Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,056,947 C→T A404T (GCC→ACC)  PA14_RS14010 ← mannitol dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,056,9470CT99.8% 1884.4 / NA 446A404T (GCC→ACC) PA14_RS14010mannitol dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (212/232);  minor base A (1/0);  total (213/233)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 446 total aligned reads displayed.

GTCTCCACGCCGAGCAGGCGCCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGCCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAGCGCTCGACCAGGCTGTCCTT  >  NC_008463/3056799‑3057094
                                                                                                                                                    |                                                                                                                                                   
  ctcCACGCCGAGCAGGCGCCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCAcc                                                                                                                                                 >  1:10258583/1‑151 (MQ=255)
   tcCACGCCGAGCAGGCGCCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCAccc                                                                                                                                                <  2:7153002/151‑1 (MQ=255)
      aCGCCGAGCAGGCGCCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGtt                                                                                                                                             <  1:2762366/151‑1 (MQ=255)
         ccGAGCAGGCGCCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCa                                                                                                                                          <  2:346783/151‑1 (MQ=255)
         ccGAGCAGGCGCCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCa                                                                                                                                          <  1:6556402/151‑1 (MQ=255)
         ccGAGCAGGCGCCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCa                                                                                                                                          >  2:6878347/1‑151 (MQ=255)
         ccGAGCAGGCGCCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCa                                                                                                                                          <  2:8514243/151‑1 (MQ=255)
          cGAGCAGGCGCCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAg                                                                                                                                         <  2:2516073/151‑1 (MQ=255)
          cGAGCAGGCGCCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAg                                                                                                                                         >  2:7906477/1‑151 (MQ=255)
            aGCAGGCGCCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTg                                                                                                                                       <  1:9668861/151‑1 (MQ=255)
                   gcCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGcggcc                                                                                                                                <  2:5211400/151‑1 (MQ=255)
                   gcCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGcggcc                                                                                                                                >  1:445717/1‑151 (MQ=255)
                    ccGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGcggccg                                                                                                                               >  1:8922948/1‑151 (MQ=255)
                      ggCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGcggccggc                                                                                                                             >  2:10638873/1‑151 (MQ=255)
                        cccAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCcggcgg                                                                                                                           <  1:7743477/151‑1 (MQ=255)
                        cccAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCcggcgg                                                                                                                           <  1:3451803/151‑1 (MQ=255)
                             gCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATc                                                                                                                      >  1:2071951/1‑151 (MQ=255)
                              ccGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGAGCa                                                                                                                     >  2:1509899/1‑151 (MQ=255)
                               cGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAg                                                                                                                    >  1:4570541/1‑151 (MQ=255)
                               cGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAg                                                                                                                    <  2:6659808/151‑1 (MQ=255)
                               cGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAg                                                                                                                    >  1:10353242/1‑151 (MQ=255)
                                 tGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGcc                                                                                                                  <  2:358754/151‑1 (MQ=255)
                                           cAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGt                                                                                                        >  2:4305265/1‑151 (MQ=255)
                                           cAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGt                                                                                                        <  2:5257166/151‑1 (MQ=255)
                                           cAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGt                                                                                                        <  1:2916670/151‑1 (MQ=255)
                                            aaCCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTg                                                                                                       <  2:9051442/151‑1 (MQ=255)
                                                aGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGa                                                                                                   >  2:7282366/1‑151 (MQ=255)
                                                 gcgcCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGAc                                                                                                  <  1:7150378/151‑1 (MQ=255)
                                                   gcCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGa                                                                                                <  2:7840630/151‑1 (MQ=255)
                                                          aTTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACtt                                                                                         <  2:1916110/151‑1 (MQ=255)
                                                          aTTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACtt                                                                                         <  1:3019048/151‑1 (MQ=255)
                                                          aTTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACtt                                                                                         >  2:6827877/1‑151 (MQ=255)
                                                            tCGGCGGCGCGCGGATCGGGAATCGGGTTGTGCGCGCCATGCTCGTCGACCCCGCCCAGGTACCGCGCCCTGGCCGCCACCACCTGGGTCACCGGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTgg                                                                                       <  2:3743047/151‑1 (MQ=255)
                                                             cggcggCGCGCGGATCGGGTATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTggg                                                                                      >  2:965941/1‑151 (MQ=255)
                                                                   cgcgcgGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGCCGATGAACTTGGGAAACtt                                                                                >  2:5475896/1‑151 (MQ=255)
                                                                   cgcgcgGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACtt                                                                                <  2:9254208/151‑1 (MQ=255)
                                                                    gcgcgGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGCCCATGAACTTGGCaaaatac                                                                               >  2:2213803/1‑147 (MQ=255)
                                                                    gcgcgGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTc                                                                               <  2:4818006/151‑1 (MQ=255)
                                                                        ggATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTcgacg                                                                           <  1:8477543/151‑1 (MQ=255)
                                                                        ggATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTcgacg                                                                           <  2:5432329/151‑1 (MQ=255)
                                                                         gATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTcgacga                                                                          >  1:6880333/1‑151 (MQ=255)
                                                                         gATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTcgacga                                                                          <  1:5155456/151‑1 (MQ=255)
                                                                          aTCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAg                                                                         >  1:677478/1‑151 (MQ=255)
                                                                           tCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGc                                                                        <  1:6176869/151‑1 (MQ=255)
                                                                             gggAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTTGGCGGCCGGCGTCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCg                                                                      <  2:9589945/151‑1 (MQ=255)
                                                                             gggAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCg                                                                      <  2:1407075/151‑1 (MQ=255)
                                                                              ggAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGACCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGt                                                                     >  2:10280230/1‑151 (MQ=255)
                                                                                aaTCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCTGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTcc                                                                   <  1:7414207/151‑1 (MQ=255)
                                                                                  tCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGa                                                                 <  1:8037174/151‑1 (MQ=255)
                                                                                   cGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGTGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAg                                                                <  2:2790055/151‑1 (MQ=255)
                                                                                     ggTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCa                                                              >  1:8431580/1‑151 (MQ=255)
                                                                                     ggTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCa                                                              <  1:10039886/151‑1 (MQ=255)
                                                                                     ggTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCa                                                              <  1:6274038/151‑1 (MQ=255)
                                                                                      gTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAg                                                             >  2:9765893/1‑151 (MQ=255)
                                                                                      gTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAg                                                             <  1:9127374/151‑1 (MQ=255)
                                                                                          tGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGAccc                                                         <  1:9862101/151‑1 (MQ=255)
                                                                                           gCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCg                                                        >  1:8183/1‑151 (MQ=255)
                                                                                            cTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGt                                                       <  2:10032979/151‑1 (MQ=255)
                                                                                                ccATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCa                                                   >  2:4103792/1‑151 (MQ=255)
                                                                                                ccATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCa                                                   <  2:3786648/151‑1 (MQ=255)
                                                                                                    gCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTg                                               >  1:5302442/1‑151 (MQ=255)
                                                                                                    gCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTg                                               >  1:10831436/1‑151 (MQ=255)
                                                                                                     cTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTgg                                              <  2:5698450/151‑1 (MQ=255)
                                                                                                     cTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTgg                                              >  1:9255131/1‑151 (MQ=255)
                                                                                                      tcgtcgACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGt                                             <  1:3958285/151‑1 (MQ=255)
                                                                                                       cgtcgACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTc                                            >  2:6337276/1‑151 (MQ=255)
                                                                                                       cgtcgACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGGCACCCGTTCCAGTGGGCGGCCGGCGGCGATCACCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTc                                            <  2:7358223/151‑1 (MQ=255)
                                                                                                        gtcgACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCg                                           <  2:10787920/151‑1 (MQ=255)
                                                                                                         tcgACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCgg                                          >  1:5956450/1‑151 (MQ=255)
                                                                                                         tcgACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCgg                                          >  2:6601065/1‑151 (MQ=255)
                                                                                                          cgACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGc                                         >  2:8285232/1‑151 (MQ=255)
                                                                                                           gACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCg                                        <  2:9481519/151‑1 (MQ=255)
                                                                                                           gACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCg                                        >  1:6354233/1‑151 (MQ=255)
                                                                                                           gACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCg                                        <  1:7510153/151‑1 (MQ=255)
                                                                                                            aCCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGa                                       >  1:1484004/1‑151 (MQ=255)
                                                                                                             ccccGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGAt                                      <  1:10663480/151‑1 (MQ=255)
                                                                                                               ccGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATgg                                    >  1:4802751/1‑151 (MQ=255)
                                                                                                                      ggTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGtt                             >  1:3225604/1‑151 (MQ=255)
                                                                                                                       gTACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTg                            <  2:3309476/151‑1 (MQ=255)
                                                                                                                        tACAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGAGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTAGGTCGGCGATGGCGCGGTTgg                           >  2:5868687/1‑151 (MQ=255)
                                                                                                                          cAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCg                         >  1:6665224/1‑151 (MQ=255)
                                                                                                                          cAGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGAGTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCg                         >  1:1255118/1‑151 (MQ=255)
                                                                                                                           aGCGCCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGa                        <  2:494539/151‑1 (MQ=255)
                                                                                                                            gcgcCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGaa                       >  2:611468/1‑151 (MQ=255)
                                                                                                                            gcgcCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGaa                       <  2:452676/151‑1 (MQ=255)
                                                                                                                             cgcCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAg                      >  2:6011862/1‑151 (MQ=255)
                                                                                                                             cgcCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAg                      >  1:4933621/1‑151 (MQ=255)
                                                                                                                              gcCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAgc                     <  1:1585929/151‑1 (MQ=255)
                                                                                                                              gcCCAGGCCGCCACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAgc                     >  1:10747548/1‑151 (MQ=255)
                                                                                                                                    gccgccACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCCGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAGCGCTCGa               >  1:3629235/1‑151 (MQ=255)
                                                                                                                                    gccgccACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAGCGCTCGa               >  1:4276516/1‑151 (MQ=255)
                                                                                                                                       gccACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAGCGCTCGACCa            >  1:7855218/1‑151 (MQ=255)
                                                                                                                                       gccACCACCAGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAGCGCTCGACCa            <  2:3014252/151‑1 (MQ=255)
                                                                                                                                        ccaccaccaGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAGCGCTCGACCAg           >  2:2105929/1‑151 (MQ=255)
                                                                                                                                         caccaccaGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAGCGCTCGACCAgg          >  1:8592761/1‑151 (MQ=255)
                                                                                                                                            caccaGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAGCGCTCGACCAGGCTg       <  1:7080215/151‑1 (MQ=255)
                                                                                                                                              ccaGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAGCGCTCGACCAGGCTGTc     <  1:5857469/151‑1 (MQ=255)
                                                                                                                                              ccaGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAGCGCTCGACCAGGCTGTc     >  2:3940032/1‑151 (MQ=255)
                                                                                                                                                aGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAGCGCTCGACCAGGCTGTCCt   >  1:5474459/1‑151 (MQ=255)
                                                                                                                                                aGGGTCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGAGCGGTTGGCGAAGCGCTCGACCAGGCTGTCCt   >  1:236448/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTCTCCACGCCGAGCAGGCGCCGGCCCAGGCCGTGGCGTTCGGCAACCAGCGCCTGGCATTCGGCGGCGCGCGGATCGGGAATCGGGTAGTGCTCGCCATGCTCGTCGACCCCGCCCAGGTACAGCGCCCAGGCCGCCACCACCAGGGCCACCCGTTCCAGTGGGCGGCCGGCGGCGATCAGCCGATTGGCGGTGGGGACGATGAACTTGGGAAACTTCGACGAGCCGTCCGAGCAGACCCGTTCCAGTTGGTCGGCGATGGCGCGGTTGGCGAAGCGCTCGACCAGGCTGTCCTT  >  NC_008463/3056799‑3057094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑