BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	155,993	Δ1 bp	coding (922/981 nt)	PA14_RS00700 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	155,990	0	G	.	99.8%	2426.7 / ‑3.7	491	coding (919/981 nt)	PA14_RS00700	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (146/344); minor base A (1/0); total (147/344)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 514 total aligned reads displayed.

TGGCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCGGGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAACA  >  NC_008463/155841‑156135

tggCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCg                                                                                                                                                   >  1:10670736/1‑150 (MQ=255)

    ccAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCgggca                                                                                                                                               <  1:1748731/150‑3 (MQ=255)

     cAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCgggcac                                                                                                                                              <  1:7646988/150‑4 (MQ=255)

     cAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCgggcac                                                                                                                                              <  2:1570652/150‑4 (MQ=255)

      aGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCgggcact                                                                                                                                             <  1:3135613/150‑5 (MQ=255)

        gggATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGa                                                                                                                                          <  2:7573007/150‑1 (MQ=255)

        gggATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGa                                                                                                                                          <  2:2131307/150‑1 (MQ=255)

              gggCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAggg                                                                                                                                    <  2:6213241/150‑1 (MQ=255)

              gggCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAggg                                                                                                                                    <  1:1499442/150‑1 (MQ=255)

                gCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCa                                                                                                                                  <  2:5300282/150‑1 (MQ=255)

                   gTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGt                                                                                                                               <  2:10911057/150‑1 (MQ=255)

                   gTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGt                                                                                                                               <  2:6365290/150‑1 (MQ=255)

                     cGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttc                                                                                                                             <  1:8216083/150‑1 (MQ=255)

                     cGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTAGGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttc                                                                                                                             <  2:7334358/150‑1 (MQ=255)

                       aTTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttctt                                                                                                                           <  2:743552/150‑1 (MQ=255)

                          ccGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGa                                                                                                                        <  2:9046726/150‑1 (MQ=255)

                          ccGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGa                                                                                                                        >  1:5418664/1‑150 (MQ=255)

                           cGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGCCCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGATTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGTGGGCCGGTTCTACGAt                                                                                                                       >  2:4120156/1‑150 (MQ=255)

                           cGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGAt                                                                                                                       <  1:3333875/150‑1 (MQ=255)

                               cAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgt                                                                                                                   <  1:9160761/150‑1 (MQ=255)

                                 aCCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttg                                                                                                                 <  2:698190/150‑1 (MQ=255)

                                    tgctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGttt                                                                                                              <  2:4566136/150‑1 (MQ=255)

                                     gctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGtttt                                                                                                             >  2:9158402/1‑150 (MQ=255)

                                      ctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTa                                                                                                            <  1:2721118/150‑1 (MQ=255)

                                      ctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTa                                                                                                            <  1:6173441/150‑1 (MQ=255)

                                       tgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTaa                                                                                                           >  2:7805201/1‑150 (MQ=255)

                                         gCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACt                                                                                                         <  2:1179294/150‑1 (MQ=255)

                                          cGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTa                                                                                                        <  2:2424614/150‑1 (MQ=255)

                                          cGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGACAGCCGTGGAAGTCC‑GTGCACTGACGAGGGCAGGTTCTTCGATTAGTAGTTTTAACTa                                                                                                        >  1:5081904/1‑150 (MQ=255)

                                           gATGCTGCCCTATGCGGCGACCATCCTTGTGCTGGTCCTGCTCTCCCGCGAGGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAt                                                                                                       <  2:3790223/150‑1 (MQ=255)

                                           gATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAt                                                                                                       <  2:5368716/150‑1 (MQ=255)

                                            aTGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAtt                                                                                                      <  1:2012762/150‑1 (MQ=255)

                                             tgctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAttt                                                                                                     <  1:6505192/150‑1 (MQ=255)

                                              gctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTta                                                                                                    <  2:4709639/150‑1 (MQ=255)

                                               ctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtat                                                                                                   >  2:8748408/1‑150 (MQ=255)

                                                tgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtata                                                                                                  <  2:1497422/150‑1 (MQ=255)

                                                  cccTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtatata                                                                                                >  2:9517137/1‑150 (MQ=255)

                                                  cccTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGGATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtatata                                                                                                <  2:10729690/150‑1 (MQ=255)

                                                   ccTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTatatat                                                                                               <  2:10007643/150‑1 (MQ=255)

                                                    cTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAtt                                                                                              <  2:962288/150‑1 (MQ=255)

                                                    cTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAtt                                                                                              <  1:629097/150‑1 (MQ=255)

                                                    cTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGATGTTTAAACTATTTAAATAtt                                                                                              >  2:7946485/1‑150 (MQ=255)

                                                    cTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGAATAGTTGTGTTAACTATTTATATAtt                                                                                              >  2:6021259/1‑150 (MQ=255)

                                                      aTGCGGCGACCATCCTGGTGCTTGTCCTGCTCTCCCGCGATGCCCTGCTCACCAGGCTGTATGCCCCGGTTTCCCTGGGCCTGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTATTTGTTTTAACTATTTATATAttct                                                                                            <  1:10395796/150‑1 (MQ=255)

                                                      aTGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttct                                                                                            <  1:4973025/150‑1 (MQ=255)

                                                       tGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttctt                                                                                           >  1:3549323/1‑150 (MQ=255)

                                                        gcggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttcttc                                                                                          <  2:10436328/150‑1 (MQ=255)

                                                        gcggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttcttc                                                                                          <  1:6792593/150‑1 (MQ=255)

                                                         cggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCg                                                                                         <  2:11002793/150‑1 (MQ=255)

                                                         cggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAGTGATATATTCTTCg                                                                                         >  1:5820880/1‑150 (MQ=255)

                                                          ggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTCTGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGa                                                                                        <  2:6525979/150‑1 (MQ=255)

                                                              aCCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGa                                                                                    <  2:3581509/150‑1 (MQ=255)

                                                               ccATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGAt                                                                                   >  2:106177/1‑150 (MQ=255)

                                                                cATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGAtt                                                                                  >  2:5666984/1‑150 (MQ=255)

                                                                cATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGAtt                                                                                  <  2:1652792/150‑1 (MQ=255)

                                                                   ccTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAg                                                                               <  1:8400403/150‑1 (MQ=255)

                                                                   ccTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAg                                                                               <  1:10820256/150‑1 (MQ=255)

                                                                    cTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GTGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGa                                                                              <  1:9682840/150‑1 (MQ=255)

                                                                     tGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGAt                                                                             <  1:3897267/150‑1 (MQ=255)

                                                                      ggTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATa                                                                            <  2:2326114/150‑1 (MQ=255)

                                                                       gTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATaa                                                                           <  1:7076105/150‑1 (MQ=255)

                                                                         gCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAAtt                                                                         <  2:582665/150‑1 (MQ=255)

                                                                           tGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTc                                                                       <  1:9003112/150‑1 (MQ=255)

                                                                            ggTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCa                                                                      >  2:11120905/1‑150 (MQ=255)

                                                                             gTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCAc                                                                     >  1:10131952/1‑150 (MQ=255)

                                                                             gTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCAc                                                                     <  2:8846063/150‑1 (MQ=255)

                                                                                 tGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGGGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGAt                                                                 <  2:3295469/150‑1 (MQ=255)

                                                                                   ctctCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATgg                                                               >  2:2541517/1‑150 (MQ=255)

                                                                                   ctctCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATgg                                                               <  1:2277582/150‑1 (MQ=255)

                                                                                     ctcCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGcc                                                             >  2:936861/1‑150 (MQ=255)

                                                                                        ccGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTaa                                                          <  1:468295/150‑1 (MQ=255)

                                                                                         cgcgATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTaaa                                                         <  2:5183400/150‑1 (MQ=255)

                                                                                         cgcgATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTaaa                                                         <  2:7276794/150‑1 (MQ=255)

                                                                                          gcgATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAAt                                                        <  1:9866433/150‑1 (MQ=255)

                                                                                             aTGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCaa                                                     >  1:9517876/1‑150 (MQ=255)

                                                                                              tGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAAt                                                    <  2:7199972/150‑1 (MQ=255)

                                                                                                 ccTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATggg                                                 <  2:5503670/150‑1 (MQ=255)

                                                                                                  cTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATgggg                                                <  2:314792/150‑1 (MQ=255)

                                                                                                      gcACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCAtt                                            <  2:4998672/150‑1 (MQ=255)

                                                                                                       cACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCAttt                                           <  1:3836083/150‑1 (MQ=255)

                                                                                                       cACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCAttt                                           <  1:9380182/150‑1 (MQ=255)

                                                                                                        aCCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCAtttt                                          <  2:6644424/150‑1 (MQ=255)

                                                                                                           cGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGc                                       <  1:8630961/150‑1 (MQ=255)

                                                                                                             gCTGTAGGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTCGTGGTCTTAACGATTTATATCTTCGTCGATAGATTGAGCTAATTTCACTGATGGCCTAACTCAATGGGGCATTTTTGCCg                                     <  2:8148861/150‑1 (MQ=255)

                                                                                                               tGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGaa                                   <  1:1169114/150‑1 (MQ=255)

                                                                                                                 tATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAAcc                                 <  1:7913569/150‑1 (MQ=255)

                                                                                                                   tGCCCCGGTTACCCTGGGCCAGCCGTGGCAGGCC‑GGGCACAGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCGAAATCAATGGGGCATTTTTGCCGAAcccc                               <  1:8086889/150‑1 (MQ=255)

                                                                                                                      cccGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTCACTCAATGGGGCATTTTTGCCGAACCACGtt                            >  2:7648999/1‑150 (MQ=255)

                                                                                                                          gTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGa                        >  1:3484471/1‑150 (MQ=255)

                                                                                                                            ttCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGAtt                      >  1:5328709/1‑150 (MQ=255)

                                                                                                                                cTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTcc                  >  1:3968168/1‑150 (MQ=255)

                                                                                                                                 tGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTccc                 <  1:2495433/150‑1 (MQ=255)

                                                                                                                                 tGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAGTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTccc                 <  1:4410707/150‑1 (MQ=255)

                                                                                                                                   ggCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAg               <  1:1843681/150‑1 (MQ=255)

                                                                                                                                   ggCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAg               <  1:7434662/150‑1 (MQ=255)

                                                                                                                                    gCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGt              <  2:2634737/150‑1 (MQ=255)

                                                                                                                                        gCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGAt          >  2:865150/1‑150 (MQ=255)

                                                                                                                                        gCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGAt          >  2:437976/1‑150 (MQ=255)

                                                                                                                                          cGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGAttt        <  1:7313540/150‑1 (MQ=255)

                                                                                                                                          cGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGAttt        <  1:7195731/150‑1 (MQ=255)

TGGCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCGGGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAACA  >  NC_008463/155841‑156135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`