Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,356,548 G→C R69G (CGT→GGT)  PA14_RS06375 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,356,5480GC99.8% 2025.3 / NA 523R69G (CGT→GGT) PA14_RS06375MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base C (206/315);  minor base A (1/0);  total (208/315)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 523 total aligned reads displayed.

TGCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACGGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGACCC  >  NC_008463/1356401‑1356694
                                                                                                                                                   |                                                                                                                                                  
tGCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGa                                                                                                                                                  >  2:6053121/1‑150 (MQ=255)
     tCGTAGCCGTGGAGGACGCCGATCAGGGTGGTGGATACCCCCAACAGCAGCAGCGAGGCGACCAGGCTTGACTTGCGCCCGATGCGGTCGCAGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGAGAGGACGGCGATACCGAAGGTg                                                                                                                                             >  2:3926517/1‑150 (MQ=255)
     tCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTg                                                                                                                                             >  1:5220232/1‑150 (MQ=255)
      cGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGa                                                                                                                                            <  1:8657543/150‑1 (MQ=255)
      cGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGa                                                                                                                                            <  1:1780603/150‑1 (MQ=255)
        tAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAgg                                                                                                                                          >  1:10893932/1‑150 (MQ=255)
         aGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGa                                                                                                                                         <  2:2237151/150‑1 (MQ=255)
            cGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAgg                                                                                                                                      <  2:3378025/150‑1 (MQ=255)
             ggggAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGgc                                                                                                                                     >  1:5491310/1‑150 (MQ=255)
              gggAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGgcg                                                                                                                                    >  1:939648/1‑150 (MQ=255)
               ggaggaCGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGgcgc                                                                                                                                   >  1:3598989/1‑150 (MQ=255)
                gaggaCGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCt                                                                                                                                  >  2:1179699/1‑150 (MQ=255)
                gaggaCGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCt                                                                                                                                  <  2:1149669/150‑1 (MQ=255)
                 aggaCGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTg                                                                                                                                 >  1:10598629/1‑150 (MQ=255)
                     cGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCa                                                                                                                             <  1:3951002/150‑1 (MQ=255)
                      gCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCAt                                                                                                                            <  1:4905242/150‑1 (MQ=255)
                       ccGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATc                                                                                                                           <  1:9014648/150‑1 (MQ=255)
                          aTCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgc                                                                                                                        <  1:214598/150‑1 (MQ=255)
                          aTCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgc                                                                                                                        >  1:10724278/1‑150 (MQ=255)
                           tCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgcg                                                                                                                       >  2:7266473/1‑150 (MQ=255)
                            cAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgcgc                                                                                                                      <  2:8896221/150‑1 (MQ=255)
                            cAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgcgc                                                                                                                      <  1:4327197/150‑1 (MQ=255)
                            cAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgcgc                                                                                                                      <  2:8670080/150‑1 (MQ=255)
                               ggtggtGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGt                                                                                                                   <  1:1152585/150‑1 (MQ=255)
                                 tggtggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGc                                                                                                                 <  1:2058238/150‑1 (MQ=255)
                                  ggtggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGcc                                                                                                                <  1:2550107/150‑1 (MQ=255)
                                   ggggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCg                                                                                                               <  2:2852763/148‑1 (MQ=255)
                                     ggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGa                                                                                                             <  1:8375898/150‑1 (MQ=255)
                                     ggATAACCCCAACAGCAGAAGCGAGGCGACAAGAGTAGACTTGCGCCCGAAGCGGTCTCCGAAGAGGCCGAACATCCCCGAGCCCCGCGGGCGACCGAGCAACGCGATACCGAACGTGAGGAAGGCGCTGAGCATCTGAGCGGTGCCGGa                                                                                                             >  2:4811904/1‑150 (MQ=255)
                                       aTACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATg                                                                                                           <  1:157755/150‑1 (MQ=255)
                                         aCCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTc                                                                                                         <  2:6844991/150‑1 (MQ=255)
                                          cccccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCt                                                                                                        <  2:7892781/150‑1 (MQ=255)
                                          cccccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCt                                                                                                        <  1:4141793/150‑1 (MQ=255)
                                          cccccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCt                                                                                                        <  2:947321/150‑1 (MQ=255)
                                           ccccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTg                                                                                                       <  1:1377172/150‑1 (MQ=255)
                                             ccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCg                                                                                                     <  2:7750834/150‑1 (MQ=255)
                                              cATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCgg                                                                                                    <  1:6641128/150‑1 (MQ=255)
                                               aTCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCggg                                                                                                   >  1:81821/1‑150 (MQ=255)
                                                 cagcagcagCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGgaa                                                                                                 <  1:5608474/150‑1 (MQ=255)
                                                  agcagcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGgaag                                                                                                >  1:5064975/1‑150 (MQ=255)
                                                   gcggcagcGAGGCGACCAGGGTTGACTTGCGGCCGATGCGGTCGCCGAAGGGGCCGTACAGCGCCGGGCCGCGCGGGCGAGCGGGGAAGGCGATGCGGGGGGGGGGGTCGGCGCGGGGCATCGGCGCGGTGCGGGGTGGCGGCGGgacga                                                                                               >  2:9173378/4‑150 (MQ=255)
                                                        agcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATAcc                                                                                          <  1:5910116/150‑1 (MQ=255)
                                                            aGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGAc                                                                                      >  1:2788073/1‑150 (MQ=255)
                                                            aGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGAc                                                                                      >  2:2033896/1‑150 (MQ=255)
                                                             ggCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGGGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGACGAATACCGGAcc                                                                                     >  1:9576664/1‑150 (MQ=255)
                                                                 aCCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATc                                                                                 >  1:3064834/1‑150 (MQ=255)
                                                                     gggTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCa                                                                             <  2:1707729/150‑1 (MQ=255)
                                                                       gTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAgg                                                                           <  2:7498197/150‑1 (MQ=255)
                                                                       gTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAgg                                                                           >  2:5347416/1‑150 (MQ=255)
                                                                       gTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAgg                                                                           <  2:6698732/150‑1 (MQ=255)
                                                                        ttGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAggg                                                                          <  2:2446010/150‑1 (MQ=255)
                                                                          gACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcg                                                                        >  1:7698833/1‑150 (MQ=255)
                                                                           aCTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcgg                                                                       >  2:5764515/1‑150 (MQ=255)
                                                                              tGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcggcgg                                                                    >  1:2490055/1‑150 (MQ=255)
                                                                              tGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcggcgg                                                                    <  2:2589994/150‑1 (MQ=255)
                                                                                cgcCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCa                                                                  <  2:7347224/150‑1 (MQ=255)
                                                                                  cccGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGt                                                                <  1:6501575/150‑1 (MQ=255)
                                                                                  cccGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGt                                                                <  1:2963001/150‑1 (MQ=255)
                                                                                    cGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTgg                                                              >  1:3697808/1‑150 (MQ=255)
                                                                                    cGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTgg                                                              <  1:7994953/150‑1 (MQ=255)
                                                                                    cGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTgg                                                              <  1:2867092/150‑1 (MQ=255)
                                                                                     gATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGc                                                             <  2:10596501/150‑1 (MQ=255)
                                                                                     gATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGc                                                             <  2:1068538/150‑1 (MQ=255)
                                                                                      aTGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCa                                                            <  2:3211546/150‑1 (MQ=255)
                                                                                      aTGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCa                                                            <  1:7113/150‑1 (MQ=255)
                                                                                         cGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAg                                                         <  2:1685559/150‑1 (MQ=255)
                                                                                          ggTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGa                                                        >  1:10009277/1‑150 (MQ=255)
                                                                                           gTCGCCGCAGAGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGGCGGATGTCTGCGGGAAGTATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGAc                                                       <  2:3630942/150‑1 (MQ=255)
                                                                                           gTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCCTAGAc                                                       >  2:5574354/1‑150 (MQ=255)
                                                                                           gTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGAc                                                       <  2:309597/150‑1 (MQ=255)
                                                                                              gCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTa                                                    >  2:1864100/1‑150 (MQ=255)
                                                                                               ccGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAg                                                   <  1:7425432/150‑1 (MQ=255)
                                                                                               ccGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAg                                                   <  1:2300946/150‑1 (MQ=255)
                                                                                                 gAAGTGGCCGAACCGCGCCGAGCCCAGCGGGCGAGCGCGTAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAAGACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGaa                                                 <  2:8764237/150‑1 (MQ=255)
                                                                                                    gTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATc                                              <  2:9605656/150‑1 (MQ=255)
                                                                                                    gTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATc                                              >  1:4577450/1‑150 (MQ=255)
                                                                                                     tGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCg                                             >  2:1097228/1‑150 (MQ=255)
                                                                                                       gCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTa                                           >  1:11102716/1‑150 (MQ=255)
                                                                                                        ccGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAg                                          <  2:10493143/150‑1 (MQ=255)
                                                                                                         cGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGACATCGTAGa                                         >  2:8225875/1‑150 (MQ=255)
                                                                                                         cGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGa                                         <  2:4458460/150‑1 (MQ=255)
                                                                                                          gAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAATAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGaa                                        <  1:10486789/150‑1 (MQ=255)
                                                                                                           aaCAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAAt                                       <  2:6372760/150‑1 (MQ=255)
                                                                                                             cAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTc                                     >  1:8568411/1‑150 (MQ=255)
                                                                                                               gcgcCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGa                                   <  2:7052749/150‑1 (MQ=255)
                                                                                                                cgcCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGAt                                  >  2:839907/1‑150 (MQ=255)
                                                                                                                  ccGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATgg                                >  1:4463147/1‑150 (MQ=255)
                                                                                                                  ccGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATgg                                <  1:9975229/150‑1 (MQ=255)
                                                                                                                     aGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCgg                             <  2:4294545/150‑1 (MQ=255)
                                                                                                                       cccAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTg                           <  1:1020134/150‑1 (MQ=255)
                                                                                                                          aGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCg                        <  1:9826147/150‑1 (MQ=255)
                                                                                                                           gCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGa                       >  1:6843976/1‑150 (MQ=255)
                                                                                                                             gggCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATg                     <  2:10207441/150‑1 (MQ=255)
                                                                                                                                     gagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGc             <  1:6285407/150‑1 (MQ=255)
                                                                                                                                      agGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCg            >  1:3382798/1‑150 (MQ=255)
                                                                                                                                       ggAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGa           >  2:1523570/1‑150 (MQ=255)
                                                                                                                                           ggCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGc       <  1:786066/150‑1 (MQ=255)
                                                                                                                                            gCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCg      <  2:4110908/150‑1 (MQ=255)
                                                                                                                                               aTACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGAcc   >  1:2712139/1‑150 (MQ=255)
                                                                                                                                                tACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGAccc  <  1:9180747/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACGGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGACCC  >  NC_008463/1356401‑1356694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑