)
                                                                                                                             aGTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccagactcttccggcaggtgcgaacagagcat                          <  2:7451146‑M1/151‑126 (MQ=255)
                                                                                                                              gTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcatt                         <  1:10572658‑M1/151‑127 (MQ=255)
                                                                                                                              gTGGAGGATGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcatt                         <  2:4907155‑M1/151‑127 (MQ=255)
                                                                                                                                ggaggaTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattcc                       <  2:7071655‑M1/151‑129 (MQ=255)
                                                                                                                                   ggaTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggc                    >  2:2138349‑M1/1‑20 (MQ=255)
                                                                                                                                    gaTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccctactcttacggcaggtgcgaacagagcattccggcc                   >  1:4909377‑M1/1‑19 (MQ=255)
                                                                                                                                    gaTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcc                   <  1:1269114‑M1/151‑133 (MQ=255)
                                                                                                                                     aTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcct                  <  2:1916452‑M1/151‑134 (MQ=255)
                                                                                                                                     aTGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcct                  >  2:7948894‑M1/1‑18 (MQ=255)
                                                                                                                                      tGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctc                 <  2:1282045‑M1/151‑135 (MQ=255)
                                                                                                                                      tGTAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctc                 >  1:5130078‑M1/1‑17 (MQ=255)
                                                                                                                                        tAGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgc               >  1:1079435‑M1/1‑15 (MQ=255)
                                                                                                                                         aGGGCGCAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgcc              <  2:780327‑M1/151‑138 (MQ=255)
                                                                                                                                               cAGCGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccggcctt        >  2:2304217‑M1/1‑8 (MQ=255)
                                                                                                                                                  cGATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccggccttgtt     >  2:10271218‑M1/1‑5 (MQ=255)
                                                                                                                                                   gATGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccggccttgttc    <  2:7351966‑M1/151‑148 (MQ=255)
                                                                                                                                                    aTGtccgaatgcagggagaaaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccatcccgccatactcttccggcaggtgcgaacagagcattccggcctcgccggccttgttcc   >  1:6612664‑M1/1‑3 (MQ=255)
                                                                                                                                                     ggtccgactgcagggagacaccgatcccggtcaggccggcccgaccgatctcttcgatgaccacggcgctgtagaggaagtccgcgcccaacccgccatactcttccggcaggtgcgaacagagcattccggcctcgccggccttgttcca  <  2:7696538‑M1/150‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TACCGCCGTGGTCTTCACCCCTTGCAGGTCGGAACCGGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGGCGCAGCGATGTCCGAATGTAGGGCGCAGCGATGTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCG  >  NC_008463/5131578‑5131877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑