Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 888,038 2 bp→TG coding (1562‑1563/1878 nt) PA14_RS04165 → sigma‑54‑dependent Fis family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463888,0380CT99.7% 2484.0 / NA 600P521M (CCT→CTG) PA14_RS04165sigma‑54‑dependent Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (305/293);  minor base G (2/0);  total (307/293)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_008463888,0390TG100.0% 2341.4 / NA 600P521M (CCT→CTG) PA14_RS04165sigma‑54‑dependent Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (307/293);  total (307/293)

Only 100 of 600 total aligned reads displayed.

GAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCCTGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGCC  >  NC_008463/887893‑888183
                                                                                                                                                 ||                                                                                                                                                
 aGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATg                                                                                                                                             >  2:6330068/1‑151 (MQ=255)
   gATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGcc                                                                                                                                           >  2:4149260/1‑151 (MQ=255)
   gATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGAACGCATCGAACTCGGCGAGGCAGCCCTGGAATGcc                                                                                                                                           >  1:4674333/1‑151 (MQ=255)
      ctctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGc                                                                                                                                        >  2:5483875/1‑151 (MQ=255)
       tctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCt                                                                                                                                       >  1:569281/1‑151 (MQ=255)
       tctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCt                                                                                                                                       <  2:178252/151‑1 (MQ=255)
       tctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCt                                                                                                                                       <  2:5802281/151‑1 (MQ=255)
          aCTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCgg                                                                                                                                    >  1:9115661/1‑151 (MQ=255)
          aCTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCgg                                                                                                                                    >  2:5696044/1‑151 (MQ=255)
            ttCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCt                                                                                                                                  >  2:8629217/1‑151 (MQ=255)
            ttCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCt                                                                                                                                  <  2:6665994/151‑1 (MQ=255)
              ccGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTAt                                                                                                                                >  1:1374354/1‑151 (MQ=255)
              ccGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTAt                                                                                                                                >  1:4429044/1‑151 (MQ=255)
               cGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGAGGAGGAAACCGCGCACTGCGGCGTACGCATCGTCCTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATc                                                                                                                               >  2:8392207/1‑151 (MQ=255)
               cGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATc                                                                                                                               <  1:3469479/151‑1 (MQ=255)
               cGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATc                                                                                                                               <  2:8128131/151‑1 (MQ=255)
               cGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATc                                                                                                                               >  1:8824487/1‑151 (MQ=255)
                gCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCg                                                                                                                              <  2:596968/151‑1 (MQ=255)
                 ccTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGc                                                                                                                             >  1:10738353/1‑151 (MQ=255)
                 ccTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGc                                                                                                                             >  1:9416156/1‑151 (MQ=255)
                  cTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCt                                                                                                                            >  1:4992788/1‑151 (MQ=255)
                     ggcggcGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGc                                                                                                                         >  2:4754613/1‑151 (MQ=255)
                       cggcgCCCGCTTCGACCTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGAGCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCg                                                                                                                       <  2:3445994/151‑1 (MQ=255)
                          cgcCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGc                                                                                                                    >  2:10667213/1‑151 (MQ=255)
                           gcCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCa                                                                                                                   <  2:9756165/151‑1 (MQ=255)
                           gcCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCa                                                                                                                   >  1:2479274/1‑151 (MQ=255)
                             ccGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCact                                                                                                                 >  1:9020264/1‑149 (MQ=255)
                                cTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCCAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTc                                                                                                              <  1:10011153/151‑1 (MQ=255)
                                 ttCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCACTGTcc                                                                                                             >  2:701065/1‑151 (MQ=255)
                                    gAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGcc                                                                                                          >  2:2996870/1‑151 (MQ=255)
                                     aaCTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACACGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCa                                                                                                         >  1:5250801/1‑151 (MQ=255)
                                         gccgccGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTg                                                                                                     <  2:7663815/151‑1 (MQ=255)
                                         gccgccGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTg                                                                                                     <  2:10076619/151‑1 (MQ=255)
                                           cgccgcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCg                                                                                                   <  2:10787529/151‑1 (MQ=255)
                                              cgcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCa                                                                                                <  1:7210118/151‑1 (MQ=255)
                                              cgcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCa                                                                                                >  2:4672756/1‑151 (MQ=255)
                                              cgcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCa                                                                                                >  1:8119990/1‑151 (MQ=255)
                                              cgcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGACGGGCAATGTCCGCCAGTTGCGGCa                                                                                                >  1:76845/1‑151 (MQ=255)
                                               gcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAt                                                                                               <  2:2326485/151‑1 (MQ=255)
                                                     cgAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTg                                                                                         <  1:6289942/151‑1 (MQ=255)
                                                     cgAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTg                                                                                         >  2:9491829/1‑151 (MQ=255)
                                                     cgAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTg                                                                                         <  1:8735320/151‑1 (MQ=255)
                                                      gAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTgc                                                                                        >  2:4254469/1‑151 (MQ=255)
                                                       aaCGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCATTTGCGGCATGTGTTgcg                                                                                       <  2:8324622/151‑1 (MQ=255)
                                                       aaCGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTgcg                                                                                       <  2:2886823/151‑1 (MQ=255)
                                                       aaCGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGA‑GAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTgcgc                                                                                      >  1:7569933/1‑151 (MQ=255)
                                                         cGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCt                                                                                     >  2:6133063/1‑151 (MQ=255)
                                                          gCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTa                                                                                    <  1:3748598/151‑1 (MQ=255)
                                                            aGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCTATGTCCGCCAGTTGCGGCATGTGTTGCGCTACg                                                                                  <  1:5943405/151‑1 (MQ=255)
                                                                  cGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgcg                                                                            >  2:2723149/1‑151 (MQ=255)
                                                                   ggCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGACTGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgcgc                                                                           >  2:1666433/1‑151 (MQ=255)
                                                                    gCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgcgcg                                                                          <  1:9498489/151‑1 (MQ=255)
                                                                      tGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCTGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCTGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCt                                                                        <  2:2008700/151‑1 (MQ=255)
                                                                      tGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCt                                                                        >  2:7599594/1‑151 (MQ=255)
                                                                       ggCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCtg                                                                       >  1:7403026/1‑151 (MQ=255)
                                                                        gcgcTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCCATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCtgt                                                                      >  2:4331864/1‑151 (MQ=255)
                                                                        gcgcTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCtgt                                                                      >  1:8573023/1‑151 (MQ=255)
                                                                        gcgcTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCtgt                                                                      <  1:4244091/151‑1 (MQ=255)
                                                                        gcgcTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCtgt                                                                      >  1:3573364/1‑151 (MQ=255)
                                                                            tGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcgg                                                                  <  1:4001409/151‑1 (MQ=255)
                                                                             gATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggc                                                                 <  1:3223663/151‑1 (MQ=255)
                                                                             gATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggc                                                                 <  1:1506700/151‑1 (MQ=255)
                                                                              aTCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcg                                                                >  2:4626078/1‑151 (MQ=255)
                                                                              aTCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcg                                                                <  1:8223240/151‑1 (MQ=255)
                                                                                 cGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcggcg                                                             >  1:10577812/1‑151 (MQ=255)
                                                                                         ccTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTg                                                     <  2:679603/151‑1 (MQ=255)
                                                                                          cTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGc                                                    >  2:2563841/1‑151 (MQ=255)
                                                                                          cTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGc                                                    >  1:2993201/1‑151 (MQ=255)
                                                                                           tCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCa                                                   >  1:2791925/1‑151 (MQ=255)
                                                                                             gacgaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAAc                                                 >  2:8483559/1‑151 (MQ=255)
                                                                                              acgaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACt                                                >  1:9668929/1‑151 (MQ=255)
                                                                                              acgaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACt                                                >  1:8861874/1‑151 (MQ=255)
                                                                                               cgaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCGACTc                                               >  2:921343/1‑151 (MQ=255)
                                                                                               cgaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTc                                               <  2:4956594/151‑1 (MQ=255)
                                                                                                gaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCg                                              >  1:10144435/1‑151 (MQ=255)
                                                                                                gaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCg                                              >  2:3217512/1‑151 (MQ=255)
                                                                                                 aGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGc                                             >  2:8962520/1‑151 (MQ=255)
                                                                                                 aGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGc                                             >  1:5430506/1‑151 (MQ=255)
                                                                                                  ggAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCTGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGcc                                            <  2:577428/151‑1 (MQ=255)
                                                                                                  ggAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGcc                                            <  2:1456789/151‑1 (MQ=255)
                                                                                                   gAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCg                                           >  2:4000728/1‑151 (MQ=255)
                                                                                                     aaCCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGAc                                         >  1:2148029/1‑151 (MQ=255)
                                                                                                       ccGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCt                                       <  2:4188023/151‑1 (MQ=255)
                                                                                                         gcgcACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTc                                     >  2:10255271/1‑151 (MQ=255)
                                                                                                           gcACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCg                                   >  1:6531583/1‑151 (MQ=255)
                                                                                                           gcACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCg                                   <  1:9609500/151‑1 (MQ=255)
                                                                                                           gcACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCAGCTCGGCTATCGCAGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCAACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCg                                   >  2:3727134/1‑151 (MQ=255)
                                                                                                            cACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCgg                                  >  2:653720/1‑151 (MQ=255)
                                                                                                            cACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCgg                                  >  1:10422878/1‑151 (MQ=255)
                                                                                                             aCTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGc                                 >  1:6935147/1‑151 (MQ=255)
                                                                                                                 cggcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCagag                             <  1:7913690/151‑1 (MQ=255)
                                                                                                                  ggcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGc                            <  2:2236903/151‑1 (MQ=255)
                                                                                                                     gTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGc                         >  2:7206142/1‑151 (MQ=255)
                                                                                                                          cATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGc                    >  2:9666691/1‑151 (MQ=255)
                                                                                                                                aCTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGc              >  1:1415102/1‑151 (MQ=255)
                                                                                                                                 cTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGcc             <  1:6731359/151‑1 (MQ=255)
                                                                                                                                 cTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCGATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGcc             <  2:5181265/151‑1 (MQ=255)
                                                                                                                                      cGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGaca        <  1:1814803/151‑1 (MQ=255)
                                                                                                                                           cAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGc   >  2:7950466/1‑151 (MQ=255)
                                                                                                                                            aGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGcc  >  1:6420468/1‑151 (MQ=255)
                                                                                                                                                 ||                                                                                                                                                
GAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCCTGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGCC  >  NC_008463/887893‑888183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑