Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,424,176 A→G T429A (ACC→GCC)  mexY → multidrug efflux RND transporter permease subunit MexY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,424,1760AG100.0% 2097.6 / NA 540T429A (ACC→GCC) mexYmultidrug efflux RND transporter permease subunit MexY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (278/262);  total (278/262)

Only 100 of 556 total aligned reads displayed.

ACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCCAAGAAGGCCTGTCGCCGCACGACACCACGGTCAAGGCGATGCGCCAAATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATC  >  NC_008463/3424029‑3424322
                                                                                                                                                   |                                                                                                                                                  
  ggTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGAcgccac                                                                                                                                                <  1:4892256/150‑1 (MQ=255)
  ggTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGAcgccac                                                                                                                                                <  2:2045986/150‑1 (MQ=255)
  ggTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCATGTCGCCGCACGAcgccac                                                                                                                                                <  2:3982725/150‑1 (MQ=255)
  ggTGATGCTCGGCCTGGGCTTCTCGAACAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGAAGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGAcgccac                                                                                                                                                <  2:8214122/150‑1 (MQ=21)
   gTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACg                                                                                                                                               >  1:9877373/1‑150 (MQ=255)
     gATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGt                                                                                                                                             <  2:8647671/150‑1 (MQ=255)
     gATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGt                                                                                                                                             >  1:6104445/1‑150 (MQ=255)
      aTGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTc                                                                                                                                            <  1:9278259/150‑1 (MQ=255)
        gCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCaa                                                                                                                                          <  2:6880560/150‑1 (MQ=255)
         cTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAg                                                                                                                                         <  1:6725852/150‑1 (MQ=255)
          tCGGCCTGGGCTTCTCGATCCACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAgg                                                                                                                                        <  2:7555037/150‑1 (MQ=255)
          tCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAgg                                                                                                                                        <  1:6928978/150‑1 (MQ=255)
            ggCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCg                                                                                                                                      >  2:4910438/1‑150 (MQ=255)
             gCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGa                                                                                                                                     >  2:9533055/1‑150 (MQ=255)
             gCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGa                                                                                                                                     <  1:8081935/150‑1 (MQ=255)
                tGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATgc                                                                                                                                  >  1:5427339/1‑150 (MQ=255)
                 gggCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATgcg                                                                                                                                 >  2:11019394/1‑150 (MQ=255)
                   gCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGcc                                                                                                                               <  1:2504721/150‑1 (MQ=255)
                     ttCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCag                                                                                                                             <  1:8680248/150‑2 (MQ=255)
                      tctcGCTCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGAGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCACGGCGATGCGCCaga                                                                                                                            <  2:4329642/150‑3 (MQ=21)
                      tctcGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCaga                                                                                                                            >  1:8728430/1‑148 (MQ=255)
                         cGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCa                                                                                                                         >  2:5885435/1‑150 (MQ=255)
                          gATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTTGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAg                                                                                                                        >  1:10085474/1‑150 (MQ=21)
                          gATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAg                                                                                                                        >  1:11044513/1‑150 (MQ=255)
                          gATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAg                                                                                                                        >  1:7348249/1‑150 (MQ=255)
                          gATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAg                                                                                                                        <  1:283750/150‑1 (MQ=255)
                           aTCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGc                                                                                                                       >  1:2160325/1‑150 (MQ=255)
                                      gACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgt                                                                                                            <  2:7022652/150‑1 (MQ=255)
                                       aCCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgtc                                                                                                           <  1:4533251/150‑1 (MQ=255)
                                       aCCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgtc                                                                                                           >  2:10885762/1‑150 (MQ=255)
                                         cATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCgg                                                                                                         >  1:5509642/1‑150 (MQ=255)
                                                   aTGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtg                                                                                               >  1:96059/1‑150 (MQ=255)
                                                    tGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtgg                                                                                              <  1:4694392/150‑1 (MQ=255)
                                                    tGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtgg                                                                                              <  2:3188067/150‑1 (MQ=255)
                                                     ggTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtggt                                                                                             <  1:5666608/150‑1 (MQ=255)
                                                     ggTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtggt                                                                                             <  1:6964237/150‑1 (MQ=255)
                                                       tCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGc                                                                                           <  2:34311/150‑1 (MQ=255)
                                                        ccTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCt                                                                                          <  2:1885448/150‑1 (MQ=255)
                                                          tGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTgg                                                                                        <  1:3931040/150‑1 (MQ=255)
                                                          tGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTgg                                                                                        >  1:1748666/1‑150 (MQ=255)
                                                           ggCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGt                                                                                       >  2:8404372/1‑150 (MQ=255)
                                                            gCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGtc                                                                                      <  2:3015887/150‑1 (MQ=255)
                                                            gCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGtc                                                                                      <  2:4206417/150‑1 (MQ=255)
                                                             cGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGtct                                                                                     <  2:7710525/150‑1 (MQ=255)
                                                             cGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGtct                                                                                     <  2:1341059/150‑1 (MQ=255)
                                                             cGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGtct                                                                                     <  2:626412/150‑1 (MQ=255)
                                                                tCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCgg                                                                                  <  1:10600939/150‑1 (MQ=255)
                                                                  ggCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTa                                                                                >  2:3730687/1‑150 (MQ=255)
                                                                    cATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTAtt                                                                              <  2:9791311/150‑1 (MQ=255)
                                                                     aTCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCCAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGGGCTGGTCTCGGTAGTc                                                                             >  1:837460/1‑150 (MQ=25)
                                                                      tCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCg                                                                            >  1:1018307/1‑150 (MQ=255)
                                                                       ccTGGTGGACGACGCGATCATCGTGGTGGAGATCGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGt                                                                           <  2:2745419/150‑1 (MQ=38)
                                                                         tggtggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGc                                                                         <  1:5908928/150‑1 (MQ=255)
                                                                            tggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGa                                                                      >  2:10265265/1‑150 (MQ=255)
                                                                              gacgacGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATg                                                                    <  1:4023923/150‑1 (MQ=255)
                                                                               acgacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATgg                                                                   <  2:5568967/150‑1 (MQ=255)
                                                                               acgacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCACGGTATTCGTGCCGATgg                                                                   >  2:9407168/1‑150 (MQ=37)
                                                                                 gacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCg                                                                 <  2:507142/150‑1 (MQ=255)
                                                                                 gacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCg                                                                 >  2:4488998/1‑150 (MQ=255)
                                                                                  acgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGt                                                                >  1:5186350/1‑150 (MQ=255)
                                                                                    gcgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttc                                                              <  2:4653049/150‑1 (MQ=255)
                                                                                    gcgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttc                                                              >  2:427573/1‑150 (MQ=255)
                                                                                    gcgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttc                                                              <  2:2843713/150‑1 (MQ=255)
                                                                                      gATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttctt                                                            >  1:7593163/1‑150 (MQ=255)
                                                                                       atcatcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttcttc                                                           >  1:7720594/1‑150 (MQ=255)
                                                                                         catcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAg                                                         >  2:10706641/1‑150 (MQ=255)
                                                                                          atcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgc                                                        <  2:5412794/150‑1 (MQ=255)
                                                                                            cGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCTGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGTCGTTCTTCAgcgg                                                      <  2:3098444/150‑1 (MQ=37)
                                                                                             gtggtgGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcggc                                                     <  2:4819996/150‑1 (MQ=255)
                                                                                               ggtggAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGGTCTTCAGCGgcgc                                                   >  1:10893312/1‑150 (MQ=38)
                                                                                                gtggAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGcgcg                                                  >  2:7209939/1‑150 (MQ=255)
                                                                                                 tggAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCgg                                                 <  1:5032357/150‑1 (MQ=255)
                                                                                                    agaACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTTTTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTgg                                              <  1:11131523/150‑1 (MQ=38)
                                                                                                        cGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCaa                                          <  2:6668948/150‑1 (MQ=255)
                                                                                                         gTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAAc                                         >  2:9159482/1‑150 (MQ=255)
                                                                                                          tCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACa                                        >  1:7159750/1‑150 (MQ=255)
                                                                                                          tCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACa                                        <  1:3569634/150‑1 (MQ=255)
                                                                                                              gCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCTAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTTGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGAGGTGGGCAACATCTa                                    <  2:888322/150‑1 (MQ=38)
                                                                                                                ggCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTAcc                                  <  1:2988964/150‑1 (MQ=255)
                                                                                                                 gCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCg                                 >  1:8882392/1‑150 (MQ=255)
                                                                                                                  cTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGc                                <  1:10355884/150‑1 (MQ=255)
                                                                                                                  cTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGc                                >  1:6299160/1‑150 (MQ=255)
                                                                                                                  cTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGc                                >  1:7854333/1‑150 (MQ=255)
                                                                                                                   tGATGTCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGAGCCAGAACAGCGGGGCCAGCGTCGGCATCACCGAGGTGCTGGTCTCGGTATTCGTGCCGAAGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGcc                               >  1:9426901/1‑150 (MQ=255)
                                                                                                                     aTGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAg                             <  1:9768177/150‑1 (MQ=255)
                                                                                                                     aTGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAg                             >  2:1567566/1‑150 (MQ=255)
                                                                                                                      tGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGt                            <  2:5288523/150‑1 (MQ=255)
                                                                                                                      tGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGt                            >  2:7363277/1‑150 (MQ=255)
                                                                                                                        gccgtggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCCTCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTc                          >  1:10715846/7‑150 (MQ=255)
                                                                                                                        gccgaggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTc                          <  2:10138791/144‑1 (MQ=255)
                                                                                                                         ccgaggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTcg                         <  1:4363174/145‑1 (MQ=255)
                                                                                                                              gaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTg                    <  1:1929942/150‑1 (MQ=255)
                                                                                                                              gaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTg                    <  1:6569807/150‑1 (MQ=255)
                                                                                                                                      gTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGc            >  2:7838278/1‑150 (MQ=255)
                                                                                                                                        cgccgcACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCgg          >  2:10538713/1‑150 (MQ=255)
                                                                                                                                            gcACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGtctc      >  1:1533426/1‑150 (MQ=255)
                                                                                                                                            gcACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGtctc      >  1:2582461/1‑150 (MQ=255)
                                                                                                                                             cACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTcc     >  1:3323230/1‑150 (MQ=255)
                                                                                                                                              acgacgCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCa    >  2:2324176/1‑150 (MQ=255)
                                                                                                                                                gACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATc  >  1:8262090/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCCAAGAAGGCCTGTCGCCGCACGACACCACGGTCAAGGCGATGCGCCAAATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATC  >  NC_008463/3424029‑3424322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑