Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,649,107 A→G N212S (AAC→AGC)  PA14_RS12475 → TonB‑dependent receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,649,1070AG100.0% 1660.0 / NA 440N212S (AAC→AGC) PA14_RS12475TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (233/207);  total (233/207)

Only 100 of 440 total aligned reads displayed.

CGCAGCAGCGGCGCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAACGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTATGGTTC  >  NC_008463/2648959‑2649254
                                                                                                                                                    |                                                                                                                                                   
   agcagcGGCGCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTAGGCGGTGACCCACACGCCGGAGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCa                                                                                                                                                >  1:2806408/1‑151 (MQ=255)
     cagcGGCGCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAg                                                                                                                                              >  2:8830272/1‑151 (MQ=255)
      agcGGCGCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGc                                                                                                                                             <  1:6854477/151‑1 (MQ=255)
      agcGGCGCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGc                                                                                                                                             >  2:6205178/1‑151 (MQ=255)
         ggcgCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTc                                                                                                                                          >  1:8590439/1‑151 (MQ=255)
         ggcgCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTc                                                                                                                                          <  1:4142207/151‑1 (MQ=255)
            gcgGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCGCGGCGGTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCACGCGTCGcc                                                                                                                                       >  1:10626651/1‑151 (MQ=255)
              ggTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCAc                                                                                                                                     >  2:8348921/1‑151 (MQ=255)
              ggTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCAc                                                                                                                                     >  2:5600192/1‑151 (MQ=255)
                tCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGa                                                                                                                                   <  2:2064291/151‑1 (MQ=255)
                    ccTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCGGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACg                                                                                                                               >  1:2635099/1‑151 (MQ=255)
                    ccTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGGTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACg                                                                                                                               >  2:8687478/1‑151 (MQ=255)
                     cTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACgg                                                                                                                              >  2:1403571/1‑151 (MQ=255)
                          cgATGCACTGGTCTAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACTCGCAGTCGCTGCGCGGGCTGATCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAAt                                                                                                                         <  2:10384835/151‑1 (MQ=255)
                          cgATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAAt                                                                                                                         <  2:4644775/151‑1 (MQ=255)
                          cgATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAAt                                                                                                                         <  1:23703/151‑1 (MQ=255)
                           gATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAAtc                                                                                                                        >  1:3132732/1‑151 (MQ=255)
                            aTGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTGGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGTGCGGCCAGCGTCGCCACGAAACGGCCCAtct                                                                                                                       >  1:5922214/1‑151 (MQ=255)
                             tGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAAtctc                                                                                                                      >  2:9519937/1‑151 (MQ=255)
                             tGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAAtctc                                                                                                                      >  1:2468938/1‑151 (MQ=255)
                                aCTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAat                                                                                                                   <  1:8563040/151‑1 (MQ=255)
                                aCTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAat                                                                                                                   >  2:2613603/1‑151 (MQ=255)
                                   ggTCAAGCTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCaatctcaatctc                                                                                                                >  2:7729141/1‑151 (MQ=255)
                                   ggTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATc                                                                                                                >  2:3224098/1‑151 (MQ=255)
                                   ggTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGGCGCGGCGCGGGCTGAGCCCCAACCATGGGCTGGTGCTGGTGCGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATc                                                                                                                >  1:8844265/1‑151 (MQ=255)
                                    gTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCa                                                                                                               >  2:8083756/1‑151 (MQ=255)
                                      cAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGc                                                                                                             <  1:7339856/151‑1 (MQ=255)
                                      cAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGc                                                                                                             <  1:2151555/151‑1 (MQ=255)
                                       aaaCTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCg                                                                                                            <  1:8040004/151‑1 (MQ=255)
                                        aaCTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCgg                                                                                                           <  2:7282526/151‑1 (MQ=255)
                                         aCTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGTGCGGCAAGCGTCGCCACGAAAAGGCCAAACTCAATATCAGCggg                                                                                                          >  1:9940561/1‑151 (MQ=255)
                                          ctgctgCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCgggg                                                                                                         >  2:1584637/1‑151 (MQ=255)
                                            gctgcCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCgggggg                                                                                                       <  1:9376919/151‑1 (MQ=255)
                                            gctgcCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCgggggg                                                                                                       >  2:7853881/1‑151 (MQ=255)
                                              tgcCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCCGTCGCTGCGCGGGCTGAGCCCCACCCATGTGCTGGTGCTGGTGAGCGGCAATCGTCGCCACGAATCGGCCAATCTCAATATCAGCGGGGGGCt                                                                                                     <  1:7100242/151‑1 (MQ=255)
                                              tgcCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCt                                                                                                     >  2:287306/1‑151 (MQ=255)
                                                  cTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGgag                                                                                                 <  1:8086215/151‑1 (MQ=255)
                                                   tCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGgaga                                                                                                >  2:10537674/1‑151 (MQ=255)
                                                    cGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCGGAGCCCCAAACCAGTGGGGGTGCTGGTGTGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGaa                                                                                               >  1:10061365/1‑151 (MQ=255)
                                                     gCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAg                                                                                              <  2:8997505/151‑1 (MQ=255)
                                                     gCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAAGATCAGCGGGGGGCTGGAGAAg                                                                                              >  2:2206617/1‑151 (MQ=255)
                                                        gACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGc                                                                                           >  2:4382993/1‑151 (MQ=255)
                                                         aCCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCt                                                                                          >  1:4455078/1‑151 (MQ=255)
                                                         aCCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGGTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGGGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAggggg                                                                                          >  1:6550799/1‑149 (MQ=255)
                                                           ccGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTcc                                                                                        >  1:10200679/1‑151 (MQ=255)
                                                           ccGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTcc                                                                                        <  1:1808189/151‑1 (MQ=255)
                                                            cGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCa                                                                                       <  2:9173646/151‑1 (MQ=255)
                                                                        ccGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGACGGGCTCCACCGGCGTCGATc                                                                           >  2:4032893/1‑151 (MQ=255)
                                                                            tCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGa                                                                       <  1:5408476/151‑1 (MQ=255)
                                                                             cAACGCCTCGGCGATGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGac                                                                      <  2:6464300/151‑1 (MQ=255)
                                                                              aaCGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGaca                                                                     >  2:9868215/1‑151 (MQ=255)
                                                                                 gCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCa                                                                  <  1:1931083/151‑1 (MQ=255)
                                                                                 gCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCa                                                                  >  2:683968/1‑151 (MQ=255)
                                                                                  ccTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCAt                                                                 >  1:7898993/1‑151 (MQ=255)
                                                                                  ccTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCAt                                                                 >  1:3945/1‑151 (MQ=255)
                                                                                   cTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATc                                                                >  2:4461371/1‑151 (MQ=255)
                                                                                   cTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATc                                                                >  1:5136254/1‑151 (MQ=255)
                                                                                    tCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATcc                                                               <  2:3748225/151‑1 (MQ=255)
                                                                                      ggCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCg                                                             >  2:492136/1‑151 (MQ=255)
                                                                                      ggCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCg                                                             >  1:2318728/1‑151 (MQ=255)
                                                                                         gTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATc                                                          <  2:3036823/151‑1 (MQ=255)
                                                                                         gTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATc                                                          >  1:3597037/1‑151 (MQ=255)
                                                                                         gTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATATGGCCACCATCCCGATc                                                          >  2:6060005/1‑151 (MQ=255)
                                                                                                 acacGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCAt                                                  >  1:4551569/1‑151 (MQ=255)
                                                                                                    cGCAGTCGCTGCGCGGGCTGAGCCCCAACCCTGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGa                                               <  2:4592136/151‑1 (MQ=255)
                                                                                                       aGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACCCCATCCCGATCAACGCCATCGAGCg                                            >  2:10312655/1‑151 (MQ=255)
                                                                                                       aGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCg                                            >  1:5287854/1‑151 (MQ=255)
                                                                                                        gTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCgg                                           >  1:764674/1‑151 (MQ=255)
                                                                                                          cGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGt                                         >  1:6956645/1‑151 (MQ=255)
                                                                                                           gCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTc                                        <  1:8183850/151‑1 (MQ=255)
                                                                                                             tGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGa                                      <  2:8246807/151‑1 (MQ=255)
                                                                                                              gcgcGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAg                                     >  1:3419963/1‑151 (MQ=255)
                                                                                                               cgcgGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAgg                                    >  2:2390754/1‑151 (MQ=255)
                                                                                                                gcgGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGt                                   <  2:8554152/151‑1 (MQ=255)
                                                                                                                 cgGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGtg                                  >  1:484969/1‑151 (MQ=255)
                                                                                                                 cgGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGAACAACGCCATCGAGCGGGTCGAGGtg                                  <  1:1482535/151‑1 (MQ=255)
                                                                                                                  gggCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGtgc                                 <  2:10623270/151‑1 (MQ=255)
                                                                                                                   ggCTGAGCCCCAACCCTGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGtgct                                <  2:7085330/151‑1 (MQ=255)
                                                                                                                   ggCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGCTCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGtgct                                >  1:7539082/1‑151 (MQ=255)
                                                                                                                    gCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGtgctg                               <  2:5922903/151‑1 (MQ=255)
                                                                                                                    gCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGtgctg                               >  2:7026275/1‑151 (MQ=255)
                                                                                                                           cccAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGAGCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACg                        >  1:4922857/1‑151 (MQ=255)
                                                                                                                            ccAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACgg                       >  2:1134018/1‑151 (MQ=255)
                                                                                                                             cAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGt                      <  1:2432836/151‑1 (MQ=255)
                                                                                                                              aaCCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTg                     <  1:3033843/151‑1 (MQ=255)
                                                                                                                                ccATGTGCTGGTGCTGGAGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCt                   <  2:1068497/151‑1 (MQ=255)
                                                                                                                                 cctgtgCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAAGATCAGCGGGGGGCTGGAGAAGGGCTCCCCCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCtt                  <  2:346031/149‑1 (MQ=255)
                                                                                                                                  aTGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTc                 >  2:5224204/1‑151 (MQ=255)
                                                                                                                                  aTGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTc                 >  2:3158767/1‑151 (MQ=255)
                                                                                                                                  aTGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAAACTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTc                 <  1:3288470/151‑1 (MQ=255)
                                                                                                                                    gtgctggtgctgGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCgg               <  1:10813969/151‑1 (MQ=255)
                                                                                                                                    gtgctggtgctgGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCgg               >  2:9749157/1‑151 (MQ=255)
                                                                                                                                    gagctggtgctggtGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCgg               <  2:1810543/149‑1 (MQ=255)
                                                                                                                                     tgctggtgctggTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGc              <  2:6977231/151‑1 (MQ=255)
                                                                                                                                      gctggtgctggtGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATCTCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGca             <  1:8478945/151‑1 (MQ=255)
                                                                                                                                       ctggtgctggtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATCTCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGcac            <  2:9546089/151‑1 (MQ=255)
                                                                                                                                       ctggtgctggtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGcac            >  2:5284018/1‑151 (MQ=255)
                                                                                                                                        tggtgctggtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGcaca           >  1:6215919/1‑151 (MQ=255)
                                                                                                                                               ggtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTATGGt    >  2:7540474/1‑151 (MQ=255)
                                                                                                                                                 tgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTATGGTTc  <  1:8346530/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCAGCAGCGGCGCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAACGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTATGGTTC  >  NC_008463/2648959‑2649254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑