Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 982,940 A→G F293F (TTT→TTC)  PA14_RS04570 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463982,9400AG100.0% 1940.6 / NA 494F293F (TTT→TTC) PA14_RS04570ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (225/269);  total (225/269)

Only 100 of 496 total aligned reads displayed.

CTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACAAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAG  >  NC_008463/982793‑983086
                                                                                                                                                   |                                                                                                                                                  
 tCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAAc                                                                                                                                                 <  1:7345547/150‑1 (MQ=255)
 tCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAAc                                                                                                                                                 >  1:8536882/1‑150 (MQ=255)
 tCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAAc                                                                                                                                                 >  2:2892668/1‑150 (MQ=255)
  cGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACa                                                                                                                                                <  2:6627068/150‑1 (MQ=255)
   ggCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAg                                                                                                                                               <  2:2965282/150‑1 (MQ=255)
    gCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGt                                                                                                                                              <  1:10626037/150‑1 (MQ=255)
     ccAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTg                                                                                                                                             >  1:7590616/1‑150 (MQ=255)
     ccAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTg                                                                                                                                             <  1:4691314/150‑1 (MQ=255)
        gcagcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCt                                                                                                                                          <  2:7902139/150‑1 (MQ=255)
          agcagGCCGAAGATGTGCCAGGGGCGCGCGCCCCCCGAGCGCAGGGTGGCCATCTCCCGGCGGCGCTCGGTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGTTCAGGCCGGGGAGGACGACGAACCGCGAGTCCACGAACAGTGCCGtc                                                                                                                                        >  2:10801372/1‑150 (MQ=255)
          agcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtc                                                                                                                                        <  2:8355042/150‑1 (MQ=255)
           gcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGAGCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtct                                                                                                                                       >  1:6160726/1‑150 (MQ=255)
            cagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtctc                                                                                                                                      <  1:6902790/150‑1 (MQ=255)
            cagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtctc                                                                                                                                      <  2:4587330/150‑1 (MQ=255)
            cagGCCGAAGATGTGCCAGCGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtctc                                                                                                                                      <  2:10353091/150‑1 (MQ=255)
             agGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcg                                                                                                                                     <  2:8522982/150‑1 (MQ=255)
             agGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcg                                                                                                                                     >  2:4003102/1‑150 (MQ=255)
              ggCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcgg                                                                                                                                    <  1:8886907/150‑1 (MQ=255)
                      aTGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGccc                                                                                                                            >  2:9759845/1‑150 (MQ=255)
                      aTGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGccc                                                                                                                            <  1:2062913/150‑1 (MQ=255)
                       tgtgCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGAGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCACGGCGGTGCCCa                                                                                                                           >  2:1865711/1‑150 (MQ=255)
                       tgtgCCAGGGGCGCGCGCCCACCGAGCGCAGGAGGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCa                                                                                                                           <  2:10510090/150‑1 (MQ=255)
                         tgCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTGGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATc                                                                                                                         <  2:3682508/150‑1 (MQ=255)
                           ccAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCTAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAg                                                                                                                       <  2:3137951/150‑1 (MQ=255)
                             aGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGc                                                                                                                     >  1:726159/1‑150 (MQ=255)
                             aGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGc                                                                                                                     >  1:6320658/1‑150 (MQ=255)
                              ggggCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGcc                                                                                                                    >  2:5476290/1‑150 (MQ=255)
                                   gcgcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCcaca                                                                                                               >  2:815936/1‑150 (MQ=255)
                                    cgcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAg                                                                                                              <  1:10974148/150‑1 (MQ=255)
                                     gcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGt                                                                                                             <  1:160531/150‑1 (MQ=255)
                                        cccACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTcc                                                                                                          >  1:880694/1‑150 (MQ=255)
                                         ccACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCt                                                                                                         <  2:4151480/150‑1 (MQ=255)
                                          cACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTg                                                                                                        >  2:2228542/1‑150 (MQ=255)
                                            ccGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCGGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAAAAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCa                                                                                                      <  1:2594520/150‑1 (MQ=255)
                                              gAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAgg                                                                                                    >  1:9053988/1‑150 (MQ=255)
                                               aGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAggg                                                                                                   >  1:7717536/1‑150 (MQ=255)
                                               aGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAggg                                                                                                   <  1:8686604/150‑1 (MQ=255)
                                                gcgcAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGc                                                                                                  >  2:10972108/1‑150 (MQ=255)
                                                     ggATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGacac                                                                                             >  2:7349988/1‑150 (MQ=255)
                                                       aTGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCg                                                                                           <  2:7203074/150‑1 (MQ=255)
                                                       aTGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGGGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCg                                                                                           <  2:6967512/150‑1 (MQ=255)
                                                        tGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGGGAGGACGACGAACAGCGAGACCACGAACAGTGGCTTCTCGGCGGTGCCCATCAGGCCCCACAGGTCCTGCAGGGCGACACCgg                                                                                          >  1:8090584/1‑150 (MQ=255)
                                                           ccATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGgga                                                                                       >  1:2511787/1‑150 (MQ=255)
                                                            cATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGCTCGCCGTGAGCCTGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGggag                                                                                      <  2:6051950/150‑1 (MQ=255)
                                                              tctcCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGggagga                                                                                    >  1:288903/1‑150 (MQ=255)
                                                               ctcCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGAt                                                                                   <  2:10191501/150‑1 (MQ=255)
                                                                tcCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATc                                                                                  <  1:1789135/150‑1 (MQ=255)
                                                                    ggcggcGCTCGTTGAGGCTGGTGAGGATCGCCGTGCGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCa                                                                              <  2:3215675/150‑1 (MQ=255)
                                                                     gcggcgCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCAt                                                                             <  1:4579053/150‑1 (MQ=255)
                                                                      cggcgCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATc                                                                            >  2:3859524/1‑150 (MQ=255)
                                                                          gcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCg                                                                        >  1:8343834/1‑150 (MQ=255)
                                                                           cTCGTTGAGGCTGGTGAGGATCGCCGTGCGCATGCCGATCAGGCCGGTGAGGACGACGCACAGCGATACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCgg                                                                       <  2:763131/150‑1 (MQ=255)
                                                                           cTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCgg                                                                       >  1:8263097/1‑150 (MQ=255)
                                                                           cTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCgg                                                                       <  1:9205980/150‑1 (MQ=255)
                                                                           cTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCgg                                                                       >  1:10879493/1‑150 (MQ=255)
                                                                            tCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGt                                                                      >  2:5895607/1‑150 (MQ=255)
                                                                            tCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGt                                                                      >  1:10131483/1‑150 (MQ=255)
                                                                             cGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGtt                                                                     <  1:3670509/150‑1 (MQ=255)
                                                                              gTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTc                                                                    >  1:9335195/1‑150 (MQ=255)
                                                                               gtGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCg                                                                   >  1:2271974/2‑150 (MQ=255)
                                                                                   ggCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCgg                                                               >  2:2546191/1‑150 (MQ=255)
                                                                                     cTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGa                                                             >  1:7197720/1‑150 (MQ=255)
                                                                                      tGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGAt                                                            >  2:5699073/1‑150 (MQ=255)
                                                                                        gTGAGGATCGCCGTGAGAATGCCGATCAGGCCGGTGAGGAAGACTAACAGCGAGACCTCGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTACTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAg                                                          <  2:520116/150‑1 (MQ=255)
                                                                                           aGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCg                                                       >  1:4106913/1‑150 (MQ=255)
                                                                                              aTCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTg                                                    >  1:9563151/1‑150 (MQ=255)
                                                                                                  ccGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGACCAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCt                                                <  2:5293070/150‑1 (MQ=255)
                                                                                                  ccGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCt                                                <  2:7443679/150‑1 (MQ=255)
                                                                                                  ccGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCt                                                >  1:5556682/1‑150 (MQ=255)
                                                                                                    gTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTcg                                              >  1:4909046/1‑150 (MQ=255)
                                                                                                        gCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGtt                                          <  1:3523709/150‑1 (MQ=255)
                                                                                                        gCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGtt                                          >  1:1382260/1‑150 (MQ=255)
                                                                                                        gCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGtt                                          >  1:6747982/1‑150 (MQ=255)
                                                                                                           tGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCa                                       >  2:1426810/1‑150 (MQ=255)
                                                                                                            gCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAg                                      <  2:8952752/150‑1 (MQ=255)
                                                                                                               gATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGc                                   >  1:4800353/1‑150 (MQ=255)
                                                                                                               gATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGc                                   >  1:9735955/1‑150 (MQ=255)
                                                                                                                  cAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGaa                                <  1:1223249/150‑1 (MQ=255)
                                                                                                                   aGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAg                               >  2:9236006/1‑150 (MQ=255)
                                                                                                                    ggCCTGTGAGGACGACGAACAGCGAGACCTCGTACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATAAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAgg                              <  2:4803692/150‑1 (MQ=255)
                                                                                                                    ggCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAgg                              >  1:10239105/1‑150 (MQ=255)
                                                                                                                     gCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGt                             <  1:10700659/150‑1 (MQ=255)
                                                                                                                     gCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGt                             >  1:9884844/1‑150 (MQ=255)
                                                                                                                     gCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGt                             >  2:8689108/1‑150 (MQ=255)
                                                                                                                          tGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCa                        <  1:3127952/150‑1 (MQ=255)
                                                                                                                             ggACGACGACCAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCt                     <  2:4657088/150‑1 (MQ=255)
                                                                                                                             ggACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCt                     <  1:7828874/150‑1 (MQ=255)
                                                                                                                             ggACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCt                     >  1:10016555/1‑150 (MQ=255)
                                                                                                                               acgacgAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTg                   >  1:1680274/1‑150 (MQ=255)
                                                                                                                                    gaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCtt              <  1:5378443/150‑1 (MQ=255)
                                                                                                                                     aaCAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTc             >  2:4387129/1‑150 (MQ=255)
                                                                                                                                     aaCAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTc             <  1:11075983/150‑1 (MQ=255)
                                                                                                                                       cAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAg           >  1:3894171/1‑150 (MQ=255)
                                                                                                                                       cAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAg           >  2:956520/1‑150 (MQ=255)
                                                                                                                                         gCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGc         <  2:3546784/150‑1 (MQ=255)
                                                                                                                                          cGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGcc        >  2:4990801/1‑150 (MQ=255)
                                                                                                                                           gagaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACCGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATCGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCGTGCTCTTCAGGCCg       >  2:1191320/1‑150 (MQ=255)
                                                                                                                                           gagaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCg       <  1:588063/150‑1 (MQ=255)
                                                                                                                                            agaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGa      >  2:1686877/1‑150 (MQ=255)
                                                                                                                                               ccACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCa   <  2:2457700/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACAAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAG  >  NC_008463/982793‑983086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑