Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,840,444 T→C intergenic (+44/‑45) PA14_RS13335 → / → PA14_RS13340 LysR substrate‑binding domain‑containing protein/sigma‑70 family RNA polymerase sigma factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,840,4440TC100.0% 1773.8 / NA 459intergenic (+44/‑45)PA14_RS13335/PA14_RS13340LysR substrate‑binding domain‑containing protein/sigma‑70 family RNA polymerase sigma factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (222/237);  total (222/237)

Only 100 of 459 total aligned reads displayed.

CCGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTTTTGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTGG  >  NC_008463/2840297‑2840588
                                                                                                                                                   |                                                                                                                                                
 cGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACa                                                                                                                                              <  2:1414337/151‑1 (MQ=255)
   gCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAAt                                                                                                                                            >  2:4316391/1‑151 (MQ=255)
    cGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATg                                                                                                                                           <  2:2733961/151‑1 (MQ=255)
    cGAGGGCGCGGGGGGTATGTGCTGGCGCGACGAACCGCCTCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATg                                                                                                                                           <  2:2087285/151‑1 (MQ=255)
     gAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGc                                                                                                                                          >  1:6487867/1‑151 (MQ=255)
     gAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGc                                                                                                                                          >  1:5145297/1‑151 (MQ=255)
        ggCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGAt                                                                                                                                       <  1:7781979/151‑1 (MQ=255)
             ggTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCAtt                                                                                                                                  >  2:2575068/1‑151 (MQ=255)
              gTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTa                                                                                                                                 >  2:5499326/1‑151 (MQ=255)
                   tATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGACGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCAGTAtcatt                                                                                                                            <  1:4511549/151‑1 (MQ=255)
                   tATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcatt                                                                                                                            >  2:2486392/1‑151 (MQ=255)
                   tATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcatt                                                                                                                            <  1:8530202/151‑1 (MQ=255)
                      gtgCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattcat                                                                                                                         <  2:3143593/151‑1 (MQ=255)
                       tgCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCAtg                                                                                                                        <  1:617756/151‑1 (MQ=255)
                       tgCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCAtg                                                                                                                        >  2:9009336/1‑151 (MQ=255)
                           ggCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTc                                                                                                                    <  1:188020/151‑1 (MQ=255)
                              gcgACGAACCGCCTCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGcc                                                                                                                 <  2:6478089/151‑1 (MQ=255)
                              gcgACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTCTTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGcc                                                                                                                 >  2:6027768/1‑151 (MQ=255)
                                gacgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGcccc                                                                                                               >  2:5309068/1‑151 (MQ=255)
                                gacgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGcccc                                                                                                               >  1:4931159/1‑151 (MQ=255)
                                   gaACCGCATCGCGACCGCGCCAGCGAAGAGCGGCTGGCCTGCCTGCGCGAAGGCGCGGCGGGCGAGTAGGGCGCCGGCCGGCGGGTGCTTTGGCAATAAGGCCGCGTCTTCTCGACAATGCGATTCATGATCATTCATGTGTCGCCCCGAc                                                                                                            <  2:8669113/151‑1 (MQ=255)
                                   gaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGAc                                                                                                            <  2:7992508/151‑1 (MQ=255)
                                    aaCCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACt                                                                                                           >  2:5243927/1‑151 (MQ=255)
                                    aaCCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACt                                                                                                           <  1:2089310/151‑1 (MQ=255)
                                      ccGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACttt                                                                                                         <  2:3651354/151‑1 (MQ=255)
                                      ccGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCACGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACttt                                                                                                         <  1:2067289/151‑1 (MQ=255)
                                       cGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTCTTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTc                                                                                                        >  2:10311392/1‑151 (MQ=255)
                                         caacgcgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTTCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCAATATCATACATGTGTCGCCCCGACTTTccc                                                                                                      <  2:970090/148‑1 (MQ=255)
                                         cATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTccc                                                                                                      <  1:6260527/151‑1 (MQ=255)
                                         cATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTccc                                                                                                      <  2:9095984/151‑1 (MQ=255)
                                          aTCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGTCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCa                                                                                                     >  2:9232645/1‑151 (MQ=255)
                                          aTCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTCTTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCa                                                                                                     >  2:8544435/1‑151 (MQ=255)
                                             gcgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGt                                                                                                  <  2:5653857/151‑1 (MQ=255)
                                              cgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGCAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCACAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCCTTCATGTGACGCCCCGACTTTCCCATGTc                                                                                                 <  2:10223464/151‑1 (MQ=255)
                                              cgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTc                                                                                                 >  1:7974648/1‑151 (MQ=255)
                                                aCCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGAGTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCg                                                                                               <  1:4238597/151‑1 (MQ=255)
                                                   gcgcCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGccc                                                                                            >  2:9791736/1‑151 (MQ=255)
                                                     gcCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCg                                                                                          >  2:7819076/1‑151 (MQ=255)
                                                       cAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGAGTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGAt                                                                                        >  2:1814169/1‑151 (MQ=255)
                                                           gAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCtt                                                                                    >  1:9901471/1‑151 (MQ=255)
                                                           gAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCtt                                                                                    >  1:10734898/1‑151 (MQ=255)
                                                            aaGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTtc                                                                                   >  1:5874762/1‑151 (MQ=255)
                                                               aCCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGTTCCGTCTCa                                                                                >  1:3157382/1‑151 (MQ=255)
                                                               aCCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCa                                                                                <  1:8978645/151‑1 (MQ=255)
                                                                 ctgctgGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAACAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAgc                                                                              <  1:1581248/151‑1 (MQ=255)
                                                                  tgctgGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCGTCTCAgcg                                                                             >  1:3587629/1‑151 (MQ=255)
                                                                    ctgGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGcc                                                                           <  1:831821/151‑1 (MQ=255)
                                                                     tgGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGAGCTAGTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGccc                                                                          <  2:7385486/151‑1 (MQ=255)
                                                                         ctgcctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCccacca                                                                      >  1:7579153/1‑151 (MQ=255)
                                                                         ctgcctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCccacca                                                                      <  1:3054259/151‑1 (MQ=255)
                                                                            cctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATcg                                                                   <  1:4066040/151‑1 (MQ=255)
                                                                            cctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATcg                                                                   >  1:7013385/1‑151 (MQ=255)
                                                                               gcgcGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCgcgc                                                                <  2:2894507/151‑1 (MQ=255)
                                                                                cgcgAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGcc                                                               >  1:7898385/1‑151 (MQ=255)
                                                                                cgcgAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGcc                                                               >  1:5459102/1‑151 (MQ=255)
                                                                                 gcgAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCca                                                              <  1:10306930/151‑1 (MQ=255)
                                                                                   gAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcatg                                                            >  1:10108894/1‑151 (MQ=255)
                                                                                   gAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcatg                                                            >  1:3898731/1‑151 (MQ=255)
                                                                                        cgcgGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCatgcatgc                                                       <  2:6666352/151‑1 (MQ=255)
                                                                                         gcggcgGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATgcg                                                      <  2:8039473/151‑1 (MQ=255)
                                                                                         gcggcgGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATgcg                                                      <  1:5711551/151‑1 (MQ=255)
                                                                                           ggcggcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCt                                                    <  2:3902755/151‑1 (MQ=255)
                                                                                             cggcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGt                                                  <  2:6764888/151‑1 (MQ=255)
                                                                                              ggcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTa                                                 <  1:95060/151‑1 (MQ=255)
                                                                                              ggcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTa                                                 <  1:82262/151‑1 (MQ=255)
                                                                                                 cGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAg                                              <  1:9997713/151‑1 (MQ=255)
                                                                                                  gAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGc                                             >  1:598110/1‑151 (MQ=255)
                                                                                                  gAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGc                                             <  2:48087/151‑1 (MQ=255)
                                                                                                      aGGGCGCCGGCCGGCGGGTGCTATTGCAATAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCAGGTCCGCCCCGATCCTTCTCAGCGCCCACCCTCGCGCCATGCATGCGCTGTACAGCGAGc                                         <  2:3347058/151‑1 (MQ=255)
                                                                                                      aGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGc                                         >  1:9160179/1‑151 (MQ=255)
                                                                                                         gcgcCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCAcc                                      <  2:10598103/151‑1 (MQ=255)
                                                                                                           gccggccgGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCAt                                    >  1:10470531/1‑151 (MQ=255)
                                                                                                            ccggccggCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATg                                   >  1:9657597/1‑151 (MQ=255)
                                                                                                               gccggcGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCt                                <  1:2839301/151‑1 (MQ=255)
                                                                                                                ccggcGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTg                               >  1:2152355/1‑151 (MQ=255)
                                                                                                                 cggcggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTgg                              >  1:689747/1‑151 (MQ=255)
                                                                                                                 cggcggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTgg                              <  1:8132498/151‑1 (MQ=255)
                                                                                                                    cggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTg                           >  1:5367969/1‑151 (MQ=255)
                                                                                                                      ggTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCa                         >  2:5776381/1‑151 (MQ=255)
                                                                                                                        tGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGa                       >  2:7188682/1‑151 (MQ=255)
                                                                                                                         gCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGCTTCAGTATCATTCAGGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGaa                      <  2:6958443/151‑1 (MQ=255)
                                                                                                                          cTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAAc                     >  1:10011429/1‑151 (MQ=255)
                                                                                                                          cTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAAc                     >  2:1925768/1‑151 (MQ=255)
                                                                                                                           tATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACt                    >  2:5149496/1‑151 (MQ=255)
                                                                                                                           tATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACt                    >  1:3312978/1‑151 (MQ=255)
                                                                                                                            aTTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTg                   >  1:870524/1‑151 (MQ=255)
                                                                                                                             ttGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTgg                  >  1:7097014/1‑151 (MQ=255)
                                                                                                                               gCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGtt                <  2:732773/151‑1 (MQ=255)
                                                                                                                                cAAACAGGCCGCGGCTTCACGACACTGCGATTCAATATCATTCATGTGTCGCCCCGACGTTCCCCTGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTg               <  2:10732083/151‑1 (MQ=255)
                                                                                                                                cAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTg               >  1:2636549/1‑151 (MQ=255)
                                                                                                                                cAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTg               >  2:11393/1‑151 (MQ=255)
                                                                                                                                cAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTg               >  2:3498512/1‑151 (MQ=255)
                                                                                                                                     aGGCCGCGGCTTCTCGACAATGCGATTCATGATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGACCTGGTTGCGCgg          >  1:6039237/1‑151 (MQ=255)
                                                                                                                                      ggCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGc         >  2:7148069/1‑151 (MQ=255)
                                                                                                                                      ggCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGc         <  1:8451890/151‑1 (MQ=255)
                                                                                                                                        ccGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCaa       >  1:7243412/1‑151 (MQ=255)
                                                                                                                                           cgGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCt    >  1:1399954/1‑151 (MQ=255)
                                                                                                                                           cgGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCt    >  2:991760/1‑151 (MQ=255)
                                                                                                                                            ggCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTg   >  1:4699904/1‑151 (MQ=255)
                                                                                                                                            ggCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTg   >  1:1736983/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTTTTGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTGG  >  NC_008463/2840297‑2840588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑