Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,437,0140AG31.0% 1039.3 / inf 540D102G (GAC→GGC) PA14_RS20265OmpA family protein
Reads supporting (aligned to +/- strand):  ref base A (191/180);  new base G (93/74);  total (284/256)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 544 total aligned reads displayed.

ACGAAGGCTGGGAGTTCGGCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGCCGGTGGCCGATAACAAGACCCG  >  NC_008463/4436864‑4437164
                                                                                                                                                      |                                                                                                                                                      
acgaAGGCTGGGAGTTCGGCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGa                                                                                                                                                        <  2:3338896/151‑1 (MQ=255)
  gaAGGCTGGGAGTTCGGCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACa                                                                                                                                                      <  1:8051913/151‑1 (MQ=255)
  gaAGGCTGGGAGTTCGGCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACa                                                                                                                                                      >  1:5403268/1‑151 (MQ=255)
        tGGGAGTTCGGCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACg                                                                                                                                                <  1:10614468/151‑1 (MQ=255)
        tGGGAGTTCGGCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCACAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACg                                                                                                                                                >  2:1961443/1‑151 (MQ=255)
          ggAGTTCGGCATGTCCAGCAAGCTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGCCGAAGATCGCCCACGCCTTGCTCGCGGTAGACCTCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGc                                                                                                                                              >  2:895022/1‑151 (MQ=255)
           gAGTTCGGCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGcg                                                                                                                                             >  2:4789525/1‑151 (MQ=255)
               tCGGCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGcgacga                                                                                                                                         >  2:4372137/1‑151 (MQ=255)
               tCGGCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGcgacga                                                                                                                                         >  2:4731497/1‑151 (MQ=255)
               tCGCCATGGCCACCAAGGAGCGGTACGGCAACCATCTCGACCGTCTCAAGCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACCAGGTCCGCCTGGCAGGCCACACCGACAACTACGGcgacga                                                                                                                                         <  2:1520055/151‑1 (MQ=255)
                cGGCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGaa                                                                                                                                        <  1:2761019/151‑1 (MQ=255)
                cGGCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGaa                                                                                                                                        <  1:1545099/151‑1 (MQ=255)
                 ggCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCATCACCCTGACGAAGATCGCCAACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAg                                                                                                                                       <  1:7513354/151‑1 (MQ=255)
                 ggCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAg                                                                                                                                       <  1:9433797/151‑1 (MQ=255)
                  gCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAgg                                                                                                                                      <  1:8734909/151‑1 (MQ=255)
                   cATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGc                                                                                                                                     >  1:4867259/1‑151 (MQ=255)
                   cATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGc                                                                                                                                     >  1:6425162/1‑151 (MQ=255)
                   aaTGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCACTCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGc                                                                                                                                     <  2:10144696/150‑1 (MQ=255)
                       tCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACa                                                                                                                                 >  1:3895399/1‑151 (MQ=255)
                         cAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAAc                                                                                                                               >  2:3837972/1‑151 (MQ=255)
                         cAGCAAGGTGCTGTTCGGCAACAATCTCGACCGACTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAAc                                                                                                                               >  1:3812941/1‑151 (MQ=255)
                          aGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAAcc                                                                                                                              >  1:5100334/1‑151 (MQ=255)
                            cAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAg                                                                                                                            >  2:9685546/1‑151 (MQ=255)
                             aaGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGa                                                                                                                           >  1:10787784/1‑151 (MQ=255)
                               ggTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAg                                                                                                                         <  2:1721348/151‑1 (MQ=255)
                                  gCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGctc                                                                                                                      >  1:9612674/1‑151 (MQ=255)
                                    tGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCGACAACCAGAAGCtctc                                                                                                                    >  2:6060823/1‑151 (MQ=255)
                                     gTTCGGCAACAATCTCGACCGTCTCAATCCCGACATCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTcc                                                                                                                   <  2:3614176/151‑1 (MQ=255)
                                     gTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTcc                                                                                                                   <  1:84300/151‑1 (MQ=255)
                                         ggCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAAc                                                                                                               >  1:6568827/1‑151 (MQ=255)
                                         ggCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAAc                                                                                                               <  1:6934337/151‑1 (MQ=255)
                                           caacaaTCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTTACGAAGATAGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACgg                                                                                                             <  2:8886702/151‑1 (MQ=255)
                                            aacaaTCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGgc                                                                                                            <  1:8927158/151‑1 (MQ=255)
                                             acaaTCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGgcg                                                                                                           >  2:6568354/1‑151 (MQ=255)
                                             acaaTCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGgcg                                                                                                           >  1:2226838/1‑151 (MQ=255)
                                              caaTCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACCAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGgcgc                                                                                                          >  2:8573482/1‑151 (MQ=255)
                                              caaTCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGgcgc                                                                                                          <  1:9309288/151‑1 (MQ=255)
                                              caaTCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGgcgc                                                                                                          >  2:3144940/1‑151 (MQ=255)
                                               aaTCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGgcgcg                                                                                                         >  1:10193219/1‑151 (MQ=255)
                                                   tcGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGa                                                                                                     >  2:6379625/1‑151 (MQ=255)
                                                    cGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGaa                                                                                                    >  1:9181907/1‑151 (MQ=255)
                                                     gACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAAt                                                                                                   >  2:5829590/1‑151 (MQ=255)
                                                        cGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCgg                                                                                                >  1:611483/1‑151 (MQ=255)
                                                         gTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGt                                                                                               <  2:9100641/151‑1 (MQ=255)
                                                         gTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGt                                                                                               >  2:281250/1‑151 (MQ=255)
                                                           ctcAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAg                                                                                             >  2:5596692/1‑151 (MQ=255)
                                                           ctcAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAAGCGGTAg                                                                                             >  2:10414813/1‑151 (MQ=255)
                                                            tcAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGc                                                                                            >  2:7990524/1‑151 (MQ=255)
                                                            tcAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGc                                                                                            >  1:3998322/1‑151 (MQ=255)
                                                             cAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGcc                                                                                           <  1:8291428/151‑1 (MQ=255)
                                                                 cccGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCgg                                                                                       >  1:4529607/1‑151 (MQ=255)
                                                                   cGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGgtg                                                                                     <  1:581089/151‑1 (MQ=255)
                                                                    gACAGCCGCCACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGgtgt                                                                                    >  2:8267638/1‑151 (MQ=255)
                                                                    gACAGCCGCAACACCCTGACGAAGATCGCCCACGCCGTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGgtgt                                                                                    <  2:4661135/151‑1 (MQ=255)
                                                                     aCAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGtt                                                                                   <  2:1380793/151‑1 (MQ=255)
                                                                     aCAGCCGCAACACCCTGACGAAGATCGACCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCTCGCCGAATCGGTAGCAGCGGTGtt                                                                                   <  2:8643855/151‑1 (MQ=255)
                                                                      cAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTc                                                                                  >  1:1813621/1‑151 (MQ=255)
                                                                       aGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTcc                                                                                 >  1:2496700/1‑151 (MQ=255)
                                                                        gCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTCGCCGCGGTGTTCcg                                                                                >  2:6734870/1‑151 (MQ=255)
                                                                         ccGCACCACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGGTCcgc                                                                               <  1:2073000/151‑1 (MQ=255)
                                                                          cGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCcgcg                                                                              >  2:9633148/1‑151 (MQ=255)
                                                                             aaCACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAg                                                                           >  2:7381449/1‑151 (MQ=255)
                                                                                acCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCg                                                                        <  1:1887937/151‑1 (MQ=255)
                                                                                  ccTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACCACTACGGCGACGAAGGCTACAACCAGCAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGc                                                                      <  2:5349894/151‑1 (MQ=255)
                                                                                    tGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACCCCTGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCAt                                                                    <  1:1283349/151‑1 (MQ=255)
                                                                                        gacgATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAACCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCcg                                                                <  1:7799719/148‑1 (MQ=255)
                                                                                          aGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCcggc                                                              >  1:4806706/1‑151 (MQ=255)
                                                                                               gCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCa                                                         >  1:2957937/1‑151 (MQ=255)
                                                                                                   aCGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACAt                                                     <  2:5121120/151‑1 (MQ=255)
                                                                                                    cGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACCCCGACACCTACGGCGACGACGGCTACAACCAGACGCTCTCCGACCGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATc                                                    <  2:233597/151‑1 (MQ=255)
                                                                                                    cGCCTTGCTCGCGGTAGACAACGACAAGGTCCGCCTTGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATc                                                    <  1:589414/151‑1 (MQ=255)
                                                                                                     gCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCg                                                   >  1:2359120/1‑151 (MQ=255)
                                                                                                     gCCTTCCTCGCGGTCGACATCGACAAGGACCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCg                                                   <  2:7426225/151‑1 (MQ=255)
                                                                                                       cgtGCTCGCGGTAGACATCGACACGGTCCGCCTGGAAGGCCACACCGGCAACTACCGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAg                                                 <  2:1888611/149‑1 (MQ=255)
                                                                                                       cTTGCTCGCGGTCGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGGTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAg                                                 <  2:2682947/151‑1 (MQ=255)
                                                                                                       cTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAg                                                 >  2:2515758/1‑151 (MQ=255)
                                                                                                        ttGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACACCTACGGCGACGAAGGCTACAACCAGACGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAgg                                                <  2:3117250/151‑1 (MQ=255)
                                                                                                           cTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTcc                                             <  2:9337481/151‑1 (MQ=255)
                                                                                                            tCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCcg                                            <  1:3302549/151‑1 (MQ=255)
                                                                                                             cgcgGTAGACATCGACAAGGTCAGCCTGGAAGGACACACCGACAACTCCGGCTACGAAGGCAACAACAAGAACCTCTCCGAACGGCGCGCCGAATCGGTAGACGCGGTGTGACGCGAATCCGGCATGCAGGCTGCCAACATCGAGGTCcgc                                           >  2:7844532/1‑151 (MQ=255)
                                                                                                              gcgGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCcgcg                                          <  2:4959356/151‑1 (MQ=255)
                                                                                                                 gTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCCGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGcc                                       <  1:4659307/151‑1 (MQ=255)
                                                                                                                   aGACATCGACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTg                                     <  1:3182171/151‑1 (MQ=255)
                                                                                                                   aGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCGCTCCGACCGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTg                                     >  2:4207066/1‑151 (MQ=255)
                                                                                                                    gACATCGACAAGGTCCGCCTGGCAGGCCACACCGACACCTACGGCGACGAAGGCTACACCCCGACGCTCTCCGAACGGCGCGCCGATTCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTgg                                    <  2:7588312/151‑1 (MQ=255)
                                                                                                                       aTCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCa                                 >  2:6275898/1‑151 (MQ=255)
                                                                                                                          gACAAGGTCCGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCCGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGGGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGa                              <  2:2114065/151‑1 (MQ=255)
                                                                                                                          gACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGa                              >  2:10728144/1‑151 (MQ=255)
                                                                                                                              aGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCaa                          <  1:7149132/151‑1 (MQ=255)
                                                                                                                                gTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGc                        <  1:10443218/151‑1 (MQ=255)
                                                                                                                                gTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGc                        >  1:9782629/1‑151 (MQ=255)
                                                                                                                                 tCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGcc                       >  2:3379726/1‑151 (MQ=255)
                                                                                                                                   cGCCTGGAAGGCCACACCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGCCgg                     >  2:3934575/1‑151 (MQ=255)
                                                                                                                                      cTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGCCGGTgg                  >  1:4272305/1‑151 (MQ=255)
                                                                                                                                        ggAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGCCGGTGGcc                <  1:2026700/151‑1 (MQ=255)
                                                                                                                                         gAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGCCGGTGGCCg               >  1:3607590/1‑151 (MQ=255)
                                                                                                                                                acacCGGCAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGCCGGTGGCCGATAACaa        >  1:3567893/1‑151 (MQ=255)
                                                                                                                                                    cGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGCCGGTGGCCGATAACAAGAcc    <  1:4158727/151‑1 (MQ=255)
                                                                                                                                                     gACACCTACGGCGACGAAGGCTACAACCAGAAGCACTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGCCGGTGGCCGATAACAAGAccc   <  2:7245872/151‑1 (MQ=255)
                                                                                                                                                     gACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGCCGGTGGCCGATAACAAGAccc   >  2:7028478/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
ACGAAGGCTGGGAGTTCGGCATGTCCAGCAAGGTGCTGTTCGGCAACAATCTCGACCGTCTCAATCCCGACAGCCGCAACACCCTGACGAAGATCGCCCACGCCTTGCTCGCGGTAGACATCGACAAGGTCCGCCTGGAAGGCCACACCGACAACTACGGCGACGAAGGCTACAACCAGAAGCTCTCCGAACGGCGCGCCGAATCGGTAGCCGCGGTGTTCCGCGAAGCCGGCATGCCGGCGGCCAACATCGAGGTCCGCGGCCTGGGCATGAGCAAGCCGGTGGCCGATAACAAGACCCG  >  NC_008463/4436864‑4437164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑