Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,791,938 A→G intergenic (+32/‑103) nadE → / → choE ammonia‑dependent NAD(+) synthetase/cholinesterase ChoE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,791,9380AG100.0% 2182.6 / NA 555intergenic (+32/‑103)nadE/choEammonia‑dependent NAD(+) synthetase/cholinesterase ChoE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (264/291);  total (264/291)

Only 100 of 558 total aligned reads displayed.

TATGCCGAGATCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCAACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTCAGCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAAAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCG  >  NC_008463/5791790‑5792086
                                                                                                                                                    |                                                                                                                                                    
 aTGCCGAGATCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACg                                                                                                                                                   <  2:8884961/151‑1 (MQ=255)
  tGCCGAGATCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACgg                                                                                                                                                  >  1:1988842/1‑151 (MQ=255)
  agCCGAGATCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGCTGATCGTCGACACCAACCCCCACACCCCGCCCACGCGCGAACTGCCGACGGCGCCCTGAGCCTCCCGGCCCGAGCGGCAGGCCCGTCGTCGACgg                                                                                                                                                  <  1:1874575/150‑1 (MQ=255)
       agaTCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGCCCCAGCACAAGCGCGTCCAGCCGCAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGGCGTCGACGGGCCtg                                                                                                                                             >  2:7172248/1‑150 (MQ=255)
        gaTCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCTGGCCCGTCGTCGACGGGCCttt                                                                                                                                            >  1:2289779/1‑151 (MQ=255)
        gaTCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCttt                                                                                                                                            <  1:4445498/151‑1 (MQ=255)
            gACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGCCACCTACCACAAGACCCAGCCCCAGCGCGCACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTcg                                                                                                                                        <  1:5823368/151‑1 (MQ=255)
             aCGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTcgc                                                                                                                                       >  1:1441118/1‑151 (MQ=255)
             aCGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTcgc                                                                                                                                       <  2:7729425/151‑1 (MQ=255)
               gCCTTCCTGCACGGCCAGCCGCTGCGCGAGGACGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCgcgg                                                                                                                                     >  1:6356515/1‑151 (MQ=255)
                ccTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCgcggg                                                                                                                                    >  1:2761663/1‑151 (MQ=255)
                ccTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGAGCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCgcggg                                                                                                                                    >  2:1976792/1‑151 (MQ=255)
                 cTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCCCACGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCgcgggc                                                                                                                                   <  2:9552833/151‑1 (MQ=255)
                 cTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCgcgggc                                                                                                                                   <  2:10091498/151‑1 (MQ=255)
                 cTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCgcgggc                                                                                                                                   >  2:8433449/1‑151 (MQ=255)
                   tCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCgcgggcgg                                                                                                                                 <  2:5075873/151‑1 (MQ=255)
                    ccTGCACGGCCAGCCGCTGCGCGGGTAGGCCTCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAGCTGCCGAAGGCGCCGTGAGCCTACCGGCACGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGcgggcggg                                                                                                                                >  2:2596921/1‑151 (MQ=255)
                    ccTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTCCCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGcgggcggg                                                                                                                                >  1:7734513/1‑151 (MQ=255)
                      tGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGaa                                                                                                                              >  2:3126640/1‑151 (MQ=255)
                       gCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTGTGTCGcgggcgggcct                                                                                                                             >  2:1459192/1‑148 (MQ=255)
                            gCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGcc                                                                                                                        <  1:9972250/151‑1 (MQ=255)
                            gCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGcc                                                                                                                        <  2:5377111/151‑1 (MQ=255)
                             ccAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACACGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCt                                                                                                                       <  1:7333832/151‑1 (MQ=255)
                             ccAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACACGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCt                                                                                                                       <  1:832973/151‑1 (MQ=255)
                              cAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTg                                                                                                                      >  2:9942499/1‑151 (MQ=255)
                              cAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTg                                                                                                                      <  1:10545119/151‑1 (MQ=255)
                               aGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGt                                                                                                                     >  1:6809483/1‑151 (MQ=255)
                                 ccGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTcg                                                                                                                   <  2:10564598/151‑2 (MQ=255)
                                      gcgcGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAAcc                                                                                                             <  2:3004673/151‑1 (MQ=255)
                                      gcgcGAGGAAGCCGCGCGGGAGATCGTCGACACCTACCACCAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAAcc                                                                                                             <  2:2209395/151‑1 (MQ=255)
                                        gcgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAg                                                                                                           <  1:3843652/151‑1 (MQ=255)
                                         cgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGTCGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAgg                                                                                                          >  1:4244377/1‑151 (MQ=255)
                                         cgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAgg                                                                                                          >  2:3409511/1‑151 (MQ=255)
                                            ggAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCt                                                                                                       >  1:8152480/1‑151 (MQ=255)
                                             gAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCtt                                                                                                      <  2:5206905/151‑1 (MQ=255)
                                              aaGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGACTGTGCCTGTC‑GCAACCAGGTCTTc                                                                                                     >  2:155669/1‑151 (MQ=255)
                                              aaGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTc                                                                                                     <  1:6279877/151‑1 (MQ=255)
                                              aaGCCGCGCGGGTGATCGTCGACACCGACCACAAGACCCAGCCCAAGCGCGACCTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTc                                                                                                     <  2:6740469/151‑1 (MQ=255)
                                               aGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCa                                                                                                    >  1:5346106/1‑151 (MQ=255)
                                                  cgcgcgGGTGATCGTCGACACCTACCACCAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGt                                                                                                 <  2:7884943/151‑1 (MQ=255)
                                                  cgcgcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGt                                                                                                 >  1:2155650/1‑151 (MQ=255)
                                                  cgcgcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGt                                                                                                 >  2:9612780/1‑151 (MQ=255)
                                                    cgcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTcc                                                                                               >  1:7182500/1‑151 (MQ=255)
                                                      cgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgc                                                                                             >  2:9150847/1‑151 (MQ=255)
                                                       gggTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcg                                                                                            <  1:7630710/151‑1 (MQ=255)
                                                       gggTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcg                                                                                            >  1:937023/1‑151 (MQ=255)
                                                       gggTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGGCGACGGGCCGTTGTCGCGGGCGGGACTGTGCCTGTC‑GCCACCAGGTCTTCCAGTCcgcg                                                                                            >  2:6321372/1‑151 (MQ=255)
                                                        ggTGATCGTCGACACCTACCACACGACCCAGCACAAGCGCGAACTGCCGACGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcgc                                                                                           <  2:10214522/151‑1 (MQ=255)
                                                        ggTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcgc                                                                                           >  1:490240/1‑151 (MQ=255)
                                                         gTGATCGTCGACACCTCCCCCAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcgcg                                                                                          <  2:2458918/151‑1 (MQ=255)
                                                         gTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcgcg                                                                                          >  2:6967154/1‑151 (MQ=255)
                                                              cgtcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcggc                                                                                     <  1:9616557/151‑1 (MQ=255)
                                                              cgtcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcggc                                                                                     >  1:6492107/1‑151 (MQ=255)
                                                               gtcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcggcg                                                                                    >  1:3331430/1‑151 (MQ=255)
                                                                tcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGgcgc                                                                                   <  2:4499428/151‑1 (MQ=255)
                                                                 cgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGgcgcg                                                                                  <  1:6037238/151‑1 (MQ=255)
                                                                  gACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTTCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGgcgcgc                                                                                 >  1:8636465/1‑151 (MQ=255)
                                                                  gACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGgcgcgc                                                                                 >  1:8917357/1‑151 (MQ=255)
                                                                   acacCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCt                                                                                <  1:10048632/151‑1 (MQ=255)
                                                                    cacCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTa                                                                               <  1:2500746/151‑1 (MQ=255)
                                                                     acCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTag                                                                              >  2:27390/1‑150 (MQ=255)
                                                                         aCCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAgg                                                                          >  1:3673167/1‑151 (MQ=255)
                                                                              aaGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCt                                                                     >  2:9678455/1‑151 (MQ=255)
                                                                                  cccAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCGTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAAt                                                                 >  2:8224214/1‑151 (MQ=255)
                                                                                     aGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATccc                                                              >  2:4566329/1‑151 (MQ=255)
                                                                                     aGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATccc                                                              >  2:390383/1‑151 (MQ=255)
                                                                                      gCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCt                                                             <  1:10303765/151‑1 (MQ=255)
                                                                                       cacaAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCTGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTg                                                            <  2:7394858/151‑1 (MQ=255)
                                                                                         caAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCg                                                          <  2:1777782/151‑1 (MQ=255)
                                                                                           aGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGaa                                                        >  1:9319569/1‑151 (MQ=255)
                                                                                            gcgcGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAg                                                       >  2:10780448/1‑151 (MQ=255)
                                                                                                gAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgcca                                                   >  1:5202782/1‑151 (MQ=255)
                                                                                                gAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgcca                                                   <  2:10322131/151‑1 (MQ=255)
                                                                                                  aCTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccagc                                                 <  2:5882576/151‑1 (MQ=255)
                                                                                                   cTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccagcc                                                >  1:7541773/1‑151 (MQ=255)
                                                                                                    tGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccagcca                                               <  2:3964813/151‑1 (MQ=255)
                                                                                                        gAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgcg                                           >  1:6705178/1‑151 (MQ=255)
                                                                                                        gAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGGGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgcg                                           >  1:705151/1‑151 (MQ=255)
                                                                                                          aGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCa                                         <  1:9042526/151‑1 (MQ=255)
                                                                                                           ggCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCAc                                        <  1:118752/151‑1 (MQ=255)
                                                                                                             cgcCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCCTGCGCAccc                                      >  1:3135815/1‑151 (MQ=255)
                                                                                                               cccTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGt                                    <  2:563565/151‑1 (MQ=255)
                                                                                                               cccTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGt                                    <  1:4652225/151‑1 (MQ=255)
                                                                                                               cccTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGt                                    <  1:1627780/151‑1 (MQ=255)
                                                                                                                ccTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtc                                   <  1:5435874/151‑1 (MQ=255)
                                                                                                                  tGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGACGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtctc                                 >  2:6564718/1‑151 (MQ=255)
                                                                                                                  tGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtctc                                 >  1:7502188/1‑151 (MQ=255)
                                                                                                                   gAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCAGGCGCACCCGTCTcc                                >  1:694828/1‑151 (MQ=255)
                                                                                                                       cTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCGTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGc                            >  1:2884459/1‑151 (MQ=255)
                                                                                                                         cccGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGccc                          <  1:6599478/151‑1 (MQ=255)
                                                                                                                              cccGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctc                     >  2:10417474/1‑151 (MQ=255)
                                                                                                                               ccGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcc                    <  2:6099822/151‑1 (MQ=255)
                                                                                                                                 gTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcctc                  <  2:5476419/151‑1 (MQ=255)
                                                                                                                                       cAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTc            >  2:3546770/1‑151 (MQ=255)
                                                                                                                                       cAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTc            >  2:2852565/1‑151 (MQ=255)
                                                                                                                                        aGGCCCGTCGTCGACGGTCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGCTCTTCATGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTcc           <  2:4162564/151‑1 (MQ=255)
                                                                                                                                            ccGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctgct       <  1:4013920/151‑1 (MQ=255)
                                                                                                                                            ccGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctgct       <  1:80427/151‑1 (MQ=255)
                                                                                                                                             cgtcgtCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctgctg      >  1:262789/1‑151 (MQ=255)
                                                                                                                                             cgtcgtCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctgctg      >  2:8658750/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TATGCCGAGATCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCAACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTCAGCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAAAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCG  >  NC_008463/5791790‑5792086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑