BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	1,239,163	(C)_5→4	intergenic (+161/‑32)	PA14_RS05795 → / → lptF	alpha/beta hydrolase/LPS export ABC transporter permease LptF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	1,239,159	0	C	.	100.0%	2142.7 / NA	431	intergenic (+157/‑36)	PA14_RS05795/lptF	alpha/beta hydrolase/LPS export ABC transporter permease LptF
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (217/214); total (217/214)

Only 100 of 462 total aligned reads displayed.

AGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCNGACGGGCCCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGCGCA  >  NC_008463/1239010‑1239304

aGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGc                                                                                                                                                   <  1:5885722/150‑1 (MQ=255)

aGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCGATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGc                                                                                                                                                   >  2:2028911/1‑150 (MQ=255)

 gcgcTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGcc                                                                                                                                                  <  2:5028090/150‑1 (MQ=255)

   gcTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGcccc                                                                                                                                                <  2:3784057/150‑1 (MQ=255)

   gcTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGcccc                                                                                                                                                >  1:2540145/1‑150 (MQ=255)

      gAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGccccgtc                                                                                                                                             <  1:4778409/150‑4 (MQ=255)

         cGAGCGTTCGCCTAGACGGTCTATCTGGCTCTTGGGTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCaga                                                                                                                                         <  1:3071515/150‑1 (MQ=255)

          gAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCagag                                                                                                                                        >  2:3352108/1‑150 (MQ=255)

          gAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCagag                                                                                                                                        >  1:6678110/1‑150 (MQ=255)

              gTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTg                                                                                                                                    >  1:5234525/1‑150 (MQ=255)

               ttCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTgg                                                                                                                                   >  2:3660891/1‑150 (MQ=255)

               ttCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTgg                                                                                                                                   <  1:1600008/150‑1 (MQ=255)

               ttCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTgg                                                                                                                                   >  1:10959200/1‑150 (MQ=255)

                tCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAAGGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGc                                                                                                                                  <  2:1769223/150‑1 (MQ=255)

                     tCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAAccc                                                                                                                             <  1:11123806/150‑1 (MQ=255)

                      cGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCa                                                                                                                            <  1:7453060/150‑1 (MQ=255)

                        aCGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAgg                                                                                                                          >  2:8056732/1‑150 (MQ=255)

                         cGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGa                                                                                                                         <  1:3209334/150‑1 (MQ=255)

                         cGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGa                                                                                                                         <  1:3539328/150‑1 (MQ=255)

                          ggTCTATCGGGCTCTTTGGTCGGCCTTCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAAAGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTTGCAACCCAGGAg                                                                                                                        <  2:4320944/150‑1 (MQ=255)

                           gTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTTACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAgt                                                                                                                       <  1:7320563/150‑1 (MQ=255)

                             cTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAgtgt                                                                                                                     >  1:5649284/1‑150 (MQ=255)

                               aTCGGGCTCTTGGTTCGGCCTGCGCAGTGCCAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTACTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTcc                                                                                                                   <  2:2381576/150‑1 (MQ=255)

                                 cGGGCTCTAGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCgg                                                                                                                 <  2:1429429/150‑1 (MQ=255)

                                   ggCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGAt                                                                                                               >  2:1161237/1‑150 (MQ=255)

                                    gCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGAtt                                                                                                              <  1:3337928/150‑1 (MQ=255)

                                    gCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGAtt                                                                                                              >  1:526521/1‑150 (MQ=255)

                                        ttGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATc                                                                                                          <  2:1098369/150‑1 (MQ=255)

                                          ggTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATTATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGt                                                                                                        <  1:7003531/150‑1 (MQ=255)

                                          ggTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGt                                                                                                        <  1:10802892/150‑1 (MQ=255)

                                              cGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTc                                                                                                    <  2:9899517/150‑1 (MQ=255)

                                               ggCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTcc                                                                                                   >  2:1072124/1‑150 (MQ=255)

                                                gCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCACCCCAGGAGTGTCCGGAGTGAGCGTTTTCCg                                                                                                  >  2:9532347/1‑150 (MQ=255)

                                                gCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCg                                                                                                  >  1:2905822/1‑150 (MQ=255)

                                                gCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCg                                                                                                  <  1:9124274/150‑1 (MQ=255)

                                                 ccTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGt                                                                                                 <  2:5227526/150‑1 (MQ=255)

                                                  cTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGtt                                                                                                <  2:7537765/150‑1 (MQ=255)

                                                   tGCGCAGTGACAGGCCCGCCGACTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTa                                                                                               <  2:4365869/150‑1 (MQ=255)

                                                     cgcAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTAtt                                                                                             <  2:2132772/150‑1 (MQ=255)

                                                       cAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTg                                                                                           >  1:4639603/1‑150 (MQ=255)

                                                        aGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGt                                                                                          <  2:252128/150‑1 (MQ=255)

                                                          tGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTcc                                                                                        <  2:2711617/150‑1 (MQ=255)

                                                           gACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTccc                                                                                       >  2:9032861/1‑150 (MQ=255)

                                                               ggCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATGGATCGTTTTCCGTTATTTGTCCCGTGa                                                                                   >  2:7339599/1‑150 (MQ=255)

                                                                gCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGaa                                                                                  <  1:219325/150‑1 (MQ=255)

                                                                  ccgccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGt                                                                                >  1:10495034/1‑150 (MQ=255)

                                                                   cgccgAATCGCAGGATAATTCCGTCTATTTACTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTg                                                                               <  1:947703/150‑1 (MQ=255)

                                                                     ccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCt                                                                             <  2:5347305/150‑1 (MQ=255)

                                                                     ccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCt                                                                             <  1:9042898/150‑1 (MQ=255)

                                                                       gAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTgg                                                                           >  2:10430812/1‑150 (MQ=255)

                                                                       gAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTgg                                                                           <  1:7786719/150‑1 (MQ=255)

                                                                        aaTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGt                                                                          >  2:3178203/1‑150 (MQ=255)

                                                                        aaTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTGATTTGTCCCGTGAAGTGCTGGt                                                                          >  1:2784694/1‑150 (MQ=255)

                                                                          tCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCa                                                                        <  1:3808993/150‑1 (MQ=255)

                                                                           cGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCAc                                                                       <  1:1478581/150‑1 (MQ=255)

                                                                           cGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCAc                                                                       >  1:6248661/1‑150 (MQ=255)

                                                                            gCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGACACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGAGTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCAcc                                                                      >  2:5901167/1‑150 (MQ=255)

                                                                            gCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCAcc                                                                      <  2:6651802/150‑1 (MQ=255)

                                                                             cAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCa                                                                     >  2:1503190/1‑150 (MQ=255)

                                                                               ggATACTGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTGTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATg                                                                   >  1:8761471/1‑150 (MQ=255)

                                                                               ggATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATg                                                                   >  1:7599716/1‑150 (MQ=255)

                                                                               ggATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGGGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTACCGTGAAGTGCTGGTCACCATg                                                                   >  1:6497000/1‑150 (MQ=255)

                                                                                gATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGa                                                                  <  2:2992555/150‑1 (MQ=255)

                                                                                   aaTGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAgcg                                                               >  2:4987048/1‑150 (MQ=255)

                                                                                     tGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGcc                                                             >  2:6382379/1‑150 (MQ=255)

                                                                                         ggCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTGTCCGGTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCa                                                         >  2:40158/1‑150 (MQ=255)

                                                                                          gCTATTTTCTCCGCGCCCTGCCGCCATGGCCGGAAACGCGTGATGCCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTAATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAg                                                        <  2:8510653/150‑1 (MQ=255)

                                                                                          gCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAg                                                        <  1:1811088/150‑1 (MQ=255)

                                                                                          gCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAg                                                        >  2:776568/1‑150 (MQ=255)

                                                                                               tttCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCg                                                   >  1:4129726/1‑150 (MQ=255)

                                                                                               tttCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCg                                                   >  2:8779639/1‑150 (MQ=255)

                                                                                                ttCTCCGCGCCATGCCGCCAGGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGCTCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGt                                                  <  2:7813008/150‑1 (MQ=255)

                                                                                                 tctcCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtg                                                 <  2:6290740/150‑1 (MQ=255)

                                                                                                 tctcCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtg                                                 >  2:5627449/1‑150 (MQ=255)

                                                                                                  ctcCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtgc                                                <  1:3670259/150‑1 (MQ=255)

                                                                                                     cgcgCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtgctgc                                             >  2:4511713/1‑150 (MQ=255)

                                                                                                      gcgcCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTgctgct                                            >  1:5043629/1‑150 (MQ=255)

                                                                                                        gcCATGCCGCCATGGCCGGACACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTAGTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTgg                                          >  2:3130228/1‑150 (MQ=255)

                                                                                                         ccATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGt                                         >  1:849523/1‑150 (MQ=255)

                                                                                                           aTGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGa                                       <  1:10152292/150‑1 (MQ=255)

                                                                                                            tGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGat                                      <  2:9298812/150‑1 (MQ=255)

                                                                                                            tGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGat                                      >  2:6920780/1‑150 (MQ=255)

                                                                                                            tGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGat                                      >  1:2109651/1‑150 (MQ=255)

                                                                                                              ccgccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGatca                                    >  2:543761/1‑150 (MQ=255)

                                                                                                                 ccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGAtcatca                                 >  2:1922260/1‑150 (MQ=255)

                                                                                                                 ccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGAtcatca                                 >  2:831942/1‑150 (MQ=255)

                                                                                                                 ccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGAtcatca                                 >  1:2635600/1‑150 (MQ=255)

                                                                                                                  cATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATcatcat                                >  1:4519396/1‑150 (MQ=255)

                                                                                                                     ggCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAg                             <  1:122256/150‑1 (MQ=255)

                                                                                                                      gCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGt                            >  1:1089364/1‑150 (MQ=255)

                                                                                                                         ggAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGc                         >  2:6969451/1‑150 (MQ=255)

                                                                                                                                cgTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTtca                  >  1:825344/1‑150 (MQ=255)

                                                                                                                                  tgatgaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTtcatc                <  2:9867082/150‑1 (MQ=255)

                                                                                                                                      gaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGt            <  1:5401749/150‑1 (MQ=255)

                                                                                                                                       aCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTa           <  2:10526575/150‑1 (MQ=255)

                                                                                                                                        cGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTAc          <  1:2316983/150‑1 (MQ=255)

                                                                                                                                         ggggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTAcc         >  1:5778915/1‑150 (MQ=255)

                                                                                                                                            gCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTgg      <  2:8630913/150‑1 (MQ=255)

                                                                                                                                              cgACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGgcg    >  1:6079433/2‑150 (MQ=255)

                                                                                                                                               gACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTGTCCTGTATTTGTCCCGTGAAGTGCTGGTCACCCTGAGCGCAGTCAGCGCCGTGCTTCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGgcgc   <  1:994000/150‑1 (MQ=255)

AGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCNGACGGGCCCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGCGCA  >  NC_008463/1239010‑1239304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`