Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,879,553 A→G F52L (TTC→CTC)  PA14_RS17695 ← LysE family translocator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,879,5530AG99.8% 1581.1 / NA 414F52L (TTC→CTC) PA14_RS17695LysE family translocator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (217/196);  minor base T (0/1);  total (217/197)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 418 total aligned reads displayed.

CGTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAAAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAATT  >  NC_008463/3879406‑3879699
                                                                                                                                                   |                                                                                                                                                  
cGTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGagaa                                                                                                                                                  <  2:7012947/150‑1 (MQ=255)
 gTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGCCGCCGAGCCAGGCCAGGTCCAGCGCGCCGAGCGCCTGCAGGATGGCGCACAGCACAGGCGTACGGCTGAGCAGCAGCGACCCGCCGGTCAGCACCAGGGGGGTATGCCGGAGAAt                                                                                                                                                 >  2:3842134/1‑150 (MQ=255)
  tCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATc                                                                                                                                                <  2:10804042/150‑1 (MQ=255)
   cgccgcgccgCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATcc                                                                                                                                               >  1:2780029/1‑150 (MQ=255)
    gccgcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATccc                                                                                                                                              >  2:8174684/1‑150 (MQ=255)
       gcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCa                                                                                                                                           <  2:11077000/150‑1 (MQ=255)
        cgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAg                                                                                                                                          <  1:8679106/150‑1 (MQ=255)
         gccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAgg                                                                                                                                         <  1:1787300/150‑1 (MQ=255)
          ccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGc                                                                                                                                        >  1:7506478/1‑150 (MQ=255)
            gcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCa                                                                                                                                      <  2:7787082/150‑1 (MQ=255)
               gCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGcc                                                                                                                                   <  2:4008179/150‑1 (MQ=255)
                     cgcgcAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAgcg                                                                                                                             <  1:4185074/150‑1 (MQ=255)
                           gcgcgcCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTgg                                                                                                                       >  1:9628884/1‑150 (MQ=255)
                               gccgacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAg                                                                                                                   <  1:1430389/150‑1 (MQ=255)
                                ccgacgccgaGCCAGGCCAGGTACAGCTCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTCCGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTAGGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAgg                                                                                                                  <  2:4835824/150‑1 (MQ=255)
                                ccgacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAgg                                                                                                                  >  1:9725243/1‑150 (MQ=255)
                                ccgacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAgg                                                                                                                  <  1:4379870/150‑1 (MQ=255)
                                 cgccgccgaGCCAGGCCAGGTACCGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAggc                                                                                                                 <  2:4495599/147‑1 (MQ=255)
                                       cgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGccc                                                                                                           <  2:9506279/150‑1 (MQ=255)
                                       cgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGccc                                                                                                           >  1:10006421/1‑150 (MQ=255)
                                        gaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCccg                                                                                                          >  2:3538988/1‑150 (MQ=255)
                                        gaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCccg                                                                                                          >  2:10474242/1‑150 (MQ=255)
                                             aggccaggTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCgccgcc                                                                                                     >  1:4908417/1‑150 (MQ=255)
                                             aggccaggTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCgccgcc                                                                                                     >  1:229260/1‑150 (MQ=255)
                                             aggccaggTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCGGGCCAGGCCGGCCCgccgcc                                                                                                     <  1:4661660/150‑1 (MQ=255)
                                               gccaggTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGcccc                                                                                                   >  2:3261757/1‑150 (MQ=255)
                                               gccaggTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGcccc                                                                                                   <  1:18614/150‑1 (MQ=255)
                                                 caggTACAGCGCGCCGAGCGCCTGCAGGATGGCGTACAGCACAGGCGTCCGGCTGAGCAGCAGCGACACGCCGGTCCGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCGCCCGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTg                                                                                                 >  2:9258809/1‑150 (MQ=255)
                                                   ggTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGt                                                                                               >  2:10285191/1‑150 (MQ=255)
                                                         gcgcgcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAggg                                                                                         >  1:10949690/1‑150 (MQ=255)
                                                          cgcgcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTTCGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGa                                                                                        <  2:5811491/150‑1 (MQ=255)
                                                          cgcgcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGa                                                                                        <  1:7326660/150‑1 (MQ=255)
                                                          cgcgcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGa                                                                                        <  2:3320767/150‑1 (MQ=255)
                                                           gcgcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGAc                                                                                       >  2:1242514/1‑150 (MQ=255)
                                                            cgcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGCCg                                                                                      >  2:2014397/1‑150 (MQ=255)
                                                             gcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGt                                                                                     <  2:2530965/150‑1 (MQ=255)
                                                              ccGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTg                                                                                    <  1:2185471/150‑1 (MQ=255)
                                                                  gcgcCTGCAGGATGGCGATCAGCACAGGCGTACGGCTGAGCAGCAGAGACACGCCGGTCAGCTCCAGGGTGGTATGCAGGAGATTCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCTCCCCTGGTGCAGGGACGTGCGca                                                                                <  2:10925434/150‑1 (MQ=255)
                                                                   cgcCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGcac                                                                               >  2:5025470/1‑150 (MQ=255)
                                                                   cgcCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGcac                                                                               >  1:392083/1‑150 (MQ=255)
                                                                      cTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGcaccac                                                                            <  1:8511377/150‑1 (MQ=255)
                                                                      cTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGcaccac                                                                            <  1:2952374/150‑1 (MQ=255)
                                                                       tGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCaccacc                                                                           >  1:5345375/1‑150 (MQ=255)
                                                                       tGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCaccacc                                                                           <  1:1552410/150‑1 (MQ=255)
                                                                        gCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCAccacca                                                                          >  1:3325218/1‑150 (MQ=255)
                                                                          aGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGACTCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAgg                                                                        <  2:1620324/150‑1 (MQ=255)
                                                                           ggATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAggg                                                                       >  1:6974452/1‑150 (MQ=255)
                                                                            gATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGc                                                                      <  2:6638551/150‑1 (MQ=255)
                                                                            gATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGc                                                                      >  2:6460394/1‑150 (MQ=255)
                                                                                 cGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACag                                                                 >  1:10435370/1‑149 (MQ=255)
                                                                                   aaCAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGAc                                                                   >  2:4764007/1‑146 (MQ=255)
                                                                                    aCAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCgg                                                              >  2:895418/1‑150 (MQ=255)
                                                                                       gCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGAc                                                           <  2:6214866/150‑1 (MQ=255)
                                                                                       gCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGAc                                                           <  1:1980430/150‑1 (MQ=255)
                                                                                        cacaGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGAcc                                                          >  1:8016528/1‑150 (MQ=255)
                                                                                          caGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCgg                                                        >  2:2123489/1‑150 (MQ=255)
                                                                                             gCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGc                                                     >  1:6213988/1‑150 (MQ=255)
                                                                                             gCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGc                                                     <  1:8587783/150‑1 (MQ=255)
                                                                                                tACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGa                                                  >  2:8001439/1‑150 (MQ=255)
                                                                                                tACGGCTGAGCAGCAGCGACAAGCCGGTCAGCACCAGGGAGGTATGCAGGAGCCTCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGa                                                  <  2:5219360/150‑1 (MQ=255)
                                                                                                 aCGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGAc                                                 >  2:6265200/1‑150 (MQ=255)
                                                                                                   ggCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCa                                               >  1:4039199/1‑150 (MQ=255)
                                                                                                    gCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAg                                              >  1:7754219/1‑150 (MQ=255)
                                                                                                    gCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAg                                              <  2:5894081/150‑1 (MQ=255)
                                                                                                     cTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGCCCGGGGCTGACCAgg                                             >  1:8082899/1‑150 (MQ=255)
                                                                                                      tGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAggg                                            <  2:865497/150‑1 (MQ=255)
                                                                                                       gAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGc                                           >  2:9735131/1‑150 (MQ=255)
                                                                                                        agcagcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCg                                          >  1:10156710/1‑150 (MQ=255)
                                                                                                        agcagcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCg                                          >  2:2735460/1‑150 (MQ=255)
                                                                                                            gcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCTCCGCCCCTGGTGCAGGGACGTGCGCACAACCAGGGCGACATCGGGACCTGGGCTGACCAGGGCGACgg                                      >  2:2897189/1‑150 (MQ=255)
                                                                                                            gcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACgg                                      <  1:4761491/150‑1 (MQ=255)
                                                                                                            gcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACgg                                      <  2:8725392/150‑1 (MQ=255)
                                                                                                            gcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACgg                                      >  2:9190920/1‑150 (MQ=255)
                                                                                                            gcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACgg                                      >  1:2582349/1‑150 (MQ=255)
                                                                                                              agcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCg                                    >  2:4261984/1‑150 (MQ=255)
                                                                                                               gcGTCACGCCGGTCAGCTCCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCGCCTCCAGGGCGACCGCGGGACCGGGGCGGACCAGGGCGGCGGCGa                                   >  2:6031355/1‑150 (MQ=255)
                                                                                                                  acacGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGGGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAaca                                >  2:5543290/1‑150 (MQ=255)
                                                                                                                   cacGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAacac                               <  1:5832727/150‑1 (MQ=255)
                                                                                                                    acGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACAcc                              <  2:2349831/150‑1 (MQ=255)
                                                                                                                      gCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCGGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCCGGGCGACGGCGAACAccgc                            >  2:7472221/1‑150 (MQ=255)
                                                                                                                         ggTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACcgccgc                         <  1:8926289/150‑1 (MQ=255)
                                                                                                                         ggTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACcgccgc                         >  1:6476591/1‑150 (MQ=255)
                                                                                                                          gTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCgccgcc                        <  1:1067829/150‑1 (MQ=255)
                                                                                                                           tCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCgccgcct                       >  1:7908690/1‑149 (MQ=255)
                                                                                                                              gCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAggg                    >  2:10128307/1‑150 (MQ=255)
                                                                                                                              gCACCAGGCTGGTATGCAGGAGAAACCCGCAGGCCAGCCCCCGCGCGCTGGCCCGGCCGGCCCGCCGCCCCTGGTGCAGGGACGGGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAggg                    <  2:1703571/150‑1 (MQ=255)
                                                                                                                               cACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCCGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGt                   >  2:1476502/1‑150 (MQ=255)
                                                                                                                                    gggTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCa              >  1:6677946/1‑150 (MQ=255)
                                                                                                                                        ggtATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagac          >  1:8861362/1‑150 (MQ=255)
                                                                                                                                         gtATGCAGTAGAATCGCGCAGGCCAGCCCCTGCGCGCTGGCCTGGCCGGCCCGCCGCCCATGGTGCAGGGACGTGCGGACCACCAGGGCTACATCGGGACGGGGGCTGACCTTTGCGACGGCGTACACAGCCGCCAGGGTCAGCAagaca         <  1:5560098/150‑1 (MQ=255)
                                                                                                                                          tATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacag        <  1:8328687/150‑1 (MQ=255)
                                                                                                                                           aTGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCCGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacaga       <  2:4376664/150‑1 (MQ=255)
                                                                                                                                           aTGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacaga       <  1:3542955/150‑1 (MQ=255)
                                                                                                                                           aTGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacaga       >  1:7654380/1‑150 (MQ=255)
                                                                                                                                            tGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGCCCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacagac      >  2:8402353/1‑150 (MQ=255)
                                                                                                                                              cAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACaa    <  1:9229273/150‑1 (MQ=255)
                                                                                                                                               aGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAt   >  2:8768011/1‑150 (MQ=255)
                                                                                                                                                ggAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAtt  <  1:514480/150‑1 (MQ=255)
                                                                                                                                                ggAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAtt  >  1:9867571/1‑150 (MQ=255)
                                                                                                                                                ggAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAtt  <  2:9830348/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAAAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAATT  >  NC_008463/3879406‑3879699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑