BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,791,973	Δ1 bp	intergenic (+67/‑68)	nadE → / → choE	ammonia‑dependent NAD(+) synthetase/cholinesterase ChoE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,791,973	0	A	.	100.0%	2835.3 / NA	571	intergenic (+67/‑68)	nadE/choE	ammonia‑dependent NAD(+) synthetase/cholinesterase ChoE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (294/277); total (294/277)

Only 100 of 571 total aligned reads displayed.

GCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCAACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTCAGCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAAAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCGCTGCT  >  NC_008463/5791828‑5792117

gcgcGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACCAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAAc                                                                                                                                             <  2:2517930/150‑1 (MQ=255)

  gcgAGCAAGCCGCGCGGGTGATCGTCGACACCAACCACAAGACCCACCACAAGCGCGAACAGCCGAAGGCGCCCCGAGCCTCCCGGCCCTTGAGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCa                                                                                                                                           <  2:6004414/150‑1 (MQ=255)

   cgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAg                                                                                                                                          <  2:2849237/150‑1 (MQ=255)

     aGGAAGCCGCGCGGGTGATCGTCGACACCTACCACATGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGt                                                                                                                                        <  2:633563/150‑1 (MQ=255)

     aGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGt                                                                                                                                        >  2:6173711/1‑150 (MQ=255)

     aGGAAGCCGCGCGGGTGATCGTCGACACCTACCAAAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCGTTGACGCGGGCGGGCAAGTGCCTGTC‑GCAACCAGGt                                                                                                                                        >  2:9260682/1‑150 (MQ=255)

         aGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGGCTGTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTc                                                                                                                                    >  1:4214408/1‑150 (MQ=255)

                cgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcg                                                                                                                             >  1:2080228/1‑150 (MQ=255)

                 gggTGATCGTCGACACCTACCACAACACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgc                                                                                                                            <  2:6913397/150‑1 (MQ=255)

                    tGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcgcg                                                                                                                         >  1:958818/1‑150 (MQ=255)

                    tGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcgcg                                                                                                                         <  1:8134304/150‑1 (MQ=255)

                     gATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcgg                                                                                                                        >  1:3649384/1‑150 (MQ=255)

                      aTCGTCGACACCTACCACCAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggc                                                                                                                       <  2:1649575/150‑1 (MQ=255)

                      aTCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggc                                                                                                                       <  2:1597286/150‑1 (MQ=255)

                          tcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcggcg                                                                                                                   <  1:5009025/150‑1 (MQ=255)

                            gACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGgcgcg                                                                                                                 >  1:6394812/1‑150 (MQ=255)

                             acacCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGgcgcgc                                                                                                                <  1:6438594/150‑1 (MQ=255)

                              cacCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCt                                                                                                               <  1:3416945/150‑1 (MQ=255)

                               acCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTa                                                                                                              >  1:4184020/1‑150 (MQ=255)

                               acCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTa                                                                                                              >  1:9745760/1‑150 (MQ=255)

                                 cTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTaga                                                                                                            >  1:4060681/1‑148 (MQ=255)

                                 cTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTaga                                                                                                            >  1:8908939/1‑148 (MQ=255)

                                  tACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTagaa                                                                                                           >  1:457757/1‑150 (MQ=255)

                                   aCCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAg                                                                                                          >  2:8652314/1‑150 (MQ=255)

                                    ccACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAgg                                                                                                         >  2:9609767/1‑150 (MQ=255)

                                     cacaAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCGAGAAGGa                                                                                                        >  2:3004537/1‑150 (MQ=255)

                                      acaAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGaa                                                                                                       >  1:4367980/1‑150 (MQ=255)

                                       cacgACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGCGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGTAg                                                                                                      <  2:10257221/147‑1 (MQ=255)

                                       caAGACCCCCCACCCGCGCGAACTGCCGAAGGCGCCCAGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCCGGTCTTCAAGTCCGCGCGGCGGCGCGCTAgaaggaag                                                                                                      <  2:5468018/150‑1 (MQ=255)

                                           aCCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGa                                                                                                  <  1:5352046/150‑1 (MQ=255)

                                            cccAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGaa                                                                                                 <  2:7698517/150‑1 (MQ=255)

                                            cccAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGaa                                                                                                 >  2:1963775/1‑150 (MQ=255)

                                               aGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATcc                                                                                              <  1:9105842/150‑1 (MQ=255)

                                               aGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATcc                                                                                              >  1:1778361/1‑150 (MQ=255)

                                                gCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATccc                                                                                             <  1:10462052/150‑1 (MQ=255)

                                                 cacaCGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCt                                                                                            <  2:4017553/150‑1 (MQ=255)

                                                 cacaAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCt                                                                                            <  1:6764493/150‑1 (MQ=255)

                                                 cacaAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCt                                                                                            <  1:3889404/150‑1 (MQ=255)

                                                 cacaAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCt                                                                                            <  1:9368151/150‑1 (MQ=255)

                                                   caAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGc                                                                                          >  2:3292557/1‑150 (MQ=255)

                                                        gcgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAgg                                                                                     >  2:257600/1‑150 (MQ=255)

                                                        gcgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAgg                                                                                     <  1:7266868/150‑1 (MQ=255)

                                                        gcgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAgg                                                                                     <  2:10556589/150‑1 (MQ=255)

                                                         cgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgc                                                                                    <  2:9418590/150‑1 (MQ=255)

                                                          gAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGACGGAAGCTGAATCCCTGCGAAGgcc                                                                                   >  1:8689204/1‑150 (MQ=255)

                                                             cTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccagc                                                                                >  2:7223875/1‑150 (MQ=255)

                                                             cTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccagc                                                                                <  1:9652452/150‑1 (MQ=255)

                                                              tGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccagcc                                                                               <  2:4293318/150‑1 (MQ=255)

                                                               gCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccagcca                                                                              >  2:1186627/1‑150 (MQ=255)

                                                               gCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCGTTGTCGCGGGCGGGACTGTGCCTGTC‑GCAACCAGGGCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccagcca                                                                              >  2:85077/1‑150 (MQ=255)

                                                                 cGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCTAGTCCGCGCGGCGGCGCGCTAGAAGGACGCTGAATCCCTGCGAAGGCCAGCCATg                                                                            >  2:2734865/1‑150 (MQ=255)

                                                                  gAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgc                                                                           <  1:7828952/150‑1 (MQ=255)

                                                                  gAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgc                                                                           <  1:5969133/150‑1 (MQ=255)

                                                                   aaGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgcg                                                                          >  2:5125106/1‑150 (MQ=255)

                                                                   aaGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgcg                                                                          >  2:7612044/1‑150 (MQ=255)

                                                                        gcCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCAccc                                                                     <  1:2379205/150‑1 (MQ=255)

                                                                        gcCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCAccc                                                                     <  1:7016004/150‑1 (MQ=255)

                                                                          ccTGAGCCTCCCGGCCCGTTCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGt                                                                   >  1:6527852/1‑150 (MQ=255)

                                                                            tGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtct                                                                 >  1:3261826/1‑150 (MQ=255)

                                                                            tGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtct                                                                 <  2:8864195/150‑1 (MQ=255)

                                                                               gCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTccc                                                              <  2:4777400/150‑1 (MQ=255)

                                                                                 cTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCg                                                            >  2:7408037/1‑150 (MQ=255)

                                                                                   cccGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGcc                                                          <  1:6200977/150‑1 (MQ=255)

                                                                                   cccGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCGAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGcc                                                          >  2:8264267/1‑150 (MQ=255)

                                                                                    ccGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGccc                                                         <  2:7880682/150‑1 (MQ=255)

                                                                                    ccGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGccc                                                         <  2:5259873/150‑1 (MQ=255)

                                                                                          cGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcc                                                   >  1:1175259/1‑150 (MQ=255)

                                                                                          cGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcc                                                   >  1:7527025/1‑150 (MQ=255)

                                                                                          cGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcc                                                   <  1:1930713/150‑1 (MQ=255)

                                                                                           gTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGAGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcct                                                  <  1:9842338/150‑1 (MQ=255)

                                                                                            tGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcctc                                                 >  2:6759506/1‑150 (MQ=255)

                                                                                             gCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCg                                                >  1:4681067/1‑150 (MQ=255)

                                                                                              cGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCgg                                               <  1:9991800/150‑1 (MQ=255)

                                                                                               ggCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGgt                                              >  1:3068674/1‑150 (MQ=255)

                                                                                                 cAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGgtgt                                            <  2:9098124/150‑1 (MQ=255)

                                                                                                   ggCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTcc                                          <  1:6031335/150‑1 (MQ=255)

                                                                                                    gCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCct                                         >  2:185110/1‑150 (MQ=255)

                                                                                                      ccGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctgc                                       >  2:10960669/1‑150 (MQ=255)

                                                                                                       cgtcgtCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctgct                                      >  1:9268791/1‑150 (MQ=255)

                                                                                                         tcgtcgACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTgg                                    >  2:4593796/1‑150 (MQ=255)

                                                                                                          cgtcGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggc                                   <  2:3724594/150‑1 (MQ=255)

                                                                                                          cgtcGACGGGCCATTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggc                                   <  1:10694653/150‑1 (MQ=255)

                                                                                                           gtcGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCCGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggcc                                  <  2:5704540/150‑1 (MQ=255)

                                                                                                           gtcGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggcc                                  >  1:2747138/1‑150 (MQ=255)

                                                                                                           gtcGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggcc                                  <  2:3603821/150‑1 (MQ=255)

                                                                                                             cgaCGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggccgg                                >  1:576552/3‑150 (MQ=255)

                                                                                                             cgaCGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggccgg                                >  1:8313944/3‑150 (MQ=255)

                                                                                                              gaCGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggccggc                               <  2:8338836/149‑1 (MQ=255)

                                                                                                                cGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCa                             <  1:7469374/150‑1 (MQ=255)

                                                                                                                 gggCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAg                            >  1:5591521/1‑150 (MQ=255)

                                                                                                                     cTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCt                        <  1:10294761/150‑1 (MQ=255)

                                                                                                                     cTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCt                        >  1:1021869/1‑150 (MQ=255)

                                                                                                                         gTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCgg                    <  1:6954444/150‑1 (MQ=255)

                                                                                                                            gcgggcggGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGaca                 >  2:8143384/1‑150 (MQ=255)

                                                                                                                                     aaTGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTcccc        >  1:10007698/1‑150 (MQ=255)

                                                                                                                                       tgtgCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCgc      <  2:9492533/150‑1 (MQ=255)

                                                                                                                                        gtgCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCgct     >  1:10979202/1‑150 (MQ=255)

                                                                                                                                        gtgCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCgct     >  1:1570084/1‑150 (MQ=255)

                                                                                                                                          gCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCgctgc   >  1:8489122/1‑150 (MQ=255)

                                                                                                                                           ccTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCgctgct  <  2:8889467/150‑1 (MQ=255)

GCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCAACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTCAGCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAAAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCGCTGCT  >  NC_008463/5791828‑5792117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`