Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,616,4540TG31.9% 237.5 / 29.6 123intergenic (‑174/+28)hda/PA14_RS21180DnaA regulatory inactivator Hda/DUF2066 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (32/49);  new base G (38/0);  total (74/49)
Fisher's exact test for biased strand distribution p-value = 7.02e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 127 total aligned reads displayed.

AACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGCCCAG  >  NC_008463/4616305‑4616604
                                                                                                                                                        |                                                                                                                                                           
aaCGCCGCAGGAGTCCCCGTAACGGCGGCAAAGTTTATCCGCCTCGGCAAGCCCGCACACTGAGCAACTCCGCAAGCAAAATCAAACGTGTGCCGATAGCCGAGGCGCCTTGCCCAGAGCATCGGCCGGG‑CGCGCGCCGGCCCGC‑ACC‑Gtt                                                                                                                                                            <  2:9938934/151‑1 (MQ=255)
aaCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑Gtg                                                                                                                                                            >  2:6602289/1‑150 (MQ=255)
 aCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAACTCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTg                                                                                                                                                           <  2:3901718/151‑1 (MQ=255)
 aCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCACAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTg                                                                                                                                                           <  2:6783808/151‑1 (MQ=255)
 aCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTg                                                                                                                                                           <  1:10512853/151‑1 (MQ=255)
  cgccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGc                                                                                                                                                          <  2:8066253/151‑1 (MQ=255)
   gccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGGGCCGGCCCCC‑CCC‑GGTgga                                                                                                                                                         >  2:4515978/1‑149 (MQ=255)
   gccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCa                                                                                                                                                         <  2:10209037/151‑1 (MQ=255)
     cgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGGGCCGGCCCCC‑CCC‑GGTGCG‑Ta                                                                                                                                                      >  1:4723538/1‑151 (MQ=255)
      gcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAg                                                                                                                                                     <  1:2005886/151‑1 (MQ=255)
      gcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAg                                                                                                                                                     <  1:2885095/151‑1 (MQ=255)
       cAGGAGTCACCGTAAAGGCTGCACAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAgg                                                                                                                                                    <  2:9360968/151‑1 (MQ=255)
        aGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGGCGGCCCGC‑GCC‑GGTggaaatgt                                                                                                                                                    >  2:4407798/1‑144 (MQ=255)
        aGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGa                                                                                                                                                   <  1:2980240/151‑1 (MQ=255)
         ggAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAg                                                                                                                                                  <  1:1712127/151‑1 (MQ=255)
          gAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGc                                                                                                                                                 <  1:7418317/151‑1 (MQ=255)
           aGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCCAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCg                                                                                                                                                <  1:2546586/151‑1 (MQ=255)
           aGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCg                                                                                                                                                <  1:6202540/151‑1 (MQ=255)
           aGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCCGCCCGC‑ACC‑GCTGCA‑TAgggggg                                                                                                                                                >  2:2690311/1‑147 (MQ=255)
            gTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCgg                                                                                                                                               <  2:2701086/151‑1 (MQ=255)
            gTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑CGTGGC‑TAGGAGccg                                                                                                                                               >  2:8573007/1‑149 (MQ=255)
            gTCACCGTAAAGGCAGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCgg                                                                                                                                               <  2:3794524/151‑1 (MQ=255)
             tCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGa                                                                                                                                              <  1:7162535/151‑1 (MQ=255)
               aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATc                                                                                                                                            <  1:720616/151‑1 (MQ=255)
                ccGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCg                                                                                                                                           <  1:8624470/151‑1 (MQ=255)
                 cGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCgg                                                                                                                                          <  1:9568349/151‑1 (MQ=255)
                  gTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCCGCCCCC‑TCC‑GGTGGA‑AAGGGGCGGATCGgc                                                                                                                                         >  1:7159798/1‑151 (MQ=255)
                   tAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGgcg                                                                                                                                        <  2:9208352/151‑1 (MQ=255)
                   tAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCCGCCCGC‑TCC‑CGTGCC‑TAGGAGCGGATCGgcg                                                                                                                                        >  2:7431893/1‑151 (MQ=255)
                   tAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCGTGCCCAGAGCATCGGCCGGG‑CGCGCGCCGGCCCGC‑ACC‑CTTGCC‑GAGGCGCGGATAgggg                                                                                                                                        >  2:6275629/1‑149 (MQ=255)
                    aaaGGCTGCAAAGTTTATCCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGgcgc                                                                                                                                       <  2:6087876/151‑1 (MQ=255)
                    aaaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGgcgc                                                                                                                                       <  2:6087899/151‑1 (MQ=255)
                    aaaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGgcgc                                                                                                                                       <  2:6085048/151‑1 (MQ=255)
                       ggCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGcc                                                                                                                                    <  2:2655407/151‑1 (MQ=255)
                        gCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCg                                                                                                                                   <  1:8444027/151‑1 (MQ=255)
                        gCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCCGCCCGC‑CCC‑CGTGGC‑TAAGAGGGGGTAGGCGCGCCg                                                                                                                                   >  1:10656552/1‑151 (MQ=255)
                        gCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCAGCGGCCGGT‑CGCGCGCCCGCCCGC‑TCC‑CGTGGA‑TAGGGGCGGAGCGGCGccccc                                                                                                                                   >  2:516728/1‑150 (MQ=255)
                        gCTGCAAAGTTTAGACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAACGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCg                                                                                                                                   <  1:9027324/151‑1 (MQ=255)
                         cTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTGGCA‑TAGGAGCGGATAGGCGCGCCgt                                                                                                                                  >  2:8002072/1‑150 (MQ=255)
                           gCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑GCC‑GGTGGA‑AAGGGGCGGCTCGGCGCGCCGCCt                                                                                                                                >  2:10160000/1‑151 (MQ=255)
                            cAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGGGCCGGCCCCC‑GCC‑GGTGCC‑TATGAGCGGCTCGGCGCGCCgccac                                                                                                                               >  2:644131/1‑147 (MQ=255)
                             aaaGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGGGCCCGCCCCC‑ACC‑CTTGCA‑GAGGTGGGGGTAGGCGCGCCgccaaa                                                                                                                              >  1:3430048/1‑146 (MQ=255)
                                  ttATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCCC‑GCC‑GGTGCG‑TATGAGCGGGTGGGCGCGCCGACTCagcgcc                                                                                                                         >  2:734528/1‑146 (MQ=255)
                                   tataCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGGGCCGGCCCCC‑GAC‑CGGGGA‑AATGAGCGGAGAGGCGCGCCGACACACAGccc                                                                                                                        >  1:314521/1‑150 (MQ=255)
                                    ataCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAATGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGGGCCGGGCCGC‑GCC‑GGTGGG‑GAGGGGCGGCTCGGCGCGCCGCCGCACAGCCA‑g                                                                                                                      >  2:5498404/1‑151 (MQ=255)
                                    ataCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑GCC‑GTTGCC‑TAGGGGCGGATCGGCGCGCCGACACACAGcccc                                                                                                                       >  2:8028944/1‑149 (MQ=255)
                                    ataCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑GCC‑GGTGGA‑TGGGGGCGGCTCGGCGCGCCGACTCACAGcccg                                                                                                                       >  2:1627497/1‑149 (MQ=255)
                                     taCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑CGTGCA‑TAGGTGCGGTTCGGCGCGCCGCCTCACTGCCC‑G‑c                                                                                                                    >  2:7728525/1‑151 (MQ=255)
                                     taCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCCGCCCGCACCC‑GAGGCA‑‑AGGTGCGGAGCGGGGCGCCGacacacaaacccc                                                                                                                      >  1:3509423/1‑144 (MQ=255)
                                            cGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGGGCCGGCCCCC‑GCC‑GGGGGA‑CAGGAGCGGGGCGGCGCGCCCGCACACAGCCC‑GCCGGAAAt                                                                                                              >  1:10462496/1‑151 (MQ=255)
                                            cGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCCC‑CCC‑CGTGCA‑GGGGATCGGAGCGGCGCGCCGACACACAGCCC‑G‑ccgcacat                                                                                                             >  1:10462485/1‑144 (MQ=255)
                                                aaGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGGCGGCCCGC‑ACC‑GGTGGA‑TAGGGGGGGATCGGGGCGCCCCCACACAGCCC‑C‑CGGGAAttgaag                                                                                                         >  1:7787839/1‑147 (MQ=255)
                                                   gCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGGCGGCCCCC‑ACC‑GGTGCA‑TAGGGGGGGGGCCGCGCGCCGCCACCCCGGCC‑G‑CGGCAAATG‑AGTTgg                                                                                                     >  2:8338277/1‑151 (MQ=255)
                                                     cGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCCC‑GCC‑GGTGGA‑TAGGAGCGGCTGGGCGCGCCGCCACACAGCCC‑G‑CGGAAAATC‑AGGTGGga                                                                                                   >  2:1641532/1‑151 (MQ=255)
                                                            ttaGCAACTTGGCAACCAAAACCATCTTTTGGCCTATCGCCGCGGCTCGTTGCCCTGCG‑ATGGGGGGGTGCGCGCGCCGGCCCGC‑CCC‑GTGTCT‑TAGGCGGGTAGCGGCGCCCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGgacgacgac                                                                                            <  2:9545359/149‑1 (MQ=255)
                                                             gAGCAACTAGGCAAGCAATATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCCGAGCATCGGCCGGT‑CGCGCGCCCGCCCGG‑GCC‑CTGGCG‑TACGGTCGGGTGGGGGCGCCGAGGCACGGGCC‑G‑CGGGACTTC‑CGGGGGGacgacgtcg                                                                                           >  2:10435575/1‑151 (MQ=255)
                                                                cAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑CCC‑CGTGCA‑TAGGTGCGGGTCGGCGCGGCGGCAAACAGCCA‑C‑CCGCAAATC‑AATTGGGACGACGACCGCa                                                                                        >  1:7868739/1‑151 (MQ=255)
                                                                     aGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCCGCCCCCGCCC‑GTTGCA‑‑AGGAGCGGATCGGGGCGCCCCCACACAGCCC‑C‑CGCCAAATA‑AGGTGGGCCGAACACGGCAccccc                                                                                   >  2:2225172/1‑151 (MQ=255)
                                                                        caagcaaAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGGGCCCGCCCCC‑GCC‑GGTTGA‑AAAGTGCGGATAGGCGCGCCCCCGCACAGCCC‑C‑CGGCAATAG‑AATTGGGTAGACCACGGCACCCCcccg                                                                                >  2:4596254/1‑151 (MQ=255)
                                                                        caagcaaAATCAAAGGGTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGGCGGGCCGC‑GCC‑GTTGCA‑GTGGAGCGGCTAGGCGCGCCGAGGCACATCCA‑G‑CGGCATTTG‑GGGTGGGCCGCCGTCGGCAGCGCcgcg                                                                                >  2:10545304/1‑151 (MQ=255)
                                                                         aagcaaAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑AGC‑CTTTCA‑AAGGAGCGGATACGCGCGGCGACCCAAAGCCA‑G‑CGGAGAATA‑AGGTGGGACGAAGGAGGCAAccccgccc                                                                               >  2:705312/1‑149 (MQ=255)
                                                                             aaaaTCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑GCC‑GGTGGA‑AAGGAGCGGATCGGCGCGCCGCCACACAGCCC‑G‑CGGGCAATC‑AGGTTGGTCGACGAACGCACCCCCCCGCTggg                                                                           >  2:1591941/1‑151 (MQ=255)
                                                                                 tCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCCGCCCGC‑GAC‑CTTGGA‑AAGTATCCGGTCGGGGGCCCGACGCAATGCCACG‑GGGGAATAA‑GGTTGGG‑CGAGCACGGCACCCCCGCGGAGGgcgcg                                                                       >  2:1098444/1‑150 (MQ=255)
                                                                                 acAAGGGTTTTCCTATCGCCGTGGCACCTCCCCAAGTGACTCGGCGGGT‑CGCGCGCCGGCCCGC‑TCG‑GTTGCT‑TTGGTGCGTTCGGGCGCCCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCt                                                                       <  2:8888419/150‑1 (MQ=255)
                                                                                      ggTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑GCC‑CTTGCA‑TAAGGGCGGATCGGCGCGCCCACACACAGCCA‑G‑CGGGAAATA‑AGTTGGGGAGACGCCGGCACCGCCGCGCAGGGGGCTGCcgg                                                                  >  2:2566075/1‑151 (MQ=255)
                                                                                       gTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGGGCCCGCCCGC‑GCC‑GGTGGC‑TAGGTGCGGGTAGGCGCGCCGACACACAGCCA‑C‑CGGGAAATA‑AGGTGGGTCGACGACGGCACCCCCGCGCTGGGCGCTGCGGGc                                                                 >  2:7968676/1‑151 (MQ=255)
                                                                                            ccGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCCC‑GCC‑CAGGGC‑AACGAGCGGGGCCGCGCCCCCCCGCACGTCCC‑G‑CGGGAAGAA‑ACGTGGGTCGGCGACGGCCCCCCCGCGCGGGGCGCGGCGGGCGGGc‑c                                                           >  2:7061682/1‑151 (MQ=255)
                                                                                            ccGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCCGCCCCC‑ACC‑GGTGCA‑TTGGGGCGGGTGGGGGCGGCGCCAACCTGCCC‑C‑CGGCAAATA‑AGGTGGGGCGCCGAAGGCACCCCCCCCCCGGGGGCGGCGGGCGGGc‑c                                                           >  2:10789201/1‑151 (MQ=255)
                                                                                                 aGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCCGGCCCC‑ACC‑GTTGCA‑GAGGAGCGGATCGGCGCGCCGCCTCACAGCCC‑G‑CGGAAAATC‑AGGGGGGACGAAGACGGCACCGCCCCGCTGGGGGCGGCGGGCAGGG‑CGGAgg                                                      >  2:8889046/1‑151 (MQ=255)
                                                                                                   ccGAGGCTCCGTCCCCACACCATGGCCCGGC‑CGCGCCCCGCCCCGC‑ACG‑GTGCCT‑TGGGAGCGGTCCGCCCCCCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCa                                                    <  1:8090394/151‑1 (MQ=255)
                                                                                                    cGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGGGGCCGCCCCC‑ACC‑CGTGGA‑GAGGAGCGGGGAGGCGCCCCGACGCACATCCC‑C‑CGGGCAATA‑AGGTGTGGCGACGACCGCACCCCCCCGCGGGGGGCGGCGGGCGGGG‑CGGAGGcca                                                   >  2:4508175/1‑149 (MQ=255)
                                                                                                     gAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGGGCCGGCCCCC‑GCC‑CGTGGC‑GAGGGGCGGGTAGGCGCGCCGCCACACAGGCC‑C‑CGGACATTC‑CCTTTGGGCGACGACCGCCCCGCCGCGCCGGGCGCTGCGGGCGGGC‑CGgggggcac                                                  >  2:8430757/1‑147 (MQ=255)
                                                                                                     gAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑ACC‑CGTTCC‑TAGGAGCGGCTCGGCGCGCCCACACACTGCCC‑C‑CGGCAATTC‑AGTTGGGGCGAACTCCGCCCCGCCGCGCTGGGCGCGGCGGGCTGGG‑CGGAGGcgac                                                  >  2:5953763/1‑148 (MQ=255)
                                                                                                         cTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGGCCGGCCGC‑ACCGGTTGC‑‑TAGGAGCGGTTCTGGGCGGCGAAAGCAAGCCA‑G‑CGGAAAATCAAGTTGGCGGGA‑GACGGCCCCGCCCCGCCGGGGGCGTCGGGCTGGC‑Cggcggcctacacc                                              >  2:1157649/1‑144 (MQ=255)
                                                                                                                cccAGAGCATCGGCCGGT‑CGCGGGCCCGCCCGCGCCC‑GTTG‑A‑GATGAGCGGATAGGGGCGCCGCCACACACGCA‑G‑CCGGAATTA‑ACATGGGGCGGAGACAGCGACGCCGCGCCGGGGGCTGCGGGGGGGCGCGGTGG‑ATCCAGAGGTACGc                                       >  2:4753989/1‑151 (MQ=255)
                                                                                                                  ctgagCAGGGGCCGGT‑GCGGCGCCGCCCCGC‑GCC‑GTGCCA‑TCGGTGCGTAGGGCCGCCCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTg                                     <  2:8530404/149‑1 (MQ=255)
                                                                                                                     tctaggggCCGTT‑GGCGCGCGGGCCCGC‑TCG‑GTTCCA‑TTGGGGCGGAGGGGCGCCCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCg                                  <  2:713277/145‑1 (MQ=255)
                                                                                                                     tccATCGGCCGGT‑CGCGCGCGGGCCCGC‑‑CC‑GTTGGATTTGGCGCTCCTGGGCGCCCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCg                                  <  2:4919596/149‑1 (MQ=255)
                                                                                                                     agCTTCGGCCGGT‑CGCGCGCGGCCCCGC‑GCC‑TTCCAA‑TTGGAGCGCCTGGGCGCCCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCg                                  <  2:4920602/151‑1 (MQ=255)
                                                                                                                      ccaccGGCCGGG‑CGCGCCCCGCCCCGC‑CCG‑GTTCCA‑TTGGCGCGGCCCGGCGCCCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCgg                                 <  2:8902051/147‑1 (MQ=255)
                                                                                                                          gggCCGCG‑CGCGCGCCGGCCCTC‑TCC‑TTTGCT‑TTGCCGCGGTCCGGCGCCCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCgg                             <  2:2794442/150‑1 (MQ=255)
                                                                                                                             gcggggcgcgcgCCGGCCCGC‑TCG‑GTTCCA‑GCGGCGGGGCTGGCCCCCCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGa                           <  1:9420447/145‑1 (MQ=255)
                                                                                                                                     cgcgcCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAACTTC‑AGTTGGGACGACGTCGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGc                   >  1:6391894/1‑151 (MQ=255)
                                                                                                                                     cgcgcCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGc                   >  2:8627391/1‑151 (MQ=255)
                                                                                                                                     cgcgcCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTACCTGc                   >  2:8628332/1‑151 (MQ=255)
                                                                                                                                      ccccccccccGC‑TCC‑TTTTCA‑TTGTCGCGGACCGGCCCCCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCa                  <  2:4507099/144‑1 (MQ=255)
                                                                                                                                       cgcCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGACATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCaa                 >  1:6218465/1‑151 (MQ=255)
                                                                                                                                       cgcCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCGGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCaa                 >  2:4347429/1‑151 (MQ=255)
                                                                                                                                        gcCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAAc                >  1:4139730/1‑151 (MQ=255)
                                                                                                                                        gcCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAAc                >  2:3411765/1‑151 (MQ=255)
                                                                                                                                         ccgccccgc‑cCC‑GTTGCA‑TCGGCGCGCCTCGGCCCCCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACt               <  2:2972923/151‑1 (MQ=255)
                                                                                                                                          cGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTg              >  1:6265576/1‑151 (MQ=255)
                                                                                                                                          cGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTg              >  1:6268736/1‑151 (MQ=255)
                                                                                                                                          cGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTg              >  2:5591171/1‑151 (MQ=255)
                                                                                                                                            gCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcg            >  1:7360861/1‑151 (MQ=255)
                                                                                                                                               cGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCa         >  2:7900579/1‑151 (MQ=255)
                                                                                                                                                gC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAg        >  2:6797572/1‑151 (MQ=255)
                                                                                                                                                gC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAg        >  1:5079016/1‑151 (MQ=255)
                                                                                                                                                   tCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGc      >  2:7185087/1‑151 (MQ=255)
                                                                                                                                                    cc‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGcc     >  1:8376315/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                           
AACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGT‑CGCGCGCCGGCCCGC‑TCC‑GTTGCA‑TAGGAGCGGATCGGCGCGCCGACTCACTGCCA‑G‑CGGAAATTC‑AGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGC‑CGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGCCCAG  >  NC_008463/4616305‑4616604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑