Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463934,2470AG40.6% 518.5 / inf 475Y92C (TAC→TGC) ampRLysR family transcriptional regulator AmpR
Reads supporting (aligned to +/- strand):  ref base A (149/133);  new base G (95/98);  total (244/231)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 483 total aligned reads displayed.

GAGCCTCGAGGAGCGTCTCGGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATCGCCGCCGAGGGGCTCG  >  NC_008463/934098‑934396
                                                                                                                                                     |                                                                                                                                                     
  gCCTCGAGGAGCGTCTCGGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTAcc                                                                                                                                                     <  2:4040009/150‑1 (MQ=255)
     tCGAGGAGCGTCTCGGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCggg                                                                                                                                                  <  2:10578579/150‑1 (MQ=255)
     tCGAGGAGCGTCTCGGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCggg                                                                                                                                                  >  1:3438217/1‑150 (MQ=255)
      cGAGGAGCGTCTCGGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGa                                                                                                                                                 >  1:240754/1‑150 (MQ=255)
      cGAGGAGCGTCTCGGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGa                                                                                                                                                 >  2:5234131/1‑150 (MQ=255)
         ggagCGTCTCGGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGt                                                                                                                                              <  2:10758583/150‑1 (MQ=255)
              gTCTCGGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCa                                                                                                                                         <  1:6619093/150‑1 (MQ=255)
                 tcGGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCg                                                                                                                                      >  1:5956338/1‑150 (MQ=255)
                 tcGGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCg                                                                                                                                      <  2:4366048/150‑1 (MQ=255)
                  cGGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGt                                                                                                                                     <  1:2671611/150‑1 (MQ=255)
                   ggCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTc                                                                                                                                    <  1:2221550/150‑1 (MQ=255)
                       tGGCCCTGTACAAGCGTCTGCCGCGCGGCCTGAGGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGgcg                                                                                                                                <  2:7746194/150‑1 (MQ=255)
                            cTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTc                                                                                                                           >  1:8798038/1‑150 (MQ=255)
                             tggtCAAGCGTCTGCCGCGCGGCCTGCAGCTGACCCCCGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCAGCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCg                                                                                                                          <  2:6237347/147‑1 (MQ=255)
                              gTTCAAGCGTCTGCCGCGCGGCCTGATGCGGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCgg                                                                                                                         <  2:3289919/150‑1 (MQ=255)
                               ttCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGa                                                                                                                        >  1:4736263/1‑150 (MQ=255)
                                  aaGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAAcc                                                                                                                     <  2:4502256/150‑1 (MQ=255)
                                    gCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCtt                                                                                                                   >  1:8468985/1‑150 (MQ=255)
                                      gTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCa                                                                                                                 <  2:783899/150‑1 (MQ=255)
                                       tCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCAc                                                                                                                >  2:10321447/1‑150 (MQ=255)
                                        cTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACg                                                                                                               <  2:5538527/150‑1 (MQ=255)
                                        cTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACg                                                                                                               <  1:1270263/150‑1 (MQ=255)
                                        cTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACg                                                                                                               <  2:2588610/150‑1 (MQ=255)
                                         tGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACAACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACgg                                                                                                              >  2:6497418/1‑150 (MQ=255)
                                            cgcgcgGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCg                                                                                                           >  1:3944390/1‑150 (MQ=255)
                                            cgcgcgGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCg                                                                                                           >  2:2818818/1‑150 (MQ=255)
                                              cgcgGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCggc                                                                                                         <  1:4479169/150‑1 (MQ=255)
                                                 ggCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCggctgg                                                                                                      <  1:7389498/150‑1 (MQ=255)
                                                 ggCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCggctgg                                                                                                      >  1:10505611/1‑150 (MQ=255)
                                                  gCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCggctggc                                                                                                     <  2:584323/150‑1 (MQ=255)
                                                     tGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGgctgc                                                                                                  >  1:4892268/1‑150 (MQ=255)
                                                     tGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGgctgc                                                                                                  >  1:8555031/1‑150 (MQ=255)
                                                     tGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGgctgc                                                                                                  >  2:9873111/1‑150 (MQ=255)
                                                             aCCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCgg                                                                                          >  1:8043768/1‑150 (MQ=255)
                                                             aCCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCgg                                                                                          >  1:9975004/1‑150 (MQ=255)
                                                              cccACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGc                                                                                         >  1:198571/1‑150 (MQ=255)
                                                               ccACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCt                                                                                        <  1:8099998/150‑1 (MQ=255)
                                                                  cGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGc                                                                                     <  1:6078347/150‑1 (MQ=255)
                                                                   gAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCg                                                                                    >  1:526547/1‑150 (MQ=255)
                                                                     gggCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGa                                                                                  >  1:5108875/1‑150 (MQ=255)
                                                                     gggCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGa                                                                                  >  2:2219925/1‑150 (MQ=255)
                                                                         gagaGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTc                                                                              <  1:10240366/150‑1 (MQ=255)
                                                                          agagCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTcc                                                                             >  1:7351560/1‑150 (MQ=255)
                                                                           gagCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCa                                                                            <  1:11017246/150‑1 (MQ=255)
                                                                           gagCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCa                                                                            <  2:8024484/150‑1 (MQ=255)
                                                                             gCCTGCTGCCGGTGCTGTGTGACTCCTTCTACAGCATCGCCGGCCTGCTGGATCGCTTCGAGGGCGGCCACTACCGTGAAGTGCTCACCGTCGGCGCGGTCGGAACCTTCACTGTCGGGTGGCTGCTGCCGCGGCTGGCGGACTTCCAgg                                                                          <  2:7523632/150‑1 (MQ=255)
                                                                                   tgcCGGTGCTGTGTGACTCCTTCGACCGCCTCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGcc                                                                    <  2:485495/150‑1 (MQ=255)
                                                                                       ggTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATcc                                                                >  1:5452441/1‑150 (MQ=255)
                                                                                         tGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCt                                                              >  1:6839368/1‑150 (MQ=255)
                                                                                          gCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCtt                                                             <  2:7223394/150‑1 (MQ=255)
                                                                                          gCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCtt                                                             <  2:1087611/150‑1 (MQ=255)
                                                                                          gCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCtt                                                             <  2:9066190/150‑1 (MQ=255)
                                                                                          gCTGTGTGACTCCTTCGACCGCAACGCCGGCCTGCTGGACCGCTTGGAGGGCGGCCACTACCGGGACGTGCTCACAGACGGCGCGGTCGGAACCTGCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTGCCAGGCGCGCCCTCCCtt                                                             >  2:659587/1‑150 (MQ=255)
                                                                                           cTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTc                                                            <  2:9642371/150‑1 (MQ=255)
                                                                                           cTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTc                                                            >  2:1380748/1‑150 (MQ=255)
                                                                                                tgACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGa                                                       <  2:122384/150‑1 (MQ=255)
                                                                                                 gACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGAt                                                      <  2:9277702/150‑1 (MQ=255)
                                                                                                   cTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCt                                                    <  1:4322650/150‑1 (MQ=255)
                                                                                                    tCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTg                                                   >  2:10551915/1‑150 (MQ=255)
                                                                                                    tCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTg                                                   <  2:5112242/150‑1 (MQ=255)
                                                                                                    tCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTg                                                   <  1:10142301/150‑1 (MQ=255)
                                                                                                     ccTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTgc                                                  >  2:7429944/1‑150 (MQ=255)
                                                                                                     ccTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTgc                                                  >  1:184559/1‑150 (MQ=255)
                                                                                                     cattCGACCGCATCGCAGGCCTGCTGTAACTCTACGAGGTCTGCAACTACCGGGACGTGATCACCGACGGAGCGGTCGGACCCAACACGGTCGGCTCGCAACTGCCGCGCATGGCCTACTTCCAGGCGCGCCATCCCTTCAACGATCtcc                                                  >  2:5387354/3‑148 (MQ=255)
                                                                                                         cGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCt                                              <  1:10766681/150‑1 (MQ=255)
                                                                                                         cGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCt                                              <  1:2509318/150‑1 (MQ=255)
                                                                                                         cGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCt                                              >  2:1172957/1‑150 (MQ=255)
                                                                                                         cGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCt                                              >  2:9387106/1‑150 (MQ=255)
                                                                                                          gACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTg                                             <  2:2130114/150‑1 (MQ=255)
                                                                                                          gACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTg                                             >  1:9408609/1‑150 (MQ=255)
                                                                                                           aCCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGt                                            >  1:6465828/1‑150 (MQ=255)
                                                                                                           aCCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGt                                            <  1:3274515/150‑1 (MQ=255)
                                                                                                                   gCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTccacccac                                    >  1:793412/1‑150 (MQ=255)
                                                                                                                      ggCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCacaac                                 <  2:6788310/150‑1 (MQ=255)
                                                                                                                      ggCCTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCacaac                                 <  1:5526495/150‑1 (MQ=255)
                                                                                                                      ggCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCacaac                                 >  2:1562230/1‑150 (MQ=255)
                                                                                                                        ccTGCTGGAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCAcaacaa                               <  2:247817/150‑1 (MQ=255)
                                                                                                                           gctgGATCGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACcg                            <  2:10158385/150‑1 (MQ=255)
                                                                                                                              ggAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGt                         <  1:9792502/150‑1 (MQ=255)
                                                                                                                              ggAACGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCGGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCTTCGATCTGCGCCTGTCCACCCACAACAACCGCGt                         >  2:195440/1‑150 (MQ=255)
                                                                                                                               gAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTc                        <  2:3936978/150‑1 (MQ=255)
                                                                                                                                  cGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCTCCGTCGGCGCGGTCGGAACCTTCACGGTCGGCGGGCTGCTGCCGCGGCTGGCGGACTTGCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGAc                     <  2:4249155/150‑1 (MQ=255)
                                                                                                                                  cGCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGTCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCCCCCACAACAACCGCGTCGAc                     <  2:9808326/150‑1 (MQ=255)
                                                                                                                                  cGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGAc                     >  2:8282465/1‑150 (MQ=255)
                                                                                                                                   gCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACa                    <  1:9564453/150‑1 (MQ=255)
                                                                                                                                   gCTTCGAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACa                    <  2:610447/150‑1 (MQ=255)
                                                                                                                                    cTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACAt                   <  1:2786727/150‑1 (MQ=255)
                                                                                                                                     ttCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATc                  <  1:9149945/150‑1 (MQ=255)
                                                                                                                                       cGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCCTCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATcgc                >  2:3739066/1‑150 (MQ=255)
                                                                                                                                        gAGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATcgcc               >  1:10868273/1‑150 (MQ=255)
                                                                                                                                         aGGGCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATcgccg              >  1:7650065/1‑150 (MQ=255)
                                                                                                                                         aGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATACCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATcgccg              >  1:3712619/1‑150 (MQ=255)
                                                                                                                                          gggCGGCCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATcgccgc             <  1:9039061/150‑1 (MQ=255)
                                                                                                                                              ggcCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATCGCCGCCGAg         <  1:3152020/150‑1 (MQ=255)
                                                                                                                                              ggcCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATCGCCGCCGAg         >  2:952681/1‑150 (MQ=255)
                                                                                                                                               gcCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATCGCCGCCGAgg        <  2:880189/150‑1 (MQ=255)
                                                                                                                                               gcCACTGCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATCGCCGCCGAgg        >  1:7018899/1‑150 (MQ=255)
                                                                                                                                                  aCTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACCACAACCGCGTCGACATCGCCGCCGAGGGGc     <  2:4659254/150‑1 (MQ=255)
                                                                                                                                                  aCTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATCGCCGCCGAGGGGc     >  1:7208978/1‑150 (MQ=255)
                                                                                                                                                     aCCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATCGCCGCCGAGGGGCTCg  <  1:1504946/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAGCCTCGAGGAGCGTCTCGGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATCGCCGCCGAGGGGCTCG  >  NC_008463/934098‑934396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑