Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,809,365 T→C K239E (AAG→GAG)  rlmB ← 23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,809,3650TC100.0% 1524.4 / NA 415K239E (AAG→GAG) rlmB23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (221/194);  total (221/194)

Only 100 of 417 total aligned reads displayed.

CAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCGT  >  NC_008463/5809219‑5809512
                                                                                                                                                  |                                                                                                                                                   
cggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGACGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACa                                                                                                                                                >  2:2884692‑M2/81‑151 (MQ=255)
   caaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGc                                                                                                                                             <  1:6207028‑M2/74‑1 (MQ=255)
   caaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatattggtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGc                                                                                                                                             <  2:10805099‑M2/74‑1 (MQ=255)
    aaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCa                                                                                                                                            >  2:9098071‑M2/77‑151 (MQ=255)
    aaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCACACACCAGCGGGGTGCGCTGGCGGAGGATATCGAACAGGCa                                                                                                                                            >  1:5292558‑M2/77‑151 (MQ=255)
       gggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGAc                                                                                                                                         <  2:5957479‑M2/78‑1 (MQ=255)
         gcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGc                                                                                                                                       >  1:1828457‑M2/72‑151 (MQ=255)
           gacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCg                                                                                                                                     >  2:10717284‑M2/70‑151 (MQ=255)
           gacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCg                                                                                                                                     <  2:8577609‑M2/82‑1 (MQ=255)
            acattctagagcagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCgg                                                                                                                                    <  2:9265141‑M2/83‑1 (MQ=255)
                     agaagggcaaccggcgaagcaaggaagttgggcgaatttttgtacagcgtatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCg                                                                                                                           <  1:10556996‑M2/92‑1 (MQ=255)
                     agaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCCGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCg                                                                                                                           >  1:1690203‑M2/60‑151 (MQ=255)
                       aagggcaaccggcgaagcaaggaagattggcgaatatttgtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCgag                                                                                                                         <  2:9163699‑M2/94‑1 (MQ=255)
                        agggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCgaga                                                                                                                        <  2:8101708‑M2/95‑1 (MQ=255)
                            caaccggcgaagcaaggaagattggcgaatctttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGtt                                                                                                                    <  2:8231303‑M2/99‑1 (MQ=255)
                             aaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGGTc                                                                                                                   >  2:5507121‑M2/52‑151 (MQ=255)
                              accggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCCCGCGCTCACTCCAGCGGGGTGCGCTGGCGGTGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCa                                                                                                                  >  1:7287487‑M2/51‑151 (MQ=255)
                               ccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAg                                                                                                                 <  1:2777701‑M2/102‑1 (MQ=255)
                               ccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAg                                                                                                                 >  1:4279527‑M2/50‑151 (MQ=255)
                                cggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAgg                                                                                                                >  2:2539547‑M2/49‑151 (MQ=255)
                                 ggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgc                                                                                                               >  2:8398500‑M2/48‑151 (MQ=255)
                                  gcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgct                                                                                                              <  1:2686110‑M2/105‑1 (MQ=255)
                                   cgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctg                                                                                                             >  1:1411513‑M2/46‑151 (MQ=255)
                                   cgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctg                                                                                                             >  1:293547‑M2/46‑151 (MQ=255)
                                     aagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctgct                                                                                                           >  1:9750491‑M2/44‑151 (MQ=255)
                                     aagcaaggaagattggcgaatatttgtacagcggagggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGCGCTCGTACAGGCAGACGCCGGTGGCGACCGCGCCGTTCAGgctgct                                                                                                           >  2:7554992‑M2/44‑151 (MQ=255)
                                      agcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCCGCGGGGTGCGCGGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTGaggctgcgg                                                                                                          >  1:3998238‑M2/43‑149 (MQ=255)
                                         aaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACg                                                                                                       <  2:1226140‑M2/112‑1 (MQ=255)
                                         aaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTAACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGTCg                                                                                                       >  2:4356033‑M2/40‑151 (MQ=255)
                                           ggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAGCGAGACGTTCAGGCTGCTGACGCt                                                                                                     >  2:3528076‑M2/38‑151 (MQ=255)
                                           ggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCt                                                                                                     <  2:10272049‑M2/114‑1 (MQ=255)
                                                attggcgaatatttgtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGCCCGAGACGTTCAGGCTGCTGACGCTGCCgg                                                                                                <  2:9904032‑M2/119‑1 (MQ=255)
                                                 ttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGc                                                                                               >  1:7193110‑M2/32‑151 (MQ=255)
                                                   ggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCa                                                                                             >  2:6751185‑M2/30‑151 (MQ=255)
                                                    gcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAt                                                                                            <  1:3759311‑M2/123‑1 (MQ=255)
                                                     cgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATc                                                                                           >  2:1119276‑M2/28‑151 (MQ=255)
                                                      gaatatttgtacagcggatggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCg                                                                                          <  1:2332987‑M2/125‑1 (MQ=255)
                                                       aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCgg                                                                                         >  2:10576700‑M2/26‑151 (MQ=255)
                                                        atatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGc                                                                                        <  1:9572715‑M2/127‑1 (MQ=255)
                                                          atttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCaa                                                                                      <  2:8674760‑M2/129‑1 (MQ=255)
                                                           tttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAAc                                                                                     >  2:10397248‑M2/22‑151 (MQ=255)
                                                            ttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCATCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAccc                                                                                    >  2:4391963‑M2/21‑151 (MQ=255)
                                                               tacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTgg                                                                                 <  1:4377193‑M2/134‑1 (MQ=255)
                                                                  agcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGCTCTCGAACAGGCCGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCAGCCGGCCATCGGCCACCTGGCGa                                                                              >  2:4241301‑M2/15‑151 (MQ=255)
                                                                   gcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCGGGCGGAGGATCTCGAACCGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAg                                                                             >  2:638719‑M2/14‑151 (MQ=255)
                                                                       atggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCAGGCGGAGGATATCGAACAGGCAGAAGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAg                                                                         >  1:5257911‑M2/10‑151 (MQ=255)
                                                                        tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGAAGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGt                                                                        >  2:4106505‑M2/9‑151 (MQ=255)
                                                                        tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGAACTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCAGGCGAGGAAGt                                                                        >  2:3083026‑M2/9‑151 (MQ=255)
                                                                        tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCAAGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCGGGCGAGGTagg                                                                        >  1:5644599‑M2/9‑150 (MQ=255)
                                                                           aatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGc                                                                     >  2:4962026‑M2/6‑151 (MQ=255)
                                                                            atggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCa                                                                    <  2:10127823‑M2/147‑1 (MQ=255)
                                                                             tggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAg                                                                   <  1:6508879‑M2/148‑1 (MQ=255)
                                                                              ggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGt                                                                  <  2:2390065‑M2/149‑1 (MQ=255)
                                                                               gCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTg                                                                 <  2:3220495‑M2/150‑1 (MQ=255)
                                                                                cgcgGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCACCCTGGCGAGGAAGTCGCAGTGc                                                                >  2:6932129/1‑151 (MQ=255)
                                                                                      ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgcg                                                           <  2:7965480/144‑1 (MQ=255)
                                                                                      ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgcg                                                           <  1:7818015/144‑1 (MQ=255)
                                                                                        ccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCggg                                                         <  2:5785529/146‑1 (MQ=255)
                                                                                         cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGt                                                        <  2:834652/147‑1 (MQ=255)
                                                                                         cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGt                                                        >  2:4646599/5‑151 (MQ=255)
                                                                                           cgccGCGGCGGCCCCGCACGCGCTAACTCCAGAGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGACGGCCATCGGCAACCTGGCGAGGAAGACGCAGTGCTCGCGGGTCa                                                      >  2:6220099/3‑151 (MQ=255)
                                                                                            gccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAg                                                     <  1:6902843/150‑1 (MQ=255)
                                                                                             ccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGc                                                    >  1:8474710/1‑151 (MQ=255)
                                                                                              cgcggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGcc                                                   >  1:7494348/7‑151 (MQ=255)
                                                                                              cgcggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGcc                                                   <  2:7769957/145‑1 (MQ=255)
                                                                                               gcggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCg                                                  >  1:4919096/6‑151 (MQ=255)
                                                                                               gcggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCg                                                  <  1:3010149/146‑1 (MQ=255)
                                                                                                cggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCgg                                                 >  1:510348/5‑151 (MQ=255)
                                                                                                    ggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCa                                             >  1:9068799/1‑151 (MQ=255)
                                                                                                          gCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCt                                       <  1:9190847/151‑1 (MQ=255)
                                                                                                            aCGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTg                                     >  2:6294714/1‑151 (MQ=255)
                                                                                                             cgcgCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGc                                    <  2:10013966/151‑1 (MQ=255)
                                                                                                             cgcgCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGc                                    <  2:2084689/151‑1 (MQ=255)
                                                                                                              gcgcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGcc                                   <  2:5434961/151‑1 (MQ=255)
                                                                                                                gcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCtt                                 <  1:8850591/151‑1 (MQ=255)
                                                                                                                  tCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTcc                               <  1:3857535/151‑1 (MQ=255)
                                                                                                                   cACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcg                              <  1:5401572/151‑1 (MQ=255)
                                                                                                                    aCTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcgc                             <  1:2465907/151‑1 (MQ=255)
                                                                                                                     cTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcgcg                            >  1:7738681/1‑151 (MQ=255)
                                                                                                                      tCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCgcgc                           >  2:1543972/1‑151 (MQ=255)
                                                                                                                        cAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGccc                         <  2:9460405/151‑1 (MQ=255)
                                                                                                                         aGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCa                        <  2:8907244/151‑1 (MQ=255)
                                                                                                                          gCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCAt                       <  1:1040722/151‑1 (MQ=255)
                                                                                                                            ggggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCCTCGGCAACCTGGCGAGGAAGGCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCGCATCa                     >  2:1818680/1‑151 (MQ=255)
                                                                                                                            ggggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCa                     >  1:4793039/1‑151 (MQ=255)
                                                                                                                             gggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCAc                    <  2:7285920/151‑1 (MQ=255)
                                                                                                                             gggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTGCCGCGCCCATCAc                    >  2:2245056/1‑151 (MQ=255)
                                                                                                                              ggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCAcc                   <  1:6341842/151‑1 (MQ=255)
                                                                                                                               gTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCa                  <  2:656531/151‑1 (MQ=255)
                                                                                                                                tGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCAcagc                 >  2:2699387/1‑148 (MQ=255)
                                                                                                                                  cgcgGGCGGCCGATCCCGCACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAACGGCAACCTCCCGAGGAAGTCGCAGTGCTCGCGGGACAGCCGGCGCATGCCCGTGCCTACCGCGCCCATCACCAGCa               <  2:7911761/151‑1 (MQ=255)
                                                                                                                                  cgcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCa               <  2:3857194/151‑1 (MQ=255)
                                                                                                                                  cgcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCa               <  1:8010443/151‑1 (MQ=255)
                                                                                                                                   gcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCAc              <  2:4008285/151‑1 (MQ=255)
                                                                                                                                   gcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCAc              >  1:2200471/1‑151 (MQ=255)
                                                                                                                                    cTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACg             >  1:1134963/1‑151 (MQ=255)
                                                                                                                                      ggCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGt           <  1:10079091/151‑1 (MQ=255)
                                                                                                                                         ggaggaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCgg        <  2:5264826/151‑1 (MQ=255)
                                                                                                                                           aggaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGcc      >  1:7051099/1‑151 (MQ=255)
                                                                                                                                              aTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCg   <  1:311211/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCGT  >  NC_008463/5809219‑5809512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑